Nelfe (NM_001045864) Mouse Tagged ORF Clone

CAT#: MR229518

Nelfe (myc-DDK-tagged) - Mouse negative elongation factor complex member E, Rdbp (Nelfe), transcript variant 3

Specifications

Product Data

• Type: Mouse Tagged ORF Clone
• Tag: Myc-DDK
• Symbol: Nelfe
• Synonyms: D17H6S45; NELF-E; RD; Rdbp
• Vector: pCMV6-Entry
• E. coli Selection: Kanamycin (25 ug/mL)
• Mammalian Cell Selection: Neomycin
• Sequence Data:
  ORF Nucleotide Sequence

  >MR229518 representing NM_001045864
  Red=Cloning site Blue=ORF Green=Tags(s)
  
  TTTTGTAATACGACTCACTATAGGGCGGCCGGGAATTCGTCGACTGGATCCGGTACCGAGGAGATCTGCC
  GCCGCGATCGCC
  
  ATGTTGGTGATACCTCCGGGGCTGAGTGAAGAGGAAGAGGCACTGCAGAAGAAGTTCAACAAACTCAAGA
  AGAAGAAAAAGGCATTGCTGGCTCTGAAGAAGCAGAGCAGCAGCGGCCCAGCGAGCCAGGGCGGTGTCAA
  ACGCTCACTGTCAGAGCAGCCTGTGGTGGACACTGCCACAGCAACAGAACAGGCCAAACAACTGGTGAAG
  TCAGGAGCCATCAGTGCCATCAAGGCTGAAACTAAGAACTCAGGCTTCAAACGTTCTCGGACCCTGGAAG
  GGAAGTTAAAGGACCCAGAGAAGGGGCCTGTCCCCACTTTCCAGCCGTTCCAGAGGAGCATGTCTGCTGA
  TGAGGACCTACAGGAGCCATCCAGACGTCCCCAGAGGAAATCTCTGTATGAAAGCTTTGTGTCTTCTAGT
  GATCGGCTTCGGGAACTGGGACAAGATGGAGAAGAAGCAGAGGCCCCCGGGGCTGGTGATGGCCCACCTC
  GAGGCTTTGACTGGAGCTATGAAGAGCATGGTAGCGCCCGCTCCTCAGCCTCCCCTCCCCGAAGCCGCAG
  CAGGGACCGAAGTCATGACCGGAGCCGAGATAGGGATCGGGACAAAGAGAGAGACAGAGACCGAGATCGA
  GATCGAGACAGAGACCGGGATAAAGACAAAGACAGAGACCGGGATCGAGACAGAGACCGGGACCGAGATC
  GGGAACGAGACCGAGAGGGCCCTTTCCGCAGGTCAGACTCATTCCCCGAACGGAGGGCCCCTCGAAAGGG
  GAACACACTGTATGTGTATGGAGAGGACATGACGCCCACCCTCCTCCGCGGGGCCTTCTCTCCCTTTGGA
  AATATCATCGACCTCTCCATGGACCCACCCAGAAACTGTGCCTTCGTCACCTACGAGAAGATGGAGTCTG
  CAGATCAGGCTGTTGCTGAGCTCAATGGGACCCAGGTGGAATCTGTGCAGCTTAAAGTCAACATAGCCCG
  AAAACAGCCCATGCTGGACGCTGCTACTGGCAAATCTGTCTGGGGCTCCCTTGCTGTCCAGAACAGCCCT
  AAGGGTTGCCATCGAGACAAGAGGACCCAGATTGTGTACAGTGACGATCTA
  
  ACGCGTACGCGGCCGCTCGAGCAGAAACTCATCTCAGAAGAGGATCTGGCAGCAAATGATATCCTGGATT
  ACAAGGATGACGACGATAAGGTTTAA
  Protein Sequence

  >MR229518 representing NM_001045864
  Red=Cloning site Green=Tags(s)
  
  MLVIPPGLSEEEEALQKKFNKLKKKKKALLALKKQSSSGPASQGGVKRSLSEQPVVDTATATEQAKQLVK
  SGAISAIKAETKNSGFKRSRTLEGKLKDPEKGPVPTFQPFQRSMSADEDLQEPSRRPQRKSLYESFVSSS
  DRLRELGQDGEEAEAPGAGDGPPRGFDWSYEEHGSARSSASPPRSRSRDRSHDRSRDRDRDKERDRDRDR
  DRDRDRDKDKDRDRDRDRDRDRDRERDREGPFRRSDSFPERRAPRKGNTLYVYGEDMTPTLLRGAFSPFG
  NIIDLSMDPPRNCAFVTYEKMESADQAVAELNGTQVESVQLKVNIARKQPMLDAATGKSVWGSLAVQNSP
  KGCHRDKRTQIVYSDDL
  
  TRTRPLEQKLISEEDLAANDILDYKDDDDKV
• Restriction Sites: SgfI-MluI Cloning Scheme for this gene Plasmid Map
• ACCN: NM_001045864
• ORF Size: 1101 bp
• OTI Disclaimer: The molecular sequence of this clone aligns with the gene accession number as a point of reference only. However, individual transcript sequences of the same gene can differ through naturally occurring variations (e.g. polymorphisms), each with its own valid existence. This clone is substantially in agreement with the reference, but a complete review of all prevailing variants is recommended prior to use. More info
• OTI Annotation: This clone was engineered to express the complete ORF with an expression tag. Expression varies depending on the nature of the gene.

Reference Data

• RefSeq: NM_001045864.1, NP_001039329.1
• RefSeq Size: 1621
• RefSeq ORF: 1104
• Locus ID: 27632
• MW: 41.5 kDa