Gpbar1 (NM_174985) Mouse Tagged ORF Clone

CAT#: MR227683

Gpbar1 (Myc-DDK-tagged) - Mouse G protein-coupled bile acid receptor 1 (Gpbar1)

Specifications

Product Data

• Type: Mouse Tagged ORF Clone
• Tag: Myc-DDK
• Symbol: Gpbar1
• Synonyms: BG37; GPCR; GPR131; M-BAR; TGR5
• Vector: pCMV6-Entry
• E. coli Selection: Kanamycin (25 ug/mL)
• Mammalian Cell Selection: Neomycin
• Sequence Data:
  ORF Nucleotide Sequence

  >MR227683 representing NM_174985
  Red=Cloning site Blue=ORF Green=Tags(s)
  
  TTTTGTAATACGACTCACTATAGGGCGGCCGGGAATTCGTCGACTGGATCCGGTACCGAGGAGATCTGCC
  GCCGCGATCGCC
  
  ATGATGACACCCAACAGCACTGAGCTGTCGGCCATTCCCATGGGGGTTCTGGGGCTTTCCTTGGCCCTGG
  CAAGCCTCATCGTCATCGCCAACCTGCTCCTGGCCCTAGGCATCGCCCTGGACCGCCACTTGCGCAGCCC
  ACCTGCTGGCTGCTTCTTCCTAAGCCTACTACTAGCCGGGCTGCTCACAGGGCTGGCACTGCCCATGCTG
  CCTGGGCTATGGAGCCGGAACCATCAGGGCTACTGGTCCTGCCTCCTTCTCCACTTGACCCCCAACTTTT
  GTTTCCTTTCCCTGCTTGCCAATCTGCTGCTGGTGCATGGGGAACGCTACATGGCAGTGTTGCAGCCACT
  CCGGCCCCATGGAAGTGTGCGGCTAGCCCTGTTCCTCACCTGGGTCAGCTCCCTGTTCTTTGCCAGCCTG
  CCTGCTCTGGGCTGGAACCATTGGAGCCCTGATGCCAACTGCAGCTCCCAAGCTGTCTTCCCAGCCCCCT
  ACCTCTACCTGGAAGTTTATGGCCTCCTGTTGCCTGCCGTGGGGGCCACTGCCCTTCTCTCTGTCCGCGT
  GTTGGCCACTGCCCACCGCCAGCTGTGTGAGATCCGCCGACTGGAGCGGGCAGTGTGCCGCGATGTACCC
  TCAACCCTGGCTAGGGCTCTCACCTGGAGGCAGGCTAGGGCACAGGCAGGAGCCACACTGCTCTTCTTGC
  TGTGTTGGGGGCCCTATGTGGCCACATTGCTCCTGTCAGTCTTGGCCTATGAGCGTCGCCCACCACTAGG
  GCCTGGAACTCTGTTATCGCTCATCTCATTGGGCAGCACCAGTGCTGCCGCTGTGCCTGTGGCCATGGGG
  CTGGGTGATCAGCGCTACACAGCCCCCTGGAGGACAGCTGCCCAAAGGTGTCTACGAGTGCTTCGAGGAA
  GAGCCAAGAGGGACAATCCAGGCCCCAGCACTGCCTACCACACCAGTAGCCAATGCAGCATTGACCTGGA
  CTTGAAT
  
  ACGCGTACGCGGCCGCTCGAGCAGAAACTCATCTCAGAAGAGGATCTGGCAGCAAATGATATCCTGGATT
  ACAAGGATGACGACGATAAGGTTTAA
  Protein Sequence

  >MR227683 representing NM_174985
  Red=Cloning site Green=Tags(s)
  
  MMTPNSTELSAIPMGVLGLSLALASLIVIANLLLALGIALDRHLRSPPAGCFFLSLLLAGLLTGLALPML
  PGLWSRNHQGYWSCLLLHLTPNFCFLSLLANLLLVHGERYMAVLQPLRPHGSVRLALFLTWVSSLFFASL
  PALGWNHWSPDANCSSQAVFPAPYLYLEVYGLLLPAVGATALLSVRVLATAHRQLCEIRRLERAVCRDVP
  STLARALTWRQARAQAGATLLFLLCWGPYVATLLLSVLAYERRPPLGPGTLLSLISLGSTSAAAVPVAMG
  LGDQRYTAPWRTAAQRCLRVLRGRAKRDNPGPSTAYHTSSQCSIDLDLN
  
  TRTRPLEQKLISEEDLAANDILDYKDDDDKV
• Restriction Sites: SgfI-MluI Cloning Scheme for this gene Plasmid Map
• ACCN: NM_174985
• ORF Size: 987 bp
• OTI Disclaimer: The molecular sequence of this clone aligns with the gene accession number as a point of reference only. However, individual transcript sequences of the same gene can differ through naturally occurring variations (e.g. polymorphisms), each with its own valid existence. This clone is substantially in agreement with the reference, but a complete review of all prevailing variants is recommended prior to use. More info
• OTI Annotation: This clone was engineered to express the complete ORF with an expression tag. Expression varies depending on the nature of the gene.

Reference Data

• RefSeq: NM_174985.1, NP_778150.1
• RefSeq Size: 990
• RefSeq ORF: 990
• Locus ID: 227289
• MW: 36.1 kDa