Kcnj6 (NM_001025590) Mouse Tagged ORF Clone

CAT#: MR227316

Kcnj6 (Myc-DDK-tagged) - Mouse potassium inwardly-rectifying channel, subfamily J, member 6 (Kcnj6), transcript variant Girk2C

Specifications

Product Data

• Type: Mouse Tagged ORF Clone
• Tag: Myc-DDK
• Symbol: Kcnj6
• Synonyms: BIR1; GIRK2; KATP2; KCNJ7; Kir3.2; weaver; wv
• Vector: pCMV6-Entry
• E. coli Selection: Kanamycin (25 ug/mL)
• Mammalian Cell Selection: Neomycin
• Sequence Data:
  ORF Nucleotide Sequence

  >MR227316 representing NM_001025590
  Red=Cloning site Blue=ORF Green=Tags(s)
  
  TTTTGTAATACGACTCACTATAGGGCGGCCGGGAATTCGTCGACTGGATCCGGTACCGAGGAGATCTGCC
  GCCGCGATCGCC
  
  ATGGACCAGGATGTGGAAAGCCCAGTGGCCATTCACCAGCCAAAGTTGCCTAAGCAGGCCAGGGACGACC
  TGCCGAGACACATCAGCCGAGACAGGACCAAAAGGAAAATCCAGAGGTACGTGAGGAAGGATGGGAAGTG
  CAACGTTCACCACGGCAATGTGCGGGAGACGTACCGATACCTGACGGACATCTTCACCACCCTGGTGGAC
  CTGAAGTGGAGATTCAACCTGTTGATCTTTGTCATGGTCTACACAGTGACGTGGCTTTTCTTTGGGATGA
  TCTGGTGGCTGATTGCGTACATCCGGGGAGATATGGACCACATAGAGGACCCCTCGTGGACTCCTTGTGT
  CACCAACCTCAACGGGTTTGTCTCTGCTTTTTTATTCTCCATAGAGACAGAAACCACCATCGGTTATGGC
  TACCGGGTCATCACGGACAAGTGCCCTGAGGGGATTATTCTCCTCTTAATCCAGTCCGTGTTGGGGTCCA
  TTGTCAACGCCTTCATGGTAGGATGTATGTTTGTGAAAATATCCCAACCCAAGAAGAGGGCAGAGACCCT
  GGTCTTTTCCACCCACGCGGTGATCTCCATGCGGGATGGGAAACTGTGCTTGATGTTCCGGGTGGGGGAC
  TTGAGGAATTCTCACATTGTGGAGGCATCCATCAGAGCCAAGTTGATCAAGTCCAAACAGACTTCAGAGG
  GGGAGTTTATTCCCCTCAACCAGACTGATATCAACGTGGGGTACTACACAGGGGACGACCGGCTCTTTCT
  GGTGTCACCATTGATTATTAGCCATGAAATTAACCAACAGAGTCCCTTCTGGGAGATCTCCAAAGCGCAG
  CTGCCTAAAGAGGAACTGGAGATTGTGGTCATCCTGGAGGGAATGGTGGAAGCCACAGGGCAGTTC
  
  ACGCGTACGCGGCCGCTCGAGCAGAAACTCATCTCAGAAGAGGATCTGGCAGCAAATGATATCCTGGATT
  ACAAGGATGACGACGATAAGGTTTAA
  Protein Sequence

  >MR227316 representing NM_001025590
  Red=Cloning site Green=Tags(s)
  
  MDQDVESPVAIHQPKLPKQARDDLPRHISRDRTKRKIQRYVRKDGKCNVHHGNVRETYRYLTDIFTTLVD
  LKWRFNLLIFVMVYTVTWLFFGMIWWLIAYIRGDMDHIEDPSWTPCVTNLNGFVSAFLFSIETETTIGYG
  YRVITDKCPEGIILLLIQSVLGSIVNAFMVGCMFVKISQPKKRAETLVFSTHAVISMRDGKLCLMFRVGD
  LRNSHIVEASIRAKLIKSKQTSEGEFIPLNQTDINVGYYTGDDRLFLVSPLIISHEINQQSPFWEISKAQ
  LPKEELEIVVILEGMVEATGQF
  
  TRTRPLEQKLISEEDLAANDILDYKDDDDKV
• Restriction Sites: SgfI-MluI Cloning Scheme for this gene Plasmid Map
• ACCN: NM_001025590
• ORF Size: 906 bp
• OTI Disclaimer: The molecular sequence of this clone aligns with the gene accession number as a point of reference only. However, individual transcript sequences of the same gene can differ through naturally occurring variations (e.g. polymorphisms), each with its own valid existence. This clone is substantially in agreement with the reference, but a complete review of all prevailing variants is recommended prior to use. More info
• OTI Annotation: This clone was engineered to express the complete ORF with an expression tag. Expression varies depending on the nature of the gene.
• Product Components: The ORF clone is ion-exchange column purified, transfection-ready dried plasmid DNA, and shipped with 2 vector sequencing primers.
• Reconstitution: 1. Centrifuge at 5,000xg for 5min.
  2. Carefully open the tube and add 100ul of sterile water to dissolve the DNA.
  3. Close the tube and incubate for 10 minutes at room temperature.
  4. Briefly vortex the tube and then do a quick spin (less than 5000xg) to concentrate the liquid at the bottom.
  5. Store the suspended plasmid at -20°C. The DNA is stable for at least one year from date of shipping when stored at -20°C.

Reference Data

• RefSeq: NM_001025590.1, NP_001020761.1
• RefSeq Size: 3638 bp
• RefSeq ORF: 909 bp
• Locus ID: 16522
• Cytogenetics: 16 55.44 cM
• MW: 35.2 kDa
• Gene Summary: This potassium channel is controlled by G proteins. It plays a role in granule cell differentiation, possibly via membrane hyperpolarization. Inward rectifier potassium channels are characterized by a greater tendency to allow potassium to flow into the cell rather than out of it. Their voltage dependence is regulated by the concentration of extracellular potassium; as external potassium is raised, the voltage range of the channel opening shifts to more positive voltages. The inward rectification is mainly due to the blockage of outward current by internal magnesium. [UniProtKB/Swiss-Prot Function]