Treh (NM_001277851) Mouse Tagged ORF Clone

CAT#: MR230317

Treh (myc-DDK-tagged) - Mouse trehalase (brush-border membrane glycoprotein) (Treh), transcript variant 3

Specifications

Product Data

• Type: Mouse Tagged ORF Clone
• Tag: Myc-DDK
• Symbol: Treh
• Synonyms: 2210412M19Rik
• Vector: pCMV6-Entry
• E. coli Selection: Kanamycin (25 ug/mL)
• Mammalian Cell Selection: Neomycin
• Sequence Data:
  ORF Nucleotide Sequence

  >MR230317 representing NM_001277851
  Red=Cloning site Blue=ORF Green=Tags(s)
  
  TTTTGTAATACGACTCACTATAGGGCGGCCGGGAATTCGTCGACTGGATCCGGTACCGAGGAGATCTGCC
  GCCGCGATCGCC
  
  ATGGAGAGCTCCTGCACCAAGTTCAGATGGCCCAGCTCTACCAAGATGACAAGCAGTTTGTGGATATGTC
  ACTGGCCACATCTCCAGATGAAGTCCTGCAGAAGTTCAGTGAGCTGGCCACAGTCCACAACCACAGCATC
  CCCAAGGAACAGCTTCAGGAATTTGTCCAGAGTCACTTCCAGCCCGTGGGGCAGGAGCTGCAGTCCTGGA
  CCCCTGAGGACTGGAAGGACAGCCCTCAGTTCCTGCAGAAGATCTCGGATGCTAATCTGCGTGTCTGGGC
  GGAGGAGCTACACAAGATCTGGAAAAAGCTGGGAAAGAAGGGACTCGTACTGGGTGATGGAAGGCCTGCT
  TCTTTCTGAGATGGCCTCAACAGTGAAGGGTATGCTGCAAAACTTTCTGGATCTGGTGAAGACCTACGGA
  CATATCCCCAACGGTGGACGCATATATTACCTGCAACGGAGCCAGCCCCCACTCCTGACTCTCATGATGG
  ATCGATATGTAGCTCATACCAAGGATGTCGCCTTCCTTCAGGAGAATATTGGGACTCTAGCCTCTGAACT
  GGACTTCTGGACTGTGAACAGGACTGTCTCTGTAGTCTCAGGAGGACAAAGCTATGTCTTAAATCGCTAC
  TATGTCCCTTATGGGGGACCCAGGCCAGAGTCCTACAGGAAAGACGCAGAATTGGCAAACTCTGTGCCAG
  AAGGGGACCGAGAGACTCTGTGGGCTGAGCTCAAGGCTGGGGCTGAGTCTGGCTGGGACTTCTCTTCACG
  CTGGCTTGTTGGAGGCCCAGACCCTGATTTGCTCAGCAGCATCCGAACCAGCAAAATGGTACCCGCTGAT
  CTGAACGCGTTCCTGTGCCAAGCAGAGGAACTGATGAGTAACTTCTACTCCAGACTAGGGAACGACACAG
  AGGCCACAAAGTACAGGAACCTGCGGGCCCAGCGCTTGGCCGCCATGGAAGCTGTCCTGTGGGACGAGCA
  GAAGGGTGCCTGGTTTGACTATGACTTGGAAAAGGGGAAGAAGAACCTGGAGTTTTATCCCTCCAACCTC
  TCCCCACTTTGGGCTGGCTGCTTCTCAGACCCTAGTGTTGCTGACAAGGCTCTGAAGTACTTGGAGGACA
  GCAAGATCTTGACCTACCAATATGGAATCCCAACCTCTCTTCGTAACACAGGCCAGCAGTGGGACTTCCC
  CAATGCCTGGGCCCCACTGCAGGACCTGGTCATTAGAGGTTTGGCCAAGTCAGCTTCCCCCCGGACTCAG
  GAGGTGGCTTTCCAGCTGGCCCAGAATTGGATCAAAACCAACTTCAAAGTCTACTCCCAAAAGTCAGCGA
  TGTTTGAGAAGTATGACATCAGCAACGGTGGACATCCAGGTGGAGGAGGGGAGTATGAAGTTCAGGAAGG
  ATTTGGCTGGACAAACGGATTGGCCCTGATGCTTCTGGATCGCTATGGTGACCAGTTGACTTCAGGGACC
  CAGTTAGCTTCCCTGGGACCCCACTGCCTAGTGGCTGCCCTTCTTCTCAGTCTTCTGCTACAG
  
  AGCGGACCGACGCGTACGCGGCCGCTCGAGCAGAAACTCATCTCAGAAGAGGATCTGGCAGCAAATGATATCC
  TGGATTACAAGGATGACGACGATAAGGTTTAA
  Protein Sequence

  >MR230317 representing NM_001277851
  Red=Cloning site Green=Tags(s)
  
  MESSCTKFRWPSSTKMTSSLWICHWPHLQMKSCRSSVSWPQSTTTASPRNSFRNLSRVTSSPWGRSCSPG
  PLRTGRTALSSCRRSRMLICVSGRRSYTRSGKSWERRDSYWVMEGLLLSEMASTVKGMLQNFLDLVKTYG
  HIPNGGRIYYLQRSQPPLLTLMMDRYVAHTKDVAFLQENIGTLASELDFWTVNRTVSVVSGGQSYVLNRY
  YVPYGGPRPESYRKDAELANSVPEGDRETLWAELKAGAESGWDFSSRWLVGGPDPDLLSSIRTSKMVPAD
  LNAFLCQAEELMSNFYSRLGNDTEATKYRNLRAQRLAAMEAVLWDEQKGAWFDYDLEKGKKNLEFYPSNL
  SPLWAGCFSDPSVADKALKYLEDSKILTYQYGIPTSLRNTGQQWDFPNAWAPLQDLVIRGLAKSASPRTQ
  EVAFQLAQNWIKTNFKVYSQKSAMFEKYDISNGGHPGGGGEYEVQEGFGWTNGLALMLLDRYGDQLTSGT
  QLASLGPHCLVAALLLSLLLQ
  
  SGPTRTRPLEQKLISEEDLAANDILDYKDDDDKV
• Restriction Sites: SgfI-RsrII Cloning Scheme for this gene Plasmid Map
• ACCN: NM_001277851
• ORF Size: 1533 bp
• OTI Disclaimer: The molecular sequence of this clone aligns with the gene accession number as a point of reference only. However, individual transcript sequences of the same gene can differ through naturally occurring variations (e.g. polymorphisms), each with its own valid existence. This clone is substantially in agreement with the reference, but a complete review of all prevailing variants is recommended prior to use. More info
• OTI Annotation: This clone was engineered to express the complete ORF with an expression tag. Expression varies depending on the nature of the gene.

Reference Data

• RefSeq: NM_001277851.1, NP_001264780.1
• RefSeq Size: 1952
• RefSeq ORF: 1536
• Locus ID: 58866
• MW: 57.9 kDa
• Gene Summary: This gene belongs to the alpha-glucosidase family, whose members encode enzymes that carry out hydrolysis of alpha-glucoside bonds of a variety of carbohydrates. The enzyme encoded by this gene uses the disaccharide trehalose as a highly specific substrate and converts it into two glucose molecules. Alternative splicing of this gene results in multiple transcript variants encoding different isoforms. [provided by RefSeq, Apr 2013]