Nos1 (NM_008712) Mouse Tagged ORF Clone

CAT#: MR227419

Nos1 (Myc-DDK-tagged) - Mouse nitric oxide synthase 1, neuronal (Nos1)

Specifications

Product Data

• Type: Mouse Tagged ORF Clone
• Tag: Myc-DDK
• Symbol: Nos1
• Synonyms: 2310005C01Rik; bNOS; N-NOS; NC-NOS; nNOS; NO; NOS; Nos-1; NOS-I
• Vector: pCMV6-Entry
• E. coli Selection: Kanamycin (25 ug/mL)
• Mammalian Cell Selection: Neomycin
• Sequence Data:
  ORF Nucleotide Sequence

  >MR227419 representing NM_008712
  Red=Cloning site Blue=ORF Green=Tags(s)
  
  TTTTGTAATACGACTCACTATAGGGCGGCCGGGAATTCGTCGACTGGATCCGGTACCGAGGAGATCTGCC
  GCCGCGATCGCC
  
  ATGGAAGAGCACACGTTTGGGGTCCAGCAGATCCAACCCAACGTCATTTCTGTCCGTCTCTTCAAACGCA
  AAGTGGGAGGTCTGGGCTTCCTGGTGAAGGAACGGGTCAGCAAGCCCCCGGTGATCATCTCAGATCTGAT
  CCGAGGAGGTGCCGCGGAGCAGAGCGGCCTTATCCAAGCCGGCGACATCATTCTCGCCGTCAATGATCGG
  CCCCTGGTAGACCTCAGCTATGACAGCGCTCTGGAAGTTCTCAGGGGCATTGCCTCTGAGACCCATGTGG
  TCCTCATTCTGAGAGGCCCTGAGGGCTTTACTACACACCTGGAGACCACCTTCACAGGGGATGGAACCCC
  CAAGACCATCCGTGTGACCCAGCCCCTGGGGACACCCACCAAAGCTGTCGATCTGTCTCGCCAGCCATCA
  GCCAGCAAAGACCAGCCATTAGCAGTAGACAGGGTCCCAGGTCCCAGTAACGGACCTCAGCATGCCCAAG
  GCCGTGGACAGGGAGCCGGCTCAGTCTCCCAGGCTAATGGTGTGGCCATTGACCCCACAATGAAAAATAC
  CAAGGCCAACCTCCAGGACAGCGGAGAACAGGATGAACTGCTCAAAGAGATAGAACCTGTGTTGAGCATC
  CTCACCGGTGGGGGAAAAGCAGTCAACAGAGGGGGACCAGCCAAAGCAGAGATGAAAGACACAGGAATCC
  AGGTGGACAGAGACCTCGACGGCAAACTGCACAAAGCTCCGCCCCTGGGCGGGGAGAATGATCGAGTCTT
  CAATGATCTGTGGGGGAAGGGCAACGTCCCTGTGGTCCTCAACAACCCGTATTCAGAGAACGAACAGTCT
  CCCGCCTCGGGCAAACAGTCTCCTACCAAGAATGGCAGTCCTTCCAGGTGCCCTCGCTTCCTTAAGGTCA
  AGAACTGGGAGACAGATGTGGTCCTCACTGACACCCTGCACCTGAAGAGCACGCTGGAAACGGGGTGCAC
  AGAGCAGATCTGCATGGGCTCCATCATGCTGCCATCCCATCACATACGGAAGTCAGAAGATGTCCGCACC
  AAGGACCAGCTCTTCCCTCTAGCCAAAGAATTTCTCGACCAATACTACTCCTCCATTAAGAGGTTTGGCT
  CCAAGGCCCACATGGACAGGCTGGAGGAGGTGAACAAGGAGATCGAGAGCACCAGCACCTACCAGCTCAA
  GGACACGGAGCTCATCTATGGCGCCAAGCATGCCTGGCGGAACGCCTCTCGCTGTGTGGGCAGGATCCAG
  TGGTCCAAGCTGCAGGTGTTCGATGCCCGAGACTGCACCACAGCCCATGGGATGTTCAACTACATCTGTA
  ACCACGTCAAGTACGCCACCAACAAAGGGAACCTCAGGTCGGCCATCACTATATTCCCTCAAAGGACTGA
  TGGCAAGCATGACTTCCGAGTGTGGAACTCGCAGCTCATCCGCTATGCCGGCTACAAGCAGCCAGATGGC
  TCTACCTTGGGCGATCCAGCTAATGTGGAGTTCACAGAGATCTGTATACAGCAGGGCTGGAAACCCCCAA
  GAGGCCGGTTCGATGTGCTGCCTCTCCTGCTCCAGGCCAATGGCAATGACCCTGAACTCTTTCAGATCCC
  CCCAGAGCTGGTGCTGGAAGTACCCATCAGGCACCCCAAGTTCGACTGGTTTAAGGACCTGGGGCTCAAA
  TGGTATGGCCTCCCCGCTGTGTCCAACATGCTGCTGGAGATTGGGGGCCTGGAGTTCAGCGCCTGTCCCT
  TTAGTGGCTGGTACATGGGCACGGAGATTGGCGTTCGTGATTACTGTGACAACTCTCGATACAACATCCT
  GGAGGAAGTAGCCAAGAAAATGGATTTGGACATGAGGAAGACATCCTCCCTCTGGAAGGACCAAGCCCTG
  GTGGAGATTAACATTGCTGTCCTATACAGCTTCCAGAGTGACAAGGTGACCATTGTGGACCACCACTCTG
  CCACCGAGTCCTTCATCAAACACATGGAGAATGAGTACCGCTGCAGAGGGGGCTGTCCCGCCGACTGGGT
  GTGGATTGTGCCCCCCATGTCGGGCAGCATCACCCCTGTCTTCCACCAGGAGATGCTCAACTACCGGCTC
  ACCCCGTCCTTTGAGTACCAGCCTGATCCATGGAACACCCACGTGTGGAAGGGCACCAATGGGACCCCCA
  CGAAGCGGCGAGCCATTGGCTTCAAGAAATTGGCAGAGGCCGTCAAGTTCTCAGCCAAGCTGATGGGACA
  GGCCATGGCCAAGAGAGTCAAAGCGACCATTCTCTACGCCACCGAGACAGGCAAATCCCAAGCCTATGCC
  AAGACCCTGTGCGAGATCTTCAAGCATGCCTTCGATGCCAAGGCTATGTCCATGGAGGAATATGACATCG
  TGCACCTGGAGCACGAAGCCCTGGTCTTGGTGGTCACCAGCACCTTTGGCAATGGAGACCCCCCAGAGAA
  TGGGGAGAAATTCGGCTGTGCTTTGATGGAGATGAGGCACCCCAACTCTGTGCAAGAGGAGAGGAAGAGC
  TACAAGGTCCGATTCAACAGCGTCTCCTCCTATTCTGACTCCCGCAAGTCATCAGGCGATGGACCAGACC
  TCAGAGACAACTTTGAAAGTACTGGACCGCTGGCCAATGTGAGGTTCTCAGTCTTCGGCCTGGGCTCTCG
  GGCATACCCTCACTTCTGTGCCTTTGGGCACGCAGTGGACACTCTCCTGGAGGAGCTGGGCGGGGAGAGG
  ATTCTGAAGATGAGGGAGGGAGATGAGCTCTGCGGACAGGAAGAGGCTTTCAGGACCTGGGCTAAGAAAG
  TCTTCAAGGCAGCCTGTGATGTGTTCTGTGTGGGGGATGACGTCAACATTGAGAAGGCGAACAATTCCCT
  CATTAGCAATGACCGCAGCTGGAAGAGGAATAAATTCCGCCTCACTTATGTGGCAGAAGCTCCAGAGCTG
  ACCCAAGGTCTTTCCAATGTTCACAAAAAGCGAGTCTCAGCCGCCCGACTCCTCAGCCGCCAAAACCTGC
  AAAGTCCTAAATCCAGCCGATCGACCATCTTCGTGCGTCTCCACACCAACGGGAATCAGGAGTTGCAGTA
  CCAGCCAGGGGACCACCTGGGTGTCTTCCCTGGCAACCATGAGGACCTCGTGAATGCACTCATTGAACGG
  CTGGAGGACGCGCCGCCTGCCAACCACGTGGTGAAGGTGGAAATGCTGGAGGAAAGGAACACGGCTCTGG
  GTGTCATCAGTAATTGGAAGGATGAATCTCGCCTCCCACCCTGCACCATCTTCCAGGCCTTCAAGTACTA
  CCTGGACATCACCACGCCGCCCACGCCCCTGCAGCTGCAGCAGTTCGCCTCCCTGGCCACCAATGAGAAA
  GAGAAGCAGCGGCTGCTGGTCCTCAGCAAGGGGCTTCAGGAATATGAGGAATGGAAATGGGGCAAGAACC
  CCACAATGGTGGAGGTGCTGGAGGAGTTCCCGTCCATCCAGATGCCGGCCACACTTCTCCTCACACAGCT
  GTCGCTGCTACAACCTCGCTACTATTCCATCAGCTCCTCTCCAGACATGTACCCCGACGAGGTGCACCTC
  ACCGTGGCCATCGTGTCCTACCATACCCGAGATGGAGAAGGACCGGTTCACCACGGGGTGTGCTCCTCCT
  GGCTCAACCGAATACAGGCTGACGATGTCGTCCCCTGCTTCGTGAGAGGTGCCCCTAGCTTCCACCTGCC
  TCGAAACCCCCAGGTGCCTTGCATCCTGGTTGGCCCAGGCACCGGCATCGCTCCCTTCCGAAGTTTTTGG
  CAACAGCGACAATTTGACATCCAACACAAAGGAATGAATCCGTGCCCCATGGTTCTGGTCTTCGGGTGTC
  GACAATCCAAGATAGATCATATCTACAGAGAGGAGACCCTGCAGGCCAAGAACAAGGGCGTCTTCAGAGA
  GCTGTACACTGCCTACTCCCGGGAACCCGACAGGCCAAAGAAATATGTACAGGACGTGCTGCAGGAACAG
  CTGGCCGAGTCTGTGTACCGCGCCCTGAAGGAGCAAGGAGGCCATATTTATGTCTGCGGGGACGTTACCA
  TGGCCGCTGATGTCCTCAAAGCCATCCAGCGCATAATGACCCAGCAGGGGAAACTCTCGGAGGAGGATGC
  TGGTGTGTTCATCAGCAGGCTGAGGGATGACAACCGGTACCACGAGGACATCTTTGGGGTCACCCTCAGA
  ACATATGAAGTGACCAACCGCCTTAGATCTGAGTCCATCGCCTTCATTGAAGAGAGCAAAAAAGACACAG
  ATGAGGTTTTCAGCTCC
  
  ACGCGTACGCGGCCGCTCGAGCAGAAACTCATCTCAGAAGAGGATCTGGCAGCAAATGATATCCTGGATT
  ACAAGGATGACGACGATAAGGTTTAA
  Protein Sequence

  >MR227419 representing NM_008712
  Red=Cloning site Green=Tags(s)
  
  MEEHTFGVQQIQPNVISVRLFKRKVGGLGFLVKERVSKPPVIISDLIRGGAAEQSGLIQAGDIILAVNDR
  PLVDLSYDSALEVLRGIASETHVVLILRGPEGFTTHLETTFTGDGTPKTIRVTQPLGTPTKAVDLSRQPS
  ASKDQPLAVDRVPGPSNGPQHAQGRGQGAGSVSQANGVAIDPTMKNTKANLQDSGEQDELLKEIEPVLSI
  LTGGGKAVNRGGPAKAEMKDTGIQVDRDLDGKLHKAPPLGGENDRVFNDLWGKGNVPVVLNNPYSENEQS
  PASGKQSPTKNGSPSRCPRFLKVKNWETDVVLTDTLHLKSTLETGCTEQICMGSIMLPSHHIRKSEDVRT
  KDQLFPLAKEFLDQYYSSIKRFGSKAHMDRLEEVNKEIESTSTYQLKDTELIYGAKHAWRNASRCVGRIQ
  WSKLQVFDARDCTTAHGMFNYICNHVKYATNKGNLRSAITIFPQRTDGKHDFRVWNSQLIRYAGYKQPDG
  STLGDPANVEFTEICIQQGWKPPRGRFDVLPLLLQANGNDPELFQIPPELVLEVPIRHPKFDWFKDLGLK
  WYGLPAVSNMLLEIGGLEFSACPFSGWYMGTEIGVRDYCDNSRYNILEEVAKKMDLDMRKTSSLWKDQAL
  VEINIAVLYSFQSDKVTIVDHHSATESFIKHMENEYRCRGGCPADWVWIVPPMSGSITPVFHQEMLNYRL
  TPSFEYQPDPWNTHVWKGTNGTPTKRRAIGFKKLAEAVKFSAKLMGQAMAKRVKATILYATETGKSQAYA
  KTLCEIFKHAFDAKAMSMEEYDIVHLEHEALVLVVTSTFGNGDPPENGEKFGCALMEMRHPNSVQEERKS
  YKVRFNSVSSYSDSRKSSGDGPDLRDNFESTGPLANVRFSVFGLGSRAYPHFCAFGHAVDTLLEELGGER
  ILKMREGDELCGQEEAFRTWAKKVFKAACDVFCVGDDVNIEKANNSLISNDRSWKRNKFRLTYVAEAPEL
  TQGLSNVHKKRVSAARLLSRQNLQSPKSSRSTIFVRLHTNGNQELQYQPGDHLGVFPGNHEDLVNALIER
  LEDAPPANHVVKVEMLEERNTALGVISNWKDESRLPPCTIFQAFKYYLDITTPPTPLQLQQFASLATNEK
  EKQRLLVLSKGLQEYEEWKWGKNPTMVEVLEEFPSIQMPATLLLTQLSLLQPRYYSISSSPDMYPDEVHL
  TVAIVSYHTRDGEGPVHHGVCSSWLNRIQADDVVPCFVRGAPSFHLPRNPQVPCILVGPGTGIAPFRSFW
  QQRQFDIQHKGMNPCPMVLVFGCRQSKIDHIYREETLQAKNKGVFRELYTAYSREPDRPKKYVQDVLQEQ
  LAESVYRALKEQGGHIYVCGDVTMAADVLKAIQRIMTQQGKLSEEDAGVFISRLRDDNRYHEDIFGVTLR
  TYEVTNRLRSESIAFIEESKKDTDEVFSS
  
  TRTRPLEQKLISEEDLAANDILDYKDDDDKV
• Restriction Sites: SgfI-MluI Cloning Scheme for this gene Plasmid Map
• ACCN: NM_008712
• ORF Size: 4287 bp
• OTI Disclaimer: The molecular sequence of this clone aligns with the gene accession number as a point of reference only. However, individual transcript sequences of the same gene can differ through naturally occurring variations (e.g. polymorphisms), each with its own valid existence. This clone is substantially in agreement with the reference, but a complete review of all prevailing variants is recommended prior to use. More info
• OTI Annotation: This clone was engineered to express the complete ORF with an expression tag. Expression varies depending on the nature of the gene.

Reference Data

• RefSeq: NM_008712.1, NM_008712.2, NM_008712.3, NP_032738.1
• RefSeq Size: 4388
• RefSeq ORF: 4290
• Locus ID: 18125
• MW: 160.9 kDa