Cyp4f14 (NM_001204334) Mouse Tagged ORF Clone

CAT#: MR230375

Cyp4f14 (myc-DDK-tagged) - Mouse cytochrome P450, family 4, subfamily f, polypeptide 14 (Cyp4f14), transcript variant 5

Specifications

Product Data

• Type: Mouse Tagged ORF Clone
• Tag: Myc-DDK
• Symbol: Cyp4f14
• Synonyms: 1300014O15Rik; cyp4f-14
• Vector: pCMV6-Entry
• E. coli Selection: Kanamycin (25 ug/mL)
• Mammalian Cell Selection: Neomycin
• Sequence Data:
  ORF Nucleotide Sequence

  >MR230375 ORF sequence
  Red=Cloning site Blue=ORF Green=Tags(s)
  
  TTTTGTAATACGACTCACTATAGGGCGGCCGGGAATTCGTCGACTGGATCCGGTACCGAGGAGATCTGCC
  GCCGCGATCGCC
  
  ATGTCACAGCTGAGCCTGTCCTGGTTGGGACTGGGGCCCGAGGTGGCCTTCCCATGGAAGACCCTGCTAC
  TGCTTGGGGCCTCCTGGATCCTGGCCCGGATTCTGATCCAGATCTATGCTGCCTATAGGAACTATCGTCA
  CCTCCATGGCTTCCCTCAGCCCCCCAAACGGAACTGGCTTATGGGTCACGTGGGCATGGTAACTCCTACA
  GAGCAGGGCTTGAAGGAATTGACTCGTTTGGTGGGTACCTACCCCCAAGGTTTTTTGATGTGGATTGGAC
  CAATGGTCCCTGTTATTACTCTATGCCATTCTGACATCGTCCGATCTATCCTCAATGCCTCAGCCGCTGT
  TGCACTCAAGGATGTGATATTCTACAGCATCCTAAAGCCTTGGCTGGGGGATGGGCTCTTGGTGAGTGCT
  GGTGACAAGTGGAGCCGCCATCGTCGCATGCTGACACCCGCTTTCCATTTCAACATCCTGAAGCCCTATG
  TGAAGATTTTCAACGATAGCACCAACATCATGCACGCCAAGTGGCAACGGTTGATTTCAGATGGCAGTGC
  CCGTCTGGACATGTTTGAGCACGTCAGCCTGATGACTCTGGATAGTCTGCAGAAATGTGTCTTCAGCTTT
  GACAGCAACTGTCAGGAGAAGTCCAGTGAATATATTGCTGCCATCCTGGAGCTCAGTGCCCTAGTGGCCA
  AAAGGCACCAGCAGCCTTTGATGTTCATGGACCTGCTGTATAATCTCACCCCTGATGGTATGCGCTTCCG
  TAAGGCCTGCAACGTGGTGCATGAATTCACTGATGCTGTCATCCGGGAGCGGCACCGCACCCTCCCAGAT
  CAGGGTTTGGATGACTTCCTTAAGTCTAAGGCCAAGTCCAAGACTTTGGATTTCATTGATGTGCTGCTGC
  TGAGCAAGGATGAAGATGGAAAGGAGCTGTCAGATGAGGACATCCGAGCAGAGGCTGACACCTTCATGTT
  TGAGGGCCATGACACGACAGCCAGTGGACTCTCCTGGATCCTCTACAACCTGGCAAGGCACCCAGAATAC
  CAGGAGCGCTGCCGGCAGGAAGTTCAGGAGCTGCTGAGGGGCCGAGAGCCTGAGGAGATTGAATGGGACG
  ACCTGGCCCAGCTACCCTTCCTGACCATGTGCATCAAGGAGAGTCTGCGACTGCATCCCCCAGTCACGGT
  CATCTCCCGATGCTGCACCCAGGACATTCTGCTCCCAGATGGCCGGACCATTCCCAAAGGTATCATCTGT
  CTCATCAGCATCTTTGGAATTCATCACAACCCATCCGTGTGGCCAGACCCTGAGGTCTATGACCCCTTCC
  GCTTTGACCCTGAAAACATCAAAGACAGCTCACCTCTGGCATTTATTCCCTTCTCGGCGGGACCCAGGAA
  CTGCATAGGACAGACTTTCGCCATGAGTGAGATGAAGGTGGCACTGGCGCTGACGCTGCTGCGCTTCCGA
  CTCCTACCGGATGACAAGGAGCCGCGCAGGCAGCCAGAGCTGATCCTGCGCGCAGAGGGCGGGCTGTGGC
  TGCGGGTGGAGCCGCTGAGCGCGGGCGCTCAT
  
  ACGCGTACGCGGCCGCTCGAGCAGAAACTCATCTCAGAAGAGGATCTGGCAGCAAATGATATCCTGGATT
  ACAAGGATGACGACGATAAGGTTTAA
  Protein Sequence

  >MR230375 protein sequence
  Red=Cloning site Green=Tags(s)
  
  MSQLSLSWLGLGPEVAFPWKTLLLLGASWILARILIQIYAAYRNYRHLHGFPQPPKRNWLMGHVGMVTPT
  EQGLKELTRLVGTYPQGFLMWIGPMVPVITLCHSDIVRSILNASAAVALKDVIFYSILKPWLGDGLLVSA
  GDKWSRHRRMLTPAFHFNILKPYVKIFNDSTNIMHAKWQRLISDGSARLDMFEHVSLMTLDSLQKCVFSF
  DSNCQEKSSEYIAAILELSALVAKRHQQPLMFMDLLYNLTPDGMRFRKACNVVHEFTDAVIRERHRTLPD
  QGLDDFLKSKAKSKTLDFIDVLLLSKDEDGKELSDEDIRAEADTFMFEGHDTTASGLSWILYNLARHPEY
  QERCRQEVQELLRGREPEEIEWDDLAQLPFLTMCIKESLRLHPPVTVISRCCTQDILLPDGRTIPKGIIC
  LISIFGIHHNPSVWPDPEVYDPFRFDPENIKDSSPLAFIPFSAGPRNCIGQTFAMSEMKVALALTLLRFR
  LLPDDKEPRRQPELILRAEGGLWLRVEPLSAGAH
  
  TRTRPLEQKLISEEDLAANDILDYKDDDDKV
• Restriction Sites: SgfI-MluI Cloning Scheme for this gene Plasmid Map
• ACCN: NM_001204334
• ORF Size: 1572 bp
• OTI Disclaimer: The molecular sequence of this clone aligns with the gene accession number as a point of reference only. However, individual transcript sequences of the same gene can differ through naturally occurring variations (e.g. polymorphisms), each with its own valid existence. This clone is substantially in agreement with the reference, but a complete review of all prevailing variants is recommended prior to use. More info
• OTI Annotation: This clone was engineered to express the complete ORF with an expression tag. Expression varies depending on the nature of the gene.
• Product Components: The ORF clone is ion-exchange column purified, transfection-ready dried plasmid DNA, and shipped with 2 vector sequencing primers.
• Reconstitution: 1. Centrifuge at 5,000xg for 5min.
  2. Carefully open the tube and add 100ul of sterile water to dissolve the DNA.
  3. Close the tube and incubate for 10 minutes at room temperature.
  4. Briefly vortex the tube and then do a quick spin (less than 5000xg) to concentrate the liquid at the bottom.
  5. Store the suspended plasmid at -20°C. The DNA is stable for at least one year from date of shipping when stored at -20°C.

Reference Data

• RefSeq: NM_001204334.1, NP_001191263.1
• RefSeq Size: 2051 bp
• RefSeq ORF: 1575 bp
• Locus ID: 64385
• MW: 59.8 kDa