Mmp9 (NM_013599) Mouse Tagged ORF Clone

CAT#: MR226712

Mmp9 (Myc-DDK-tagged) - Mouse matrix metallopeptidase 9 (Mmp9)

Specifications

Product Data

• Type: Mouse Tagged ORF Clone
• Tag: Myc-DDK
• Symbol: Mmp9
• Synonyms: AW743869; B/MMP9; Clg4b; Gel B; MMP-9; pro-MMP-9
• Vector: pCMV6-Entry
• E. coli Selection: Kanamycin (25 ug/mL)
• Mammalian Cell Selection: Neomycin
• Sequence Data:
  ORF Nucleotide Sequence

  >MR226712 representing NM_013599
  Red=Cloning site Blue=ORF Green=Tags(s)
  
  TTTTGTAATACGACTCACTATAGGGCGGCCGGGAATTCGTCGACTGGATCCGGTACCGAGGAGATCTGCC
  GCCGCGATCGCC
  
  ATGAGTCCCTGGCAGCCCCTGCTCCTGGCTCTCCTGGCTTTCGGCTGCAGCTCTGCTGCCCCTTACCAGC
  GCCAGCCGACTTTTGTGGTCTTCCCCAAAGACCTGAAAACCTCCAACCTCACGGACACCCAGCTGGCAGA
  GGCATACTTGTACCGCTATGGTTACACCCGGGCCGCCCAGATGATGGGAGAGAAGCAGTCTCTACGGCCG
  GCTTTGCTGATGCTTCAGAAGCAGCTCTCCCTGCCCCAGACTGGTGAGCTGGACAGCCAGACACTAAAGG
  CCATTCGAACACCACGCTGTGGTGTCCCAGACGTGGGTCGATTCCAAACCTTCAAAGGCCTCAAGTGGGA
  CCATCATAACATCACATACTGGATCCAAAACTACTCTGAAGACTTGCCGCGAGACATGATCGATGACGCC
  TTCGCGCGCGCCTTCGCGGTGTGGGGCGAGGTGGCACCCCTCACCTTCACCCGCGTGTACGGACCCGAAG
  CGGACATTGTCATCCAGTTTGGTGTCGCGGAGCACGGAGACGGGTATCCCTTCGACGGCAAGGACGGCCT
  TCTGGCACACGCCTTTCCCCCTGGCGCCGGCGTTCAGGGAGATGCCCATTTCGACGACGACGAGTTGTGG
  TCGCTGGGCAAAGGCGTCGTGATCCCCACTTACTATGGAAACTCAAATGGTGCCCCATGTCACTTTCCCT
  TCACCTTCGAGGGACGCTCCTATTCGGCCTGCACCACAGACGGCCGCAACGACGGCACGCCTTGGTGTAG
  CACAACAGCTGACTACGATAAGGACGGCAAATTTGGTTTCTGCCCTAGTGAGAGACTCTACACGGAGCAC
  GGCAACGGAGAAGGCAAACCCTGTGTGTTCCCGTTCATCTTTGAGGGCCGCTCCTACTCTGCCTGCACCA
  CTAAAGGCCGCTCGGATGGTTACCGCTGGTGCGCCACCACAGCCAACTATGACCAGGATAAACTGTATGG
  CTTCTGCCCTACCCGAGTGGACGCGACCGTAGTTGGGGGCAACTCGGCAGGAGAGCTGTGCGTCTTCCCC
  TTCGTCTTCCTGGGCAAGCAGTACTCTTCCTGTACCAGCGACGGCCGCAGGGATGGGCGCCTCTGGTGTG
  CGACCACATCGAACTTCGACACTGACAAGAAGTGGGGTTTCTGTCCAGACCAAGGGTACAGCCTGTTCCT
  GGTGGCAGCGCACGAGTTCGGCCATGCACTGGGCTTAGATCATTCCAGCGTGCCGGAAGCGCTCATGTAC
  CCGCTGTATAGCTACCTCGAGGGCTTCCCTCTGAATAAAGACGACATAGACGGCATCCAGTATCTGTATG
  GTCGTGGCTCTAAGCCTGACCCAAGGCCTCCAGCCACCACCACAACTGAACCACAGCCGACAGCACCTCC
  CACTATGTGTCCCACTATACCTCCCACGGCCTATCCCACAGTGGGCCCCACGGTTGGCCCTACAGGCGCC
  CCCTCACCTGGCCCCACAAGCAGCCCGTCACCTGGCCCTACAGGCGCCCCCTCACCTGGCCCTACAGCGC
  CCCCTACTGCGGGCTCTTCTGAGGCCTCTACAGAGTCTTTGAGTCCGGCAGACAATCCTTGCAATGTGGA
  TGTTTTTGATGCTATTGCTGAGATCCAGGGCGCTCTGCATTTCTTCAAGGACGGTTGGTACTGGAAGTTC
  CTGAATCATAGAGGAAGCCCATTACAGGGCCCCTTCCTTACTGCCCGCACGTGGCCAGCCCTGCCTGCAA
  CGCTGGACTCCGCCTTTGAGGATCCGCAGACCAAGAGGGTTTTCTTCTTCTCTGGACGTCAAATGTGGGT
  GTACACAGGCAAGACCGTGCTGGGCCCCAGGAGTCTGGATAAGTTGGGTCTAGGCCCAGAGGTAACCCAC
  GTCAGCGGGCTTCTCCCGCGTCGTCTCGGGAAGGCTCTGCTGTTCAGCAAGGGGCGTGTCTGGAGATTCG
  ACTTGAAGTCTCAGAAGGTGGATCCCCAGAGCGTCATTCGCGTGGATAAGGAGTTCTCTGGTGTGCCCTG
  GAACTCACACGACATCTTCCAGTACCAAGACAAAGCCTATTTCTGCCATGGCAAATTCTTCTGGCGTGTG
  AGTTTCCAAAATGAGGTGAACAAGGTGGACCATGAGGTGAACCAGGTGGACGACGTGGGCTACGTGACCT
  ACGACCTCCTGCAGTGCCCT
  
  ACGCGTACGCGGCCGCTCGAGCAGAAACTCATCTCAGAAGAGGATCTGGCAGCAAATGATATCCTGGATT
  ACAAGGATGACGACGATAAGGTTTAA
  Protein Sequence

  >MR226712 representing NM_013599
  Red=Cloning site Green=Tags(s)
  
  MSPWQPLLLALLAFGCSSAAPYQRQPTFVVFPKDLKTSNLTDTQLAEAYLYRYGYTRAAQMMGEKQSLRP
  ALLMLQKQLSLPQTGELDSQTLKAIRTPRCGVPDVGRFQTFKGLKWDHHNITYWIQNYSEDLPRDMIDDA
  FARAFAVWGEVAPLTFTRVYGPEADIVIQFGVAEHGDGYPFDGKDGLLAHAFPPGAGVQGDAHFDDDELW
  SLGKGVVIPTYYGNSNGAPCHFPFTFEGRSYSACTTDGRNDGTPWCSTTADYDKDGKFGFCPSERLYTEH
  GNGEGKPCVFPFIFEGRSYSACTTKGRSDGYRWCATTANYDQDKLYGFCPTRVDATVVGGNSAGELCVFP
  FVFLGKQYSSCTSDGRRDGRLWCATTSNFDTDKKWGFCPDQGYSLFLVAAHEFGHALGLDHSSVPEALMY
  PLYSYLEGFPLNKDDIDGIQYLYGRGSKPDPRPPATTTTEPQPTAPPTMCPTIPPTAYPTVGPTVGPTGA
  PSPGPTSSPSPGPTGAPSPGPTAPPTAGSSEASTESLSPADNPCNVDVFDAIAEIQGALHFFKDGWYWKF
  LNHRGSPLQGPFLTARTWPALPATLDSAFEDPQTKRVFFFSGRQMWVYTGKTVLGPRSLDKLGLGPEVTH
  VSGLLPRRLGKALLFSKGRVWRFDLKSQKVDPQSVIRVDKEFSGVPWNSHDIFQYQDKAYFCHGKFFWRV
  SFQNEVNKVDHEVNQVDDVGYVTYDLLQCP
  
  TRTRPLEQKLISEEDLAANDILDYKDDDDKV
• Restriction Sites: SgfI-MluI Cloning Scheme for this gene Plasmid Map
• ACCN: NM_013599
• ORF Size: 2190 bp
• OTI Disclaimer: The molecular sequence of this clone aligns with the gene accession number as a point of reference only. However, individual transcript sequences of the same gene can differ through naturally occurring variations (e.g. polymorphisms), each with its own valid existence. This clone is substantially in agreement with the reference, but a complete review of all prevailing variants is recommended prior to use. More info
• OTI Annotation: This clone was engineered to express the complete ORF with an expression tag. Expression varies depending on the nature of the gene.

Reference Data

• RefSeq: NM_013599.1, NM_013599.2, NM_013599.3, NM_013599.4, NP_038627.1
• RefSeq Size: 3185
• RefSeq ORF: 2193
• Locus ID: 17395
• MW: 81 kDa
• Gene Summary: This gene encodes a member of the matrix metalloproteinase family of extracellular matrix-degrading enzymes that are involved in tissue remodeling, wound repair, progression of atherosclerosis and tumor invasion. The encoded preproprotein undergoes proteolytic processing to generate a mature, zinc-dependent endopeptidase enzyme that degrades collagens of type IV, V and XI, and elastin. Mice lacking the encoded protein exhibit an abnormal pattern of skeletal growth plate vascularization and ossification, reduced keratinocyte hyperproliferation at all neoplastic stages, a decreased incidence of invasive tumors, and resistance to experimental autoimmune encephalomyelitis. [provided by RefSeq, Feb 2016]