Gpr146 (NM_030258) Mouse Tagged ORF Clone

CAT#: MR229298

Gpr146 (myc-DDK-tagged) - Mouse G protein-coupled receptor 146 (Gpr146), transcript variant 1

Specifications

Product Data

• Type: Mouse Tagged ORF Clone
• Tag: Myc-DDK
• Symbol: Gpr146
• Synonyms: BC003323; D830046C22Rik; PGR8
• Vector: pCMV6-Entry
• E. coli Selection: Kanamycin (25 ug/mL)
• Mammalian Cell Selection: Neomycin
• Sequence Data:
  ORF Nucleotide Sequence

  >MR229298 representing NM_030258
  Red=Cloning site Blue=ORF Green=Tags(s)
  
  TTTTGTAATACGACTCACTATAGGGCGGCCGGGAATTCGTCGACTGGATCCGGTACCGAGGAGATCTGCC
  GCCGCGATCGCC
  
  ATGTGGAGCTGTGGCCCACTCAACAGCACAGCGTGGGCTGAGGAGCCGCTGTGCCGGAACCTGCGCCTGG
  GGCTGTGGGTCCTCTCGCTGCTCTACCTGGGGGCAGGGGTCCCTGTGAGCTTAGGCTATAATGCTCTTCT
  GGTGCTGGCCAACCTGGCCAGCAAGAACACCATGACCATGCCCGACGTGTACTTCGTGAACATGGCCGTG
  GCGGGGCTGGTGCTCACGGCACTGGCACCTGCGTACCTGTTGGGCCCTGCCCACTCCAGGTGGGCCCTGT
  GGAGCCTCAGCAGTGAGGCCCATGTGACACTGCTCATCCTGTTCAACGTGGCTTCCCTGGTGACCATGTA
  CTCCACTGCACTGCTGAGCCTTGACTACTACATCGAGCGTGCCCTGCCACGCACCTACATGGCCAGTGTG
  TACAACACCCGGCACGTGTGTGGCTTCGTCTGGGGAGGGGCGGTGCTCACCAGCTTCTCCTCCCTGCTCT
  TCTACATCTGCAGTCACGTGTCTTCTAGAATCGCTGAGTGTGCCCGGATGCAGAACACGGAGGCAGCCGA
  TGCTATCCTTGTGCTCATCGGCTACGTGGTGCCAGGTCTGGCTGTGTTGTATGCCCTGGCACTCATCTCG
  AGAATCGGGAAGGAAGACACACCCCTGGACCAGGACACCAGCAGGCTGGACCCCTCGGTGCACAGGCTGC
  TGGTGGCCACCGTGTGCACTCAGTTTGGCCTCTGGACACCTTACTACTTGAGCCTGGGGCACACAGTGCT
  GACGTCACGGGGGAGGACCGTGGAGGGGCATTATCTGGGCATCCTACAGGTTGCTAAGGACCTGGCCAAG
  TTCCTGGCCTTCTCAAGCAGTTCTGTGACACCACTGCTCTACCGTTACATCAACAAAGCCTTCCCCGGCA
  AGCTCCGGCGGCTGATGAAGAAGATGCACTGCGGGCGCCGCCACTGCTCCCCCGACCCCTCGGGGATACA
  GCAGGTGATGGCACAGGCG
  
  ACGCGTACGCGGCCGCTCGAGCAGAAACTCATCTCAGAAGAGGATCTGGCAGCAAATGATATCCTGGATT
  ACAAGGATGACGACGATAAGGTTTAA
  Protein Sequence

  >MR229298 representing NM_030258
  Red=Cloning site Green=Tags(s)
  
  MWSCGPLNSTAWAEEPLCRNLRLGLWVLSLLYLGAGVPVSLGYNALLVLANLASKNTMTMPDVYFVNMAV
  AGLVLTALAPAYLLGPAHSRWALWSLSSEAHVTLLILFNVASLVTMYSTALLSLDYYIERALPRTYMASV
  YNTRHVCGFVWGGAVLTSFSSLLFYICSHVSSRIAECARMQNTEAADAILVLIGYVVPGLAVLYALALIS
  RIGKEDTPLDQDTSRLDPSVHRLLVATVCTQFGLWTPYYLSLGHTVLTSRGRTVEGHYLGILQVAKDLAK
  FLAFSSSSVTPLLYRYINKAFPGKLRRLMKKMHCGRRHCSPDPSGIQQVMAQA
  
  TRTRPLEQKLISEEDLAANDILDYKDDDDKV
• Restriction Sites: SgfI-MluI Cloning Scheme for this gene Plasmid Map
• ACCN: NM_030258
• ORF Size: 999 bp
• OTI Disclaimer: The molecular sequence of this clone aligns with the gene accession number as a point of reference only. However, individual transcript sequences of the same gene can differ through naturally occurring variations (e.g. polymorphisms), each with its own valid existence. This clone is substantially in agreement with the reference, but a complete review of all prevailing variants is recommended prior to use. More info
• OTI Annotation: This clone was engineered to express the complete ORF with an expression tag. Expression varies depending on the nature of the gene.
• Product Components: The ORF clone is ion-exchange column purified, transfection-ready dried plasmid DNA, and shipped with 2 vector sequencing primers.
• Reconstitution: 1. Centrifuge at 5,000xg for 5min.
  2. Carefully open the tube and add 100ul of sterile water to dissolve the DNA.
  3. Close the tube and incubate for 10 minutes at room temperature.
  4. Briefly vortex the tube and then do a quick spin (less than 5000xg) to concentrate the liquid at the bottom.
  5. Store the suspended plasmid at -20°C. The DNA is stable for at least one year from date of shipping when stored at -20°C.

Reference Data

• RefSeq: NM_030258.1, NM_030258.2, NM_030258.3, NM_030258.4, NP_084534.2
• RefSeq Size: 4065 bp
• RefSeq ORF: 1002 bp
• Locus ID: 80290
• MW: 36.6 kDa
• Gene Summary: Orphan receptor. [UniProtKB/Swiss-Prot Function]