Ppil2 (NM_001252445) Mouse Tagged ORF Clone

CAT#: MR230355

Ppil2 (myc-DDK-tagged) - Mouse peptidylprolyl isomerase (cyclophilin)-like 2 (Ppil2), transcript variant 3

Specifications

Product Data

• Type: Mouse Tagged ORF Clone
• Tag: Myc-DDK
• Symbol: Ppil2
• Synonyms: 0610009L05Rik; 1700016N17Rik; 4921520K19Rik; 4930511F14Rik; AA589416; C130078A06Rik; CYC4
• Vector: pCMV6-Entry
• E. coli Selection: Kanamycin (25 ug/mL)
• Mammalian Cell Selection: Neomycin
• Sequence Data:
  ORF Nucleotide Sequence

  >MR230355 ORF sequence
  Red=Cloning site Blue=ORF Green=Tags(s)
  
  TTTTGTAATACGACTCACTATAGGGCGGCCGGGAATTCGTCGACTGGATCCGGTACCGAGGAGATCTGCC
  GCCGCGATCGCC
  
  ATGGGGAAGCGACAGCACCAGAAGGACAAGATGTATATCACTTGTGCTGAGTACACTCATTTCTATGGTG
  GCAGGAAGCCAGATATCTCACAGACAAGTTTTCGCCGCTTACCTTTTGACCACTGCAGTCTCTCTCTCCA
  GCCTTTTGTCTACCCAGTCTGCACCCCAGAAGGTGTCGTCTTTGACTTGCTGAACATTGTTCCCTGGCTT
  AAGAAGTATGGGACGAATCCCAGCACTGGAGAGAAACTTGATGGGAAGTCCTTGATCAAGCTGAACTTCG
  CAAAGAACAGCGAAGGGCAGTACCACTGTCCAGTGCTGTATTCCGTGTTCACTGACAACACCCATATTGT
  GGCCATCAGGACAACTGGCAATGTCTACACCTATGAGGCAGTGGAGCAGCTAAACATCAAGGCCAAGAAC
  TTGCGGGATCTGTTGACTGATGAGCCCTTTTCCAGGCAAGACATCATCACCCTGCAGGACCCCACCAATT
  TGGACAAATTCAATGTTAGCAACTTCTTCCATGTGAAGAATAACATGAGAATAATAGACCCAGATGAGGA
  AAAGGCCAAACAAGACCCATCTTATTATTTGAAAAACACAAATTCGGAGACGAGAGAGACGCTACAGGAG
  CTCTACAAAGAGTTCAAAGGAGATGAGATTTTAGCAGCTACCATGAGGCCACCTGAGAAGAAGAAGGTGG
  ACCAACTGAATGCGGCCCACTACTCCACAGGGAAGGTCAGTGCATCCTTCACCTCTACTGCCATGGTGCC
  CGAGACCACGCATGAAGCAGCTGTCATTGATGAAGATGTACTGCGCTACCAGTTTGTGAAGAAAAAGGGC
  TATGTAAGGCTTCACACCAACAAGGGCGACCTTAACTTAGAGCTGCACTGTGACCTGACACCAAAAACCT
  GTGAAAACTTCATCAAGCTCTGCAAGAAACAGTATTATGATGGTACCATCTTTCACAGGTCCATCAGGAA
  CTTTGTGATCCAGGGCGGTGACCCCACAGGTACAGGCACAGGTGGAGAGTCATTCTGGGGCAAGCCTTTC
  AAAGATGAGTTCCGTCCCAACCTTTCACACACGGGCCGTGGGGTGCTCAGCATGGCCAATTCGGGGCCCA
  ACACCAACAAATCTCAGTTCTTCATCACATTCCGATCCTGCGCTTACCTGGATAAGAAGCATACCATCTT
  TGGACGGGTTGTTGGGGGCTTTGACACGCTGACAGCCATGGAGAATGTGGAGAGTGACCCTAAAACTGAC
  CGTCCTAAGGAGGAAGTGCTCATATGTACAACCACAGTGTTTGTGGACCCCTATGAGGAGGCTGATGCCC
  AGATTGCCCAGGAACGGAAGAAGACACAGCATCAGGTGGATCCAGAGGCCAAGGTCAAGATGAGTCAGCC
  CCAGCCTGGAAACCAGGGACCCCAGACATACCGCCAGGGGGTGGGCAAGTACATCCACCCTGCGGCCACG
  AAACGATCAGCAGAGGAAGAACCATCGACCAGCACTGCCACCCCCACGGCCAAGAAAAGGCCCAGTCGGG
  GCTTTGGGGACTTCAGCTCCTGG
  
  ACGCGTACGCGGCCGCTCGAGCAGAAACTCATCTCAGAAGAGGATCTGGCAGCAAATGATATCCTGGATT
  ACAAGGATGACGACGATAAGGTTTAA
  Protein Sequence

  >MR230355 protein sequence
  Red=Cloning site Green=Tags(s)
  
  MGKRQHQKDKMYITCAEYTHFYGGRKPDISQTSFRRLPFDHCSLSLQPFVYPVCTPEGVVFDLLNIVPWL
  KKYGTNPSTGEKLDGKSLIKLNFAKNSEGQYHCPVLYSVFTDNTHIVAIRTTGNVYTYEAVEQLNIKAKN
  LRDLLTDEPFSRQDIITLQDPTNLDKFNVSNFFHVKNNMRIIDPDEEKAKQDPSYYLKNTNSETRETLQE
  LYKEFKGDEILAATMRPPEKKKVDQLNAAHYSTGKVSASFTSTAMVPETTHEAAVIDEDVLRYQFVKKKG
  YVRLHTNKGDLNLELHCDLTPKTCENFIKLCKKQYYDGTIFHRSIRNFVIQGGDPTGTGTGGESFWGKPF
  KDEFRPNLSHTGRGVLSMANSGPNTNKSQFFITFRSCAYLDKKHTIFGRVVGGFDTLTAMENVESDPKTD
  RPKEEVLICTTTVFVDPYEEADAQIAQERKKTQHQVDPEAKVKMSQPQPGNQGPQTYRQGVGKYIHPAAT
  KRSAEEEPSTSTATPTAKKRPSRGFGDFSSW
  
  TRTRPLEQKLISEEDLAANDILDYKDDDDKV
• Restriction Sites: SgfI-MluI Cloning Scheme for this gene Plasmid Map
• ACCN: NM_001252445
• ORF Size: 1563 bp
• OTI Disclaimer: The molecular sequence of this clone aligns with the gene accession number as a point of reference only. However, individual transcript sequences of the same gene can differ through naturally occurring variations (e.g. polymorphisms), each with its own valid existence. This clone is substantially in agreement with the reference, but a complete review of all prevailing variants is recommended prior to use. More info
• OTI Annotation: This clone was engineered to express the complete ORF with an expression tag. Expression varies depending on the nature of the gene.
• Product Components: The ORF clone is ion-exchange column purified, transfection-ready dried plasmid DNA, and shipped with 2 vector sequencing primers.
• Reconstitution: 1. Centrifuge at 5,000xg for 5min.
  2. Carefully open the tube and add 100ul of sterile water to dissolve the DNA.
  3. Close the tube and incubate for 10 minutes at room temperature.
  4. Briefly vortex the tube and then do a quick spin (less than 5000xg) to concentrate the liquid at the bottom.
  5. Store the suspended plasmid at -20°C. The DNA is stable for at least one year from date of shipping when stored at -20°C.

Reference Data

• RefSeq: NM_001252445.1, NP_001239374.1
• RefSeq Size: 1812 bp
• RefSeq ORF: 1566 bp
• Locus ID: 66053
• MW: 59.1 kDa