Farsb (NM_001278075) Mouse Tagged ORF Clone

CAT#: MR230602

Farsb (myc-DDK-tagged) - Mouse phenylalanyl-tRNA synthetase, beta subunit (Farsb), transcript variant 2

Specifications

Product Data

• Type: Mouse Tagged ORF Clone
• Tag: Myc-DDK
• Symbol: Farsb
• Synonyms: C76708; Farsa; Farsl; Farslb; Frsb
• Vector: pCMV6-Entry
• E. coli Selection: Kanamycin (25 ug/mL)
• Mammalian Cell Selection: Neomycin
• Sequence Data:
  ORF Nucleotide Sequence

  >MR230602 representing NM_001278075
  Red=Cloning site Blue=ORF Green=Tags(s)
  
  TTTTGTAATACGACTCACTATAGGGCGGCCGGGAATTCGTCGACTGGATCCGGTACCGAGGAGATCTGCC
  GCCGCGATCGCC
  
  ATGCCGACTGTCAGCGTAAAGCGAGACCTGCTCTTCCAAGCCCTGGGCCGGACCTACACTGATGAAGAGT
  TTGACGAACTGTGTTTTGAATTTGGTCTGGAACTTGATGAAATTACTTCTGAGAAGCAAATCATAAGCAA
  GGAACAAGGTCATGGGAAGGCACAGGGGGCCTCAGATGTTGTGCTTTACAAGATTGACGTCCCTGCCAAC
  AGATACGACCTCCTGTGTCTGGAAGGACTGGCTCGGGGCCTCCAGGTCTTCAAAGAAAGGATCAAAGCTC
  CGGTATATAAGCGAGTGATGCCCAAGGGAGACATCCAAAAGCTGGTTATCACAGAAGAGACAGCCAAAGT
  GCGCCCTTTTGCTGTAGCAGCAGTTCTCCGGAATATCAAGTTCACTAAAGACCGATATGACAGCTTCATT
  GAACTTCAGGAGAAACTACACCAGAATATCTGCAGGAAAAGAGCGTTGGTTGCTATTGGAACTCACGACT
  TGGACACTTTATCAGGCCCATTTACTTACACCGCGAAACGTCCTTCAGATATCAAATTCAAGCCTCTGAA
  TAAGACCAAGGAGTATACAGCTTGTGAATTAATGAACATATACAAGACTGACAACCACCTTAAACATTAT
  TTACATATCATTGAAAGTAAGCCCCTGTATCCAGTTATCTATGACAGCAATGGTGTGGTCCTGTCGATGC
  CTCCGATTATAAATGGGAATCATTCAAAAATAACAGTAAATACTAGGAATATATTTATTGAATGCACAGG
  GACTGACTTCACTAAGGCAAAAATCGTGCTTGACATTATTGTCACCATGTTCAGTGAACACTGTGAAAAT
  CAGTTTACAGTTGAAGCAGTTGAAGTAGTTTCTCCTAATGGAAAATCAAGTACCTTTCCAGAGCTGCCTT
  ACAGAAAGGAGATGGTGAGAGCTGATTTAATTAACAAGAAAGTTGGAATCAGAGAAACTCCTGCAAATCT
  CGCCAAGCTCCTGACCAGGATGTGTCTGAAGTCAGAAGTCATAGGTGACGGCAATCAGATTGAGGTTGAA
  ATTCCTCCGACCAGAGCAGATGTCATCCATGCATGTGACATTGTGGAGGACGCAGCTATTGCTTATGGCT
  ATAACAACATTCAGATGACTCTGCCGAAAACGTACACCATAGCTAATCAATTTCCTCTCAACAAGCTCAC
  CGAGCTCCTCAGACTTGACATGGCTGCGGCTGGCTTCACCGAAGCACTGACTTTTGCTCTGTGCTCCCAA
  GAAGATATTGCTGACAAGCTTGGCCTGGATATCTCTGCAACAAAGGCAGTGCACATAAGTAATCCCAAAA
  CCGCTGAGTTCCAGGTGGCGCGCACTACCCTTCTTCCTGGCCTTCTAAAGACCATTGCAGCAAACCGGAA
  GATGCCCCTTCCTCTCAAACTGTTTGAAATCTCTGACGTTGTAGTAAAAGATTCTGGCAAAGATGTAGGT
  GCGAAAAACTACAGGCATCTCTGTGCTGTTTATTACAGCAAGACTCCCGGGTTTGAGATCATCCATGGCT
  TGCTGGACAGAATTATGCAGCTGCTCGATGTGCCTCCTGGTGAGGAAAGCGGGGGCTACATGATCAAAGC
  GTCTGCAGGTTCTGCATTTTTCCCTGGACGGTGTGCTGAGATCTTTGTTGGGGGTCAAAGCATTGGGAAG
  CTAGGCGTCCTTCATCCTGATGTTATCACCAAATTTGAGCTGACCATGCCCTGTTCCTCTCTGGAAATCA
  ATATTGAGCCCTTTTTG
  
  ACGCGTACGCGGCCGCTCGAGCAGAAACTCATCTCAGAAGAGGATCTGGCAGCAAATGATATCCTGGATT
  ACAAGGATGACGACGATAAGGTTTAA
  Protein Sequence

  >MR230602 representing NM_001278075
  Red=Cloning site Green=Tags(s)
  
  MPTVSVKRDLLFQALGRTYTDEEFDELCFEFGLELDEITSEKQIISKEQGHGKAQGASDVVLYKIDVPAN
  RYDLLCLEGLARGLQVFKERIKAPVYKRVMPKGDIQKLVITEETAKVRPFAVAAVLRNIKFTKDRYDSFI
  ELQEKLHQNICRKRALVAIGTHDLDTLSGPFTYTAKRPSDIKFKPLNKTKEYTACELMNIYKTDNHLKHY
  LHIIESKPLYPVIYDSNGVVLSMPPIINGNHSKITVNTRNIFIECTGTDFTKAKIVLDIIVTMFSEHCEN
  QFTVEAVEVVSPNGKSSTFPELPYRKEMVRADLINKKVGIRETPANLAKLLTRMCLKSEVIGDGNQIEVE
  IPPTRADVIHACDIVEDAAIAYGYNNIQMTLPKTYTIANQFPLNKLTELLRLDMAAAGFTEALTFALCSQ
  EDIADKLGLDISATKAVHISNPKTAEFQVARTTLLPGLLKTIAANRKMPLPLKLFEISDVVVKDSGKDVG
  AKNYRHLCAVYYSKTPGFEIIHGLLDRIMQLLDVPPGEESGGYMIKASAGSAFFPGRCAEIFVGGQSIGK
  LGVLHPDVITKFELTMPCSSLEINIEPFL
  
  TRTRPLEQKLISEEDLAANDILDYKDDDDKV
• Restriction Sites: SgfI-MluI Cloning Scheme for this gene Plasmid Map
• ACCN: NM_001278075
• ORF Size: 1767 bp
• OTI Disclaimer: The molecular sequence of this clone aligns with the gene accession number as a point of reference only. However, individual transcript sequences of the same gene can differ through naturally occurring variations (e.g. polymorphisms), each with its own valid existence. This clone is substantially in agreement with the reference, but a complete review of all prevailing variants is recommended prior to use. More info
• OTI Annotation: This clone was engineered to express the complete ORF with an expression tag. Expression varies depending on the nature of the gene.
• Product Components: The ORF clone is ion-exchange column purified, transfection-ready dried plasmid DNA, and shipped with 2 vector sequencing primers.
• Reconstitution: 1. Centrifuge at 5,000xg for 5min.
  2. Carefully open the tube and add 100ul of sterile water to dissolve the DNA.
  3. Close the tube and incubate for 10 minutes at room temperature.
  4. Briefly vortex the tube and then do a quick spin (less than 5000xg) to concentrate the liquid at the bottom.
  5. Store the suspended plasmid at -20°C. The DNA is stable for at least one year from date of shipping when stored at -20°C.

Reference Data

• RefSeq: NM_001278075.1, NP_001265004.1
• RefSeq Size: 2091 bp
• RefSeq ORF: 1770 bp
• Locus ID: 23874
• MW: 65.7 kDa