Cfd (NM_001291915) Mouse Tagged ORF Clone

CAT#: MR228840

Cfd (myc-DDK-tagged) - Mouse complement factor D (adipsin) (Cfd), transcript variant 2

Specifications

Product Data

• Type: Mouse Tagged ORF Clone
• Tag: Myc-DDK
• Symbol: Cfd
• Synonyms: Adn; DF
• Vector: pCMV6-Entry
• E. coli Selection: Kanamycin (25 ug/mL)
• Mammalian Cell Selection: Neomycin
• Sequence Data:
  ORF Nucleotide Sequence

  >MR228840 representing NM_001291915
  Red=Cloning site Blue=ORF Green=Tags(s)
  
  TTTTGTAATACGACTCACTATAGGGCGGCCGGGAATTCGTCGACTGGATCCGGTACCGAGGAGATCTGCC
  GCCGCGATCGCC
  
  ATGCACAGCTCCGTGTACTTCGTGGCTCTGGTGATCCTGGGAGCGGCTGTATGTGCACAGCCCCGAGGCC
  GGATTCTGGGTGGCCAGGAGGCCGCAGCCCATGCTCGGCCCTACATGGCTTCCGTGCAAGTGAACGGCAC
  ACACGTGTGCGGTGGCACCCTGCTGGACGAGCAGTGGGTGCTCAGTGCTGCACACTGCATGGATGGAGTG
  ACGGATGACGACTCTGTGCAGGTGCTCCTGGGTGCCCACTCCCTGTCCGCCCCTGAACCCTACAAGCGAT
  GGTATGATGTGCAGAGTGTAGTGCCTCACCCGGGCAGCCGACCTGACAGCCTTGAGGACGACCTCATTCT
  TTTTAAGCTATCCCAGAATGCCTCGTTGGGTCCCCACGTGAGACCCCTACCCTTGCAATACGAGGACAAA
  GAAGTGGAACCCGGCACGCTCTGCGACGTGGCTGGTTGGGGTGTGGTCACCCATGCAGGACGCAGGCCTG
  ATGTCCTGCATCAACTCAGAGTGTCAATCATGAACCGGACAACCTGCAATCTGCGCACGTACCATGACGG
  GGTAGTCACCATTAACATGATGTGTGCAGAGAGCAACCGCAGGGACACTTGCAGGGGAGACTCCGGCAGC
  CCTCTAGTGTGCGGGGATGCAGTCGAAGGTGTGGTTACGTGGGGCTCTCGCGTCTGTGGCAATGGCAAAA
  AGCCGGGCGTCTATACCCGAGTGTCATCCTACCGGATGTGGATCGAAAACATCACAAATGGTAACATGAC
  ATCC
  
  ACGCGTACGCGGCCGCTCGAGCAGAAACTCATCTCAGAAGAGGATCTGGCAGCAAATGATATCCTGGATT
  ACAAGGATGACGACGATAAGGTTTAA
  Protein Sequence

  >MR228840 representing NM_001291915
  Red=Cloning site Green=Tags(s)
  
  MHSSVYFVALVILGAAVCAQPRGRILGGQEAAAHARPYMASVQVNGTHVCGGTLLDEQWVLSAAHCMDGV
  TDDDSVQVLLGAHSLSAPEPYKRWYDVQSVVPHPGSRPDSLEDDLILFKLSQNASLGPHVRPLPLQYEDK
  EVEPGTLCDVAGWGVVTHAGRRPDVLHQLRVSIMNRTTCNLRTYHDGVVTINMMCAESNRRDTCRGDSGS
  PLVCGDAVEGVVTWGSRVCGNGKKPGVYTRVSSYRMWIENITNGNMTS
  
  TRTRPLEQKLISEEDLAANDILDYKDDDDKV
• Restriction Sites: SgfI-MluI Cloning Scheme for this gene Plasmid Map
• ACCN: NM_001291915
• ORF Size: 774 bp
• OTI Disclaimer: The molecular sequence of this clone aligns with the gene accession number as a point of reference only. However, individual transcript sequences of the same gene can differ through naturally occurring variations (e.g. polymorphisms), each with its own valid existence. This clone is substantially in agreement with the reference, but a complete review of all prevailing variants is recommended prior to use. More info
• OTI Annotation: This clone was engineered to express the complete ORF with an expression tag. Expression varies depending on the nature of the gene.

Reference Data

• RefSeq: NM_001291915.1, NM_001291915.2, NP_001278844.1
• RefSeq Size: 924
• RefSeq ORF: 777
• Locus ID: 11537
• MW: 28.4 kDa
• Gene Summary: This gene encodes a serine protease that plays an important role in the alternative pathway of complement activation for pathogen recognition and elimination. The encoded preproprotein undergoes proteolytic processing to generate a mature, functional enzyme that in turn cleaves factor B in the complement pathway. This gene is expressed in adipocytes and the mature enzyme is secreted into the bloodstream. Mice lacking the encoded product cannot initiate alternative pathway of complement activation. [provided by RefSeq, Jul 2016]