Pdpk1 (NM_001080773) Mouse Tagged ORF Clone

CAT#: MR227245

Pdpk1 (Myc-DDK-tagged) - Mouse 3-phosphoinositide dependent protein kinase 1 (Pdpk1), transcript variant 2

Specifications

Product Data

• Type: Mouse Tagged ORF Clone
• Tag: Myc-DDK
• Symbol: Pdpk1
• Synonyms: Pdk1
• Vector: pCMV6-Entry
• E. coli Selection: Kanamycin (25 ug/mL)
• Mammalian Cell Selection: Neomycin
• Sequence Data:
  ORF Nucleotide Sequence

  >MR227245 representing NM_001080773
  Red=Cloning site Blue=ORF Green=Tags(s)
  
  TTTTGTAATACGACTCACTATAGGGCGGCCGGGAATTCGTCGACTGGATCCGGTACCGAGGAGATCTGCC
  GCCGCGATCGCC
  
  ATGGCCAGGACCACCAGCCAGCTGTATGACGCTGTGCCCATTCAGTCCAGTGTGGTGCTATGTTCCTGCC
  CATCCCCATCAATGGTGAGGTCCCAGACTGAGCCCGGTTCGTCCCCTGGCATTCCTAGTGGTGTTAGCAG
  GCAGGGATCCACCATGGATGGCACCACAGCTGAAGCCCGACCAAGCACCAACCCCTTGCAGCAGCACCCT
  GCCCAGCTGCCACCACAGCCTCGCAAGAAACGCCCTGAAGACTTCAAGTTTGGGAAAATTCTTGGCGAGG
  GCTCTTTTTCAACAGTTGTTCTGGCCCGAGAACTGGCCACTTCCAGAGAATATGCTATTAAAATTCTGGA
  GAAACGTCATATTATAAAAGAAAACAAAGTTCCGTATGTAACTAGAGAGAGAGATGTGATGTCACGCCTG
  GATCACCCCTTCTTTGTGAAACTTTATTTTACATTTCAGGACGACGAAAAGCTGTATTTTGGCCTTAGTT
  ATGCCAAAAATGGAGAGCTACTTAAATACATCCGCAAAATTGGCTCATTTGATGAGACCTGTACCCGGTT
  TTACACGGCTGAGATTGTGTCTGCTTTAGAGTACTTGCATGGCAAGGGCATCATTCACAGAGACCTTAAA
  CCAGAAAACATTTTGTTAAATGAAGACATGCACATCCAGATCACAGATTTTGGAACAGCAAAAGTGTTAT
  CCCCAGAGAGCAAACAAGCCAGGGCCAACTCATTTGTAGGAACAGCACAGTATGTTTCTCCAGAGCTGCT
  CACAGAGAAGTCGGCGTGTAAAAGTTCAGACCTTTGGGCCCTTGGATGTATAATCTATCAGCTCGTGGCA
  GGACTCCCACCATTCAGAGCCGGGAATGAATATCTTATATTTCAGAAGATCATTAAGCTGGAATATCATT
  TCCCAGAAAAATTCTTCCCTAAGGCTAGAGATCTTGTGGAAAAACTCTTGGTTTTAGATGCCACAAAGCG
  TTTAGGCTGTGAAGAGATGGAAGGGTACGGGCCTCTCAAAGCTCATCCATTCTTTGAGACCATCACTTGG
  GAGAATTTGCACCAGCAGACACCTCCGAAGCTCACAGCTTACCTACCAGCCATGTCAGAGGATGATGAAG
  ACTGCTATGGCAACTACGACAATCTCCTGAGCCAGTTTGGCTTCATGCAGGTGTCATCCTCCTCCTCTTC
  CCACTCCCTGTCTACGGTGGAAACCAGCCTGCCCCAGAGGTCGGGCAGCAACATAGAGCAGTACATCCAT
  GATTTGGACACTAACTCTTTTGAACTAGACTTACAGTTTTCAGAAGATGAAAAAAGGTTGTTATTGGAAA
  AGCAAGCCGGTGGAAACCCTTGGCACCAGTTTGTAGAAAATAATCTAATATTAAAAATGGGTCCAGTGGA
  TAAGCGAAAGGGTTTATTTGCAAGACGACGACAGTTATTACTCACAGAAGGGCCACATTTATATTATGTT
  GATCCTGTCAACAAGGTCTTGAAAGGTGAAATCCCATGGTCACAAGAACTCCGACCAGAAGCCAAGAATT
  TTAAAACTTTCTTTGTCCACACGATG
  
  ACGCGTACGCGGCCGCTCGAGCAGAAACTCATCTCAGAAGAGGATCTGGCAGCAAATGATATCCTGGATT
  ACAAGGATGACGACGATAAGGTTTAA
  Protein Sequence

  >MR227245 representing NM_001080773
  Red=Cloning site Green=Tags(s)
  
  MARTTSQLYDAVPIQSSVVLCSCPSPSMVRSQTEPGSSPGIPSGVSRQGSTMDGTTAEARPSTNPLQQHP
  AQLPPQPRKKRPEDFKFGKILGEGSFSTVVLARELATSREYAIKILEKRHIIKENKVPYVTRERDVMSRL
  DHPFFVKLYFTFQDDEKLYFGLSYAKNGELLKYIRKIGSFDETCTRFYTAEIVSALEYLHGKGIIHRDLK
  PENILLNEDMHIQITDFGTAKVLSPESKQARANSFVGTAQYVSPELLTEKSACKSSDLWALGCIIYQLVA
  GLPPFRAGNEYLIFQKIIKLEYHFPEKFFPKARDLVEKLLVLDATKRLGCEEMEGYGPLKAHPFFETITW
  ENLHQQTPPKLTAYLPAMSEDDEDCYGNYDNLLSQFGFMQVSSSSSSHSLSTVETSLPQRSGSNIEQYIH
  DLDTNSFELDLQFSEDEKRLLLEKQAGGNPWHQFVENNLILKMGPVDKRKGLFARRRQLLLTEGPHLYYV
  DPVNKVLKGEIPWSQELRPEAKNFKTFFVHTM
  
  TRTRPLEQKLISEEDLAANDILDYKDDDDKV
• Restriction Sites: SgfI-MluI Cloning Scheme for this gene Plasmid Map
• ACCN: NM_001080773
• ORF Size: 1566 bp
• OTI Disclaimer: The molecular sequence of this clone aligns with the gene accession number as a point of reference only. However, individual transcript sequences of the same gene can differ through naturally occurring variations (e.g. polymorphisms), each with its own valid existence. This clone is substantially in agreement with the reference, but a complete review of all prevailing variants is recommended prior to use. More info
• OTI Annotation: This clone was engineered to express the complete ORF with an expression tag. Expression varies depending on the nature of the gene.

Reference Data

• RefSeq: NM_001080773.1, NM_001080773.2, NP_001074242.1
• RefSeq Size: 1778
• RefSeq ORF: 1569
• Locus ID: 18607
• MW: 59.8 kDa