Acta1 (NM_001272041) Mouse Tagged ORF Clone
CAT#: MR229578
- TrueORF®
Acta1 (myc-DDK-tagged) - Mouse actin, alpha 1, skeletal muscle (Acta1), transcript variant 1
"NM_001272041" in other vectors (1)
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Product Images
![](https://cdn.origene.com/img/defaults-img-expression-plasmids.jpg)
Specifications
Product Data | |
Type | Mouse Tagged ORF Clone |
Tag | Myc-DDK |
Symbol | Acta1 |
Synonyms | AA959943; Acta-2; Acts; Actsk-1 |
Vector | pCMV6-Entry |
E. coli Selection | Kanamycin (25 ug/mL) |
Mammalian Cell Selection | Neomycin |
Sequence Data |
>MR229578 representing NM_001272041
Red=Cloning site Blue=ORF Green=Tags(s) TTTTGTAATACGACTCACTATAGGGCGGCCGGGAATTCGTCGACTGGATCCGGTACCGAGGAGATCTGCC GCCGCGATCGCC ATGTGCGACGAAGACGAGACCACCGCTCTTGTGTGTGACAACGGCTCTGGCCTGGTGAAAGCTGGCTTTG CCGGGGATGATGCCCCCAGGGCTGTGTTCCCATCCATCGTGGGCCGACCCCGTCACCAGGGTGTCATGGT AGGTATGGGTCAGAAGGACTCCTACGTGGGTGATGAGGCCCAGAGCAAGCGAGGTATCCTGACCCTGAAG TACCCCATTGAACATGGCATCATCACCAACTGGGACGACATGGAGAAGATCTGGCACCACACCTTCTACA ATGAGCTGCGTGTGGCCCCTGAGGAGCACCCGACTCTGCTCACCGAGGCCCCCCTGAACCCCAAAGCTAA CCGGGAGAAGATGACTCAAATCATGTTTGAGACCTTCAACGTGCCTGCCATGTATGTGGCTATCCAGGCG GTGCTGTCCCTCTATGCCTCCGGCCGTACCACCGGCATCGTGTTGGATTCTGGGGACGGTGTCACCCACA ACGTGCCCATCTATGAGGGCTATGCCCTGCCACACGCCATCATGCGTCTGGACCTGGCCGGTCGCGACCT CACTGACTACCTGATGAAAATCCTCACTGAGCGTGGCTATTCCTTCGTGACCACAGCTGAACGTGAGATT GTGCGCGACATCAAAGAGAAGCTGTGCTATGTGGCCCTGGACTTCGAGAATGAGATGGCCACCGCTGCCT CTTCCTCCTCCCTGGAGAAGAGCTATGAGCTGCCTGACGGGCAGGTCATCACCATCGGCAATGAGCGTTT CCGTTGCCCGGAGACGCTCTTCCAGCCTTCCTTTATCGGTATGGAGTCTGCGGGGATCCATGAGACCACC TACAACAGCATCATGAAGTGCGACATCGACATCAGGAAGGACCTGTACGCCAACAACGTCATGTCAGGGG GCACCACCATGTACCCTGGTATCGCTGACCGCATGCAGAAGGAGATCACAGCTCTGGCTCCCAGCACCAT GAAGATCAAGATCATCGCCCCCCCTGAGCGCAAGTACTCAGTGTGGATCGGTGGCTCCATCCTGGCCTCG CTGTCCACCTTCCAGCAGATGTGGATCACCAAGCAGGAGTACGACGAGGCTGGCCCCTCCATTGTGCACC GCAAATGCTTC ACGCGTACGCGGCCGCTCGAGCAGAAACTCATCTCAGAAGAGGATCTGGCAGCAAATGATATCCTGGATT ACAAGGATGACGACGATAAGGTTTAA >MR229578 representing NM_001272041
Red=Cloning site Green=Tags(s) MCDEDETTALVCDNGSGLVKAGFAGDDAPRAVFPSIVGRPRHQGVMVGMGQKDSYVGDEAQSKRGILTLK YPIEHGIITNWDDMEKIWHHTFYNELRVAPEEHPTLLTEAPLNPKANREKMTQIMFETFNVPAMYVAIQA VLSLYASGRTTGIVLDSGDGVTHNVPIYEGYALPHAIMRLDLAGRDLTDYLMKILTERGYSFVTTAEREI VRDIKEKLCYVALDFENEMATAASSSSLEKSYELPDGQVITIGNERFRCPETLFQPSFIGMESAGIHETT YNSIMKCDIDIRKDLYANNVMSGGTTMYPGIADRMQKEITALAPSTMKIKIIAPPERKYSVWIGGSILAS LSTFQQMWITKQEYDEAGPSIVHRKCF TRTRPLEQKLISEEDLAANDILDYKDDDDKV |
Restriction Sites |
SgfI-MluI
Cloning Scheme for this gene
Plasmid Map
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ACCN | NM_001272041 |
ORF Size | 1131 bp |
OTI Disclaimer | The molecular sequence of this clone aligns with the gene accession number as a point of reference only. However, individual transcript sequences of the same gene can differ through naturally occurring variations (e.g. polymorphisms), each with its own valid existence. This clone is substantially in agreement with the reference, but a complete review of all prevailing variants is recommended prior to use. More info |
OTI Annotation | This clone was engineered to express the complete ORF with an expression tag. Expression varies depending on the nature of the gene. |
Product Components | The ORF clone is ion-exchange column purified, transfection-ready dried plasmid DNA, and shipped with 2 vector sequencing primers. |
Reconstitution | 1. Centrifuge at 5,000xg for 5min. 2. Carefully open the tube and add 100ul of sterile water to dissolve the DNA. 3. Close the tube and incubate for 10 minutes at room temperature. 4. Briefly vortex the tube and then do a quick spin (less than 5000xg) to concentrate the liquid at the bottom. 5. Store the suspended plasmid at -20°C. The DNA is stable for at least one year from date of shipping when stored at -20°C. |
Reference Data | |
RefSeq | NM_001272041.1, NP_001258970.1 |
RefSeq Size | 1571 bp |
RefSeq ORF | 1134 bp |
Locus ID | 11459 |
MW | 42.1 kDa |
Gene Summary | Actins are highly conserved proteins that are involved in various types of cell motility and are ubiquitously expressed in all eukaryotic cells. [UniProtKB/Swiss-Prot Function] |
Documents
Product Manuals |
FAQs |
Resources
Other Versions
SKU | Description | Size | Price |
---|---|---|---|
MC227367 | Acta1 (untagged) - Mouse actin, alpha 1, skeletal muscle (Acta1), transcript variant 1 |
USD 380.00 |
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