Robo4 (NM_028783) Mouse Tagged ORF Clone

CAT#: MR227379

Robo4 (Myc-DDK-tagged) - Mouse roundabout homolog 4 (Drosophila) (Robo4)

Specifications

Product Data

• Type: Mouse Tagged ORF Clone
• Tag: Myc-DDK
• Symbol: Robo4
• Synonyms: 1200012D01Rik; AI593217
• Vector: pCMV6-Entry
• E. coli Selection: Kanamycin (25 ug/mL)
• Mammalian Cell Selection: Neomycin
• Sequence Data:
  ORF Nucleotide Sequence

  >MR227379 representing NM_028783
  Red=Cloning site Blue=ORF Green=Tags(s)
  
  TTTTGTAATACGACTCACTATAGGGCGGCCGGGAATTCGTCGACTGGATCCGGTACCGAGGAGATCTGCC
  GCCGCGATCGCC
  
  ATGGGACAAGGAGAGGAGCCGAGAGCAGCCATGGGCTCTGGAGGAACGGGCCTCCTGGGGACGGAGTGGC
  CTCTGCCTCTGCTGCTGCTTTTCATCATGGGAGGTGAGGCTCTGGATTCTCCACCCCAGATCCTAGTTCA
  CCCCCAGGACCAGCTACTTCAGGGCTCTGGCCCAGCCAAGATGAGGTGCAGATCATCCGGCCAACCACCT
  CCCACTATCCGCTGGCTGCTGAATGGGCAGCCCCTCAGCATGGCCACCCCAGACCTACATTACCTTTTGC
  CGGATGGGACCCTCCTGTTACATCGGCCCTCTGTCCAGGGACGGCCACAAGATGACCAGAACATCCTCTC
  AGCAATCCTGGGTGTCTACACATGTGAGGCCAGCAACCGGCTGGGCACAGCAGTGAGCCGGGGTGCTAGG
  CTGTCTGTGGCTGTCCTCCAGGAGGACTTCCAGATCCAACCTCGGGACACAGTGGCCGTGGTGGGAGAGA
  GCTTGGTTCTTGAGTGTGGTCCTCCCTGGGGCTACCCAAAACCCTCGGTCTCATGGTGGAAAGACGGGAA
  ACCCCTGGTCCTCCAGCCAGGGAGGCGCACAGTATCTGGGGATTCCCTGATGGTGTCAAGAGCAGAGAAG
  AATGACTCGGGGACCTATATGTGTATGGCCACCAACAATGCTGGGCAACGGGAGAGCCGAGCAGCCAGGG
  TGTCTATCCAGGAATCCCAGGACCACAAGGAACATCTAGAGCTTCTGGCTGTTCGCATTCAGCTGGAAAA
  TGTGACCCTGCTAAACCCCGAACCTGTAAAAGGTCCCAAGCCTGGGCCATCCGTGTGGCTCAGCTGGAAG
  GTGAGCGGCCCTGCTGCACCTGCTGAGTCATACACAGCTCTGTTCAGGACTCAGAGGTCCCCCAGGGACC
  AAGGATCTCCATGGACAGAGGTGCTGCTGCGTGGCTTGCAGAGTGCAAAGCTTGGGGGTCTCCACTGGGG
  CCAAGACTATGAATTCAAAGTGAGACCGTCCTCCGGCCGGGCTCGAGGCCCTGACAGCAATGTGTTGCTC
  CTGAGGCTGCCTGAACAGGTGCCCAGTGCCCCACCTCAAGGAGTGACCTTAAGATCTGGCAACGGTAGTG
  TCTTTGTGAGTTGGGCTCCACCACCTGCTGAAAGCCATAATGGTGTCATCCGTGGTTACCAGGTCTGGAG
  CCTGGGCAATGCCTCATTGCCTGCTGCCAACTGGACCGTAGTGGGTGAACAGACCCAGCTGGAGATCGCC
  ACACGACTGCCAGGCTCCTATTGTGTGCAAGTGGCTGCAGTCACTGGAGCTGGTGCTGGAGAACTCAGTA
  CCCCTGTCTGCCTCCTTTTAGAGCAGGCCATGGAGCAATCAGCACGAGACCCCAGGAAACATGTTCCCTG
  GACCCTGGAACAGCTGAGGGCCACCTTGAGACGACCAGAAGTCATTGCCAGTAGTGCTGTCCTACTCTGG
  TTGCTGCTACTAGGCATTACTGTGTGTATCTACAGACGACGCAAAGCTGGGGTGCACCTGGGCCCAGGTC
  TGTACAGATACACCAGCGAGGACGCCATTCTAAAACACAGGATGGACCACAGTGACTCCCCATGGCTGGC
  AGACACCTGGCGTTCCACCTCTGGCTCTCGAGACCTGAGCAGCAGCAGCAGCCTTAGTAGTCGGCTGGGA
  TTGGACCCTCGGGACCCACTAGAGGGCAGGCGCTCCTTGATCTCCTGGGACCCTCGGAGCCCCGGTGTAC
  CCCTGCTTCCAGACACGAGCACGTTTTACGGCTCCCTCATTGCAGAGCAGCCTTCCAGCCCTCCAGTCCG
  GCCAAGCCCCAAGACACCAGCTGCTAGGCGCTTTCCATCCAAGTTGGCTGGAACCTCCAGCCCCTGGGCT
  AGCTCAGATAGTCTCTGCAGCCGCAGGGGACTCTGTTCCCCACGCATGTCTCTGACCCCTACAGAGGCTT
  GGAAGGCCAAAAAGAAGCAGGAATTGCACCAAGCTAACAGCTCCCCACTGCTCCGGGGCAGCCACCCCAT
  GGAAATCTGGGCCTGGGAGTTGGGAAGCAGAGCCTCCAAGAACCTTTCTCAAAGCCCAGGAGAAGCGCCC
  CGAGCCGTGGTATCCTGGCGTGCTGTGGGACCACAACTTCACCGCAACTCCAGTGAGCTGGCATCTCGTC
  CACTCCCTCCAACACCCCTTTCTCTTCGTGGAGCTTCCAGTCATGACCCACAGAGCCAGTGTGTGGAGAA
  GCTCCAAGCTCCCTCCTCTGACCCACTGCCAGCAGCCCCTCTCTCCGTCCTCAACTCTTCCAGACCTTCC
  AGCCCCCAGGCCTCTTTCCTCTCCTGTCCTAGCCCATCCTCCAGCAACCTGTCCAGCTCCTCGCTGTCAT
  CCTTAGAGGAGGAGGAGGATCAGGACAGCGTGCTCACCCCCGAGGAGGTAGCCCTGTGTCTGGAGCTCAG
  TGATGGGGAGGAGACACCCACGAACAGTGTATCTCCTATGCCAAGAGCTCCTTCCCCGCCAACAACCTAT
  GGCTATATCAGCATACCAACCTGCTCAGGACTGGCAGACATGGGCAGAGCTGGCGGGGGCGTGGGGTCTG
  AGGTTGGGAACTTACTGTATCCACCTCGGCCCTGCCCCACCCCTACACCCAGCGAGGGCTCCCTGGCCAA
  TGGTTGGGGCTCAGCTTCTGAGGACAATGTCCCCAGCGCCAGGGCCAGCCTGGTTAGCTCTTCTGATGGC
  TCCTTCCTCGCTGATACTCACTTTGCTCGTGCCCTGGCAGTGGCTGTGGATAGCTTTGGCCTCAGTCTGG
  ATCCCAGGGAAGCTGACTGTGTCTTCACTGATGCCTCATCACCTCCCTCCCCTCGGGGTGATCTCTCCCT
  GACCCGAAGCTTCTCTCTGCCTTTGTGGGAGTGGAGGCCAGACTGGTTGGAAGATGCTGAGATCAGCCAC
  ACCCAGAGGCTGGGGAGGGGGCTGCCTCCCTGGCCTCCTGATTCTAGGGCCTCTTCCCAGCGAAGTTGGC
  TAACTGGTGCTGTGCCCAAGGCTGGTGATTCCTCC
  
  ACGCGTACGCGGCCGCTCGAGCAGAAACTCATCTCAGAAGAGGATCTGGCAGCAAATGATATCCTGGATT
  ACAAGGATGACGACGATAAGGTTTAA
  Protein Sequence

  >MR227379 representing NM_028783
  Red=Cloning site Green=Tags(s)
  
  MGQGEEPRAAMGSGGTGLLGTEWPLPLLLLFIMGGEALDSPPQILVHPQDQLLQGSGPAKMRCRSSGQPP
  PTIRWLLNGQPLSMATPDLHYLLPDGTLLLHRPSVQGRPQDDQNILSAILGVYTCEASNRLGTAVSRGAR
  LSVAVLQEDFQIQPRDTVAVVGESLVLECGPPWGYPKPSVSWWKDGKPLVLQPGRRTVSGDSLMVSRAEK
  NDSGTYMCMATNNAGQRESRAARVSIQESQDHKEHLELLAVRIQLENVTLLNPEPVKGPKPGPSVWLSWK
  VSGPAAPAESYTALFRTQRSPRDQGSPWTEVLLRGLQSAKLGGLHWGQDYEFKVRPSSGRARGPDSNVLL
  LRLPEQVPSAPPQGVTLRSGNGSVFVSWAPPPAESHNGVIRGYQVWSLGNASLPAANWTVVGEQTQLEIA
  TRLPGSYCVQVAAVTGAGAGELSTPVCLLLEQAMEQSARDPRKHVPWTLEQLRATLRRPEVIASSAVLLW
  LLLLGITVCIYRRRKAGVHLGPGLYRYTSEDAILKHRMDHSDSPWLADTWRSTSGSRDLSSSSSLSSRLG
  LDPRDPLEGRRSLISWDPRSPGVPLLPDTSTFYGSLIAEQPSSPPVRPSPKTPAARRFPSKLAGTSSPWA
  SSDSLCSRRGLCSPRMSLTPTEAWKAKKKQELHQANSSPLLRGSHPMEIWAWELGSRASKNLSQSPGEAP
  RAVVSWRAVGPQLHRNSSELASRPLPPTPLSLRGASSHDPQSQCVEKLQAPSSDPLPAAPLSVLNSSRPS
  SPQASFLSCPSPSSSNLSSSSLSSLEEEEDQDSVLTPEEVALCLELSDGEETPTNSVSPMPRAPSPPTTY
  GYISIPTCSGLADMGRAGGGVGSEVGNLLYPPRPCPTPTPSEGSLANGWGSASEDNVPSARASLVSSSDG
  SFLADTHFARALAVAVDSFGLSLDPREADCVFTDASSPPSPRGDLSLTRSFSLPLWEWRPDWLEDAEISH
  TQRLGRGLPPWPPDSRASSQRSWLTGAVPKAGDSS
  
  TRTRPLEQKLISEEDLAANDILDYKDDDDKV
• Restriction Sites: SgfI-MluI Cloning Scheme for this gene Plasmid Map
• ACCN: NM_028783
• ORF Size: 3045 bp
• OTI Disclaimer: The molecular sequence of this clone aligns with the gene accession number as a point of reference only. However, individual transcript sequences of the same gene can differ through naturally occurring variations (e.g. polymorphisms), each with its own valid existence. This clone is substantially in agreement with the reference, but a complete review of all prevailing variants is recommended prior to use. More info
• OTI Annotation: This clone was engineered to express the complete ORF with an expression tag. Expression varies depending on the nature of the gene.

Reference Data

• RefSeq: NM_028783.2, NM_028783.3, NP_083059.2
• RefSeq Size: 3689
• RefSeq ORF: 3018
• Locus ID: 74144
• MW: 109.4 kDa