Nck2 (NM_010879) Mouse Tagged ORF Clone

CAT#: MR227128

Nck2 (Myc-DDK-tagged) - Mouse non-catalytic region of tyrosine kinase adaptor protein 2 (Nck2)

Specifications

Product Data

• Type: Mouse Tagged ORF Clone
• Tag: Myc-DDK
• Symbol: Nck2
• Synonyms: 4833426I10Rik; Grb4; Nck-2; NCKbeta
• Vector: pCMV6-Entry
• E. coli Selection: Kanamycin (25 ug/mL)
• Mammalian Cell Selection: Neomycin
• Sequence Data:
  ORF Nucleotide Sequence

  >MR227128 ORF sequence
  Red=Cloning site Blue=ORF Green=Tags(s)
  
  TTTTGTAATACGACTCACTATAGGGCGGCCGGGAATTCGTCGACTGGATCCGGTACCGAGGAGATCTGCC
  GCCGCGATCGCC
  
  ATGACAGAAGAAGTCATTGTCATAGCCAAGTGGGACTACACTGCCCAGCAGGACCAGGAGCTCGACATCA
  GGAAGAACGAGCGGTTGTGGCTGCTAGATGACTCCAAGACGTGGTGGCGGGTGAGGAATGCTGCCAACAG
  GACAGGCTACGTGCCTTCCAACTATGTGGAGCGTAAGAACAGCCTGAAGAAGGGCTCCCTGGTGAAGAAC
  CTCAAGGACACACTAGGCCTCGGAAAGACGCGCAGGAAGCCAAGCGCTCGGGATGCTTCCCCAACGCCAA
  GCACTGATGCGGAGTACCCAGCCAATGGCAGCGGTGCTGACCGCATCTATGACCTCAACATCCCAGCCTT
  TGTCAAGTTCGCCTACGTAGCAGAACGAGAGGATGAGCTGTCCCTGGTGAAGGGCTCCCGTGTCACAGTT
  ATGGAGAAATGCAGTGATGGCTGGTGGCGTGGCAGCTTCAACGGGCAGATCGGCTGGTTCCCCTCCAACT
  ACGTTTTGGAGGAAGCGGACGAAGCTGCTGCTGAGGCCCCCAGCTTCCTGAGCCTACGTCGGGGCACTGC
  ACTGAGCAATGGGCAGGGTGCACGGGTGCTGCACGTGGTGCAGACGCTGTACCCCTTCAGCTCGGTCACT
  GAGGAGGAGCTTAGTTTCGAGAAGGGGGAGACCATGGAGGTGATTGAGAAGCCGGAGAATGACCCTGAAT
  GGTGGAAATGCAAAAATGCCCGAGGCCAAGTGGGCCTGGTCCCCAAAAACTACGTGGTTGTTCTCAGTGA
  TGGGCCTGCTCTGCACCCCGCTCACACCCCCCAGATCAGCTACACCGGGCCTTCAGCCAGCGGGCGCTTT
  GCTGGTCGGGAGTGGTACTATGGCAACGTGACACGGCACCAGGCCGAGTGTGCGCTCAATGAGCGGGGCG
  TCGAGGGCGACTTCCTCATTAGGGACAGCGAGTCCTCGCCCAGTGACTTCTCCGTGTCTCTCAAAGCGTC
  AGGGAGAAACAAGCACTTCAAGGTGCAGCTGGTGGACAGCGTCTACTGCATTGGGCAGCGGCGGTTCCAC
  AGCATGGACGAGCTTGTGGAGCACTACAAGAAGGCCCCCATCTTCACCAGCGAGCACGGGGAGAAGCTCT
  ACCTTGTCCGAGCCCTACAG
  
  ACGCGTACGCGGCCGCTCGAGCAGAAACTCATCTCAGAAGAGGATCTGGCAGCAAATGATATCCTGGATT
  ACAAGGATGACGACGATAAGGTTTAA
  Protein Sequence

  >MR227128 protein sequence
  Red=Cloning site Green=Tags(s)
  
  MTEEVIVIAKWDYTAQQDQELDIRKNERLWLLDDSKTWWRVRNAANRTGYVPSNYVERKNSLKKGSLVKN
  LKDTLGLGKTRRKPSARDASPTPSTDAEYPANGSGADRIYDLNIPAFVKFAYVAEREDELSLVKGSRVTV
  MEKCSDGWWRGSFNGQIGWFPSNYVLEEADEAAAEAPSFLSLRRGTALSNGQGARVLHVVQTLYPFSSVT
  EEELSFEKGETMEVIEKPENDPEWWKCKNARGQVGLVPKNYVVVLSDGPALHPAHTPQISYTGPSASGRF
  AGREWYYGNVTRHQAECALNERGVEGDFLIRDSESSPSDFSVSLKASGRNKHFKVQLVDSVYCIGQRRFH
  SMDELVEHYKKAPIFTSEHGEKLYLVRALQ
  
  TRTRPLEQKLISEEDLAANDILDYKDDDDKV
• Restriction Sites: SgfI-MluI Cloning Scheme for this gene Plasmid Map
• ACCN: NM_010879
• ORF Size: 1143 bp
• OTI Disclaimer: The molecular sequence of this clone aligns with the gene accession number as a point of reference only. However, individual transcript sequences of the same gene can differ through naturally occurring variations (e.g. polymorphisms), each with its own valid existence. This clone is substantially in agreement with the reference, but a complete review of all prevailing variants is recommended prior to use. More info
• OTI Annotation: This clone was engineered to express the complete ORF with an expression tag. Expression varies depending on the nature of the gene.

Reference Data

• RefSeq: NM_010879.1, NM_010879.2, NM_010879.3, NP_035009.3
• RefSeq Size: 2789
• RefSeq ORF: 1143
• Locus ID: 17974
• MW: 42.9 kDa