Gucy2d (NM_001130693) Mouse Tagged ORF Clone

CAT#: MR227356

Gucy2d (Myc-DDK-tagged) - Mouse guanylate cyclase 2d (Gucy2d)

Specifications

Product Data

• Type: Mouse Tagged ORF Clone
• Tag: Myc-DDK
• Symbol: Gucy2d
• Vector: pCMV6-Entry
• E. coli Selection: Kanamycin (25 ug/mL)
• Mammalian Cell Selection: Neomycin
• Sequence Data:
  ORF Nucleotide Sequence

  >MR227356 representing NM_001130693
  Red=Cloning site Blue=ORF Green=Tags(s)
  
  TTTTGTAATACGACTCACTATAGGGCGGCCGGGAATTCGTCGACTGGATCCGGTACCGAGGAGATCTGCC
  GCCGCGATCGCC
  
  ATGGCAGGTCTGCAGCAGGGCTGTCACTTTGAAGGCCAGAACTGGACAGCACCACACTGGAAGACCTGCC
  TGCCCTGCCAGGGCCCGTGGAGACTCACTGTCAGTCATCTGAAGACTGTCTCTTCCATATCAGTCCTCTC
  TGTGGTATTTTGGAGTGTCCTACTTTGGGCTGACTCCCTTTCCCTGTTAGCATGGGCAAGAGAGACCTTC
  ACCCTTGGGGTGCTGGGTCCCTGGGACTGTGACCCCATCTTTGCCCAGGCCCTCCCCAGCATAGCTACCC
  AGCTGGCTGTAGATCAAGTTAATCAGGATGCCTCACTGCTGCCAGGCTCACAGTTGGATTTCAAGGTCCT
  CCCCACAGGCTGTGACACTCCTCATGCCCTGGCCACATTCGTGGCCCACAAGAACATAGTGGCTGCTTTT
  GTAGGCCCTGTTAATCCTGGGTTCTGCTCAGCAGCAGCCCTGCTGGCCCAAGGCTGGGGCAAGTCCCTCT
  TCTCCTGGGCCTGTGAAGCTCCAGAGGGAGGAGGTGACTTAGTGCCCACTTTACCTTCTGCGGCCGATGT
  GCTACTGTCTGTCATGAGACACTTTGGCTGGGCTCGCTGGGCCATTGTGTCCTCTCACCAGGACATCTGG
  GTAACCACAGCCCAGCAGCTGGCCACTGCTTTCAGGACTCATGGGCTGCCCATCGGACTGGTGACCTCTT
  TGGGACCTGGAGAGAAGGGGGCCACAGAGGTTTGCAAGCAGCTCCACAGTGTTCATGGTCTGAAAATTGT
  GGTTCTGTGCATGCACTCGGCGCTGCTTGGAGGCCTGGAGCAGACCACCCTGCTGCACTGTGCCTGGGAG
  GAGGGCCTCACAGACGGAAGGCTGGTCTTCCTGCCCTACGACACGCTGCTCTTTGCCCTGCCCTATGGAA
  ACCGCTCCTACCTGGTCCTGGATGACCATGGGCCCCTTCAGGAAGCCTATGACGCAGTACTCACTGTCAG
  TCTGGAGTCCAGTCCTGAGAGCCACGCCTTCACTGCTACCGAGATGAGTGGAGGGGCAACTGCCAACTTG
  GAGCCAGAGCAGGTGTCCCCACTCTTTGGAACTATCTATGATGCCGTCATCCTGTTGGCCCATGCCCTGA
  ACCGATCTGAGACCCATGGAGCGGGGCTCTCAGGGGCTCACTTAGGGGACCACGTAAGAGCTCTTGATGT
  GGCTGGTTTTAGCCAGAGAATCCGAACAGATGGGAAAGGCAGAAGGCTAGCCCAATATGTCATCCTGGAC
  ACAGATGGTGAAGGAAGCCAGTTGGTTCCTACCCACATTCTGGACACAAGCACATGGCAGGTGCAGCCTC
  TGGGAAAGCCTATACACTTTCCTGGGGGGAGCCCTCCAGCCCATGACGCCAGCTGCTGGTTCGACCCCAA
  CACACTATGCATCAGAGGTGTGCAGCCCCTGGGCAGCCTCCTGACTCTGACGATAGCCTGTGTCCTGGCA
  CTGGTTGGTGGATTCCTTGCTTACTTCATCAGGTTAGGCCTCCAGCAGCTGCGGCTGTTGCGGGGCCCCC
  ATCGGATCCTGCTGACATCTCAGGAGCTCACCTTCCTCCAGCGGACCCCCAGCCGGAGGAGGCCACATGT
  GGACAGCGGCAGTGAGTCAAGAAGTGTGGTGGATGGTGGAAGTCCACGGTCGGTGACCCAGGGGTCAGCA
  AGGAGCCTACCAGCCTTCCTGGAGCACACCAACGTGGCCCTGTACCAGGGAGAGTGGGTATGGCTGAAGA
  AGTTTGAAGCAGGCGTGGCTCCTGATCTTCGGCCAAGTAGCCTTAGCTTCCTGAGAAAGTTGCGGGAGAT
  GCGGCATGAGAATGTTACTGCCTTCCTGGGCCTCTTTGTGGGCCCTGGGGTCAGTGCAATGGTGCTGGAG
  CACTGTGCCCGTGGCAGCCTGGAGGACCTGCTGCAGAATGAGAACCTGAGGCTAGACTGGACCTTCAAGG
  CCTCCTTACTGTTGGATCTGATCCGAGGTTTGCGGTATCTGCACCATCGGCGCTTCCCCCACGGGCGCCT
  CAAGTCCAGGAACTGTGTGGTGGACACTCGCTTTGTGCTCAAGATCACTGATCATGGCTATGCAGAGTTC
  CTGGAGTCTCACTGTTCTTCCAGGCCCCAGCCAGCCCCAGAAGAGCTTCTGTGGACGGCTCCTGAGCTGC
  TGCGGGGGCCTGGGAAGGCCACCTTCAAAGGTGATGTTTTCAGCTTGGCCATCATCCTGCAGGAGGTACT
  GACCCGAGACCCACCCTACTGCTCCTGGGGACTCTCAGCAGAAGAGATCATCCGGAAGGTGGCATCTCCC
  CCTCCTCTGTGCCGGCCACTGGTGTCCCCTGACCAGGGTCCCCTCGAGTGTATCCAGTTGATGCAGCTAT
  GCTGGGAGGAAGCTCCAGATGACAGGCCAAGCTTGGACCAGATCTACACACAGTTCAAAAGCATCAACCA
  AGGCAAGAAGACAAGTGTTGTAGACTCCATGCTGCGGATGCTGGAGAAGTATTCCGAAAGCCTGGAAGAT
  CTGGTCCAGGAGCGGACTGAGGAGCTGGAACTGGAGAGGCGGAAGACAGAGAGGCTGCTCTCGCAGATGC
  TCCCTCCGTCTGTCGCCCATGCTCTAAAGATGGGGACAACAGTGGAGCCCGAGTACTTCGACCAGGTCAC
  CATCTATTTCAGTGACATCGTGGGCTTCACCACCATCTCAGCCTTGAGTGAGCCCATTGAGGTGGTGGGC
  TTCCTCAATGATCTCTACACGCTGTTTGATGCTGTTCTCGACAGCCATGATGTGTATAAGGTGGAGACCA
  TAGGGGATGCCTACATGGTGGCTTCTGGTCTCCCTCGGCGCAATGGAAATCGGCACGCTGCTGAGATCGC
  CAACCTGGCCCTGGACATCCTTAGCTATGCAGGCAACTTTCGGATGAGGCATGCACCCGATGTACCCATC
  CGTGTCAGGGCAGGTCTGCATTCAGGACCCTGTGTGGCAGGTGTTGTGGGTCTCACCATGCCTCGGTACT
  GCCTCTTTGGGGACACCGTCAACACTGCCTCTAGGATGGAGTCCACTGGGCTGCCTTACAGGATCCATGT
  CAGTCAAAGCACTGTCCAGGCCCTGCTCAGCCTTGATGAAGGCTACAAAATTGATGTCAGAGGTCAGACT
  GAACTGAAGGGGAAGGGCTTGGAGGAGACCTACTGGCTGACAGGGAAGGTAGGATTCTGCAGACCCCTGC
  CTACACCTCTGTCTATCAAGCCTGGAGACCCATGGCAGGACCGCATAAACCAAGAAATCCGGACTGGCTT
  TGCCAAAGCACGCCAGGGACTGGCTGAGCCCAGAAAATCAGGGGAGGCTGGGCCAGGTCCC
  
  ACGCGTACGCGGCCGCTCGAGCAGAAACTCATCTCAGAAGAGGATCTGGCAGCAAATGATATCCTGGATT
  ACAAGGATGACGACGATAAGGTTTAA
  Protein Sequence

  >MR227356 representing NM_001130693
  Red=Cloning site Green=Tags(s)
  
  MAGLQQGCHFEGQNWTAPHWKTCLPCQGPWRLTVSHLKTVSSISVLSVVFWSVLLWADSLSLLAWARETF
  TLGVLGPWDCDPIFAQALPSIATQLAVDQVNQDASLLPGSQLDFKVLPTGCDTPHALATFVAHKNIVAAF
  VGPVNPGFCSAAALLAQGWGKSLFSWACEAPEGGGDLVPTLPSAADVLLSVMRHFGWARWAIVSSHQDIW
  VTTAQQLATAFRTHGLPIGLVTSLGPGEKGATEVCKQLHSVHGLKIVVLCMHSALLGGLEQTTLLHCAWE
  EGLTDGRLVFLPYDTLLFALPYGNRSYLVLDDHGPLQEAYDAVLTVSLESSPESHAFTATEMSGGATANL
  EPEQVSPLFGTIYDAVILLAHALNRSETHGAGLSGAHLGDHVRALDVAGFSQRIRTDGKGRRLAQYVILD
  TDGEGSQLVPTHILDTSTWQVQPLGKPIHFPGGSPPAHDASCWFDPNTLCIRGVQPLGSLLTLTIACVLA
  LVGGFLAYFIRLGLQQLRLLRGPHRILLTSQELTFLQRTPSRRRPHVDSGSESRSVVDGGSPRSVTQGSA
  RSLPAFLEHTNVALYQGEWVWLKKFEAGVAPDLRPSSLSFLRKLREMRHENVTAFLGLFVGPGVSAMVLE
  HCARGSLEDLLQNENLRLDWTFKASLLLDLIRGLRYLHHRRFPHGRLKSRNCVVDTRFVLKITDHGYAEF
  LESHCSSRPQPAPEELLWTAPELLRGPGKATFKGDVFSLAIILQEVLTRDPPYCSWGLSAEEIIRKVASP
  PPLCRPLVSPDQGPLECIQLMQLCWEEAPDDRPSLDQIYTQFKSINQGKKTSVVDSMLRMLEKYSESLED
  LVQERTEELELERRKTERLLSQMLPPSVAHALKMGTTVEPEYFDQVTIYFSDIVGFTTISALSEPIEVVG
  FLNDLYTLFDAVLDSHDVYKVETIGDAYMVASGLPRRNGNRHAAEIANLALDILSYAGNFRMRHAPDVPI
  RVRAGLHSGPCVAGVVGLTMPRYCLFGDTVNTASRMESTGLPYRIHVSQSTVQALLSLDEGYKIDVRGQT
  ELKGKGLEETYWLTGKVGFCRPLPTPLSIKPGDPWQDRINQEIRTGFAKARQGLAEPRKSGEAGPGP
  
  TRTRPLEQKLISEEDLAANDILDYKDDDDKV
• Restriction Sites: SgfI-MluI Cloning Scheme for this gene Plasmid Map
• ACCN: NM_001130693
• ORF Size: 3351 bp
• OTI Disclaimer: The molecular sequence of this clone aligns with the gene accession number as a point of reference only. However, individual transcript sequences of the same gene can differ through naturally occurring variations (e.g. polymorphisms), each with its own valid existence. This clone is substantially in agreement with the reference, but a complete review of all prevailing variants is recommended prior to use. More info
• OTI Annotation: This clone was engineered to express the complete ORF with an expression tag. Expression varies depending on the nature of the gene.

Reference Data

• RefSeq: NM_001130693.1, NM_001130693.2, NM_001130693.3, NP_001124165.1
• RefSeq Size: 3633
• RefSeq ORF: 3354
• Locus ID: 14918
• MW: 122.5 kDa