Itk (NM_010583) Mouse Tagged ORF Clone

CAT#: MR227110

Itk (Myc-DDK-tagged) - Mouse IL2-inducible T-cell kinase (Itk)

Specifications

Product Data

• Type: Mouse Tagged ORF Clone
• Tag: Myc-DDK
• Symbol: Itk
• Synonyms: Emt; Tcsk; Tsk
• Vector: pCMV6-Entry
• E. coli Selection: Kanamycin (25 ug/mL)
• Mammalian Cell Selection: Neomycin
• Sequence Data:
  ORF Nucleotide Sequence

  >MR227110 representing NM_010583
  Red=Cloning site Blue=ORF Green=Tags(s)
  
  TTTTGTAATACGACTCACTATAGGGCGGCCGGGAATTCGTCGACTGGATCCGGTACCGAGGAGATCTGCC
  GCCGCGATCGCC
  
  ATGAACAACTTCATCCTCCTGGAAGAACAGCTGATCAAGAAGTCCCAACAGAAGAGAAGGACTTCTCCCT
  CGAATTTTAAAGTTCGTTTCTTTGTCTTAACGAAAGCCAGCCTGGCCTACTTTGAGGACCGCCACGGGAA
  GAAGCGCACGTTGAAGGGCTCCATTGAACTCTCCAGAATCAAGTGTGTGGAGATTGTCAAGAGTGACATT
  AGCATCCCGTGCCACTATAAATACCCTTTTCAGGTCGTGCATGACAACTATCTCCTGTATGTGTTTGCTC
  CAGACTGTGAGAGTCGGCAGCGCTGGGTGCTGACCCTTAAAGAAGAAACGAGGAATAACAACAGCCTGGT
  ATCCAAGTATCACCCTAATTTCTGGATGGATGGGCGGTGGAGGTGCTGCTCCCAGCTGGAGAAGCCTGCT
  GTAGGCTGTGCTCCCTACGACCCATCCAAGAATGCTTCAAAGAAGCCTCTTCCTCCTACTCCTGAAGACA
  ACAGGCGGTCATTTCAGGAACCTGAAGAAACCCTGGTCATTGCCTTGTACGACTACCAAACCAACGACCC
  TCAGGAGCTCGCACTGCGGTGTGATGAAGAGTACTACCTGCTGGACAGCTCCGAGATCCACTGGTGGAGG
  GTTCAAGACAAAAATGGGCATGAAGGATATGCACCAAGCAGTTACCTGGTAGAAAAATCTCCAAATAACC
  TTGAAACCTATGAGTGGTACAATAAAAGCATCAGCCGCGACAAAGCTGAAAAACTTCTTTTGGACACAGG
  TAAAGAAGGAGCTTTCATGGTCCGAGATTCCAGGACACCCGGGACATACACAGTCTCTGTTTTCACCAAG
  GCCATCATAAGTGAGAACCCCTGTATAAAACATTATCACATCAAAGAAACAAATGACAGCCCCAAGCGCT
  ACTACGTGGCTGAGAAGTATGTGTTTGACTCCATCCCTCTCCTCATCCAGTATCACCAGTACAATGGAGG
  AGGTTTGGTCACTCGACTCCGCTATCCAGTTTGCTCCTGGAGACAAAAAGCCCCTGTCACAGCAGGGCTA
  AGATATGGGAAGTGGGTGATCCAACCCTCAGAGCTAACGTTCGTGCAGGAGATTGGCAGCGGGCAGTTTG
  GGCTGGTGCATCTCGGCTACTGGCTCAACAAGGACAAGGTGGCCATCAAGACCATTCAGGAAGGGGCGAT
  GTCAGAAGAAGACTTTATCGAGGAGGCGGAAGTCATGATGAAACTCTCTCACCCCAAACTGGTGCAGCTC
  TATGGGGTGTGCCTGGAGCAAGCCCCCATCTGCCTGGTGTTTGAGTTCATGGAGCACGGCTGCCTGTCGG
  ATTACCTTCGAAGTCAGCGGGGTCTCTTTGCTGCGGAGACCCTGCTGGGCATGTGCCTGGATGTGTGTGA
  GGGCATGGCCTACCTGGAAAAAGCTTGTGTCATCCACAGAGACCTGGCAGCCAGAAACTGTTTGGTGGGA
  GAAAACCAGGTCATCAAGGTGTCCGACTTTGGGATGACAAGATTTGTCCTTGATGATCAATATACCAGCT
  CCACGGGCACCAAATTCCCAGTGAAGTGGGCATCCCCAGAGGTGTTCTCCTTTAGTCGCTATAGCAGCAA
  GTCAGATGTGTGGTCGTTTGGTGTACTGATGTGGGAAGTCTTCAGTGAAGGCAAAATCCCATACGAAAAC
  CGAAGCAATTCCGAGGTCGTGGAAGATATCAGCACTGGCTTTCGGTTATACAAGCCCCGCCTGGCCTCCT
  GTCATGTCTACCAGATCATGAATCATTGCTGGAAAGAGAAACCAGAAGACCGGCCACCCTTCTCCCAGCT
  GTTAAGCCAGCTGGCTGAAATCGCAGAAGCTGGGCTT
  
  ACGCGTACGCGGCCGCTCGAGCAGAAACTCATCTCAGAAGAGGATCTGGCAGCAAATGATATCCTGGATT
  ACAAGGATGACGACGATAAGGTTTAA
  Protein Sequence

  >MR227110 representing NM_010583
  Red=Cloning site Green=Tags(s)
  
  MNNFILLEEQLIKKSQQKRRTSPSNFKVRFFVLTKASLAYFEDRHGKKRTLKGSIELSRIKCVEIVKSDI
  SIPCHYKYPFQVVHDNYLLYVFAPDCESRQRWVLTLKEETRNNNSLVSKYHPNFWMDGRWRCCSQLEKPA
  VGCAPYDPSKNASKKPLPPTPEDNRRSFQEPEETLVIALYDYQTNDPQELALRCDEEYYLLDSSEIHWWR
  VQDKNGHEGYAPSSYLVEKSPNNLETYEWYNKSISRDKAEKLLLDTGKEGAFMVRDSRTPGTYTVSVFTK
  AIISENPCIKHYHIKETNDSPKRYYVAEKYVFDSIPLLIQYHQYNGGGLVTRLRYPVCSWRQKAPVTAGL
  RYGKWVIQPSELTFVQEIGSGQFGLVHLGYWLNKDKVAIKTIQEGAMSEEDFIEEAEVMMKLSHPKLVQL
  YGVCLEQAPICLVFEFMEHGCLSDYLRSQRGLFAAETLLGMCLDVCEGMAYLEKACVIHRDLAARNCLVG
  ENQVIKVSDFGMTRFVLDDQYTSSTGTKFPVKWASPEVFSFSRYSSKSDVWSFGVLMWEVFSEGKIPYEN
  RSNSEVVEDISTGFRLYKPRLASCHVYQIMNHCWKEKPEDRPPFSQLLSQLAEIAEAGL
  
  TRTRPLEQKLISEEDLAANDILDYKDDDDKV
• Restriction Sites: SgfI-MluI Cloning Scheme for this gene Plasmid Map
• ACCN: NM_010583
• ORF Size: 1857 bp
• OTI Disclaimer: The molecular sequence of this clone aligns with the gene accession number as a point of reference only. However, individual transcript sequences of the same gene can differ through naturally occurring variations (e.g. polymorphisms), each with its own valid existence. This clone is substantially in agreement with the reference, but a complete review of all prevailing variants is recommended prior to use. More info
• OTI Annotation: This clone was engineered to express the complete ORF with an expression tag. Expression varies depending on the nature of the gene.

Reference Data

• RefSeq: NM_010583.2, NM_010583.3, NP_034713.2
• RefSeq Size: 4284
• RefSeq ORF: 1860
• Locus ID: 16428
• MW: 72 kDa