Abcg8 (NM_026180) Mouse Tagged ORF Clone

CAT#: MR227387

Abcg8 (Myc-DDK-tagged) - Mouse ATP-binding cassette, sub-family G (WHITE), member 8 (Abcg8)

Specifications

Product Data

• Type: Mouse Tagged ORF Clone
• Tag: Myc-DDK
• Symbol: Abcg8
• Synonyms: 1300003C16Rik; AI114946; sterolin-2
• Vector: pCMV6-Entry
• E. coli Selection: Kanamycin (25 ug/mL)
• Mammalian Cell Selection: Neomycin
• Sequence Data:
  ORF Nucleotide Sequence

  >MR227387 representing NM_026180
  Red=Cloning site Blue=ORF Green=Tags(s)
  
  TTTTGTAATACGACTCACTATAGGGCGGCCGGGAATTCGTCGACTGGATCCGGTACCGAGGAGATCTGCC
  GCCGCGATCGCC
  
  ATGGCTGAGAAAACCAAAGAAGAGACCCAGCTGTGGAATGGGACTGTACTTCAGGATGCTTCGCAGGGCC
  TCCAGGACAGCTTGTTCTCCTCGGAAAGTGACAACAGTCTGTACTTCACCTACAGTGGTCAGTCCAACAC
  TCTGGAGGTCAGAGATCTCACCTACCAGGTGGACATCGCCTCTCAGGTGCCTTGGTTTGAGCAGCTGGCT
  CAGTTCAAGATACCCTGGAGGTCTCATAGCAGCCAAGACTCCTGTGAGCTGGGCATCCGAAATCTAAGCT
  TCAAAGTGAGGAGTGGACAGATGCTGGCCATCATAGGGAGCTCAGGCTGCGGGAGAGCCTCACTACTCGA
  CGTGATCACAGGCAGAGGCCACGGTGGCAAGATGAAATCAGGACAAATTTGGATAAATGGGCAACCCAGT
  ACGCCTCAGCTGGTGAGGAAGTGCGTTGCGCATGTGCGGCAGCATGACCAACTGCTGCCCAACCTGACCG
  TCAGAGAGACCCTGGCTTTCATTGCCCAGATGCGCCTGCCCAGGACCTTCTCCCAGGCCCAGCGTGACAA
  ACGGGTGGAAGACGTAATCGCCGAGCTGCGGCTGCGGCAGTGCGCCAACACCAGAGTGGGCAACACGTAT
  GTACGTGGGGTGTCCGGGGGTGAGCGCCGACGAGTGAGCATTGGGGTGCAGCTCCTGTGGAACCCAGGAA
  TCCTCATTCTGGATGAACCCACTTCTGGCCTCGACAGCTTCACAGCCCACAATCTGGTGACAACCTTGTC
  CCGCCTGGCCAAGGGCAACAGGCTGGTGCTCATCTCCCTCCACCAGCCTCGCTCTGACATCTTCAGGCTA
  TTTGACCTGGTCCTTCTGATGACATCTGGCACCCCTATCTACCTGGGGGCGGCGCAGCAAATGGTGCAGT
  ACTTCACATCCATTGGCCACCCTTGTCCTCGCTATAGCAACCCTGCGGACTTCTACGTGGACTTGACCAG
  CATCGACAGACGCAGCAAAGAACGGGAGGTGGCCACCGTGGAGAAGGCACAGTCTCTTGCAGCCCTGTTC
  CTAGAAAAAGTACAAGGCTTTGATGACTTTCTGTGGAAAGCTGAGGCAAAGGAACTCAACACAAGCACCC
  ACACAGTCAGCCTGACCCTCACACAGGACACTGACTGTGGGACTGCTGTTGAGCTGCCCGGGATGATAGA
  GCAGTTTTCCACCCTGATCCGTCGTCAGATTTCCAATGACTTCCGGGACCTGCCCACGCTGCTCATTCAT
  GGGTCGGAAGCCTGCCTGATGTCCCTCATCATTGGCTTCCTTTACTACGGCCATGGGGCCAAGCAGCTCT
  CCTTCATGGACACAGCAGCCCTCCTCTTCATGATAGGGGCGCTCATTCCTTTCAATGTCATCCTGGATGT
  CGTCTCCAAATGTCACTCGGAGAGGTCAATGCTGTACTATGAGCTGGAAGACGGGCTGTACACTGCTGGT
  CCTTATTTCTTTGCCAAGATCCTAGGAGAATTGCCGGAGCACTGTGCCTACGTCATCATCTACGCGATGC
  CCATCTACTGGCTGACAAACCTGCGGCCCGTGCCTGAGCTCTTCCTTCTACACTTCCTGCTCGTGTGGTT
  GGTGGTCTTCTGCTGCAGGACCATGGCCCTGGCTGCCTCTGCCATGCTGCCCACCTTCCACATGTCCTCC
  TTCTTCTGCAATGCCCTCTACAACTCCTTCTACCTTACTGCCGGCTTCATGATAAACTTGGACAACCTGT
  GGATAGTGCCTGCATGGATCTCCAAGCTGTCGTTCCTCCGGTGGTGCTTCTCGGGGCTGATGCAGATTCA
  ATTTAATGGACACCTTTACACCACACAAATCGGCAACTTCACCTTCTCCATCCTCGGAGACACGATGATC
  AGTGCCATGGACCTGAACTCGCATCCACTCTATGCGATCTACCTCATTGTCATCGGCATCAGCTACGGCT
  TCCTGTTCCTGTACTATCTATCCTTGAAGCTCATCAAACAGAAGTCAATTCAAGACTGG
  
  ACGCGTACGCGGCCGCTCGAGCAGAAACTCATCTCAGAAGAGGATCTGGCAGCAAATGATATCCTGGATT
  ACAAGGATGACGACGATAAGGTTTAA
  Protein Sequence

  >MR227387 representing NM_026180
  Red=Cloning site Green=Tags(s)
  
  MAEKTKEETQLWNGTVLQDASQGLQDSLFSSESDNSLYFTYSGQSNTLEVRDLTYQVDIASQVPWFEQLA
  QFKIPWRSHSSQDSCELGIRNLSFKVRSGQMLAIIGSSGCGRASLLDVITGRGHGGKMKSGQIWINGQPS
  TPQLVRKCVAHVRQHDQLLPNLTVRETLAFIAQMRLPRTFSQAQRDKRVEDVIAELRLRQCANTRVGNTY
  VRGVSGGERRRVSIGVQLLWNPGILILDEPTSGLDSFTAHNLVTTLSRLAKGNRLVLISLHQPRSDIFRL
  FDLVLLMTSGTPIYLGAAQQMVQYFTSIGHPCPRYSNPADFYVDLTSIDRRSKEREVATVEKAQSLAALF
  LEKVQGFDDFLWKAEAKELNTSTHTVSLTLTQDTDCGTAVELPGMIEQFSTLIRRQISNDFRDLPTLLIH
  GSEACLMSLIIGFLYYGHGAKQLSFMDTAALLFMIGALIPFNVILDVVSKCHSERSMLYYELEDGLYTAG
  PYFFAKILGELPEHCAYVIIYAMPIYWLTNLRPVPELFLLHFLLVWLVVFCCRTMALAASAMLPTFHMSS
  FFCNALYNSFYLTAGFMINLDNLWIVPAWISKLSFLRWCFSGLMQIQFNGHLYTTQIGNFTFSILGDTMI
  SAMDLNSHPLYAIYLIVIGISYGFLFLYYLSLKLIKQKSIQDW
  
  TRTRPLEQKLISEEDLAANDILDYKDDDDKV
• Restriction Sites: SgfI-MluI Cloning Scheme for this gene Plasmid Map
• ACCN: NM_026180
• ORF Size: 2019 bp
• OTI Disclaimer: Due to the inherent nature of this plasmid, standard methods to replicate additional amounts of DNA in E. coli are highly likely to result in mutations and/or rearrangements. Therefore, OriGene does not guarantee the capability to replicate this plasmid DNA. Additional amounts of DNA can be purchased from OriGene with batch-specific, full-sequence verification at a reduced cost. Please contact our customer care team at custsupport@origene.com or by calling 301.340.3188 option 3 for pricing and delivery.
  
  The molecular sequence of this clone aligns with the gene accession number as a point of reference only. However, individual transcript sequences of the same gene can differ through naturally occurring variations (e.g. polymorphisms), each with its own valid existence. This clone is substantially in agreement with the reference, but a complete review of all prevailing variants is recommended prior to use. More info
• OTI Annotation: This clone was engineered to express the complete ORF with an expression tag. Expression varies depending on the nature of the gene.

Reference Data

• RefSeq: NM_026180.1, NM_026180.2, NM_026180.3, NP_080456.1
• RefSeq Size: 3674
• RefSeq ORF: 2022
• Locus ID: 67470
• MW: 76.4 kDa
• Gene Summary: Transporter that appears to play an indispensable role in the selective transport of the dietary cholesterol in and out of the enterocytes and in the selective sterol excretion by the liver into bile. [UniProtKB/Swiss-Prot Function]