Crhr2 (NM_001288619) Mouse Tagged ORF Clone

CAT#: MR229768

Crhr2 (myc-DDK-tagged) - Mouse corticotropin releasing hormone receptor 2 (Crhr2), transcript variant 3

Specifications

Product Data

• Type: Mouse Tagged ORF Clone
• Tag: Myc-DDK
• Symbol: Crhr2
• Synonyms: CRF-R2; Crfr2; CRFR2alpha; CRFR2beta
• Vector: pCMV6-Entry
• E. coli Selection: Kanamycin (25 ug/mL)
• Mammalian Cell Selection: Neomycin
• Sequence Data:
  ORF Nucleotide Sequence

  >MR229768 representing NM_001288619
  Red=Cloning site Blue=ORF Green=Tags(s)
  
  TTTTGTAATACGACTCACTATAGGGCGGCCGGGAATTCGTCGACTGGATCCGGTACCGAGGAGATCTGCC
  GCCGCGATCGCC
  
  ATGGACGCGGCGCTGCTCCTCAGCCTGCTGGAGGCCAACTGCAGCCTGGCGCTGGCCGAAGAGCTGCTCC
  TGGACGGCTGGGGAGTGCCCCCGGACCCCGAAGGTCCCTACACCTACTGCAACACGACCTTGGACCAGAT
  CGGGACCTGCTGGCCACAGAGCGCACCCGGAGCCCTAGTAGAGAGACCGTGCCCCGAGTACTTCAATGGC
  ATCAAGTACAACACGACCCGGAATGCCTACAGAGAGTGCCTGGAGAACGGGACCTGGGCCTCAAGGGTCA
  ACTACTCACACTGCGAACCCATTTTGGATGACAAGCAGAGAAAGTATGACCTGCATTACCGAATCGCCCT
  CATTGTCAACTACCTGGGTCACTGTGTTTCCGTGGTGGCCCTGGTGGCCGCTTTCCTGCTTTTCCTAGTG
  CTGCGGAGTATCCGCTGCCTGAGGAATGTGATCCACTGGAACCTCATCACCACCTTCATTCTGAGAAACA
  TCGCGTGGTTCCTGCTGCAACTCATCGACCACGAAGTGCACGAGGGCAATGAGGTCTGGTGCCGCTGCAT
  CACCACCATCTTCAACTATTTTGTGGTCACCAACTTCTTCTGGATGTTTGTGGAGGGCTGCTACCTGCAC
  ACGGCCATTGTCATGACGTACTCCACAGAGCACCTGCGCAAGTGGCTTTTCCTCTTCATTGGATGGTGCA
  TTCCCTGCCCTATCATCATCGCCTGGGCAGTTGGCAAACTCTACTATGAGAATGAGCAGTGCTGGTTTGG
  CAAGGAAGCTGGTGATTTGGTGGACTACATCTACCAGGGCCCCGTCATGCTTGTGCTGTTGATCAATTTT
  GTATTTCTGTTTAACATCGTCAGGATCCTGATGACGAAGTTACGAGCATCCACCACGTCCGAGACAATCC
  AATACAGGAAGGCAGTGAAGGCCACGCTGGTCCTCCTCCCCCTGTTGGGCATCACCTACATGCTCTTCTT
  TGTCAATCCTGGCGAGGACGACCTGTCCCAGATTGTGTTCATCTACTTCAACTCTTTCCTGCAGTCCTTC
  CAGGGTTTCTTTGTGTCCGTTTTCTACTGCTTCTTCAATGGAGAGGTGCGCGCGGCCCTGAGAAAGCGGT
  GGCACCGCTGGCAGGACCACCACGCCCTCCGGGTGCCTGTGGCCCGGGCCATGTCCATCCCTACGTCGCC
  CACCAGGATCAGCTTCCACAGCATCAAGCAGACAGCTGCTGTG
  
  ACGCGTACGCGGCCGCTCGAGCAGAAACTCATCTCAGAAGAGGATCTGGCAGCAAATGATATCCTGGATT
  ACAAGGATGACGACGATAAGGTTTAA
  Protein Sequence

  >MR229768 representing NM_001288619
  Red=Cloning site Green=Tags(s)
  
  MDAALLLSLLEANCSLALAEELLLDGWGVPPDPEGPYTYCNTTLDQIGTCWPQSAPGALVERPCPEYFNG
  IKYNTTRNAYRECLENGTWASRVNYSHCEPILDDKQRKYDLHYRIALIVNYLGHCVSVVALVAAFLLFLV
  LRSIRCLRNVIHWNLITTFILRNIAWFLLQLIDHEVHEGNEVWCRCITTIFNYFVVTNFFWMFVEGCYLH
  TAIVMTYSTEHLRKWLFLFIGWCIPCPIIIAWAVGKLYYENEQCWFGKEAGDLVDYIYQGPVMLVLLINF
  VFLFNIVRILMTKLRASTTSETIQYRKAVKATLVLLPLLGITYMLFFVNPGEDDLSQIVFIYFNSFLQSF
  QGFFVSVFYCFFNGEVRAALRKRWHRWQDHHALRVPVARAMSIPTSPTRISFHSIKQTAAV
  
  TRTRPLEQKLISEEDLAANDILDYKDDDDKV
• Restriction Sites: SgfI-MluI Cloning Scheme for this gene Plasmid Map
• ACCN: NM_001288619
• ORF Size: 1233 bp
• OTI Disclaimer: The molecular sequence of this clone aligns with the gene accession number as a point of reference only. However, individual transcript sequences of the same gene can differ through naturally occurring variations (e.g. polymorphisms), each with its own valid existence. This clone is substantially in agreement with the reference, but a complete review of all prevailing variants is recommended prior to use. More info
• OTI Annotation: This clone was engineered to express the complete ORF with an expression tag. Expression varies depending on the nature of the gene.

Reference Data

• RefSeq: NM_001288619.1, NP_001275548.1
• RefSeq Size: 2607
• RefSeq ORF: 1236
• Locus ID: 12922
• MW: 48.1 kDa
• Gene Summary: G-protein coupled receptor for CRH (corticotropin-releasing factor), UCN (urocortin), UCN2 and UCN3. Has high affinity for UCN. Ligand binding causes a conformation change that triggers signaling via guanine nucleotide-binding proteins (G proteins) and down-stream effectors, such as adenylate cyclase. Promotes the activation of adenylate cyclase, leading to increased intracellular cAMP levels. [UniProtKB/Swiss-Prot Function]