Capn5 (NM_001301250) Mouse Tagged ORF Clone

CAT#: MR230759

Capn5 (myc-DDK-tagged) - Mouse calpain 5 (Capn5), transcript variant 2

Specifications

Product Data

• Type: Mouse Tagged ORF Clone
• Tag: Myc-DDK
• Symbol: Capn5
• Synonyms: nCL-3
• Vector: pCMV6-Entry
• E. coli Selection: Kanamycin (25 ug/mL)
• Mammalian Cell Selection: Neomycin
• Sequence Data:
  ORF Nucleotide Sequence

  >MR230759 representing NM_001301250
  Red=Cloning site Blue=ORF Green=Tags(s)
  
  TTTTGTAATACGACTCACTATAGGGCGGCCGGGAATTCGTCGACTGGATCCGGTACCGAGGAGATCTGCC
  GCCGCGATCGCC
  
  ATGTTCTCCTGCGCGAAGGCCTATGAGGACCAGAACTACTCGGCGCTGAAGCGGGCCTGCCTGCGCAAGA
  AGGTGCTGTTCGAGGATCCCCTCTTCCCTGCCACCGACGACTCCCTTTACTATAAGGGCACCCCAGGGCC
  CACAGTCAGGTGGAAGCGGCCTAAGGATATCTGCGACGATCCCCGGCTCTTCGTAGATGGCATCAGCTCC
  CATGACCTGCACCAGGGCCAGGTGGGCAACTGCTGGTTTGTGGCTGCCTGCTCATCACTGGCCTCCCGAG
  AGTCACTCTGGCAGAAGGTCATCCCAGACTGGAAGGAGCAGGAATGGAACCCCGAGAAGCCTGACAGCTA
  TGCTGGCATCTTCCACTTCAACTTCTGGCGCTTTGGGGAGTGGGTGGACGTAATCGTCGATGACCGGCTG
  CCCACAGTCAACAACCAGCTCATTTACTGCCATTCCAACTCCAAAAATGAGTTCTGGTGTGCCCTGGTGG
  AGAAGGCCTATGCCAAGCTGGCCGGCTGTTACCAGGCCCTGGACGGAGGCAACACGGCCGATGCATTGGT
  GGATTTCACAGGTGGTGTTTCTGAACCCATTGACCTGACCGAGGGGGACTTGGCCACTGACGAGGCTAAG
  AGGAATCAGCTCTTTGAGCGAGTGCTGAAGGTGCACAGCAGAGGCGGGCTCATCAGTGCCTCCATCAAGG
  CTGTGACAGCAGCTGACATGGAGGCCCGCCTGGCATGTGGCCTGGTGAAGGGCCATGCATACGCTGTCAC
  CGATGTGCGCAAGGTGCGCCTGGGCCATGGCCTGCTGGCCTTCTTCAAGTCAGAGAAGCTTGATATGATC
  CGTCTGAGGAACCCCTGGGGCGAGCGGGAGTGGACGGGGCCCTGGAGTGACACGTCAGAGGAATGGCAGA
  AAGTGAGCAAGAGTGAGAGGGAGAAGATGGGCGTGACCGTGCAGGATGATGGGGAATTCTGGATGACCTT
  TGAGGACATGTGCCGGTACTTTACTGACATCATTAAATGCCGCCTGATTAACACGTCCTACCTGAGCATC
  CATAAGACATGGGAGGAGGCCCGGCTGCATGGTGCCTGGACGAGACATGAGGACCCACAGCAGAACCGCA
  GTGGAGGCTGCATCAACCACAAGGACACTTTCTTCCAGAACCCACAGTACGTATTTGAAGTCAAGAAGCC
  AGAAGATGAAGTGTTGATCAGTATCCAGCAGCGGCCGAAGCGCTCAACTCGCCGGGAGGGCAAAGGCGAG
  AATCTGGCCATTGGCTTCGACATCTATAAGGTGGAAGAGAACCGCCAATACCGTATGCACAGCCTACAGC
  ATAAGGCCGCCAGCTCCATCTACATCAATTCCCGCAGCGTTTTTTTGAGGACAGAGCTGCCCGAGGGCCG
  CTACGTTATCATCCCTACCACCTTTGAGCCAGGCCACACTGGCGAGTTCCTGCTCCGAGTCTTCACAGAT
  GTCCCCTCCAACTGCCGGGAACTACGCCTGGATGAGCCCCCTCGGACCTGTTGGAGTTCCCTCTGTGGCT
  ACCCTCAGCAGGTGGCCCAGGTACATGTCCTGGGGGCTGCTGGCCTCAAGGACTCCCCAACAGGAGCAAA
  CTCATATGTGATCATCAAGTGTGAGGGCGAAAAGGTTCGCTCAGCTGTGCAGAGAGGGACCTCGACACCA
  GAGTACAATGTAAAAGGCATCTTCTATCGCAAGAAGCTGGCTCAGCCTATCACCGTGCAGGTTTGGAATC
  ACCGAGTCCTGAAGGATGAATTCCTGGGCCAGGTGCACCTGAAGACTGCCCCGGATGACCTGCAGGACCT
  CCACACCCTCCATCTCCAGGACCGCAGTAGCCGGCAGCCCAGTGACCTGCCAGGCATTGTAGCTGTGCGA
  GTCCTCTGCAGTGCCTCTCTCACGGCTGTC
  
  ACGCGTACGCGGCCGCTCGAGCAGAAACTCATCTCAGAAGAGGATCTGGCAGCAAATGATATCCTGGATT
  ACAAGGATGACGACGATAAGGTTTAA
  Protein Sequence

  >MR230759 representing NM_001301250
  Red=Cloning site Green=Tags(s)
  
  MFSCAKAYEDQNYSALKRACLRKKVLFEDPLFPATDDSLYYKGTPGPTVRWKRPKDICDDPRLFVDGISS
  HDLHQGQVGNCWFVAACSSLASRESLWQKVIPDWKEQEWNPEKPDSYAGIFHFNFWRFGEWVDVIVDDRL
  PTVNNQLIYCHSNSKNEFWCALVEKAYAKLAGCYQALDGGNTADALVDFTGGVSEPIDLTEGDLATDEAK
  RNQLFERVLKVHSRGGLISASIKAVTAADMEARLACGLVKGHAYAVTDVRKVRLGHGLLAFFKSEKLDMI
  RLRNPWGEREWTGPWSDTSEEWQKVSKSEREKMGVTVQDDGEFWMTFEDMCRYFTDIIKCRLINTSYLSI
  HKTWEEARLHGAWTRHEDPQQNRSGGCINHKDTFFQNPQYVFEVKKPEDEVLISIQQRPKRSTRREGKGE
  NLAIGFDIYKVEENRQYRMHSLQHKAASSIYINSRSVFLRTELPEGRYVIIPTTFEPGHTGEFLLRVFTD
  VPSNCRELRLDEPPRTCWSSLCGYPQQVAQVHVLGAAGLKDSPTGANSYVIIKCEGEKVRSAVQRGTSTP
  EYNVKGIFYRKKLAQPITVQVWNHRVLKDEFLGQVHLKTAPDDLQDLHTLHLQDRSSRQPSDLPGIVAVR
  VLCSASLTAV
  
  TRTRPLEQKLISEEDLAANDILDYKDDDDKV
• Restriction Sites: SgfI-MluI Cloning Scheme for this gene Plasmid Map
• ACCN: NM_001301250
• ORF Size: 1920 bp
• OTI Disclaimer: The molecular sequence of this clone aligns with the gene accession number as a point of reference only. However, individual transcript sequences of the same gene can differ through naturally occurring variations (e.g. polymorphisms), each with its own valid existence. This clone is substantially in agreement with the reference, but a complete review of all prevailing variants is recommended prior to use. More info
• OTI Annotation: This clone was engineered to express the complete ORF with an expression tag. Expression varies depending on the nature of the gene.

Reference Data

• RefSeq: NM_001301250.1, NP_001288179.1
• RefSeq Size: 4351
• RefSeq ORF: 1923
• Locus ID: 12337
• MW: 73 kDa
• Gene Summary: The protein encoded by this gene is a member of the calpain family of proteins. Unlike many members of the calpain gene family, this gene lacks a calmodulin-like domain, required for calcium binding. Mouse models for Huntington's disease displayed increased levels of the protein encoded by this gene. Alternative splicing results in multiple transcript variants. [provided by RefSeq, Jul 2014]