Psap (NM_011179) Mouse Tagged ORF Clone

CAT#: MR227313

Psap (Myc-DDK-tagged) - Mouse prosaposin (Psap), transcript variant 2

Specifications

Product Data

• Type: Mouse Tagged ORF Clone
• Tag: Myc-DDK
• Symbol: Psap
• Synonyms: AI037048; SGP-1
• Vector: pCMV6-Entry
• E. coli Selection: Kanamycin (25 ug/mL)
• Mammalian Cell Selection: Neomycin
• Sequence Data:
  ORF Nucleotide Sequence

  >MR227313 representing NM_011179
  Red=Cloning site Blue=ORF Green=Tags(s)
  
  TTTTGTAATACGACTCACTATAGGGCGGCCGGGAATTCGTCGACTGGATCCGGTACCGAGGAGATCTGCC
  GCCGCGATCGCC
  
  ATGTACGCCCTCGCCCTCTTCGCCAGCCTTCTGGCCACCGCTCTGACCAGCCCTGTCCAAGACCCGAAGA
  CATGCTCTGGGGGCTCAGCAGTGCTGTGCAGAGATGTGAAGACGGCGGTGGACTGTGGGGCCGTGAAGCA
  CTGCCAGCAGATGGTCTGGAGCAAGCCCACAGCGAAATCCCTTCCTTGCGACATATGCAAAACTGTTGTC
  ACCGAAGCTGGGAACTTGCTGAAAGATAATGCTACGCAGGAGGAGATCCTTCATTACCTGGAGAAGACCT
  GTGAGTGGATTCATGACTCCAGCCTGTCGGCCTCGTGCAAGGAGGTGGTTGACTCTTACCTGCCTGTCAT
  CCTGGACATGATTAAGGGCGAGATGAGCAACCCTGGGGAAGTGTGCTCTGCGCTCAACCTCTGCCAGTCC
  CTTCAGGAGTACTTGGCCGAGCAAAACCAGAAACAGCTTGAGTCCAACAAGATCCCGGAGGTGGACATGG
  CCCGTGTGGTTGCCCCCTTCATGTCCAACATCCCTCTCCTGCTGTACCCTCAGGATCACCCCCGCAGCCA
  GCCCCAACCTAAGGCTAACGAGGACGTCTGCCAGGACTGTATGAAGCTGGTGTCTGATGTCCAGACTGCT
  GTGAAGACCAACTCCAGCTTTATCCAGGGCTTCGTGGACCACGTGAAGGAGGATTGTGACCGCTTGGGGC
  CAGGCGTGTCTGACATATGCAAGAACTACGTGGACCAGTATTCCGAGGTCTGTGTCCAGATGTTGATGCA
  CATGGATCAGCAACCCAAGGAAATCTGTGTGCTGGCTGGCTTCTGTAATGAGGTCAAGAGAGTGCCAATG
  AAGACTCTGGTCCCTGCCACCGAGACCATTAAGAACATCCTCCCTGCCCTGGAGATGATGGACCCCTATG
  AGCAGAATCTGGTCCAGGCCCACAATGTGATTTTATGCCAGACCTGTCAGTTTGTGATGAATAAGTTTTC
  TGAGCTGATTGTCAATAATGCCACTGAGGAGCTCCTAGTTAAAGGTTTGAGCAACGCATGCGCACTGCTC
  CCCGATCCTGCCAGAACCAAGTGCCAGGAGGTGGTGGGAACATTTGGCCCCTCCCTGTTGGACATCTTTA
  TCCATGAGGTAAACCCCAGCTCTCTGTGCGGTGTGATCGGCCTCTGTGCTGCCCGCCCGGAGTTGGTGGA
  GGCACTTGAGCAGCCTGCGCCAGCCATTGTATCTGCACTGCTCAAAGAGCCCACACCGCCAAAGCAGCCC
  GCACAGCCCAAGCAGTCGGCATTGCCCGCCCATGTGCCTCCTCAGAAGAATGGTGGGTTCTGTGAGGTGT
  GCAAGAAACTGGTCCTCTATTTGGAACATAACCTGGAGAAAAACAGCACCAAGGAGGAAATCCTGGCCGC
  ACTTGAGAAGGGCTGCAGCTTCCTGCCAGACCCTTACCAGAAGCAGTGCGATGACTTTGTGGCTGAGTAT
  GAGCCCTTGCTATTGGAGATCCTCGTGGAAGTGATGGATCCTGGATTTGTGTGCTCGAAAATTGGAGTTT
  GCCCTTCTGCCTATAAGCTGCTGCTGGGAACCGAGAAGTGTGTCTGGGGCCCTAGCTACTGGTGTCAGAA
  CATGGAGACTGCCGCCCGATGCAATGCTGTCGATCATTGCAAACGCCATGTGTGGAAC
  
  ACGCGTACGCGGCCGCTCGAGCAGAAACTCATCTCAGAAGAGGATCTGGCAGCAAATGATATCCTGGATT
  ACAAGGATGACGACGATAAGGTTTAA
  Protein Sequence

  >MR227313 representing NM_011179
  Red=Cloning site Green=Tags(s)
  
  MYALALFASLLATALTSPVQDPKTCSGGSAVLCRDVKTAVDCGAVKHCQQMVWSKPTAKSLPCDICKTVV
  TEAGNLLKDNATQEEILHYLEKTCEWIHDSSLSASCKEVVDSYLPVILDMIKGEMSNPGEVCSALNLCQS
  LQEYLAEQNQKQLESNKIPEVDMARVVAPFMSNIPLLLYPQDHPRSQPQPKANEDVCQDCMKLVSDVQTA
  VKTNSSFIQGFVDHVKEDCDRLGPGVSDICKNYVDQYSEVCVQMLMHMDQQPKEICVLAGFCNEVKRVPM
  KTLVPATETIKNILPALEMMDPYEQNLVQAHNVILCQTCQFVMNKFSELIVNNATEELLVKGLSNACALL
  PDPARTKCQEVVGTFGPSLLDIFIHEVNPSSLCGVIGLCAARPELVEALEQPAPAIVSALLKEPTPPKQP
  AQPKQSALPAHVPPQKNGGFCEVCKKLVLYLEHNLEKNSTKEEILAALEKGCSFLPDPYQKQCDDFVAEY
  EPLLLEILVEVMDPGFVCSKIGVCPSAYKLLLGTEKCVWGPSYWCQNMETAARCNAVDHCKRHVWN
  
  TRTRPLEQKLISEEDLAANDILDYKDDDDKV
• Restriction Sites: SgfI-MluI Cloning Scheme for this gene Plasmid Map
• ACCN: NM_011179
• ORF Size: 1668 bp
• OTI Disclaimer: Due to the inherent nature of this plasmid, standard methods to replicate additional amounts of DNA in E. coli are highly likely to result in mutations and/or rearrangements. Therefore, OriGene does not guarantee the capability to replicate this plasmid DNA. Additional amounts of DNA can be purchased from OriGene with batch-specific, full-sequence verification at a reduced cost. Please contact our customer care team at custsupport@origene.com or by calling 301.340.3188 option 3 for pricing and delivery.
  
  The molecular sequence of this clone aligns with the gene accession number as a point of reference only. However, individual transcript sequences of the same gene can differ through naturally occurring variations (e.g. polymorphisms), each with its own valid existence. This clone is substantially in agreement with the reference, but a complete review of all prevailing variants is recommended prior to use. More info
• OTI Annotation: This clone was engineered to express the complete ORF with an expression tag. Expression varies depending on the nature of the gene.

Reference Data

• RefSeq: NM_011179.1, NM_011179.2, NM_011179.3, NP_035309.3
• RefSeq Size: 2676
• RefSeq ORF: 1674
• Locus ID: 19156
• MW: 61.9 kDa
• Gene Summary: This gene encodes a multifunctional glycoprotein that plays a role in the intracellular metabolism of various sphingolipids or secreted into the plasma, milk or cerebrospinal fluid. The encoded protein undergoes proteolytic processing to generate four different polypeptides known as saposin A, B, C or D, that are required for the hydrolysis of certain sphingolipids by lysosomal hydrolases. Alternately, the encoded protein is secreted into body fluids where it exhibits neurotrophic and myelinotrophic activities. A complete lack of the encoded protein is fatal to mice either at the neonatal stage or within the first month due to severe leukodystrophy and sphingolipid accumulation. Alternative splicing results in multiple transcript variants encoding different isoforms that may undergo similar processing to generate the mature saposins. [provided by RefSeq, Sep 2015]