Spp1 (NM_001204201) Mouse Tagged ORF Clone

CAT#: MR229168

Spp1 (myc-DDK-tagged) - Mouse secreted phosphoprotein 1 (Spp1), transcript variant 1

Specifications

Product Data

• Type: Mouse Tagged ORF Clone
• Tag: Myc-DDK
• Symbol: Spp1
• Synonyms: 2AR; Apl-1; BNSP; Bsp; BSPI; Eta; ETA-1; OP; Opn; Opnl; Ric; Spp-1
• Vector: pCMV6-Entry
• E. coli Selection: Kanamycin (25 ug/mL)
• Mammalian Cell Selection: Neomycin
• Sequence Data:
  ORF Nucleotide Sequence

  >MR229168 representing NM_001204201
  Red=Cloning site Blue=ORF Green=Tags(s)
  
  TTTTGTAATACGACTCACTATAGGGCGGCCGGGAATTCGTCGACTGGATCCGGTACCGAGGAGATCTGCC
  GCCGCGATCGCC
  
  ATGAGGCTGCAGTTCTCCTGGCTGAATTCTGAGGGACTAACTACGACCATGAGATTGGCAGTGATTTGCT
  TTTGCCTGTTTGGCATTGCCTCCTCCCTCCCGGTGAAAGTGACTGATTCTGGCAGCTCAGAGGAGAAGAA
  GCTTTACAGCCTGCACCCAGATCCTATAGCCACATGGCTGGTGCCTGACCCATCTCAGAAGCAGAATCTC
  CTTGCGCCACAGAATGCTGTGTCCTCTGAAGAAAAGGATGACTTTAAGCAAGAAACTCTTCCAAGCAATT
  CCAATGAAAGCCATGACCACATGGACGACGATGATGACGATGATGATGACGATGGAGACCATGCAGAGAG
  CGAGGATTCTGTGGACTCGGATGAATCTGACGAATCTCACCATTCGGATGAGTCTGATGAGACCGTCACT
  GCTAGTACACAAGCAGACACTTTCACTCCAATCGTCCCTACAGTCGATGTCCCCAACGGCCGAGGTGATA
  GCTTGGCTTATGGACTGAGGTCAAAGTCTAGGAGTTTCCAGGTTTCTGATGAACAGTATCCTGATGCCAC
  AGATGAGGACCTCACCTCTCACATGAAGAGCGGTGAGTCTAAGGAGTCCCTCGATGTCATCCCTGTTGCC
  CAGCTTCTGAGCATGCCCTCTGATCAGGACAACAACGGAAAGGGCAGCCATGAGTCAAGTCAGCTGGATG
  AACCAAGTCTGGAAACACACAGACTTGAGCATTCCAAAGAGAGCCAGGAGAGTGCCGATCAGTCGGATGT
  GATCGATAGTCAAGCAAGTTCCAAAGCCAGCCTGGAACATCAGAGCCACAAGTTTCACAGCCACAAGGAC
  AAGCTAGTCCTAGACCCTAAGAGTAAGGAAGATGATAGGTATCTGAAATTCCGAATTTCTCATGAATTAG
  AGAGTTCATCTTCTGAGGTCAAC
  
  ACGCGTACGCGGCCGCTCGAGCAGAAACTCATCTCAGAAGAGGATCTGGCAGCAAATGATATCCTGGATT
  ACAAGGATGACGACGATAAGGTTTAA
  Protein Sequence

  >MR229168 representing NM_001204201
  Red=Cloning site Green=Tags(s)
  
  MRLQFSWLNSEGLTTTMRLAVICFCLFGIASSLPVKVTDSGSSEEKKLYSLHPDPIATWLVPDPSQKQNL
  LAPQNAVSSEEKDDFKQETLPSNSNESHDHMDDDDDDDDDDGDHAESEDSVDSDESDESHHSDESDETVT
  ASTQADTFTPIVPTVDVPNGRGDSLAYGLRSKSRSFQVSDEQYPDATDEDLTSHMKSGESKESLDVIPVA
  QLLSMPSDQDNNGKGSHESSQLDEPSLETHRLEHSKESQESADQSDVIDSQASSKASLEHQSHKFHSHKD
  KLVLDPKSKEDDRYLKFRISHELESSSSEVN
  
  TRTRPLEQKLISEEDLAANDILDYKDDDDKV
• Restriction Sites: SgfI-MluI Cloning Scheme for this gene Plasmid Map
• ACCN: NM_001204201
• ORF Size: 933 bp
• OTI Disclaimer: Due to the inherent nature of this plasmid, standard methods to replicate additional amounts of DNA in E. coli are highly likely to result in mutations and/or rearrangements. Therefore, OriGene does not guarantee the capability to replicate this plasmid DNA. Additional amounts of DNA can be purchased from OriGene with batch-specific, full-sequence verification at a reduced cost. Please contact our customer care team at custsupport@origene.com or by calling 301.340.3188 option 3 for pricing and delivery.
  
  The molecular sequence of this clone aligns with the gene accession number as a point of reference only. However, individual transcript sequences of the same gene can differ through naturally occurring variations (e.g. polymorphisms), each with its own valid existence. This clone is substantially in agreement with the reference, but a complete review of all prevailing variants is recommended prior to use. More info
• OTI Annotation: This clone was engineered to express the complete ORF with an expression tag. Expression varies depending on the nature of the gene.

Reference Data

• RefSeq: NM_001204201.1, NP_001191130.1
• RefSeq Size: 1475
• RefSeq ORF: 936
• Locus ID: 20750
• MW: 34.9 kDa
• Gene Summary: Acts as a cytokine involved in enhancing production of interferon-gamma and interleukin-12 and reducing production of interleukin-10 and is essential in the pathway that leads to type I immunity. [UniProtKB/Swiss-Prot Function]