Ehmt1 (NM_001012518) Mouse Tagged ORF Clone

CAT#: MR227091

Ehmt1 (Myc-DDK-tagged) - Mouse euchromatic histone methyltransferase 1 (Ehmt1), transcript variant 1

Specifications

Product Data

• Type: Mouse Tagged ORF Clone
• Tag: Myc-DDK
• Symbol: Ehmt1
• Synonyms: 9230102N17Rik; D330003E03; Eu-HMTase1; GLP; GLP1; KMT1D; mKIAA1876
• Vector: pCMV6-Entry
• E. coli Selection: Kanamycin (25 ug/mL)
• Mammalian Cell Selection: Neomycin
• Sequence Data:
  ORF Nucleotide Sequence

  >MR227091 representing NM_001012518
  Red=Cloning site Blue=ORF Green=Tags(s)
  
  TTTTGTAATACGACTCACTATAGGGCGGCCGGGAATTCGTCGACTGGATCCGGTACCGAGGAGATCTGCC
  GCCGCGATCGCC
  
  ATGGCCGCCGCTGATGCTGAGCAGGCAGTTCTGGCCAAGCAAGAGACCAAGCAGGATTGCTGCATGAAAA
  CTGAGCTGCTAAGGGAAGATACACCTATGGCTGCTGATGAAGGTTCCACAGAGAAACAAGAAGGAGAGAC
  TCCCATGGCTGCAGATGGAGAAACAAATGGGTCTTGTGAAAAGAGTGGGGATCCCAGCCATCTAAATGCA
  CCCAAACACACTCAGGAGAACACAAGAGCTAGCCCACAGGAAGGCACCAACAGAGTGTCTCGGGTGGCAG
  AAAATGGGGTTTCAGAAAGAGACACAGAAGTGGGGAAGCAAAACCATGTCACAGCTGACGACTTCATGCA
  GACATCTGTCATTGGCAGCAATGGATATTTCTTAAATAAACCAGCCCTGCAGGGGCAGCCGTTGAGGACT
  CCCAACATTCTAACCTCCTCGCTTCCTGGTCATGCTGCAAAAACTCTTCCTGGAGGAGCCAGTAAATGCA
  GGACTCTGAGTGCACTTCCTCAGACACCAACCACAGCACCCACTGTGCCTGGGGAAGGGAGTGCAGACAC
  AGAGGACAGAAAGCCTACAGCCTCGGGCACTGATGTCAGGGTTCACAGGGCACGCAAGACCATGCCGAAG
  TCCATCTTGGGCCTGCATGCAGCCAGCAAAGACCATAGAGAAGTTCAAGACCATAAGGAACCAAAAGAGG
  ATATCAACAGAAACATTTCTGAATGTGGACGACAGCAGCTTTTACCAACCTTCCCAGCCCTCCACCAGTC
  GCTACCTCAGAATCAGTGCTACATGGCCACCACAAAGTCCCAGACAGCTTGCTTGCCTTTTGTTTTAGCA
  GCTGCAGTATCTCGGAAGAAAAAACGAAGAATGGGAACCTATAGTTTAGTTCCCAAGAAAAAGACCAAAG
  TATTAAAACAGAGGACGGTGATTGAGATGTTTAAAAGCATAACCCATTCCACTGTGGGCGCCAAGGGCGA
  GAAAGCCTTAGATGATAGTGCCCTGCATGTAAATGGCGAGAGCTTGGAGATGGACTCAGAAGATGAAGAC
  TCCGATGAGCTGGAGGATGACGAGGACCATGGAGCTGAGCAAGCGGCTGCATTTCCCACCGAGGATAGTA
  GGACTTCTAAAGAGAGCATGTCTGAGACTGACCGGGCTGCAAAGATGGATGGAGATTCAGAGGAGGAACA
  GGAGTCTCCCGACACAGGGGAGGATGAAGATGGTGGAGACGAGTCTGACCTGAGTTCTGAATCCAGTATC
  AAGAAGAAATTTCTCAAGAGGAGAGGGAAGACTGACAGCCCCTGGATCAAACCTGCTCGGAAAAGGAGGC
  GGAGAAGTAGAAAGAAGCCGAGCAGCATGCTTGGTTCCGAGGCTTGTAAGTCATCTCCAGGAAGCATGGA
  GCAGGCAGCTCTGGGAGACAGCGCTGGCTATATGGAAGTTTCCCTGGATTCCCTGGATCTCCGTGTCAGA
  GGAATTCTGTCTTCCCAGACAGAAAATGAAGGGCTGGCCAGTGGTCCGGATGTGCTGGGGACGGATGGCC
  TCCAGGAAGTGCCTCTCTGCAGCTGCCGAATGGAAACCCCCAAGAGCCGCGAGATCAGCACCCTGGCCAA
  CAACCAGTGCATGGCCACTGAGAGCGTGGATCACGAATTGGGCCGGTGTACAAACAGCGTGGTCAAGTAT
  GAGCTGATGCGCCCATCCAACAAAGCACCGCTCTTGGTGTTATGTGAAGACCATCGGGGTCGCATGGTGA
  AGCACCAGTGCTGTCCTGGCTGCGGCTACTTCTGCACGGCGGGTAACTTCATGGAATGCCAGCCTGAGAG
  CAGCATCTCTCATCGTTTCCATAAGGACTGTGCCTCTCGAGTCAACAATGCCAGCTACTGTCCCCATTGT
  GGGGAAGAAGCTTCCAAGGCCAAAGAGGTGACCATAGCAAAAGCAGACACAACTTCCACAGTGACCCTAG
  CCCCTGGACAGGAGAAGAGCCTGGCTGCTGAAGGCAGGGCTGACACGACCACGGGCAGCATTGCTGGAGC
  CCCAGAGGATGAAAGATCGCAGAGCACAGCCCCCCAGGCACCAGAGTGCTTCGACCCTGCCGGACCGGCT
  GGGCTCGTGAGGCCGACATCTGGCCTTTCCCAGGGCCCAGGAAAGGAAACCTTGGAAAGTGCTCTAATCG
  CTCTAGACTCTGAAAAACCCAAGAAACTTCGCTTCCACCCAAAGCAGCTGTACTTCTCTGCCAGGCAGGG
  TGAGCTGCAGAAGGTGCTTCTCATGCTGGTTGATGGAATTGATCCCAACTTCAAAATGGAGCACCAAAGT
  AAGCGTTCCCCATTACATGCTGCTGCGGAGGCTGGCCACGTGGACATCTGCCACATGCTGGTTCAGGCGG
  GTGCCAATATTGACACTTGCTCAGAGGACCAGCGGACCCCACTGATGGAGGCTGCAGAGAACAACCACTT
  GGATGCAGTGAAGTACCTCATCAAGGCTGGAGCACAGGTGGATCCGAAGGACGCAGAGGGCTCCACATGT
  TTGCATTTGGCTGCCAAGAAAGGCCACTATGATGTGGTTCAGTATCTGCTTTCAAATGGACAGATGGATG
  TCAACTGCCAGGATGACGGTGGATGGACACCTATGATCTGGGCCACTGAGTACAAGCACGTGGAGCTGGT
  GAAGCTGCTGCTGTCTAAGGGCTCTGACATCAACATCCGGGACAACGAGGAGAACATTTGTCTGCACTGG
  GCAGCATTTTCAGGCTGTGTGGACATAGCTGAAATACTTCTGGCTGCCAAGTGTGACCTGCATGCTGTGA
  ATATCCATGGAGACTCACCCCTGCACATCGCAGCCAGGGAGAATCGCTACGACTGTGTTGTCCTCTTTCT
  TTCTCGGGATTCAGATGTTACTCTGAAAAACAAGGAAGGAGAGACTCCCTTGCAGTGTGCAAGTCTCAGT
  TCGCAGGTGTGGAGTGCATTGCAGATGAGCAAAGCACTTCGGGACTCAGCCCCTGACAAGCCCGTTGCTG
  TTGAGAAGACGGTGAGCAGGGATATCGCTCGAGGGTATGAGCGCATTCCCATTCCCTGTGTCAATGCTGT
  GGACAGTGAGCTGTGTCCTACCAACTATAAGTATGTCTCCCAGAACTGTGTGACATCCCCCATGAACATT
  GACAGGAACATCACTCATTTGCAGTACTGCGTGTGTGTAGATGACTGCTCCTCTAGCACCTGCATGTGTG
  GCCAGCTGAGCATGCGCTGCTGGTATGATAAGGATGGCCGACTTCTGCCTGAGTTTAACATGGCAGAACC
  ACCCTTGATCTTCGAGTGCAATCATGCCTGCTCATGCTGGAGGAACTGCCGCAATCGTGTGGTGCAAAAT
  GGTCTCAGGGCAAGGCTGCAGCTTTATCGGACACAGGACATGGGCTGGGGTGTGCGGTCCCTCCAGGATA
  TCCCACTGGGCACCTTTGTCTGCGAATACGTAGGGGAGCTGATTTCGGACTCTGAAGCTGATGTTCGGGA
  AGAGGACTCTTACCTCTTTGATCTTGACAATAAGGATGGAGAGGTATACTGCATTGACGCTCGGTTCTAT
  GGGAATGTCAGCCGGTTCATAAACCACCACTGCGAACCCAACCTTGTGCCTGTGCGAGTGTTCATGTCAC
  ACCAGGACCTGCGGTTTCCCAGGATTGCCTTCTTCAGTACCCGCCTGATTCAGGCTGGGGAGCAGCTCGG
  GTTCGACTACGGGGAGCGCTTTTGGGACGTCAAGGGCAAGCTCTTCAGTTGCCGGTGTGGGTCTTCCAAG
  TGTCGGCACTCAAGCGCAGCCCTGGCCCAGAGGCAAGCCAGTGCAGCCCAGGAGCCTCAGGAGAATGGCC
  TTCCAGATACCAGCTCTGCAGCCGCTGCTGACCCCCTA
  
  ACGCGTACGCGGCCGCTCGAGCAGAAACTCATCTCAGAAGAGGATCTGGCAGCAAATGATATCCTGGATT
  ACAAGGATGACGACGATAAGGTTTAA
  Protein Sequence

  >MR227091 representing NM_001012518
  Red=Cloning site Green=Tags(s)
  
  MAAADAEQAVLAKQETKQDCCMKTELLREDTPMAADEGSTEKQEGETPMAADGETNGSCEKSGDPSHLNA
  PKHTQENTRASPQEGTNRVSRVAENGVSERDTEVGKQNHVTADDFMQTSVIGSNGYFLNKPALQGQPLRT
  PNILTSSLPGHAAKTLPGGASKCRTLSALPQTPTTAPTVPGEGSADTEDRKPTASGTDVRVHRARKTMPK
  SILGLHAASKDHREVQDHKEPKEDINRNISECGRQQLLPTFPALHQSLPQNQCYMATTKSQTACLPFVLA
  AAVSRKKKRRMGTYSLVPKKKTKVLKQRTVIEMFKSITHSTVGAKGEKALDDSALHVNGESLEMDSEDED
  SDELEDDEDHGAEQAAAFPTEDSRTSKESMSETDRAAKMDGDSEEEQESPDTGEDEDGGDESDLSSESSI
  KKKFLKRRGKTDSPWIKPARKRRRRSRKKPSSMLGSEACKSSPGSMEQAALGDSAGYMEVSLDSLDLRVR
  GILSSQTENEGLASGPDVLGTDGLQEVPLCSCRMETPKSREISTLANNQCMATESVDHELGRCTNSVVKY
  ELMRPSNKAPLLVLCEDHRGRMVKHQCCPGCGYFCTAGNFMECQPESSISHRFHKDCASRVNNASYCPHC
  GEEASKAKEVTIAKADTTSTVTLAPGQEKSLAAEGRADTTTGSIAGAPEDERSQSTAPQAPECFDPAGPA
  GLVRPTSGLSQGPGKETLESALIALDSEKPKKLRFHPKQLYFSARQGELQKVLLMLVDGIDPNFKMEHQS
  KRSPLHAAAEAGHVDICHMLVQAGANIDTCSEDQRTPLMEAAENNHLDAVKYLIKAGAQVDPKDAEGSTC
  LHLAAKKGHYDVVQYLLSNGQMDVNCQDDGGWTPMIWATEYKHVELVKLLLSKGSDINIRDNEENICLHW
  AAFSGCVDIAEILLAAKCDLHAVNIHGDSPLHIAARENRYDCVVLFLSRDSDVTLKNKEGETPLQCASLS
  SQVWSALQMSKALRDSAPDKPVAVEKTVSRDIARGYERIPIPCVNAVDSELCPTNYKYVSQNCVTSPMNI
  DRNITHLQYCVCVDDCSSSTCMCGQLSMRCWYDKDGRLLPEFNMAEPPLIFECNHACSCWRNCRNRVVQN
  GLRARLQLYRTQDMGWGVRSLQDIPLGTFVCEYVGELISDSEADVREEDSYLFDLDNKDGEVYCIDARFY
  GNVSRFINHHCEPNLVPVRVFMSHQDLRFPRIAFFSTRLIQAGEQLGFDYGERFWDVKGKLFSCRCGSSK
  CRHSSAALAQRQASAAQEPQENGLPDTSSAAAADPL
  
  TRTRPLEQKLISEEDLAANDILDYKDDDDKV
• Restriction Sites: SgfI-MluI Cloning Scheme for this gene Plasmid Map
• ACCN: NM_001012518
• ORF Size: 3888 bp
• OTI Disclaimer: The molecular sequence of this clone aligns with the gene accession number as a point of reference only. However, individual transcript sequences of the same gene can differ through naturally occurring variations (e.g. polymorphisms), each with its own valid existence. This clone is substantially in agreement with the reference, but a complete review of all prevailing variants is recommended prior to use. More info
• OTI Annotation: This clone was engineered to express the complete ORF with an expression tag. Expression varies depending on the nature of the gene.
• Product Components: The ORF clone is ion-exchange column purified, transfection-ready dried plasmid DNA, and shipped with 2 vector sequencing primers.
• Reconstitution: 1. Centrifuge at 5,000xg for 5min.
  2. Carefully open the tube and add 100ul of sterile water to dissolve the DNA.
  3. Close the tube and incubate for 10 minutes at room temperature.
  4. Briefly vortex the tube and then do a quick spin (less than 5000xg) to concentrate the liquid at the bottom.
  5. Store the suspended plasmid at -20°C. The DNA is stable for at least one year from date of shipping when stored at -20°C.

Reference Data

• RefSeq: NM_001012518.1, NM_001012518.2, NM_001012518.3, NP_001012536.2
• RefSeq Size: 5091 bp
• RefSeq ORF: 3891 bp
• Locus ID: 77683
• Cytogenetics: 2 A3
• MW: 142.4 kDa