Ikbkb (NM_010546) Mouse Tagged ORF Clone

CAT#: MR227004

Ikbkb (Myc-DDK-tagged) - Mouse inhibitor of kappaB kinase beta (Ikbkb), transcript variant 2

Specifications

Product Data

• Type: Mouse Tagged ORF Clone
• Tag: Myc-DDK
• Symbol: Ikbkb
• Synonyms: AI132552; IKK-2; IKK-beta; IKK2; IKKbeta; IKK[b]
• Vector: pCMV6-Entry
• E. coli Selection: Kanamycin (25 ug/mL)
• Mammalian Cell Selection: Neomycin
• Sequence Data:
  ORF Nucleotide Sequence

  >MR227004 representing NM_010546
  Red=Cloning site Blue=ORF Green=Tags(s)
  
  TTTTGTAATACGACTCACTATAGGGCGGCCGGGAATTCGTCGACTGGATCCGGTACCGAGGAGATCTGCC
  GCCGCGATCGCC
  
  ATGAGCTGGTCACCGTCCCTCCCAACCCAGACATGTGGAGCCTGGGAAATGAAAGAACGCCTGGGGACCG
  GGGGATTTGGAAACGTCATCCGGTGGCACAATCAGGCGACAGGTGAACAGATCGCCATCAAGCAATGCCG
  ACAGGAGCTCAGCCCAAAGAACAGAGACCGCTGGTGCCTCGAAATCCAGATCATGAGAAGGCTGAACCAT
  CCCAATGTGGTGGCTGCCCGGGATGTCCCAGAGGGGATGCAGAACCTGGCACCCAATGATTTGCCACTGC
  TGGCCATGGAGTACTGCCAAGGAGGAGATCTCCGAAGATACTTGAACCAGTTCGAGAACTGCTGTGGCCT
  GCGGGAAGGAGCTGTCCTTACCCTGCTGAGTGACATCGCATCGGCTCTTAGATACCTTCACGAAAACAGA
  ATCATCCATCGAGACCTGAAGCCAGAAAACATCGTTCTGCAGCAAGGAGAGAAAAGATTAATACACAAAA
  TTATTGATCTAGGATATGCCAAGGAGCTGGATCAGGGCAGTCTGTGCACGTCATTTGTGGGGACTCTGCA
  ATACCTGGCGCCAGAGCTTCTGGAGCAGCAGAAGTACACCGTGACCGTTGACTACTGGAGCTTCGGCACC
  CTGGCCTTCGAGTGCATCACTGGCTTCCGGCCCTTCCTCCCTAACTGGCAGCCTGTGCAGTGGCACTCCA
  AAGTCCGGCAGAAGAGCGAAGTGGACATCGTTGTTAGTGAAGACTTGAATGGAGCAGTGAAGTTTTCAAG
  TTCGCTACCCTTCCCCAATAATCTTAACAGTGTCTTGGCTGAACGGCTGGAGAAGTGGCTGCAGCTGATG
  CTTATGTGGCACCCTCGGCAAAGGGGCACGGATCCCCAGTATGGCCCCAACGGCTGCTTCAGAGCCCTGG
  ATGACATCTTGAACTTGAAGCTGGTTCATGTCTTGAACATGGTCACAGGCACCGTTCACACATACCCCGT
  GACGGAGGATGAGAGTCTGCAGAGCTTAAAAACCAGAATCCAGGAAGACACGGGGATCCTGGAGACAGAC
  CAGGAGCTGCTGCAAGAGGCAGGGCTGGTGCTGCTCCCTGACAAGCCTGCTACTCAGTGCATCTCAGACA
  GCAAGACAAACGAGGGCCTCACGTTGGACATGGATCTTGTTTTTCTCTTTGACAACAGTAAAATCAACTA
  TGAGACTCAGATCACCCCCCGACCCCAACCGGAAAGTGTCAGCTGTATCCTTCAGGAGCCCAAGCGGAAC
  CTCTCCTTCTTCCAGCTGAGGAAAGTGTGGGGCCAAGTCTGGCACAGCATCCAGACGCTGAAGGAAGACT
  GTAACCGGCTGCAGCAGGGACAGCGAGCAGCCATGATGAGTCTCCTCCGGAATAACAGCTGCCTCTCTAA
  GATGAAGAACGCCATGGCCTCCACGGCCCAGCAGCTCAAGGCCAAGCTGGACTTCTTCAAAACCAGCATC
  CAGATCGACCTGGAGAAGTATAAAGAGCAGACCGAGTTTGGGATCACCTCAGATAAATTGCTGCTGGCTT
  GGCGGGAGATGGAGCAGGCTGTGGAGCAGTGTGGGCGGGAGAATGACGTGAAGCATCTAGTAGAGCGGAT
  GATGGCACTGCAGACTGACATTGTGGACCTGCAGAGGAGCCCGATGGGTCGGAAGCAGGGGGGCACCCTG
  GATGACCTAGAGGAACAAGCGAGGGAGCTCTACCGAAGACTCAGGGAGAAGCCAAGAGACCAAAGGACAG
  AAGGTGACAGCCAGGAGATGGTACGGCTGCTGCTTCAGGCAATCCAAAGCTTTGAGAAGAAAGTTCGGGT
  GATTTATACACAGCTCAGTAAGACCGTGGTTTGTAAGCAGAAGGCACTGGAGTTGCTGCCCAAGGTAGAA
  GAGGTAGTGAGCCTTATGAACGAGGACGAGAGGACCGTGGTCCGGCTTCAGGAGAAGCGGCAGAAGGAAC
  TCTGGAACCTCCTGAAGATCGCCTGTAGCAAAGTCCGAGGTCCCGTGAGTGGAAGCCCAGACAGCATGAA
  TGTGTCTCGACTCAGTCACCCTGGTCAGCTAATGTCCCAGCCTTCCAGTGCCTGTGACAGCTTACCTGAA
  TCAGACAAGAAAAGTGAAGAACTGGTGGCCGAAGCCCACGCCCTCTGCTCCCGGCTAGAAAGTGCGCTGC
  AGGACACTGTGAAGGAGCAAGACAGAAGCTTCACGGTAACCGCC
  
  ACGCGTACGCGGCCGCTCGAGCAGAAACTCATCTCAGAAGAGGATCTGGCAGCAAATGATATCCTGGATT
  ACAAGGATGACGACGATAAGGTTTAA
  Protein Sequence

  >MR227004 representing NM_010546
  Red=Cloning site Green=Tags(s)
  
  MSWSPSLPTQTCGAWEMKERLGTGGFGNVIRWHNQATGEQIAIKQCRQELSPKNRDRWCLEIQIMRRLNH
  PNVVAARDVPEGMQNLAPNDLPLLAMEYCQGGDLRRYLNQFENCCGLREGAVLTLLSDIASALRYLHENR
  IIHRDLKPENIVLQQGEKRLIHKIIDLGYAKELDQGSLCTSFVGTLQYLAPELLEQQKYTVTVDYWSFGT
  LAFECITGFRPFLPNWQPVQWHSKVRQKSEVDIVVSEDLNGAVKFSSSLPFPNNLNSVLAERLEKWLQLM
  LMWHPRQRGTDPQYGPNGCFRALDDILNLKLVHVLNMVTGTVHTYPVTEDESLQSLKTRIQEDTGILETD
  QELLQEAGLVLLPDKPATQCISDSKTNEGLTLDMDLVFLFDNSKINYETQITPRPQPESVSCILQEPKRN
  LSFFQLRKVWGQVWHSIQTLKEDCNRLQQGQRAAMMSLLRNNSCLSKMKNAMASTAQQLKAKLDFFKTSI
  QIDLEKYKEQTEFGITSDKLLLAWREMEQAVEQCGRENDVKHLVERMMALQTDIVDLQRSPMGRKQGGTL
  DDLEEQARELYRRLREKPRDQRTEGDSQEMVRLLLQAIQSFEKKVRVIYTQLSKTVVCKQKALELLPKVE
  EVVSLMNEDERTVVRLQEKRQKELWNLLKIACSKVRGPVSGSPDSMNVSRLSHPGQLMSQPSSACDSLPE
  SDKKSEELVAEAHALCSRLESALQDTVKEQDRSFTVTA
  
  TRTRPLEQKLISEEDLAANDILDYKDDDDKV
• Restriction Sites: SgfI-MluI Cloning Scheme for this gene Plasmid Map
• ACCN: NM_010546
• ORF Size: 2214 bp
• OTI Disclaimer: Due to the inherent nature of this plasmid, standard methods to replicate additional amounts of DNA in E. coli are highly likely to result in mutations and/or rearrangements. Therefore, OriGene does not guarantee the capability to replicate this plasmid DNA. Additional amounts of DNA can be purchased from OriGene with batch-specific, full-sequence verification at a reduced cost. Please contact our customer care team at custsupport@origene.com or by calling 301.340.3188 option 3 for pricing and delivery.
  
  The molecular sequence of this clone aligns with the gene accession number as a point of reference only. However, individual transcript sequences of the same gene can differ through naturally occurring variations (e.g. polymorphisms), each with its own valid existence. This clone is substantially in agreement with the reference, but a complete review of all prevailing variants is recommended prior to use. More info
• OTI Annotation: This clone was engineered to express the complete ORF with an expression tag. Expression varies depending on the nature of the gene.

Reference Data

• RefSeq: NM_010546.1, NM_010546.2, NP_034676.1
• RefSeq Size: 4540
• RefSeq ORF: 2217
• Locus ID: 16150
• MW: 84.8 kDa