Sema4d (NM_013660) Mouse Tagged ORF Clone

CAT#: MR227571

Sema4d (Myc-DDK-tagged) - Mouse sema domain, immunoglobulin domain (Ig), transmembrane domain (TM) and short cytoplasmic domain, (semaphorin) 4D (Sema4d)

Specifications

Product Data

• Type: Mouse Tagged ORF Clone
• Tag: Myc-DDK
• Symbol: Sema4d
• Synonyms: CD100; coll-4; Semacl2; Semaj; Semcl2
• Vector: pCMV6-Entry
• E. coli Selection: Kanamycin (25 ug/mL)
• Mammalian Cell Selection: Neomycin
• Sequence Data:
  ORF Nucleotide Sequence

  >MR227571 ORF sequence
  Red=Cloning site Blue=ORF Green=Tags(s)
  
  TTTTGTAATACGACTCACTATAGGGCGGCCGGGAATTCGTCGACTGGATCCGGTACCGAGGAGATCTGCC
  GCCGCGATCGCC
  
  ATGAGGATGTGTGCCCCCGTTAGGGGGCTGTTCTTGGCCCTGGTGGTAGTGTTGAGAACCGCGGTGGCAT
  TTGCACCTGTGCCTCGGCTCACCTGGGAACATGGAGAGGTAGGTCTGGTGCAGTTTCACAAGCCAGGCAT
  CTTTAACTACTCGGCCTTGCTGATGAGTGAGGACAAAGACACTCTGTATGTAGGCGCCCGGGAAGCAGTC
  TTTGCAGTGAATGCGCTGAACATCTCTGAGAAGCAACATGAGGTATATTGGAAGGTCTCTGAAGACAAAA
  AATCCAAGTGTGCAGAGAAGGGGAAATCAAAGCAGACGGAATGCCTAAACTACATTCGAGTACTACAGCC
  ACTAAGCAGCACTTCCCTCTATGTGTGTGGGACCAATGCGTTCCAGCCCACCTGTGACCACCTGAACTTG
  ACATCCTTCAAGTTTCTGGGGAAAAGTGAAGATGGCAAAGGAAGATGCCCCTTCGACCCCGCCCACAGCT
  ACACATCAGTCATGGTTGGGGGCGAGCTCTACTCTGGGACGTCCTATAATTTCTTGGGCAGTGAACCCAT
  CATCTCTCGAAACTCTTCCCACAGTCCCTTGAGGACGGAGTATGCCATCCCGTGGCTGAACGAGCCTAGC
  TTCGTCTTTGCTGACGTGATCCAGAAAAGCCCAGATGGTCCGGAGGGTGAAGATGACAAGGTCTACTTCT
  TTTTTACGGAGGTATCCGTGGAGTACGAATTCGTCTTCAAGTTGATGATCCCGCGAGTTGCCAGGGTGTG
  CAAGGGCGACCAGGGCGGCCTGCGGACTTTGCAAAAAAAGTGGACCTCCTTCCTAAAGGCCAGGCTGATC
  TGCTCCAAGCCAGACAGTGGCCTGGTCTTCAACATACTTCAGGATGTGTTTGTGCTGAGGGCCCCGGGCC
  TCAAGGAGCCTGTGTTCTATGCGGTCTTCACCCCACAGCTGAACAATGTGGGTCTGTCAGCGGTGTGCGC
  CTACACACTGGCCACGGTGGAGGCAGTCTTCTCCCGTGGAAAGTACATGCAGAGTGCCACAGTGGAGCAG
  TCTCACACCAAGTGGGTGCGCTACAATGGCCCAGTGCCCACTCCCCGACCTGGAGCGTGTATCGACAGTG
  AGGCCCGGGCAGCCAACTACACCAGCTCCTTGAATCTCCCAGACAAAACACTGCAGTTTGTAAAAGACCA
  CCCTTTGATGGATGACTCAGTGACCCCGATAGACAACAGACCCAAGCTGATCAAAAAAGATGTAAACTAC
  ACCCAGATAGTGGTAGACAGGACCCAGGCCCTGGATGGGACTTTCTACGACGTCATGTTCATCAGCACAG
  ACCGGGGAGCTCTGCATAAAGCAGTCATCCTCACAAAAGAGGTGCATGTCATCGAGGAGACCCAACTCTT
  CCGGGACTCTGAACCGGTCCTAACTCTGCTGCTATCGTCAAAGAAGGGGAGGAAGTTTGTCTATGCAGGC
  TCCAACTCTGGAGTGGTCCAAGCGCCCCTGGCATTCTGCGAAAAGCACGGTAGCTGTGAAGACTGTGTGT
  TAGCACGGGACCCCTACTGTGCCTGGAGCCCAGCCATCAAGGCCTGTGTTACCCTGCACCAGGAAGAGGC
  CTCCAGCAGGGGCTGGATTCAGGACATGAGCGGTGACACATCCTCATGCCTGGATAAGAGTAAAGAAAGT
  TTCAACCAGCATTTTTTCAAGCACGGCGGCACAGCGGAACTCAAATGTTTCCAAAAGTCCAACCTAGCCC
  GGGTGGTATGGAAGTTCCAGAATGGCGAGTTGAAGGCCGCAAGTCCCAAGTACGGCTTTGTGGGCAGGAA
  GCACCTGCTCATCTTCAACCTGTCGGACGGAGACAGCGGCGTGTACCAGTGCCTGTCAGAGGAAAGGGTG
  AGGAATAAAACGGTCTCCCAGCTGCTGGCCAAGCACGTTCTGGAAGTGAAGATGGTACCTCGGACCCCCC
  CCTCACCTACCTCAGAGGATGCTCAGACAGAAGGTAGTAAGATCACATCCAAAATGCCGGTTGCATCTAC
  CCAGGGGTCCTCTCCCCCTACCCCGGCTCTGTGGGCAACCTCCCCCAGAGCCGCCACCCTACCTCCCAAG
  TCCTCCTCCGGCACATCCTGTGAACCAAAGATGGTCATCAACACGGTCCCCCAGCTCCACTCAGAGAAGA
  CGGTGTATCTCAAGTCCAGTGACAACCGCCTGCTCATGTCTCTCCTCCTCTTCATCTTTGTCCTCTTCCT
  CTGCCTCTTTTCCTACAACTGCTACAAGGGCTACCTGCCCGGACAGTGCTTAAAATTCCGCTCAGCCCTG
  CTGCTTGGAAAGAAAACACCCAAGTCAGACTTCTCTGACCTGGAGCAGAGTGTGAAGGAGACACTGGTCG
  AGCCTGGGAGCTTCTCCCAGCAGAACGGCGACCACCCCAAGCCAGCCCTGGATACGGGCTATGAAACGGA
  GCAGGACACCATCACCAGCAAAGTCCCCACGGATCGTGAGGACTCGCAACGGATCGATGAACTCTCTGCC
  CGGGACAAACCGTTTGATGTCAAGTGTGAACTGAAGTTTGCAGATTCGGATGCTGACGGGGAC
  
  ACGCGTACGCGGCCGCTCGAGCAGAAACTCATCTCAGAAGAGGATCTGGCAGCAAATGATATCCTGGATT
  ACAAGGATGACGACGATAAGGTTTAA
  Protein Sequence

  >MR227571 protein sequence
  Red=Cloning site Green=Tags(s)
  
  MRMCAPVRGLFLALVVVLRTAVAFAPVPRLTWEHGEVGLVQFHKPGIFNYSALLMSEDKDTLYVGAREAV
  FAVNALNISEKQHEVYWKVSEDKKSKCAEKGKSKQTECLNYIRVLQPLSSTSLYVCGTNAFQPTCDHLNL
  TSFKFLGKSEDGKGRCPFDPAHSYTSVMVGGELYSGTSYNFLGSEPIISRNSSHSPLRTEYAIPWLNEPS
  FVFADVIQKSPDGPEGEDDKVYFFFTEVSVEYEFVFKLMIPRVARVCKGDQGGLRTLQKKWTSFLKARLI
  CSKPDSGLVFNILQDVFVLRAPGLKEPVFYAVFTPQLNNVGLSAVCAYTLATVEAVFSRGKYMQSATVEQ
  SHTKWVRYNGPVPTPRPGACIDSEARAANYTSSLNLPDKTLQFVKDHPLMDDSVTPIDNRPKLIKKDVNY
  TQIVVDRTQALDGTFYDVMFISTDRGALHKAVILTKEVHVIEETQLFRDSEPVLTLLLSSKKGRKFVYAG
  SNSGVVQAPLAFCEKHGSCEDCVLARDPYCAWSPAIKACVTLHQEEASSRGWIQDMSGDTSSCLDKSKES
  FNQHFFKHGGTAELKCFQKSNLARVVWKFQNGELKAASPKYGFVGRKHLLIFNLSDGDSGVYQCLSEERV
  RNKTVSQLLAKHVLEVKMVPRTPPSPTSEDAQTEGSKITSKMPVASTQGSSPPTPALWATSPRAATLPPK
  SSSGTSCEPKMVINTVPQLHSEKTVYLKSSDNRLLMSLLLFIFVLFLCLFSYNCYKGYLPGQCLKFRSAL
  LLGKKTPKSDFSDLEQSVKETLVEPGSFSQQNGDHPKPALDTGYETEQDTITSKVPTDREDSQRIDELSA
  RDKPFDVKCELKFADSDADGD
  
  TRTRPLEQKLISEEDLAANDILDYKDDDDKV
• Restriction Sites: SgfI-MluI Cloning Scheme for this gene Plasmid Map
• ACCN: NM_013660
• ORF Size: 2586 bp
• OTI Disclaimer: Due to the inherent nature of this plasmid, standard methods to replicate additional amounts of DNA in E. coli are highly likely to result in mutations and/or rearrangements. Therefore, OriGene does not guarantee the capability to replicate this plasmid DNA. Additional amounts of DNA can be purchased from OriGene with batch-specific, full-sequence verification at a reduced cost. Please contact our customer care team at custsupport@origene.com or by calling 301.340.3188 option 3 for pricing and delivery.
  
  The molecular sequence of this clone aligns with the gene accession number as a point of reference only. However, individual transcript sequences of the same gene can differ through naturally occurring variations (e.g. polymorphisms), each with its own valid existence. This clone is substantially in agreement with the reference, but a complete review of all prevailing variants is recommended prior to use. More info
• OTI Annotation: This clone was engineered to express the complete ORF with an expression tag. Expression varies depending on the nature of the gene.

Reference Data

• RefSeq: NM_013660.1, NM_013660.2, NM_013660.3, NM_013660.4, NP_038688.2
• RefSeq Size: 4491
• RefSeq ORF: 2586
• Locus ID: 20354
• MW: 95.6 kDa