Crhr2 (NM_001288618) Mouse Tagged ORF Clone

CAT#: MR229875

Crhr2 (myc-DDK-tagged) - Mouse corticotropin releasing hormone receptor 2 (Crhr2), transcript variant 1

Specifications

Product Data

• Type: Mouse Tagged ORF Clone
• Tag: Myc-DDK
• Symbol: Crhr2
• Synonyms: CRF-R2; Crfr2; CRFR2alpha; CRFR2beta
• Vector: pCMV6-Entry
• E. coli Selection: Kanamycin (25 ug/mL)
• Mammalian Cell Selection: Neomycin
• Sequence Data:
  ORF Nucleotide Sequence

  >MR229875 representing NM_001288618
  Red=Cloning site Blue=ORF Green=Tags(s)
  
  TTTTGTAATACGACTCACTATAGGGCGGCCGGGAATTCGTCGACTGGATCCGGTACCGAGGAGATCTGCC
  GCCGCGATCGCC
  
  ATGGGGACCCCAGGCTCTCTTCCCAGTGCACAGCTTCTCCTCTGCCTGTTTTCCCTGCTTCCAGTGCTCC
  AGGTGGCCCAACCAGGCCAGGCACCCCAGGACCAGCCCCTGTGGACACTTTTGGAGCAGTACTGCCACAG
  GACCACAACTGGGAATTTTTCAGGTCCCTACACCTACTGCAACACGACCTTGGACCAGATCGGGACCTGC
  TGGCCACAGAGCGCACCCGGAGCCCTAGTAGAGAGACCGTGCCCCGAGTACTTCAATGGCATCAAGTACA
  ACACGACCCGGAATGCCTACAGAGAGTGCCTGGAGAACGGGACCTGGGCCTCAAGGGTCAACTACTCACA
  CTGCGAACCCATTTTGGATGACAAGCAGAGAAAGTATGACCTGCATTACCGAATCGCCCTCATTGTCAAC
  TACCTGGGTCACTGTGTTTCCGTGGTGGCCCTGGTGGCCGCTTTCCTGCTTTTCCTAGTGCTGCGGAGTA
  TCCGCTGCCTGAGGAATGTGATCCACTGGAACCTCATCACCACCTTCATTCTGAGAAACATCGCGTGGTT
  CCTGCTGCAACTCATCGACCACGAAGTGCACGAGGGCAATGAGGTCTGGTGCCGCTGCATCACCACCATC
  TTCAACTATTTTGTGGTCACCAACTTCTTCTGGATGTTTGTGGAGGGCTGCTACCTGCACACGGCCATTG
  TCATGACGTACTCCACAGAGCACCTGCGCAAGTGGCTTTTCCTCTTCATTGGATGGTGCATTCCCTGCCC
  TATCATCATCGCCTGGGCAGTTGGCAAACTCTACTATGAGAATGAGCAGTGCTGGTTTGGCAAGGAAGCT
  GGTGATTTGGTGGACTACATCTACCAGGGCCCCGTCATGCTTGTGCTGTTGATCAATTTTGTATTTCTGT
  TTAACATCGTCAGGATCCTGATGACGAAGTTACGAGCATCCACCACGTCCGAGACAATCCAATACAGGAA
  GGCAGTGAAGGCCACGCTGGTCCTCCTCCCCCTGTTGGGCATCACCTACATGCTCTTCTTTGTCAATCCT
  GGCGAGGACGACCTGTCCCAGATTGTGTTCATCTACTTCAACTCTTTCCTGCAGTCCTTCCAGGGTTTCT
  TTGTGTCCGTTTTCTACTGCTTCTTCAATGGAGAGGTGCGCGCGGCCCTGAGAAAGCGGTGGCACCGCTG
  GCAGGACCACCACGCCCTCCGGGTGCCTGTGGCCCGGGCCATGTCCATCCCTACGTCGCCCACCAGGATC
  AGCTTCCACAGCATCAAGCAGACAGCTGCTGTG
  
  ACGCGTACGCGGCCGCTCGAGCAGAAACTCATCTCAGAAGAGGATCTGGCAGCAAATGATATCCTGGATT
  ACAAGGATGACGACGATAAGGTTTAA
  Protein Sequence

  >MR229875 representing NM_001288618
  Red=Cloning site Green=Tags(s)
  
  MGTPGSLPSAQLLLCLFSLLPVLQVAQPGQAPQDQPLWTLLEQYCHRTTTGNFSGPYTYCNTTLDQIGTC
  WPQSAPGALVERPCPEYFNGIKYNTTRNAYRECLENGTWASRVNYSHCEPILDDKQRKYDLHYRIALIVN
  YLGHCVSVVALVAAFLLFLVLRSIRCLRNVIHWNLITTFILRNIAWFLLQLIDHEVHEGNEVWCRCITTI
  FNYFVVTNFFWMFVEGCYLHTAIVMTYSTEHLRKWLFLFIGWCIPCPIIIAWAVGKLYYENEQCWFGKEA
  GDLVDYIYQGPVMLVLLINFVFLFNIVRILMTKLRASTTSETIQYRKAVKATLVLLPLLGITYMLFFVNP
  GEDDLSQIVFIYFNSFLQSFQGFFVSVFYCFFNGEVRAALRKRWHRWQDHHALRVPVARAMSIPTSPTRI
  SFHSIKQTAAV
  
  TRTRPLEQKLISEEDLAANDILDYKDDDDKV
• Restriction Sites: SgfI-MluI Cloning Scheme for this gene Plasmid Map
• ACCN: NM_001288618
• ORF Size: 1293 bp
• OTI Disclaimer: The molecular sequence of this clone aligns with the gene accession number as a point of reference only. However, individual transcript sequences of the same gene can differ through naturally occurring variations (e.g. polymorphisms), each with its own valid existence. This clone is substantially in agreement with the reference, but a complete review of all prevailing variants is recommended prior to use. More info
• OTI Annotation: This clone was engineered to express the complete ORF with an expression tag. Expression varies depending on the nature of the gene.

Reference Data

• RefSeq: NM_001288618.1, NP_001275547.1
• RefSeq Size: 2700
• RefSeq ORF: 1296
• Locus ID: 12922
• MW: 50.4 kDa
• Gene Summary: G-protein coupled receptor for CRH (corticotropin-releasing factor), UCN (urocortin), UCN2 and UCN3. Has high affinity for UCN. Ligand binding causes a conformation change that triggers signaling via guanine nucleotide-binding proteins (G proteins) and down-stream effectors, such as adenylate cyclase. Promotes the activation of adenylate cyclase, leading to increased intracellular cAMP levels. [UniProtKB/Swiss-Prot Function]