Cebpa (NM_001287521) Mouse Tagged ORF Clone

CAT#: MR228728

Cebpa (myc-DDK-tagged) - Mouse CCAAT/enhancer binding protein (C/EBP), alpha (Cebpa), transcript variant 1

Specifications

Product Data

• Type: Mouse Tagged ORF Clone
• Tag: Myc-DDK
• Symbol: Cebpa
• Synonyms: C/ebpalpha; CBF-A; Cebp
• Vector: pCMV6-Entry
• E. coli Selection: Kanamycin (25 ug/mL)
• Mammalian Cell Selection: Neomycin
• Sequence Data:
  ORF Nucleotide Sequence

  >MR228728 representing NM_001287521
  Red=Cloning site Blue=ORF Green=Tags(s)
  
  TTTTGTAATACGACTCACTATAGGGCGGCCGGGAATTCGTCGACTGGATCCGGTACCGAGGAGATCTGCC
  GCCGCGATCGCC
  
  ATGTCCGCGGGGGCGCACGGGCCCCCTCCCGGCTACGGCTGTGCGGCGGCCGGCTACCTGGACGGCAGGC
  TGGAGCCCCTGTACGAGCGCGTCGGGGCGCCCGCGCTACGGCCGCTGGTGATCAAACAAGAGCCCCGCGA
  GGAGGACGAGGCGAAGCAGCTGGCGCTGGCCGGCCTCTTCCCCTACCAGCCACCGCCGCCACCGCCACCG
  CCGCACCCGCACGCGTCTCCCGCGCACCTGGCCGCCCCCCACTTGCAGTTCCAGATCGCGCACTGCGGCC
  AGACCACCATGCACCTGCAGCCTGGCCACCCCACACCGCCGCCCACGCCCGTGCCCAGCCCGCACGCTGC
  GCCCGCCTTGGGTGCTGCGGGCCTGCCTGGCCCCGGGAGCGCGCTCAAGGGCTTGGCCGGTGCGCACCCC
  GACCTCCGCACGGGAGGCGGCGGCGGTGGCAGCGGTGCCGGTGCGGGCAAAGCCAAGAAGTCGGTGGACA
  AGAACAGCAACGAGTACCGGGTACGGCGGGAACGCAACAACATCGCGGTGCGCAAGAGCCGAGATAAAGC
  CAAACAACGCAACGTGGAGACGCAACAGAAGGTGCTGGAGTTGACCAGTGACAATGACCGCCTGCGCAAG
  CGGGTGGAACAGCTGAGCCGTGAACTGGACACGCTGCGGGGCATCTTCCGCCAGCTGCCTGAGAGCTCCT
  TGGTCAAGGCCATGGGCAACTGCGCG
  
  AGCGGACCGACGCGTACGCGGCCGCTCGAGCAGAAACTCATCTCAGAAGAGGATCTGGCAGCAAATGATATCC
  TGGATTACAAGGATGACGACGATAAGGTTTAA
  Protein Sequence

  >MR228728 representing NM_001287521
  Red=Cloning site Green=Tags(s)
  
  MSAGAHGPPPGYGCAAAGYLDGRLEPLYERVGAPALRPLVIKQEPREEDEAKQLALAGLFPYQPPPPPPP
  PHPHASPAHLAAPHLQFQIAHCGQTTMHLQPGHPTPPPTPVPSPHAAPALGAAGLPGPGSALKGLAGAHP
  DLRTGGGGGGSGAGAGKAKKSVDKNSNEYRVRRERNNIAVRKSRDKAKQRNVETQQKVLELTSDNDRLRK
  RVEQLSRELDTLRGIFRQLPESSLVKAMGNCA
  
  SGPTRTRPLEQKLISEEDLAANDILDYKDDDDKV
• Restriction Sites: SgfI-RsrII Cloning Scheme for this gene Plasmid Map
• ACCN: NM_001287521
• ORF Size: 726 bp
• OTI Disclaimer: The molecular sequence of this clone aligns with the gene accession number as a point of reference only. However, individual transcript sequences of the same gene can differ through naturally occurring variations (e.g. polymorphisms), each with its own valid existence. This clone is substantially in agreement with the reference, but a complete review of all prevailing variants is recommended prior to use. More info
• OTI Annotation: This clone was engineered to express the complete ORF with an expression tag. Expression varies depending on the nature of the gene.

Reference Data

• RefSeq: NM_001287521.1, NP_001274450.1
• RefSeq Size: 2636
• RefSeq ORF: 729
• Locus ID: 12606
• MW: 26 kDa
• Gene Summary: This intronless gene encodes a transcription factor that contains a basic leucine zipper (bZIP) domain and recognizes the CCAAT motif in the promoters of target genes. The encoded protein functions in homodimers and also heterodimers with CCAAT/enhancer-binding proteins beta and gamma. Activity of this protein can modulate the expression of genes involved in cell cycle regulation as well as in body weight homeostasis. The use of alternative in-frame non-AUG (CUG) and AUG start codons results in several protein isoforms with different lengths. Differential translation initiation is mediated by an out-of-frame, upstream open reading frame which is located between the CUG and the first AUG start codons. [provided by RefSeq, Sep 2014]