Chka (NM_001271496) Mouse Tagged ORF Clone

CAT#: MR229984

Chka (myc-DDK-tagged) - Mouse choline kinase alpha (Chka), transcript variant 3

Specifications

Product Data

• Type: Mouse Tagged ORF Clone
• Tag: Myc-DDK
• Symbol: Chka
• Synonyms: Chetk-alpha; Chk; ChoK; CK; CK/EK-alpha; EK; EtnK-alpha
• Vector: pCMV6-Entry
• E. coli Selection: Kanamycin (25 ug/mL)
• Mammalian Cell Selection: Neomycin
• Sequence Data:
  ORF Nucleotide Sequence

  >MR229984 representing NM_001271496
  Red=Cloning site Blue=ORF Green=Tags(s)
  
  TTTTGTAATACGACTCACTATAGGGCGGCCGGGAATTCGTCGACTGGATCCGGTACCGAGGAGATCTGCC
  GCCGCGATCGCC
  
  ATGAAAACCAAGTTCTGCACCGGGGGCGAGGCCGAGCCGTCGCCTCTCGGGCTGCTGCTGAGCTGCGGTG
  GCAACGCTGCCCCGACGCCCGGCGTAGGGCAGCAGCGGGACGCCGCAGGCGAGCTGGAGTCCAAGCAGCT
  TGGTGGCCGAACCCAACCTCTCGCGCTGCCGCCGCCGCCGCCGCCGCCCCTGCCGCTGCCCCCGCCGCCA
  TCACCGCCGCTAGCGGACGAACAACCCGAGCCCCGGACCAGGCGCAGGGCCTACCTGTGGTGTAAGGAAT
  TCCTGCCCGGAGCCTGGCGAGGCCTTCGCGAGGACCAGTTCCACATCAGTGTCATCAGGGGTGGTCTCAG
  TAACATGCTGTTCCAGTGCTCCCTGCCAGACTCCATAGCCAGCGTTGGCGATGAGCCTCGGAAAGTGCTC
  TTGCGGCTCTATGGGGCAATCTTAAAGATGAGGTCCTGTAATAAAGAGGGATCCGAACAAGCTCAGAATG
  AAAATGAATTTCAAGGGGCTGAGGCGATGGTTCTGGAGAGTGTTATGTTTGCCATTCTTGCAGAGAGGTC
  ACTTGGGCCAAAACTCTTTGGCATCTTTCCCCAAGGCCGACTGGAGCAGTTTATCCCGAGCCGGCGATTG
  GACACTGAAGAGTTACGTTTACCAGATATTTCTGCAGAAATAGCTGAGAAAATGGCCACATTTCATGGTA
  TGAAAATGCCATTCAATAAGGAACCAAAATGGCTTTTTGGAACAATGGAAAAATACCTGAATCAAGTACT
  AAGACTCAAATTCAGCAGGGAGGCCAGAGTTCAGCAACTGCACAAGATCCTCTCTTACAACCTGCCTCTT
  GAGCTCGAGAACCTGAGGTCATTGCTGCAGTATACTAGATCTCCAGTTGTATTTTGTCATAATGACTGTC
  AAGAAGGTAATATCTTATTGTTGGAAGGCCAGGAGAACTCTGAAAGGCGGAAGCTGATGCTTATTGACTT
  TGAGTACAGCAGTTACAATTACAGGGGATTTGACATTGGAAATCATTTCTGTGAATGGATGTATGATTAT
  ACCTATGAGAAGTATCCTTTCTTCAGAGCGAACATTCAGAAGTATCCTTCCCGAAAACAACAGCTCCATT
  TTATTTCCAGTTACTTGACTACATTCCAAAATGATTTTGAAAGCCTCAGTAGTGAAGAGCAGTTTGCTAC
  AAAAGAAGACATGTTGCTTGAAGTCAACAGATTTGCTCTTGCCTCTCATTTCCTCTGGGGACTTTGGTCC
  ATTGTACAGGCCAAGATCTCATCCATTGAATTTGGGTACATGGAATATGCCCAAGCCAGATTCGAGGCCT
  ACTTTGACCAGAAGAGGAAGCTTGGGGTG
  
  ACGCGTACGCGGCCGCTCGAGCAGAAACTCATCTCAGAAGAGGATCTGGCAGCAAATGATATCCTGGATT
  ACAAGGATGACGACGATAAGGTTTAA
  Protein Sequence

  >MR229984 representing NM_001271496
  Red=Cloning site Green=Tags(s)
  
  MKTKFCTGGEAEPSPLGLLLSCGGNAAPTPGVGQQRDAAGELESKQLGGRTQPLALPPPPPPPLPLPPPP
  SPPLADEQPEPRTRRRAYLWCKEFLPGAWRGLREDQFHISVIRGGLSNMLFQCSLPDSIASVGDEPRKVL
  LRLYGAILKMRSCNKEGSEQAQNENEFQGAEAMVLESVMFAILAERSLGPKLFGIFPQGRLEQFIPSRRL
  DTEELRLPDISAEIAEKMATFHGMKMPFNKEPKWLFGTMEKYLNQVLRLKFSREARVQQLHKILSYNLPL
  ELENLRSLLQYTRSPVVFCHNDCQEGNILLLEGQENSERRKLMLIDFEYSSYNYRGFDIGNHFCEWMYDY
  TYEKYPFFRANIQKYPSRKQQLHFISSYLTTFQNDFESLSSEEQFATKEDMLLEVNRFALASHFLWGLWS
  IVQAKISSIEFGYMEYAQARFEAYFDQKRKLGV
  
  TRTRPLEQKLISEEDLAANDILDYKDDDDKV
• Restriction Sites: SgfI-MluI Cloning Scheme for this gene Plasmid Map
• ACCN: NM_001271496
• ORF Size: 1359 bp
• OTI Disclaimer: The molecular sequence of this clone aligns with the gene accession number as a point of reference only. However, individual transcript sequences of the same gene can differ through naturally occurring variations (e.g. polymorphisms), each with its own valid existence. This clone is substantially in agreement with the reference, but a complete review of all prevailing variants is recommended prior to use. More info
• OTI Annotation: This clone was engineered to express the complete ORF with an expression tag. Expression varies depending on the nature of the gene.

Reference Data

• RefSeq: NM_001271496.1, NP_001258425.1
• RefSeq Size: 2627
• RefSeq ORF: 1362
• Locus ID: 12660
• MW: 52.4 kDa