Klc1 (NM_001025358) Mouse Tagged ORF Clone
CAT#: MR230476
- TrueORF®
Klc1 (myc-DDK-tagged) - Mouse kinesin light chain 1 (Klc1), transcript variant b
"NM_001025358" in other vectors (1)
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Product Images
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Specifications
Product Data | |
Type | Mouse Tagged ORF Clone |
Tag | Myc-DDK |
Symbol | Klc1 |
Synonyms | AI874768; Kns2 |
Vector | pCMV6-Entry |
E. coli Selection | Kanamycin (25 ug/mL) |
Mammalian Cell Selection | Neomycin |
Sequence Data |
>MR230476 representing NM_001025358
Red=Cloning site Blue=ORF Green=Tags(s) TTTTGTAATACGACTCACTATAGGGCGGCCGGGAATTCGTCGACTGGATCCGGTACCGAGGAGATCTGCC GCCGCGATCGCC ATGTATGACAACATGTCCACCATGGTGTACATAAAGGAAGAGAAGCTGGAGAAGCTCACGCAGGATGAGA TCATCTCTAAGACCAAGCAAGTGATCCAGGGGCTGGAAGCCCTGAAGAATGAGCACAACTCCATCCTGCA GAGTTTGCTGGAGACGCTGAAGTGCTTGAAGAAGGATGACGAGAGCAACCTGGTGGAAGAGAAATCCAGC ATGATCCGCAAGTCCCTGGAGATGCTGGAGCTTGGCCTGAGCGAGGCGCAGGTGATGATGGCGCTGTCCA ATCACCTGAATGCTGTGGAGTCCGAGAAGCAAAAGCTCCGCGCTCAGGTTCGACGGCTGTGCCAGGAGAA CCAGTGGCTGCGGGATGAGCTGGCCAACACGCAGCAGAAGTTGCAGAAGAGCGAGCAGTCGGTGGCGCAG CTGGAGGAGGAGAAGAAACACCTGGAGTTCATGAACCAGCTGAAGAAGTACGACGACGACATCTCCCCCT CGGAGGACAAAGACTCTGATTCTTCCAAAGAGCCGTTGGATGATCTCTTCCCAAATGACGAGGACGAACC AGGACAAGGAATCCAGCAGCAGCACAGTAGTGCTGCGGCCGCCGCCCAGCAGGGCGGCTACGAGATCCCT GCAAGGCTGCGCACGCTCCACAACTTGGTGATCCAGTATGCTTCACAGGGGCGTTACGAGGTGGCGGTGC CACTCTGCAAGCAGGCCCTGGAGGATCTGGAGAAGACTTCCGGCCACGACCACCCCGATGTGGCTACCAT GCTCAACATCTTGGCCCTGGTGTACAGGGATCAGAACAAGTATAAAGATGCAGCTAACCTCCTGAACGAC GCCCTGGCTATCCGCGAGAAAACCCTGGGCAGAGATCACCCCGCGGTGGCAGCGACTCTGAACAACCTAG CAGTACTGTACGGTAAGCGAGGGAAGTACAAGGAGGCGGAGCCGCTGTGTAAACGAGCCCTGGAGATCAG GGAGAAGGTTCTGGGAAAGGATCATCCTGATGTTGCCAAACAGTTAAATAACCTGGCCCTGCTGTGCCAG AACCAGGGCAAGTACGAGGAGGTGGAGTATTATTACCAGAGGGCCCTGGGCATCTACCAGACGAAGCTGG GGCCCGACGATCCCAACGTGGCCAAGACCAAGAACAACCTGGCCTCCTGTTATCTGAAACAAGGGAAGTT CAAGCAGGCAGAAACGCTGTACAAGGAGATTCTCACCCGCGCACACGAGCGGGAGTTTGGATCTGTGGAC GACGAGAACAAGCCCATCTGGATGCACGCTGAAGAGAGAGAGGAGTGCAAAGGCAAGCAGAAGGACGGGT CGGCTTTTGGAGAGTATGGCGGCTGGTATAAAGCCTGCAAAGTGGACAGTCCCACCGTCACAACCACCTT GAAAAACCTTGGAGCACTTTACCGACGGCAGGGGAAGTTTGAAGCTGCAGAGACATTGGAAGAAGCCGCC ATGAGGTCACGTAAGCAGGGTCTTGACAATGTTCACAAACAGAGAGTGGCTGAAGTGCTAAATGACCCTG AGAGCATGGAGAAGCGGAGGAGCCGGGAGAGTCTCAATATGGACGTGGTCAAGTACGAGAGTGGCCCTGA CGGAGGGGAGGAAATGAGAAAGATGAAGCTCGGGCTGGTTAAA ACGCGTACGCGGCCGCTCGAGCAGAAACTCATCTCAGAAGAGGATCTGGCAGCAAATGATATCCTGGATT ACAAGGATGACGACGATAAGGTTTAA >MR230476 representing NM_001025358
Red=Cloning site Green=Tags(s) MYDNMSTMVYIKEEKLEKLTQDEIISKTKQVIQGLEALKNEHNSILQSLLETLKCLKKDDESNLVEEKSS MIRKSLEMLELGLSEAQVMMALSNHLNAVESEKQKLRAQVRRLCQENQWLRDELANTQQKLQKSEQSVAQ LEEEKKHLEFMNQLKKYDDDISPSEDKDSDSSKEPLDDLFPNDEDEPGQGIQQQHSSAAAAAQQGGYEIP ARLRTLHNLVIQYASQGRYEVAVPLCKQALEDLEKTSGHDHPDVATMLNILALVYRDQNKYKDAANLLND ALAIREKTLGRDHPAVAATLNNLAVLYGKRGKYKEAEPLCKRALEIREKVLGKDHPDVAKQLNNLALLCQ NQGKYEEVEYYYQRALGIYQTKLGPDDPNVAKTKNNLASCYLKQGKFKQAETLYKEILTRAHEREFGSVD DENKPIWMHAEEREECKGKQKDGSAFGEYGGWYKACKVDSPTVTTTLKNLGALYRRQGKFEAAETLEEAA MRSRKQGLDNVHKQRVAEVLNDPESMEKRRSRESLNMDVVKYESGPDGGEEMRKMKLGLVK TRTRPLEQKLISEEDLAANDILDYKDDDDKV |
Restriction Sites |
SgfI-MluI
Cloning Scheme for this gene
Plasmid Map
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ACCN | NM_001025358 |
ORF Size | 1653 bp |
OTI Disclaimer | The molecular sequence of this clone aligns with the gene accession number as a point of reference only. However, individual transcript sequences of the same gene can differ through naturally occurring variations (e.g. polymorphisms), each with its own valid existence. This clone is substantially in agreement with the reference, but a complete review of all prevailing variants is recommended prior to use. More info |
OTI Annotation | This clone was engineered to express the complete ORF with an expression tag. Expression varies depending on the nature of the gene. |
Product Components | The ORF clone is ion-exchange column purified, transfection-ready dried plasmid DNA, and shipped with 2 vector sequencing primers. |
Reconstitution | 1. Centrifuge at 5,000xg for 5min. 2. Carefully open the tube and add 100ul of sterile water to dissolve the DNA. 3. Close the tube and incubate for 10 minutes at room temperature. 4. Briefly vortex the tube and then do a quick spin (less than 5000xg) to concentrate the liquid at the bottom. 5. Store the suspended plasmid at -20°C. The DNA is stable for at least one year from date of shipping when stored at -20°C. |
Reference Data | |
RefSeq | NM_001025358.2, NP_001020529.2 |
RefSeq Size | 2430 bp |
RefSeq ORF | 1656 bp |
Locus ID | 16593 |
Cytogenetics | 12 61.13 cM |
MW | 63.2 kDa |
Gene Summary | Conventional kinesin is a tetrameric molecule composed of two heavy chains and two light chains, and transports various cargos along microtubules toward their plus ends. The heavy chains provide the motor activity, while the light chains bind to various cargos. This gene encodes a member of the kinesin light chain family. It associates with kinesin heavy chain through an N-terminal domain, and six tetratricopeptide repeat (TPR) motifs are thought to be involved in binding of cargos such as vesicles, mitochondria, and the Golgi complex. Thus, kinesin light chains function as adapter molecules and not motors per se. Although previously named "kinesin 2", this gene is not a member of the kinesin-2 / kinesin heavy chain subfamily of kinesin motor proteins. Extensive alternative splicing produces isoforms with different C-termini that are proposed to bind to different cargos; however, the full-length nature of some of these variants has not been determined. [provided by RefSeq, Jul 2008] |
Documents
Product Manuals |
FAQs |
Resources
Other Versions
SKU | Description | Size | Price |
---|---|---|---|
MC228265 | Klc1 (untagged) - Mouse kinesin light chain 1 (Klc1), transcript variant b |
USD 570.00 |
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