Cast (NM_001301181) Mouse Tagged ORF Clone

CAT#: MR229957

Cast (myc-DDK-tagged) - Mouse calpastatin (Cast), transcript variant 8

Specifications

Product Data

• Type: Mouse Tagged ORF Clone
• Tag: Myc-DDK
• Symbol: Cast
• Vector: pCMV6-Entry
• E. coli Selection: Kanamycin (25 ug/mL)
• Mammalian Cell Selection: Neomycin
• Sequence Data:
  ORF Nucleotide Sequence

  >MR229957 representing NM_001301181
  Red=Cloning site Blue=ORF Green=Tags(s)
  
  TTTTGTAATACGACTCACTATAGGGCGGCCGGGAATTCGTCGACTGGATCCGGTACCGAGGAGATCTGCC
  GCCGCGATCGCC
  
  ATGGGCCAGTTTCTGTCTTCTACTTTCTGGGAGGGCTCACCTGCTGCAGTGTGGCAAGAAAAGCTTCGTG
  AGGGTGAACGAAAGGGAGCTGGAGAAACCATCCCTATCCTCCAGGACCATGTAATATGCTCAGAAGAAAG
  GGAACATGGGTCCAAACACCATGAGGCAAAAGCAAAAGAAGAAAGGCAGGAGAAGTGTGGTGAAGATGAG
  GACACAGTCCCAGCTGAGTACAGGTTAAAACCAGCAAAGGATAAAGATGGAAAACCACTATTGCCAGAGC
  CTGAAGAAACATCTAAGAGCCTGAGTGAGTCGGAGCTGATTGGGGAGCTTTCAGCAGATTTTGACCGATC
  TACATATCAAGACAAACCATCTACGCCAGCTGAAAAGAAATCCAATGACACATCCCAAACTCCTCCGGGG
  GAGACTGTGCCTCGGGCCTCCATGTGCAGTATACGGTCAGCGCCACCCAAACTAGCATCCTTGAAGGGCG
  TGGTACCAGAAGATGCTGTTGAAACCTTGGCTGGAAGCCTGGGGACAAGGGAAGCAGATCCAGAACATGA
  AAAAACTGTGGAGGATAAAGTCAAGGAGAAAGCTAAAGAAGAAGAGCATGAAAAACTTGGTGAAAAAGAA
  GAAACAGTGCCTCCTGATTATCGACTAGAAGAAGTCAAGGATAAGGATGGAAAACCACTCCTGCCCAAAG
  AATCCCAGGAACAACTTGCACCCTTAAGCGATGACTTCCTTCTTGATGCCTTGTCTCAGGACTTCTCCAG
  TCCGGCAAACATATCGTCTCTTGAATTTGAAGATGCCAAACTTTCTGCTGCCATTTCTGAAGTAGTTTCT
  CAGACACCTGCTCCAAGCACCCATGCAGCAGCTCCACTGCCTGGCACTGAGCAGAAAGACAAAGAACTTG
  ATGATGCCTTGGATGAACTTTCTGACAGTCTTGGACAAAGGCCGCCTGATCCAGATGAGAACAAACCACT
  GGATGACAAAGTGAAGGAGAAAATCAAACCAGAGCATAGCGAGAAACTGGGAGAAAGAGACGACACCATC
  CCCCCTGAATACAGGCATCTCTTGGATAATGATGGGAAGGACAAACCAGAGAAGCCACCGACTAAGAAAA
  CAGAGAAACCTGATCAGGACCGGGACCCCATTGATGCCCTCTCAGAAGATTTGGATAGCTGCCCCTCAAC
  TACAGAGACCTCAAAGAATACAGCAAAGGGGAAGAGCAAGAAGACTTCAAGTTCCAAAGCATCCAAGGAC
  GGAGAGAAAACAAAGGACTCTTCCAAGAAGACAGAGGAAGTGTCCAAGCCAAAGGCTAAAGAAGATGCAA
  GACACAGT
  
  ACGCGTACGCGGCCGCTCGAGCAGAAACTCATCTCAGAAGAGGATCTGGCAGCAAATGATATCCTGGATT
  ACAAGGATGACGACGATAAGGTTTAA
  Protein Sequence

  >MR229957 representing NM_001301181
  Red=Cloning site Green=Tags(s)
  
  MGQFLSSTFWEGSPAAVWQEKLREGERKGAGETIPILQDHVICSEEREHGSKHHEAKAKEERQEKCGEDE
  DTVPAEYRLKPAKDKDGKPLLPEPEETSKSLSESELIGELSADFDRSTYQDKPSTPAEKKSNDTSQTPPG
  ETVPRASMCSIRSAPPKLASLKGVVPEDAVETLAGSLGTREADPEHEKTVEDKVKEKAKEEEHEKLGEKE
  ETVPPDYRLEEVKDKDGKPLLPKESQEQLAPLSDDFLLDALSQDFSSPANISSLEFEDAKLSAAISEVVS
  QTPAPSTHAAAPLPGTEQKDKELDDALDELSDSLGQRPPDPDENKPLDDKVKEKIKPEHSEKLGERDDTI
  PPEYRHLLDNDGKDKPEKPPTKKTEKPDQDRDPIDALSEDLDSCPSTTETSKNTAKGKSKKTSSSKASKD
  GEKTKDSSKKTEEVSKPKAKEDARHS
  
  TRTRPLEQKLISEEDLAANDILDYKDDDDKV
• Restriction Sites: SgfI-MluI Cloning Scheme for this gene Plasmid Map
• ACCN: NM_001301181
• ORF Size: 1338 bp
• OTI Disclaimer: The molecular sequence of this clone aligns with the gene accession number as a point of reference only. However, individual transcript sequences of the same gene can differ through naturally occurring variations (e.g. polymorphisms), each with its own valid existence. This clone is substantially in agreement with the reference, but a complete review of all prevailing variants is recommended prior to use. More info
• OTI Annotation: This clone was engineered to express the complete ORF with an expression tag. Expression varies depending on the nature of the gene.

Reference Data

• RefSeq: NM_001301181.1, NP_001288110.1
• RefSeq Size: 2973
• RefSeq ORF: 1341
• Locus ID: 12380
• MW: 49.6 kDa
• Gene Summary: This gene encodes an inhibitor of the calcium-dependent cysteine protease, calpain. This protein plays roles in multiple processes, including apoptosis, cell cycle regulation, and membrane fusion. Multiple protein isoforms exist which contain unique N-terminal domains, and multiple inhibitory domains that share homology with each other. Some isoforms may be tissue-specific. Two different pseudogenes of this gene are found on chromosome 19. [provided by RefSeq, Jul 2014]