Ubox5 (NM_001255994) Mouse Tagged ORF Clone

CAT#: MR230436

Ubox5 (myc-DDK-tagged) - Mouse U box domain containing 5 (Ubox5), transcript variant 3

Specifications

Product Data

• Type: Mouse Tagged ORF Clone
• Tag: Myc-DDK
• Symbol: Ubox5
• Synonyms: 1500010O06Rik; C330018L13Rik; Rnf37; Ubce7ip5; Ube7ip5; UIP5
• Vector: pCMV6-Entry
• E. coli Selection: Kanamycin (25 ug/mL)
• Mammalian Cell Selection: Neomycin
• Sequence Data:
  ORF Nucleotide Sequence

  >MR230436 ORF sequence
  Red=Cloning site Blue=ORF Green=Tags(s)
  
  TTTTGTAATACGACTCACTATAGGGCGGCCGGGAATTCGTCGACTGGATCCGGTACCGAGGAGATCTGCC
  GCCGCGATCGCC
  
  ATGGTAGTAAATCTTTGCCTCCCACAGTTCAGACCAAGAATTCACTGCAACAAGGTGTCAGCTGATGGTT
  ACGAAGTAGAAAATCTCATCTCTGAAGACCTCATAAAAAGAAGCCATGGCTTTAGGACAGAGTATTTCAT
  TAGACCTCCAATCTATGTGACAGTTTCCTTTCCCTTTAATGTGGAAATCTGTAGGGTTAATATAGATCTC
  ACAACTGGGGGATATCAGAATGTCTCTGGCCTGGAATTATATACATCTGCCTTATCTAGCAGAGTCTCTC
  AGGATGCCCAGGACTGCTGGACTACAGGCCCGGTGGAGACATCTGTCCCAGACAAGGAGGCCTTCACCTT
  GGTAGGCAAAGTCTTACTGAAAAACCAGAACCATGTGGTGTTCAGCCACAGGGGCTTCAAGGCCAGGCCA
  CCTTTTAGCCCGATGGAAGTCACTCTCCTCTCTCCTGCTGTTGTAGCCCAGGAACTCTGGAATAAAGGGG
  CTCTTTCCCTTAGCCATGTGGCCCACCTCAAGATCGGCATCACCCATGTGACGGGCAGTGGCATCTCTTG
  CATCAAGCGTTTAGAGGTGTGGGGTCAGCCAGCCAGGACCTGCTCTCAGGAGGTGATAAATAGTGTCTTG
  TTGATAGCCTCAGAAAGCCTGCCTCAGGACTTGGATCTGCATGCTCCAGCCTTGCCCATGGAGAGTGACT
  GTGACCCTGGGGGTCAGTCTGAGAGCCAGCACTCTCCCTGTACCTTACAGGACATGTCTGAGGTGGAAAG
  TGACGTTCCAGAGGAGTTCCTGGATCCTATCACCCTGGAGATCATGCCATGCCCCATGCTGCTGCCCTCA
  GGCAAGGTCATTGACCAGAGCACTCTGGAGAAGTGTAACCTCAGTGAAGCTGCTTGGGGCCGAGTGCCCA
  GTGACCCTTTCACAGGGCTAGCCTTTACTCCACAGTCCCAGCCCCTCCCTCATCCTTCCCTGAAAGCCCG
  GATCGACCGGTTCCTACTCCAGCACTCTATTTCAGGCTGCCGCCTGCTTGGGAGAGCACAGACTCCATCA
  GCAATGACCCCTTCTGTCATTACCCTGCCCTCAAGGAAAAGGAAGACAGAGCAGGCTGAACACAGCTCAC
  ACTACAGCCTTGGCATGAGTGCGTCTTCCTCTGCCACAAGCCCTTTATTCTCACCTACTACCTCAGAGCC
  AACTGCCAAGAAGATGAAAGCCACCAGTGAGCTTGGCCTGACGGACATGGACTGTTCAGCAGGTCCAGTG
  TCCCATGAGCAGAAGCTGGCGCAAAGTCTGGAAATTGCCCTGACTTCTACCCTTGGCTCCATGCCTTCCT
  TCACTGCCCGGCTCACCAAAGGGCAGCTCCAGCTGGGCACAAGAGGGAGCAGTGCTTGCAGGAGGCCAGC
  CTCCAGCTCCGAGCATCCAAGGAGTGTTTCTGGCCCAGAGTGTGCCTCCTGCAAACAAGCATTTTCTTCC
  TACTCCACAAATGAGCCTGTGTACCAGCTGCCCTGTGGTCACCTTCTGTGCCGACCCTGCCTGAGTGAGA
  AGCAACGTTCCCAGCCCATGATGTGCACAGCCTGTCGGCAGCCAGTCACCAGCCAGGATGTGCTGCGGGT
  CCATTTC
  
  ACGCGTACGCGGCCGCTCGAGCAGAAACTCATCTCAGAAGAGGATCTGGCAGCAAATGATATCCTGGATT
  ACAAGGATGACGACGATAAGGTTTAA
  Protein Sequence

  >MR230436 protein sequence
  Red=Cloning site Green=Tags(s)
  
  MVVNLCLPQFRPRIHCNKVSADGYEVENLISEDLIKRSHGFRTEYFIRPPIYVTVSFPFNVEICRVNIDL
  TTGGYQNVSGLELYTSALSSRVSQDAQDCWTTGPVETSVPDKEAFTLVGKVLLKNQNHVVFSHRGFKARP
  PFSPMEVTLLSPAVVAQELWNKGALSLSHVAHLKIGITHVTGSGISCIKRLEVWGQPARTCSQEVINSVL
  LIASESLPQDLDLHAPALPMESDCDPGGQSESQHSPCTLQDMSEVESDVPEEFLDPITLEIMPCPMLLPS
  GKVIDQSTLEKCNLSEAAWGRVPSDPFTGLAFTPQSQPLPHPSLKARIDRFLLQHSISGCRLLGRAQTPS
  AMTPSVITLPSRKRKTEQAEHSSHYSLGMSASSSATSPLFSPTTSEPTAKKMKATSELGLTDMDCSAGPV
  SHEQKLAQSLEIALTSTLGSMPSFTARLTKGQLQLGTRGSSACRRPASSSEHPRSVSGPECASCKQAFSS
  YSTNEPVYQLPCGHLLCRPCLSEKQRSQPMMCTACRQPVTSQDVLRVHF
  
  TRTRPLEQKLISEEDLAANDILDYKDDDDKV
• Restriction Sites: SgfI-MluI Cloning Scheme for this gene Plasmid Map
• ACCN: NM_001255994
• ORF Size: 1617 bp
• OTI Disclaimer: The molecular sequence of this clone aligns with the gene accession number as a point of reference only. However, individual transcript sequences of the same gene can differ through naturally occurring variations (e.g. polymorphisms), each with its own valid existence. This clone is substantially in agreement with the reference, but a complete review of all prevailing variants is recommended prior to use. More info
• OTI Annotation: This clone was engineered to express the complete ORF with an expression tag. Expression varies depending on the nature of the gene.
• Product Components: The ORF clone is ion-exchange column purified, transfection-ready dried plasmid DNA, and shipped with 2 vector sequencing primers.
• Reconstitution: 1. Centrifuge at 5,000xg for 5min.
  2. Carefully open the tube and add 100ul of sterile water to dissolve the DNA.
  3. Close the tube and incubate for 10 minutes at room temperature.
  4. Briefly vortex the tube and then do a quick spin (less than 5000xg) to concentrate the liquid at the bottom.
  5. Store the suspended plasmid at -20°C. The DNA is stable for at least one year from date of shipping when stored at -20°C.

Reference Data

• RefSeq: NM_001255994.1, NP_001242923.1
• RefSeq Size: 3753 bp
• RefSeq ORF: 1620 bp
• Locus ID: 140629
• MW: 58.7 kDa