Ism1 (NM_001276489) Mouse Tagged ORF Clone

CAT#: MR230032

Ism1 (myc-DDK-tagged) - Mouse isthmin 1 homolog (zebrafish) (Ism1)

Specifications

Product Data

• Type: Mouse Tagged ORF Clone
• Tag: Myc-DDK
• Symbol: Ism1
• Synonyms: 5430433G21Rik; Ism
• Vector: pCMV6-Entry
• E. coli Selection: Kanamycin (25 ug/mL)
• Mammalian Cell Selection: Neomycin
• Sequence Data:
  ORF Nucleotide Sequence

  >MR230032 representing NM_001276489
  Red=Cloning site Blue=ORF Green=Tags(s)
  
  TTTTGTAATACGACTCACTATAGGGCGGCCGGGAATTCGTCGACTGGATCCGGTACCGAGGAGATCTGCC
  GCCGCGATCGCC
  
  ATGGTGCGCCTGGCTGCGGAACTGCTCCTGCTGCTGGGGCTACTGCTGCTCACGCTGCACATCACGGTTC
  TGCGCGGATCCGGAGCCTCCGACCGGCAGGACGCGGCCGCCGGCAACGTCAGCGGGTCCCAGTTGCAGAA
  TAACCTCAACCTGGAGAGTGACTCCACGTCAGAAACCAGCTTTCCTCTGTCCAAAGAAGCCCCGGAGGAG
  CACCAGGTTGTCCACCAACCCTTCCCCAGACAGCGATTCCCTCCAGAGACTGGACATCCTTCACTGCAGA
  GAGACGGCCCCAGATCCTTTCTCCTTGACCTACCAAACTTTCCAGATCTTTCCAAAGCTGATATCAATGG
  GCAGAATCCAAACATTCAGGTCACCATAGAGGTGGTTGATGGCCCTGACTCCGAAGCAGAAAAAGATCAG
  CATCCAGAGAATAAACCCAGCTGGTCGCTCCCAGCTCCCGACTGGCGGGCCTGGTGGCAGAGGTCCTTGT
  CCTTGGCCAGGACAAATAGTGGGGACCAGGATGACAAGTATGACAGTACCTCAGATGACAGCAACTTCCT
  CAGTGTTCCTAGAGGATGGGACCGTCCAGCCCCAGGACACCGGACTTTTGAAACCAAAGAGCAGCCAGAG
  TATGATTCCACAGACGGGGAGGGTGATTGGAGTCTCTGGTCTGTCTGCAGCGTCACCTGTGGAAATGGCA
  ACCAGAAACGGACCCGTTCTTGTGGCTATGCATGCATTGCCACAGAATCCAGGACCTGTGACCGTCCAAA
  CTGCCCAGGAATTGAAGATACTTTCAGGACAGCGGCCACTGAAGTGAGTCTGCTTGCGGGAAGTGAGGAG
  TTTAATGCCACCAAGTTGTTTGAAGTTGACATGGACAGCTGTGAGCGATGGATGAGCTGCAAAAGTGAGT
  TCTTAAAGAAATACATGCACAAGGTGATCAACGACCTGCCCAGCTGCCCCTGCTCCTACCCTACTGAGGT
  GGCCTACAGCACAGCTGACATCTTTGACCGCATCAAGCGCAAGGACTTCCGATGGAAGGATGCTAGTGGG
  CCCAAAGAGAAACTAGAGATCTACAAGCCTACTGCTCGGTACTGCATCCGCTCTATGCTGTCCCTGGAGA
  GTACCACACTGGCTGCCCAGCACTGTTGCTATGGTGACAACATGCAGCTCATCACCAGGGGCAAAGGGGC
  AGGCACTCCCAATCTCATCAGCACCGAGTTCTCTGCTGAACTCCACTACAAAGTGGATGTTCTGCCCTGG
  ATTATCTGCAAGGGTGACTGGAGCAGATATAATGAGGCCCGGCCTCCCAATAACGGACAGAAGTGCACAG
  AGAGCCCTTCTGATGAGGACTACATCAAACAGTTCCAAGAAGCCAGAGAGTAC
  
  ACGCGTACGCGGCCGCTCGAGCAGAAACTCATCTCAGAAGAGGATCTGGCAGCAAATGATATCCTGGATT
  ACAAGGATGACGACGATAAGGTTTAA
  Protein Sequence

  >MR230032 representing NM_001276489
  Red=Cloning site Green=Tags(s)
  
  MVRLAAELLLLLGLLLLTLHITVLRGSGASDRQDAAAGNVSGSQLQNNLNLESDSTSETSFPLSKEAPEE
  HQVVHQPFPRQRFPPETGHPSLQRDGPRSFLLDLPNFPDLSKADINGQNPNIQVTIEVVDGPDSEAEKDQ
  HPENKPSWSLPAPDWRAWWQRSLSLARTNSGDQDDKYDSTSDDSNFLSVPRGWDRPAPGHRTFETKEQPE
  YDSTDGEGDWSLWSVCSVTCGNGNQKRTRSCGYACIATESRTCDRPNCPGIEDTFRTAATEVSLLAGSEE
  FNATKLFEVDMDSCERWMSCKSEFLKKYMHKVINDLPSCPCSYPTEVAYSTADIFDRIKRKDFRWKDASG
  PKEKLEIYKPTARYCIRSMLSLESTTLAAQHCCYGDNMQLITRGKGAGTPNLISTEFSAELHYKVDVLPW
  IICKGDWSRYNEARPPNNGQKCTESPSDEDYIKQFQEAREY
  
  TRTRPLEQKLISEEDLAANDILDYKDDDDKV
• Restriction Sites: SgfI-MluI Cloning Scheme for this gene Plasmid Map
• ACCN: NM_001276489
• ORF Size: 1383 bp
• OTI Disclaimer: Due to the inherent nature of this plasmid, standard methods to replicate additional amounts of DNA in E. coli are highly likely to result in mutations and/or rearrangements. Therefore, OriGene does not guarantee the capability to replicate this plasmid DNA. Additional amounts of DNA can be purchased from OriGene with batch-specific, full-sequence verification at a reduced cost. Please contact our customer care team at custsupport@origene.com or by calling 301.340.3188 option 3 for pricing and delivery.
  
  The molecular sequence of this clone aligns with the gene accession number as a point of reference only. However, individual transcript sequences of the same gene can differ through naturally occurring variations (e.g. polymorphisms), each with its own valid existence. This clone is substantially in agreement with the reference, but a complete review of all prevailing variants is recommended prior to use. More info
• OTI Annotation: This clone was engineered to express the complete ORF with an expression tag. Expression varies depending on the nature of the gene.

Reference Data

• RefSeq: NM_001276489.1, NP_001263418.1
• RefSeq Size: 2965
• RefSeq ORF: 1386
• Locus ID: 319909
• MW: 52.3 kDa