Atp11c (NM_001001798) Mouse Tagged ORF Clone

CAT#: MR227364

Atp11c (Myc-DDK-tagged) - Mouse ATPase, class VI, type 11C (Atp11c), transcript variant 2

Specifications

Product Data

• Type: Mouse Tagged ORF Clone
• Tag: Myc-DDK
• Symbol: Atp11c
• Synonyms: A330005H02Rik; AI315324; Ig
• Vector: pCMV6-Entry
• E. coli Selection: Kanamycin (25 ug/mL)
• Mammalian Cell Selection: Neomycin
• Sequence Data:
  ORF Nucleotide Sequence

  >MR227364 representing NM_001001798
  Red=Cloning site Blue=ORF Green=Tags(s)
  
  TTTTGTAATACGACTCACTATAGGGCGGCCGGGAATTCGTCGACTGGATCCGGTACCGAGGAGATCTGCC
  GCCGCGATCGCC
  
  ATGTTCCGCCGGACCCTCAACCGTTTGTGTGCTGGAGAAGAGAAACGAGTTGGTACACGCACAGTGTTTG
  TTGGCAATCATCCCATTTCTGGAACAGAACCTTATATTGCGCAAAGATTTTGTGATAATAGAATAGTCTC
  ATCTAAGTATACACTTTGGAATTTCCTCCCTAAGAATTTGTTTGAACAGTTTAGAAGAATTGCGAATTTT
  TATTTCCTCATCATTTTCCTTGTACAGGTCACAGTAGACACACCAACCAGCCCAGTTACCAGTGGACTTC
  CACTTTTTTTCGTTATAACTGTTACAGCAATCAAGCAGGGGTATGAAGATTGGCTTAGACACAGAGCTGA
  TAATGAAGTTAACAAAAGTGCTGTTTATATTATTGAAAATGCAAAGCGAGTGAGGAAAGAAAGTGAAAAA
  ATCAAGGTTGGTGATGTAGTAGAAGTACAGGCAAATGAAACCTTTCCCTGTGATCTTATACTTCTGTCAT
  CCTGCACAACTGATGGAACCTGTTATGTCACTACAGCCAGTCTTGATGGTGAATCTAATTGCAAGACACA
  TTATGCAGTACGAGATACCATTGCACTGTGTACAGCCGAATCCATTGATAATCTCCGAGCAACAATTGAA
  TGTGAGCAGCCTCAACCTGATCTCTACAGGTTTGTTGGGCGAATCAGTATCTATAGTAATAGTATTGAGG
  CTGTTGCCAGGTCTTTGGGACCTGAAAATCTTTTGCTGAAAGGAGCCACACTTAAAAATACCAAGAAGAT
  ATATGGAGTTGCTGTTTACACTGGGATGGAAACCAAAATGGCTTTGAACTACCAAGGGAAATCTCAGAAA
  TGTTCTGCTGTTGAAAAATCTATTAATGCCTTCTTGATTGTTTATTTATTTATCTTACTGACCAAAGCTG
  CAGTATGCACAACTCTTAAGTATGTTTGGCAAAGTTCCCCATACAATGATGAACCATGGTATAACCAAAA
  GACTCAAAAGGAACGGGAAACTTTTCAGGTTTTGAAAATGTTCACTGACTTTTTATCATTCATGGTTCTT
  TTCAACTTCATTATACCTGTCTCCATGTATGTCACAGTAGAAATGCAGAAATTTTTAGGGTCATTCTTTA
  TTTCATGGGATAAAGACTTTTTTGATGAAGAAATTAATGAAGGAGCCTTGGTTAATACATCAGACCTTAA
  TGAAGAACTTGGTCAGGTGGACTATGTATTTACAGATAAGACTGGGACACTCACTGAAAATAGCATGGAA
  TTCATTGAATGCTGCATAGATGGGCACAAATATAAAGGCACAACTCAGGAAGTTGATGGATTATCTCAGA
  CTGATGGGCCCTTAGCCTATTTTGATAAAGCAGATAAGAACCGAGAGGCACTCTTTCTCCGTGCCCTATG
  CTTATGTCACACTGTAGAAATGAAAACAAATGATGATGTTGATGGACCTGTAGAAGGAGCCGGATTCACA
  TATATCTCCTCCTCACCAGATGAAATAGCTTTGGTGAAAGGAGCTAAAAGGTTTGGGTTCACATTTTTGG
  GAAATCAGAATGGATATATCAGAGTAGAGAACCAAAGAAAAGAAATAGAAGAGTATGAACTTCTCCACAC
  CTTAAATTTTGATTCTGTCCGCCGACGTATGAGTGTAATTGTAAGGACCCAAAAAGGAGATATTCTACTT
  TTCTGTAAAGGAGCAGATTCATCAATTTTTCCCAGGGTACATAGCCATCAAATTGAGTTAACCAAAGACC
  ATGTGGAACGTAATGCAATGGATGGGTATCGGACTCTTTGTGTAGCCTTCAAAGAAATTCCTCCAGATGA
  TTTTGAAAGAATCAATGCACAACTAGTAGAGGCAAAAATGGCCCTACAAGATAGAGAAGAAAAACTGGAA
  AAGGTTTTTGATGAGATTGAGACTAACATGAATTTAATTGGAGCCACTGCTGTGGAAGACAAGCTGCAAG
  ATCAGGCTGCAGAGACCATTGAAGCTCTCCATGCAGCTGGCTTAAAAGTCTGGGTGCTTACTGGGGACAA
  GATGGAAACAGCCAAATCTACTTGCTATGCCTGCCGCCTTTTCCAAACCAATACTGAGCTCTTGGAACTG
  ACCACAAAAACCATTGAAGAGAGTGAAAGGAAAGAAGATCGATTACATGAACTGCTAATAGAATATCGTA
  AGAAGTTGCTGCATGAATTTCCTAAAAGCACTAGAAGCCTTAAAAAAGCATGGACAGAACATCAGGAATA
  TGGATTAATCATTGATGGCTCCACATTGTCACTCATACTAAATTCTAGTCAAGATTGTAGTTCAAACAAC
  TATAAAAGTATTTTTCTACAAATCTGTATGAAATGCACTGCAGTGCTCTGCTGCCGGATGGCACCATTAC
  AAAAAGCCCAGATTGTCAGAATGGTGAAGAACTTGAAAGGCAGCCCCATAACACTGTCAATAGGTGATGG
  TGCCAATGATGTCAGTATGATTTTGGAATCCCATGTGGGAATAGGTATTAAAGGAAAAGAAGGCCGTCAA
  GCAGCCAGGAATAGTGATTATTCTGTTCCAAAGTTTAAGCATTTAAAGAAACTGCTATTGGTTCATGGAC
  ATCTATACTATGTGAGAATAGCACATCTTGTACAATATTTCTTCTACAAGAACCTTTGTTTCATTTTGCC
  ACAATTTTTGTACCAGTTCTTCTGTGGATTCTCACAACAGCCACTCTATGATGCTGCTTATCTTACAATG
  TACAATATCTGTTTCACATCCCTGCCCATCCTGGCTTATAGTCTACTGGAACAGCACATCAACATTGATA
  CTCTGACCGCAGACCCTCGATTGTATATGAAAATTACCGGTAATGCTATGTTACAGTTGGGGCCCTTCTT
  ACATTGGACATTTCTGGCTGCATTTGAAGGGACAGTATTCTTCTTTGGGACTTATTTCCTTTTTCAGACT
  TCATCCTTAGAAGACAATGGAAAGATTTATGGAAATTGGACATTTGGAACCATTGTTTTTACAGTCTTAG
  TATTCACCGTAACTCTGAAGCTCGCCTTGGATACCCGGTTCTGGACATGGATAAATCATTTTGTGATTTG
  GGGTTCTCTAGCCTTTTATGTTTTTTTCTCATTCTTCTGGGGAGGAATTATTTGGCCTTTTCTGAAACAA
  CAGAGAATGTATTTTGTGTTTGCTCAAATGCTCTGTTCTGTATCCACATGGTTGGCTATAATCCTTTTAA
  TATTTATCAGCCTTTTCCCTGAGATTCTCCTAATAGTTGTAAAGAATGTTCGAAGAAGAAGTGCCAGGAA
  TCCGAATCTTGAACTGCCTATGTTATTGTCCTACAAGCATATTGACCGTGGTTGCAGC
  
  ACGCGTACGCGGCCGCTCGAGCAGAAACTCATCTCAGAAGAGGATCTGGCAGCAAATGATATCCTGGATT
  ACAAGGATGACGACGATAAGGTTTAA
  Protein Sequence

  >MR227364 representing NM_001001798
  Red=Cloning site Green=Tags(s)
  
  MFRRTLNRLCAGEEKRVGTRTVFVGNHPISGTEPYIAQRFCDNRIVSSKYTLWNFLPKNLFEQFRRIANF
  YFLIIFLVQVTVDTPTSPVTSGLPLFFVITVTAIKQGYEDWLRHRADNEVNKSAVYIIENAKRVRKESEK
  IKVGDVVEVQANETFPCDLILLSSCTTDGTCYVTTASLDGESNCKTHYAVRDTIALCTAESIDNLRATIE
  CEQPQPDLYRFVGRISIYSNSIEAVARSLGPENLLLKGATLKNTKKIYGVAVYTGMETKMALNYQGKSQK
  CSAVEKSINAFLIVYLFILLTKAAVCTTLKYVWQSSPYNDEPWYNQKTQKERETFQVLKMFTDFLSFMVL
  FNFIIPVSMYVTVEMQKFLGSFFISWDKDFFDEEINEGALVNTSDLNEELGQVDYVFTDKTGTLTENSME
  FIECCIDGHKYKGTTQEVDGLSQTDGPLAYFDKADKNREALFLRALCLCHTVEMKTNDDVDGPVEGAGFT
  YISSSPDEIALVKGAKRFGFTFLGNQNGYIRVENQRKEIEEYELLHTLNFDSVRRRMSVIVRTQKGDILL
  FCKGADSSIFPRVHSHQIELTKDHVERNAMDGYRTLCVAFKEIPPDDFERINAQLVEAKMALQDREEKLE
  KVFDEIETNMNLIGATAVEDKLQDQAAETIEALHAAGLKVWVLTGDKMETAKSTCYACRLFQTNTELLEL
  TTKTIEESERKEDRLHELLIEYRKKLLHEFPKSTRSLKKAWTEHQEYGLIIDGSTLSLILNSSQDCSSNN
  YKSIFLQICMKCTAVLCCRMAPLQKAQIVRMVKNLKGSPITLSIGDGANDVSMILESHVGIGIKGKEGRQ
  AARNSDYSVPKFKHLKKLLLVHGHLYYVRIAHLVQYFFYKNLCFILPQFLYQFFCGFSQQPLYDAAYLTM
  YNICFTSLPILAYSLLEQHINIDTLTADPRLYMKITGNAMLQLGPFLHWTFLAAFEGTVFFFGTYFLFQT
  SSLEDNGKIYGNWTFGTIVFTVLVFTVTLKLALDTRFWTWINHFVIWGSLAFYVFFSFFWGGIIWPFLKQ
  QRMYFVFAQMLCSVSTWLAIILLIFISLFPEILLIVVKNVRRRSARNPNLELPMLLSYKHIDRGCS
  
  TRTRPLEQKLISEEDLAANDILDYKDDDDKV
• Restriction Sites: SgfI-MluI Cloning Scheme for this gene Plasmid Map
• ACCN: NM_001001798
• ORF Size: 3348 bp
• OTI Disclaimer: The molecular sequence of this clone aligns with the gene accession number as a point of reference only. However, individual transcript sequences of the same gene can differ through naturally occurring variations (e.g. polymorphisms), each with its own valid existence. This clone is substantially in agreement with the reference, but a complete review of all prevailing variants is recommended prior to use. More info
• OTI Annotation: This clone was engineered to express the complete ORF with an expression tag. Expression varies depending on the nature of the gene.
• Product Components: The ORF clone is ion-exchange column purified, transfection-ready dried plasmid DNA, and shipped with 2 vector sequencing primers.
• Reconstitution: 1. Centrifuge at 5,000xg for 5min.
  2. Carefully open the tube and add 100ul of sterile water to dissolve the DNA.
  3. Close the tube and incubate for 10 minutes at room temperature.
  4. Briefly vortex the tube and then do a quick spin (less than 5000xg) to concentrate the liquid at the bottom.
  5. Store the suspended plasmid at -20°C. The DNA is stable for at least one year from date of shipping when stored at -20°C.

Reference Data

• RefSeq: NM_001001798.1, NM_001001798.2, NP_001001798.1
• RefSeq Size: 5965 bp
• RefSeq ORF: 3351 bp
• Locus ID: 320940
• Cytogenetics: X A6
• MW: 128.3 kDa