Psap (NM_001146123) Mouse Tagged ORF Clone

CAT#: MR227327

Psap (Myc-DDK-tagged) - Mouse prosaposin (Psap), transcript variant 5

Specifications

Product Data

• Type: Mouse Tagged ORF Clone
• Tag: Myc-DDK
• Symbol: Psap
• Synonyms: AI037048; SGP-1
• Vector: pCMV6-Entry
• E. coli Selection: Kanamycin (25 ug/mL)
• Mammalian Cell Selection: Neomycin
• Sequence Data:
  ORF Nucleotide Sequence

  >MR227327 representing NM_001146123
  Red=Cloning site Blue=ORF Green=Tags(s)
  
  TTTTGTAATACGACTCACTATAGGGCGGCCGGGAATTCGTCGACTGGATCCGGTACCGAGGAGATCTGCC
  GCCGCGATCGCC
  
  ATGTACGCCCTCGCCCTCTTCGCCAGCCTTCTGGCCACCGCTCTGACCAGCCCTGTCCAAGACCCGAAGA
  CATGCTCTGGGGGCTCAGCAGTGCTGTGCAGAGATGTGAAGACGGCGGTGGACTGTGGGGCCGTGAAGCA
  CTGCCAGCAGATGGTCTGGAGCAAGCCCACAGCGAAATCCCTTCCTTGCGACATATGCAAAACTGTTGTC
  ACCGAAGCTGGGAACTTGCTGAAAGATAATGCTACGCAGGAGGAGATCCTTCATTACCTGGAGAAGACCT
  GTGAGTGGATTCATGACTCCAGCCTGTCGGCCTCGTGCAAGGAGGTGGTTGACTCTTACCTGCCTGTCAT
  CCTGGACATGATTAAGGGCGAGATGAGCAACCCTGGGGAAGTGTGCTCTGCGCTCAACCTCTGCCAGTCC
  CTTCAGGAGTACTTGGCCGAGCAAAACCAGAAACAGCTTGAGTCCAACAAGATCCCGGAGGTGGACATGG
  CCCGTGTGGTTGCCCCCTTCATGTCCAACATCCCTCTCCTGCTGTACCCTCAGGATCACCCCCGCAGCCA
  GCCCCAACCTAAGGCTAACGAGGACGTCTGCCAGGACTGTATGAAGCTGGTGTCTGATGTCCAGACTGCT
  GTGAAGACCAACTCCAGCTTTATCCAGGGCTTCGTGGACCACGTGAAGGAGGATTGTGACCGCTTGGGGC
  CAGGCGTGTCTGACATATGCAAGAACTACGTGGACCAGTATTCCGAGCAACCCAAGGAAATCTGTGTGCT
  GGCTGGCTTCTGTAATGAGGTCAAGAGAGTGCCAATGAAGACTCTGGTCCCTGCCACCGAGACCATTAAG
  AACATCCTCCCTGCCCTGGAGATGATGGACCCCTATGAGCAGAATCTGGTCCAGGCCCACAATGTGATTT
  TATGCCAGACCTGTCAGTTTGTGATGAATAAGTTTTCTGAGCTGATTGTCAATAATGCCACTGAGGAGCT
  CCTAGTTAAAGGTTTGAGCAACGCATGCGCACTGCTCCCCGATCCTGCCAGAACCAAGTGCCAGGAGGTG
  GTGGGAACATTTGGCCCCTCCCTGTTGGACATCTTTATCCATGAGGTAAACCCCAGCTCTCTGTGCGGTG
  TGATCGGCCTCTGTGCTGCCCGCCCGGAGTTGGTGGAGGCACTTGAGCAGCCTGCGCCAGCCATTGTATC
  TGCACTGCTCAAAGAGCCCACACCGCCAAAGCAGCCCGCACAGCCCAAGCAGTCGGCATTGCCCGCCCAT
  GTGCCTCCTCAGAAGAATGGTGGGTTCTGTGAGGTGTGCAAGAAACTGGTCCTCTATTTGGAACATAACC
  TGGAGAAAAACAGCACCAAGGAGGAAATCCTGGCCGCACTTGAGAAGGGCTGCAGCTTCCTGCCAGACCC
  TTACCAGAAGCAGTGCGATGACTTTGTGGCTGAGTATGAGCCCTTGCTATTGGAGATCCTCGTGGAAGTG
  ATGGATCCTGGATTTGTGTGCTCGAAAATTGGAGTTTGCCCTTCTGCCTATAAGCTGCTGCTGGGAACCG
  AGAAGTGTGTCTGGGGCCCTAGCTACTGGTGTCAGAACATGGAGACTGCCGCCCGATGCAATGCTGTCGA
  TCATTGCAAACGCCATGTGTGGAAC
  
  ACGCGTACGCGGCCGCTCGAGCAGAAACTCATCTCAGAAGAGGATCTGGCAGCAAATGATATCCTGGATT
  ACAAGGATGACGACGATAAGGTTTAA
  Protein Sequence

  >MR227327 representing NM_001146123
  Red=Cloning site Green=Tags(s)
  
  MYALALFASLLATALTSPVQDPKTCSGGSAVLCRDVKTAVDCGAVKHCQQMVWSKPTAKSLPCDICKTVV
  TEAGNLLKDNATQEEILHYLEKTCEWIHDSSLSASCKEVVDSYLPVILDMIKGEMSNPGEVCSALNLCQS
  LQEYLAEQNQKQLESNKIPEVDMARVVAPFMSNIPLLLYPQDHPRSQPQPKANEDVCQDCMKLVSDVQTA
  VKTNSSFIQGFVDHVKEDCDRLGPGVSDICKNYVDQYSEQPKEICVLAGFCNEVKRVPMKTLVPATETIK
  NILPALEMMDPYEQNLVQAHNVILCQTCQFVMNKFSELIVNNATEELLVKGLSNACALLPDPARTKCQEV
  VGTFGPSLLDIFIHEVNPSSLCGVIGLCAARPELVEALEQPAPAIVSALLKEPTPPKQPAQPKQSALPAH
  VPPQKNGGFCEVCKKLVLYLEHNLEKNSTKEEILAALEKGCSFLPDPYQKQCDDFVAEYEPLLLEILVEV
  MDPGFVCSKIGVCPSAYKLLLGTEKCVWGPSYWCQNMETAARCNAVDHCKRHVWN
  
  TRTRPLEQKLISEEDLAANDILDYKDDDDKV
• Restriction Sites: SgfI-MluI Cloning Scheme for this gene Plasmid Map
• ACCN: NM_001146123
• ORF Size: 1635 bp
• OTI Disclaimer: The molecular sequence of this clone aligns with the gene accession number as a point of reference only. However, individual transcript sequences of the same gene can differ through naturally occurring variations (e.g. polymorphisms), each with its own valid existence. This clone is substantially in agreement with the reference, but a complete review of all prevailing variants is recommended prior to use. More info
• OTI Annotation: This clone was engineered to express the complete ORF with an expression tag. Expression varies depending on the nature of the gene.

Reference Data

• RefSeq: NM_001146123.1, NP_001139595.1
• RefSeq Size: 2640
• RefSeq ORF: 1638
• Locus ID: 19156
• MW: 60.4 kDa
• Gene Summary: This gene encodes a multifunctional glycoprotein that plays a role in the intracellular metabolism of various sphingolipids or secreted into the plasma, milk or cerebrospinal fluid. The encoded protein undergoes proteolytic processing to generate four different polypeptides known as saposin A, B, C or D, that are required for the hydrolysis of certain sphingolipids by lysosomal hydrolases. Alternately, the encoded protein is secreted into body fluids where it exhibits neurotrophic and myelinotrophic activities. A complete lack of the encoded protein is fatal to mice either at the neonatal stage or within the first month due to severe leukodystrophy and sphingolipid accumulation. Alternative splicing results in multiple transcript variants encoding different isoforms that may undergo similar processing to generate the mature saposins. [provided by RefSeq, Sep 2015]