Galt (NM_001302511) Mouse Tagged ORF Clone

CAT#: MR228486

Galt (myc-DDK-tagged) - Mouse galactose-1-phosphate uridyl transferase (Galt), transcript variant 2

Specifications

Product Data

• Type: Mouse Tagged ORF Clone
• Tag: Myc-DDK
• Symbol: Galt
• Synonyms: AW553376
• Vector: pCMV6-Entry
• E. coli Selection: Kanamycin (25 ug/mL)
• Mammalian Cell Selection: Neomycin
• Sequence Data:
  ORF Nucleotide Sequence

  >MR228486 representing NM_001302511
  Red=Cloning site Blue=ORF Green=Tags(s)
  
  TTTTGTAATACGACTCACTATAGGGCGGCCGGGAATTCGTCGACTGGATCCGGTACCGAGGAGATCTGCC
  GCCGCGATCGCC
  
  ATGATGGGCTGTTCTAACCCCCATCCCCACTGCCAGGTTTGGGCTAGCAGCTTCCTGCCAGATATCGCCC
  AGCGTGAAGAGCGATCCCAGCAGACCTATCACAGCCAGCATGGAAAACCTTTGTTATTGGAATATGGTCA
  CCAAGAGCTCCTCAGGAAGGAACGTCTGGTCCTAACCAGTGAGCACTGGATAGTTCTGGTCCCCTTCTGG
  GCAGTGTGGCCTTTCCAGACACTTCTGCTGCCCCGGCGGCACGTGCGGCGGCTACCTGAGCTGAACCCCG
  CTGAGCGTGATGATCTCGCCTCCATCATGAAGAAGCTCTTGACCAAGTACGACAATCTATTTGAGACATC
  CTTTCCCTACTCCATGGGCTGGCATGGGGCTCCCACGGGATTAAAGACTGGAGCCACCTGTGACCACTGG
  CAGCTCCACGCCCACTACTACCCCCCACTTCTGCGATCCGCAACTGTCCGGAAGTTCATGGTTGGCTATG
  AAATGCTTGCCCAGGCCCAGCGTGACCTCACTCCCGAACAGGCCGCAGAAAGATTAAGGGCGCTTCCCGA
  GGTACACTATTGCCTGGCGCAGAAAGACAAGGAAACGGCAGCCATTGCT
  
  ACGCGTACGCGGCCGCTCGAGCAGAAACTCATCTCAGAAGAGGATCTGGCAGCAAATGATATCCTGGATT
  ACAAGGATGACGACGATAAGGTTTAA
  Protein Sequence

  >MR228486 representing NM_001302511
  Red=Cloning site Green=Tags(s)
  
  MMGCSNPHPHCQVWASSFLPDIAQREERSQQTYHSQHGKPLLLEYGHQELLRKERLVLTSEHWIVLVPFW
  AVWPFQTLLLPRRHVRRLPELNPAERDDLASIMKKLLTKYDNLFETSFPYSMGWHGAPTGLKTGATCDHW
  QLHAHYYPPLLRSATVRKFMVGYEMLAQAQRDLTPEQAAERLRALPEVHYCLAQKDKETAAIA
  
  TRTRPLEQKLISEEDLAANDILDYKDDDDKV
• Restriction Sites: SgfI-MluI Cloning Scheme for this gene Plasmid Map
• ACCN: NM_001302511
• ORF Size: 609 bp
• OTI Disclaimer: The molecular sequence of this clone aligns with the gene accession number as a point of reference only. However, individual transcript sequences of the same gene can differ through naturally occurring variations (e.g. polymorphisms), each with its own valid existence. This clone is substantially in agreement with the reference, but a complete review of all prevailing variants is recommended prior to use. More info
• OTI Annotation: This clone was engineered to express the complete ORF with an expression tag. Expression varies depending on the nature of the gene.

Reference Data

• RefSeq: NM_001302511.1, NP_001289440.1
• RefSeq Size: 1544
• RefSeq ORF: 612
• Locus ID: 14430
• MW: 24 kDa
• Gene Summary: The protein encoded by this gene is the second enzyme in the Leloir pathway, the metabolic pathway for D-galactose catabolism. It catalyzes the conversion of galactose-1-phosphate and uridine diphosphate-glucose to glucose-1-phosphate and uridine diphosphate galactose. Deficiency of this enzyme causes the genetic metabolic disorder galactosemia. Mice lacking this protein accumulate high levels of galactose and galactose-1 phosphate but are viable and fertile. This protein is negatively regulated through signaling by the polypeptide hormone prolactin, specifically via the short isoform of the prolactin receptor and the transcription factor Forkhead box O3. Alternative splicing results in multiple transcript variants. [provided by RefSeq, Oct 2014]