Dclre1c (NM_001302674) Mouse Tagged ORF Clone

CAT#: MR230085

Dclre1c (myc-DDK-tagged) - Mouse DNA cross-link repair 1C, PSO2 homolog (S. cerevisiae) (Dclre1c), transcript variant 4

Specifications

Product Data

• Type: Mouse Tagged ORF Clone
• Tag: Myc-DDK
• Symbol: Dclre1c
• Synonyms: 9930121L06Rik; AI661365; Art; Snm1l
• Vector: pCMV6-Entry
• E. coli Selection: Kanamycin (25 ug/mL)
• Mammalian Cell Selection: Neomycin
• Sequence Data:
  ORF Nucleotide Sequence

  >MR230085 representing NM_001302674
  Red=Cloning site Blue=ORF Green=Tags(s)
  
  TTTTGTAATACGACTCACTATAGGGCGGCCGGGAATTCGTCGACTGGATCCGGTACCGAGGAGATCTGCC
  GCCGCGATCGCC
  
  ATGAGGCTTCGGGTGAGGAGACTTCAGACTGGCAAAAGGAGAAGCTTCCAGAATGGAGCTTCTGCACTCT
  GGAGAGTAAAAGACATCCAAAGTGTGTATTTAGACACGACTTTCTGTGACCCAAGGTTTTATCAGATCCC
  AAGTCGTGAGCAGTGTTTGAGGGGCATTTTGGAGCTGGTTCGGAGCTGGGTCACTAGGAGTCCGCACCAC
  GTCGTGTGGCTGAACTGTAAAGCAGCTTATGGCTACGAGTATTTATTCACCAACCTAAGCGAGGAGCTGG
  GAGTTCAGGTTCATGTGGACAAGCTGGACATGTTTAAAAACATGCCTGATATCCTGCACCATCTCACAAC
  GGACAGAAACACCCAGATCCACGCCTGCCGCCACCCAAAGGCAGAAGAGTGTTTTCAGTGGAATAAACTA
  CCCTGTGGTATAACTTCCCAAAACAAAACTGCACTCCACACAATCAGCATCAAGCCATCTACCATGTGGT
  TTGGAGAGAGGACCAGAAAAACCAACGTGATCGTTAGGACTGGAGAGAGCTCATACAGAGCTTGCTTCTC
  TTTTCACTCCTCCTTCAGTGAGATTAAAGATTTTTTGAGCTACATCTGCCCAGTGAATGTGTATCCAAAT
  GTCATCCCAGTTGGCCTCACTGTGGATAAGGTCATGGACGTTTTAAAGCCTCTGTGCCGGTCTCCCCAAA
  GTGTTGAGCCAAAGTACAAACCGCTTGGAAAATTGAAGAGAGCCAGAACAATCCATCTTGACTCGGAGGA
  AGACGATGATCTCTTTGATGACCCTCTACCGACACCTTTAAGGCACAAAGTTCCGTACCAGCTAACTCTT
  CAGCCTGAGCTGTTTTCAATGAAGGCACTGCCACTAGACCAGCCTGAACTGAGACAAAGCCCAGGAGGCT
  GCAAAGCAGAGAGTGTGTGGAGCCCTTCTTTGGCTAACTTCATAGACTGTGAAGAATCCAACAGCGACAG
  TGGAGAAGAGCTAGAAACCCCACCACCCTCACTGCAGGGAGGTCTGGGCCCCTCGACACTCGTCCAGCAA
  AATGCTGATCCGGATGTGGACATACCACAGTGGGAAGTCTTCTTCAAAAGAAGAGATGAAATCACAGTGG
  ACACCATGATTAGAACTCCCAGACCCAGAAAGATGAAAGGCTGTGGTCAATGGAGTCTGAAGATGCTTCT
  CCAGAACCTGGAAATACAGGAAGAGAAACATATATTTGAGAACAGGGGCTGGAAGATGGCTGGGCAGGTA
  AAGGGTAGCTGTGGTCTTTTGGAGGGCCAGAGTTCACTCCCAACATTCAAGCTAGCCACCTCCCTAGCCT
  CAAACTCCAGCTTCTGGCCTCCATGGCACCTGCATTCACATGCGCACACACAAATCTTTACCTTTAAAAA
  AAAAACCAAAACTTTACTT
  
  ACGCGTACGCGGCCGCTCGAGCAGAAACTCATCTCAGAAGAGGATCTGGCAGCAAATGATATCCTGGATT
  ACAAGGATGACGACGATAAGGTTTAA
  Protein Sequence

  >MR230085 representing NM_001302674
  Red=Cloning site Green=Tags(s)
  
  MRLRVRRLQTGKRRSFQNGASALWRVKDIQSVYLDTTFCDPRFYQIPSREQCLRGILELVRSWVTRSPHH
  VVWLNCKAAYGYEYLFTNLSEELGVQVHVDKLDMFKNMPDILHHLTTDRNTQIHACRHPKAEECFQWNKL
  PCGITSQNKTALHTISIKPSTMWFGERTRKTNVIVRTGESSYRACFSFHSSFSEIKDFLSYICPVNVYPN
  VIPVGLTVDKVMDVLKPLCRSPQSVEPKYKPLGKLKRARTIHLDSEEDDDLFDDPLPTPLRHKVPYQLTL
  QPELFSMKALPLDQPELRQSPGGCKAESVWSPSLANFIDCEESNSDSGEELETPPPSLQGGLGPSTLVQQ
  NADPDVDIPQWEVFFKRRDEITVDTMIRTPRPRKMKGCGQWSLKMLLQNLEIQEEKHIFENRGWKMAGQV
  KGSCGLLEGQSSLPTFKLATSLASNSSFWPPWHLHSHAHTQIFTFKKKTKTLL
  
  TRTRPLEQKLISEEDLAANDILDYKDDDDKV
• Restriction Sites: SgfI-MluI Cloning Scheme for this gene Plasmid Map
• ACCN: NM_001302674
• ORF Size: 1419 bp
• OTI Disclaimer: The molecular sequence of this clone aligns with the gene accession number as a point of reference only. However, individual transcript sequences of the same gene can differ through naturally occurring variations (e.g. polymorphisms), each with its own valid existence. This clone is substantially in agreement with the reference, but a complete review of all prevailing variants is recommended prior to use. More info
• OTI Annotation: This clone was engineered to express the complete ORF with an expression tag. Expression varies depending on the nature of the gene.

Reference Data

• RefSeq: NM_001302674.1, NP_001289603.1
• RefSeq Size: 3896
• RefSeq ORF: 1422
• Locus ID: 227525
• MW: 54.7 kDa
• Gene Summary: This gene encodes a member of the SNM1 family of nucleases and is involved in V(D)J recombination and DNA repair. This protein has single-strand-specific 5'-3' exonuclease activity; it also exhibits endonuclease activity on 5' and 3' overhangs and hairpins. The protein also functions in the regulation of the cell cycle in response to DNA damage. Homozygous knockout mice for this gene exhibit severe combined immunodeficiency with sensitivity to ionizing radiation. Mutations in this gene in humans can cause Athabascan-type severe combined immunodeficiency (SCIDA) and Omenn syndrome. Alternative splicing results in multiple transcript variants encoding different isoforms. [provided by RefSeq, Nov 2014]