Pik3cg (NM_001146200) Mouse Tagged ORF Clone

CAT#: MR227194

Pik3cg (Myc-DDK-tagged) - Mouse phosphoinositide-3-kinase, catalytic, gamma polypeptide (Pik3cg), transcript variant 3

Specifications

Product Data

• Type: Mouse Tagged ORF Clone
• Tag: Myc-DDK
• Symbol: Pik3cg
• Synonyms: 5830428L06Rik; p110gamma; p120-PI3K; PI3Kgamma
• Vector: pCMV6-Entry
• E. coli Selection: Kanamycin (25 ug/mL)
• Mammalian Cell Selection: Neomycin
• Sequence Data:
  ORF Nucleotide Sequence

  >MR227194 representing NM_001146200
  Red=Cloning site Blue=ORF Green=Tags(s)
  
  TTTTGTAATACGACTCACTATAGGGCGGCCGGGAATTCGTCGACTGGATCCGGTACCGAGGAGATCTGCC
  GCCGCGATCGCC
  
  ATGGAGCTGGAGAACTATGAACAACCGGTGGTTCTAAGAGAGGACAACCTCCGCCGGCGCCGGAGGATGA
  AGCCACGCAGCGCAGCAGGCAGCCTGTCTTCCATGGAGCTCATCCCCATTGAGTTCGTACTGCCCACCAG
  CCAGCGCATCAGCAAGACTCCAGAAACAGCGCTGCTGCATGTGGCTGGCCATGGCAATGTGGAACAGATG
  AAAGCTCAGGTGTGGCTGCGCGCACTGGAGACCAGTGTGGCTGCGGAGTTCTACCACCGATTGGGCCCGG
  ACCAATTCCTCCTGCTCTACCAGAAGAAAGGACAATGGTATGAGATCTATGACAGGTACCAAGTGGTGCA
  GACCCTAGACTGCCTGCATTACTGGAAGTTGATGCACAAGAGCCCTGGCCAGATCCACGTGGTACAGCGA
  CACGTACCTTCTGAGGAGACCTTGGCTTTCCAGAAGCAGCTCACCTCCCTGATTGGCTATGACGTCACTG
  ACATCAGCAATGTGCACGATGATGAGCTAGAGTTCACTCGCCGCCGTCTGGTTACGCCCCGCATGGCTGA
  AGTGGCTGGCCGGGATGCCAAACTCTATGCTATGCACCCTTGGGTAACGTCCAAACCTCTCCCAGACTAC
  CTGTCAAAAAAGATTGCCAACAACTGCATCTTCATCGTCATCCACCGCGGTACCACCAGCCAAACCATCA
  AGGTCTCCGCAGATGATACTCCTGGTACCATCCTCCAGAGCTTCTTCACCAAGATGGCCAAGAAGAAGTC
  CCTAATGAATATCTCAGAAAGTCAAAGTGAGCAGGATTTTGTATTGCGGGTTTGTGGCCGCGATGAGTAC
  CTGGTGGGTGAAACACCCCTCAAAAATTTCCAGTGGGTGAGGCAGTGCCTCAAGAACGGAGATGAAATAC
  ACCTGGTGCTCGACACGCCTCCAGACCCAGCCCTTGATGAGGTGAGGAAGGAAGAATGGCCGCTGGTGGA
  TGACTGCACTGGAGTCACCGGCTACCACGAGCAGCTGACCATCCATGGCAAGGACCACGAGAGTGTGTTC
  ACAGTGTCTTTGTGGGACTGCGACCGAAAGTTCAGGGTCAAGATCAGAGGCATTGATATCCCTGTCCTGC
  CTCGGAACACCGACCTCACTGTGTTTGTGGAAGCGAACATCCAGCACGGGCAACAAGTCCTCTGCCAAAG
  GAGAACCAGCCCTAAGCCCTTCGCAGAAGAGGTACTCTGGAATGTGTGGCTGGAGTTTGGCATCAAAATC
  AAAGACTTGCCCAAAGGGGCTCTATTGAACCTACAGATCTACTGCTGCAAAACCCCATCACTGTCCAGCA
  AGGCTTCTGCAGAGACTCCAGGCTCCGAGTCCAAGGGCAAAGCCCAGCTTCTCTATTACGTGAACTTGCT
  GTTAATAGACCACCGTTTCCTCCTCCGCCACGGGGACTATGTGCTCCACATGTGGCAGATATCTGGCAAG
  GCAGAGGAGCAGGGCAGCTTCAATGCTGACAAGCTCACATCCGCAACCAATCCTGACAAGGAGAACTCAA
  TGTCCATTTCCATCCTGCTGGACAATTACTGTCACCCCATAGCTTTGCCTAAGCACCGGCCCACCCCTGA
  CCCAGAGGGAGACAGGGTTCGGGCTGAAATGCCCAATCAGCTTCGAAAGCAATTGGAGGCGATCATAGCC
  ACAGATCCACTTAACCCCCTCACAGCAGAGGACAAAGAATTGCTCTGGCATTTTCGATATGAAAGCCTGA
  AGCATCCGAAGGCTTACCCTAAGCTATTCAGCTCAGTGAAATGGGGGCAGCAAGAAATTGTTGCCAAAAC
  GTACCAGCTGTTAGCCAGAAGGGAGATCTGGGATCAAAGTGCTTTGGACGTTGGCTTAACCATGCAGCTC
  CTGGACTGCAACTTTTCAGACGAGAATGTCCGGGCCATTGCAGTTCAGAAACTGGAGAGCTTAGAGGACG
  ATGACGTTTTACATTACCTTCTCCAGCTGGTACAGGCTGTGAAATTTGAACCGTACCACGACAGTGCGCT
  GGCCAGATTCCTGCTGAAGCGTGGCTTGAGGAACAAAAGAATCGGTCACTTCTTGTTCTGGTTCCTGCGA
  AGTGAGATCGCACAGTCCAGACACTATCAGCAGAGGTTCGCTGTGATCCTGGAGGCGTACCTGCGAGGCT
  GTGGCACAGCCATGTTGCAGGACTTCACACAGCAGGTCCATGTGATTGAGATGTTACAGAAAGTCACCAT
  TGATATTAAATCGCTCTCGGCAGAGAAGTATGACGTCAGTTCCCAAGTTATTTCACAGCTTAAGCAAAAG
  CTTGAAAGCCTTCAGAACTCCAATCTCCCCGAGAGCTTTAGAGTTCCCTATGATCCTGGACTAAAAGCCG
  GTACCCTGGTGATCGAGAAATGCAAAGTGATGGCCTCCAAGAAGAAGCCCCTGTGGCTTGAGTTTAAGTG
  TGCTGATCCCACAGTCCTATCCAACGAAACCATTGGAATCATCTTTAAACATGGTGATGATCTGCGCCAA
  GACATGTTGATCTTGCAGATTCTACGCATCATGGAGTCCATTTGGGAGACTGAATCTCTGGACCTGTGCC
  TTCTGCCTTACGGTTGCATCTCAACTGGTGACAAAATAGGAATGATCGAGATTGTAAAGGATGCCACAAC
  GATCGCTCAAATTCAGCAAAGCACAGTGGGTAACACGGGGGCATTCAAAGATGAAGTCCTGAATCACTGG
  CTCAAGGAAAAATGTCCTATTGAAGAAAAGTTTCAGGCCGCAGTGGAAAGGTTTGTTTACTCCTGTGCAG
  GCTACTGTGTGGCCACATTTGTTCTTGGGATCGGTGACAGGCACAACGACAACATTATGATCTCAGAGAC
  AGGAAACCTATTTCATATAGACTTCGGACACATTCTTGGGAATTACAAGAGTTTCCTGGGCATCAATAAA
  GAGAGAGTGCCCTTCGTCCTAACCCCAGACTTCTTGTTTGTGATGGGATCTTCTGGAAAAAAGACAAGTC
  CACACTTCCAGAAATTCCAGGATGTCTGTGTTAGAGCTTACCTAGCTCTTCGCCATCACACAAACCTGTT
  GATCATCTTGTTCTCCATGATGCTGATGACAGGAATGCCCCAGCTGACAAGCAAAGAGGACATTGAATAT
  ATCCGGGATGCCCTCACCGTGGGAAAAAGCGAGGAGGACGCTAAGAAATATTTCCTTGATCAGATCGAAG
  TCTGCAGAGACAAAGGATGGACTGTGCAGTTTAACTGGTTCCTACATCTTGTTCTTGGCATCAAACAAGG
  AGAAAAGCACTCCGCT
  
  ACGCGTACGCGGCCGCTCGAGCAGAAACTCATCTCAGAAGAGGATCTGGCAGCAAATGATATCCTGGATT
  ACAAGGATGACGACGATAAGGTTTAA
  Protein Sequence

  >MR227194 representing NM_001146200
  Red=Cloning site Green=Tags(s)
  
  MELENYEQPVVLREDNLRRRRRMKPRSAAGSLSSMELIPIEFVLPTSQRISKTPETALLHVAGHGNVEQM
  KAQVWLRALETSVAAEFYHRLGPDQFLLLYQKKGQWYEIYDRYQVVQTLDCLHYWKLMHKSPGQIHVVQR
  HVPSEETLAFQKQLTSLIGYDVTDISNVHDDELEFTRRRLVTPRMAEVAGRDAKLYAMHPWVTSKPLPDY
  LSKKIANNCIFIVIHRGTTSQTIKVSADDTPGTILQSFFTKMAKKKSLMNISESQSEQDFVLRVCGRDEY
  LVGETPLKNFQWVRQCLKNGDEIHLVLDTPPDPALDEVRKEEWPLVDDCTGVTGYHEQLTIHGKDHESVF
  TVSLWDCDRKFRVKIRGIDIPVLPRNTDLTVFVEANIQHGQQVLCQRRTSPKPFAEEVLWNVWLEFGIKI
  KDLPKGALLNLQIYCCKTPSLSSKASAETPGSESKGKAQLLYYVNLLLIDHRFLLRHGDYVLHMWQISGK
  AEEQGSFNADKLTSATNPDKENSMSISILLDNYCHPIALPKHRPTPDPEGDRVRAEMPNQLRKQLEAIIA
  TDPLNPLTAEDKELLWHFRYESLKHPKAYPKLFSSVKWGQQEIVAKTYQLLARREIWDQSALDVGLTMQL
  LDCNFSDENVRAIAVQKLESLEDDDVLHYLLQLVQAVKFEPYHDSALARFLLKRGLRNKRIGHFLFWFLR
  SEIAQSRHYQQRFAVILEAYLRGCGTAMLQDFTQQVHVIEMLQKVTIDIKSLSAEKYDVSSQVISQLKQK
  LESLQNSNLPESFRVPYDPGLKAGTLVIEKCKVMASKKKPLWLEFKCADPTVLSNETIGIIFKHGDDLRQ
  DMLILQILRIMESIWETESLDLCLLPYGCISTGDKIGMIEIVKDATTIAQIQQSTVGNTGAFKDEVLNHW
  LKEKCPIEEKFQAAVERFVYSCAGYCVATFVLGIGDRHNDNIMISETGNLFHIDFGHILGNYKSFLGINK
  ERVPFVLTPDFLFVMGSSGKKTSPHFQKFQDVCVRAYLALRHHTNLLIILFSMMLMTGMPQLTSKEDIEY
  IRDALTVGKSEEDAKKYFLDQIEVCRDKGWTVQFNWFLHLVLGIKQGEKHSA
  
  TRTRPLEQKLISEEDLAANDILDYKDDDDKV
• Restriction Sites: SgfI-MluI Cloning Scheme for this gene Plasmid Map
• ACCN: NM_001146200
• ORF Size: 3306 bp
• OTI Disclaimer: The molecular sequence of this clone aligns with the gene accession number as a point of reference only. However, individual transcript sequences of the same gene can differ through naturally occurring variations (e.g. polymorphisms), each with its own valid existence. This clone is substantially in agreement with the reference, but a complete review of all prevailing variants is recommended prior to use. More info
• OTI Annotation: This clone was engineered to express the complete ORF with an expression tag. Expression varies depending on the nature of the gene.

Reference Data

• RefSeq: NM_001146200.1, NP_001139672.1
• RefSeq Size: 6634
• RefSeq ORF: 3309
• Locus ID: 30955
• MW: 126.4 kDa