Coch (NM_001198835) Mouse Tagged ORF Clone

CAT#: MR230479

Coch (myc-DDK-tagged) - Mouse coagulation factor C homolog (Limulus polyphemus) (Coch), transcript variant 2

Specifications

Product Data

• Type: Mouse Tagged ORF Clone
• Tag: Myc-DDK
• Symbol: Coch
• Synonyms: AW122937; Coch-5B2; D12H14S564E
• Vector: pCMV6-Entry
• E. coli Selection: Kanamycin (25 ug/mL)
• Mammalian Cell Selection: Neomycin
• Sequence Data:
  ORF Nucleotide Sequence

  >MR230479 ORF sequence
  Red=Cloning site Blue=ORF Green=Tags(s)
  
  TTTTGTAATACGACTCACTATAGGGCGGCCGGGAATTCGTCGACTGGATCCGGTACCGAGGAGATCTGCC
  GCCGCGATCGCC
  
  ATGCCCTCGTCCAGGATCCCTGCTCTCTGCCTCGGTGCGTGGCTGCTGCTGCTGCTGCTGCCCCGGTTCG
  CGCGCGCCGAGGGAGCGGTTCCCATTCCTGTCACCTGCTTTACCAGAGGCCTGGATATCCGAAAAGAGAA
  AGCAGATGTTCTCTGCCCAGGAGGCTGCTCTCTTGAGGAATTCTCTGTGTTTGGGAACATAGTGTATGCG
  TCAGTGTCCAGCATCTGCGGCGCTGCTGTCCATAGGGGAGTGATTGGCACCTCAGGGGGACCTGTGCGTG
  TCTACAGCCTTCCTGGTCGAGAGAACTACTCCTCGGTAGATGCCAACGGCATCCAGTCTCAGATGCTTTC
  CCGATGGTCCGCGTCCTTCGCTGTGACCAAAGGCAAAAGCAGTACCCAGGAAGCCACAGGACGGGCAGTG
  TCCACAGCCCACCCACCTTCAGGTAAAAGACTAAAGAAGACACCAGAGAAGAAGACTGGCAACAAAGACT
  GTAAGGCAGACATTGCATTTCTCATTGATGGAAGCTTCAATATTGGGCAGCGCCGATTTAATTTGCAGAA
  GAATTTTGTTGGGAAAGTGGCACTAATGTTGGGAATTGGAACAGAAGGACCACACGTGGGTCTCGTTCAA
  GCCAGTGAACACCCCAAAATAGAATTTTACTTGAAAAACTTTACTTCAGCCAAAGATGTCTTGTTTGCCA
  TAAAAGAAGTAGGTTTCCGAGGGGGTAACTCCAACACAGGAAAAGCCTTGAAGCACACTGCTCAGAAATT
  CTTTACAGCAGACACTGGTGTGAGAAAAGGAATACCAAAAGTGGTGGTAGTGTTTATTGATGGTTGGCCC
  TCTGATGACATTGAGGAAGCAGGCATTGTGGCCAGAGAGTTTGGTGTCAATGTATTTATAGTTTCTGTGG
  CCAAGCCCATTCCTGAAGAACTGGGGATGGTTCAAGATGTTGCATTTGTTGACAAGGCTGTGTGTCGGAA
  TAATGGCTTCTTCTCTTATCACATGCCCAACTGGTTTGGCACTACAAAATATGTGAAGCCTCTGGTGCAG
  AAGCTCTGTACGCACGAACAGATGATGTGCAGCAAAACCTGCTACAACTCAGTGAACATTGCCTTTCTGA
  TTGACGGCTCCAGCAGTGTTGGAGATAGCAATTTCCGCCTCATGCTAGAATTTGTTTCTAACATAGCGAA
  GACATTTGAAATCTCAGACATTGGAGCCAAGATAGCTGCTGTACAGTTCACTTATGACCAGCGCACCGAG
  TTCAGTTTCACTGACTATAATACCAAAGAGAACGTCCTAGCTGTCCTAGCGAACATCCGCTACATGAGTG
  GTGGCACAGCTACTGGTGATGCCATCGCCTTTACTGTTAGAAATGTATTTGGTCCCATAAGGGACAGCCC
  CAACAAAAACTTCCTGGTTATTGTCACAGATGGGCAGTCCTATGATGATGTCCGAGGCCCTGCTGCAGCT
  GCCCATGATGCAGGTATCACCATCTTCTCTGTTGGTGTGGCTTGGGCACCGCTGGATGACCTGAGAGATA
  TGGCCTCTAAACCCAAAGAGTCACACGCTTTCTTTACCAGAGAGTTCACAGGGTTAGAACCAATTGTCTC
  TGACGTCATCAGAGGCATTTGTAGAGACTTCTTAGAATCCCAGCAA
  
  ACGCGTACGCGGCCGCTCGAGCAGAAACTCATCTCAGAAGAGGATCTGGCAGCAAATGATATCCTGGATT
  ACAAGGATGACGACGATAAGGTTTAA
  Protein Sequence

  >MR230479 protein sequence
  Red=Cloning site Green=Tags(s)
  
  MPSSRIPALCLGAWLLLLLLPRFARAEGAVPIPVTCFTRGLDIRKEKADVLCPGGCSLEEFSVFGNIVYA
  SVSSICGAAVHRGVIGTSGGPVRVYSLPGRENYSSVDANGIQSQMLSRWSASFAVTKGKSSTQEATGRAV
  STAHPPSGKRLKKTPEKKTGNKDCKADIAFLIDGSFNIGQRRFNLQKNFVGKVALMLGIGTEGPHVGLVQ
  ASEHPKIEFYLKNFTSAKDVLFAIKEVGFRGGNSNTGKALKHTAQKFFTADTGVRKGIPKVVVVFIDGWP
  SDDIEEAGIVAREFGVNVFIVSVAKPIPEELGMVQDVAFVDKAVCRNNGFFSYHMPNWFGTTKYVKPLVQ
  KLCTHEQMMCSKTCYNSVNIAFLIDGSSSVGDSNFRLMLEFVSNIAKTFEISDIGAKIAAVQFTYDQRTE
  FSFTDYNTKENVLAVLANIRYMSGGTATGDAIAFTVRNVFGPIRDSPNKNFLVIVTDGQSYDDVRGPAAA
  AHDAGITIFSVGVAWAPLDDLRDMASKPKESHAFFTREFTGLEPIVSDVIRGICRDFLESQQ
  
  TRTRPLEQKLISEEDLAANDILDYKDDDDKV
• Restriction Sites: SgfI-MluI Cloning Scheme for this gene Plasmid Map
• ACCN: NM_001198835
• ORF Size: 1659 bp
• OTI Disclaimer: The molecular sequence of this clone aligns with the gene accession number as a point of reference only. However, individual transcript sequences of the same gene can differ through naturally occurring variations (e.g. polymorphisms), each with its own valid existence. This clone is substantially in agreement with the reference, but a complete review of all prevailing variants is recommended prior to use. More info
• OTI Annotation: This clone was engineered to express the complete ORF with an expression tag. Expression varies depending on the nature of the gene.
• Product Components: The ORF clone is ion-exchange column purified, transfection-ready dried plasmid DNA, and shipped with 2 vector sequencing primers.
• Reconstitution: 1. Centrifuge at 5,000xg for 5min.
  2. Carefully open the tube and add 100ul of sterile water to dissolve the DNA.
  3. Close the tube and incubate for 10 minutes at room temperature.
  4. Briefly vortex the tube and then do a quick spin (less than 5000xg) to concentrate the liquid at the bottom.
  5. Store the suspended plasmid at -20°C. The DNA is stable for at least one year from date of shipping when stored at -20°C.

Reference Data

• RefSeq: NM_001198835.1, NP_001185764.1
• RefSeq Size: 2484 bp
• RefSeq ORF: 1659 bp
• Locus ID: 12810
• MW: 59.9 kDa