Numb (NM_001272055) Mouse Tagged ORF Clone

CAT#: MR230770

Numb (myc-DDK-tagged) - Mouse numb gene homolog (Drosophila) (Numb), transcript variant 3

Specifications

Product Data

• Type: Mouse Tagged ORF Clone
• Tag: Myc-DDK
• Symbol: Numb
• Synonyms: Nb
• Vector: pCMV6-Entry
• E. coli Selection: Kanamycin (25 ug/mL)
• Mammalian Cell Selection: Neomycin
• Sequence Data:
  ORF Nucleotide Sequence

  >MR230770 representing NM_001272055
  Red=Cloning site Blue=ORF Green=Tags(s)
  
  TTTTGTAATACGACTCACTATAGGGCGGCCGGGAATTCGTCGACTGGATCCGGTACCGAGGAGATCTGCC
  GCCGCGATCGCC
  
  ATGAACAAACTACGGCAAAGCTTCAGGAGAAAGAAAGACGTTTATGTCCCAGAGGCCAGCCGTCCACATC
  AGTGGCAGACAGATGAAGAAGGAGTCCGCACTGGAAAGTGTAGCTTCCCAGTTAAGTACCTCGGCCACGT
  AGAAGTTGATGAGTCAAGAGGAATGCACATCTGTGAAGATGCCGTAAAGAGATTGAAAGCTACGGGAAAG
  AAAGCAGTGAAGGCCGTTCTGTGGGTGTCAGCGGATGGGCTCAGAGTTGTGGACGAGAAAACTAAGGACC
  TCATAGTTGACCAGACAATAGAAAAAGTTTCCTTCTGTGCCCCAGATAGGAACTTTGACAGAGCCTTTTC
  TTACATATGTCGTGATGGCACCACTCGGCGATGGATCTGTCATTGTTTCATGGCTGTCAAAGACACGGGG
  GAAAGACTGAGCCACGCCGTGGGCTGTGCTTTTGCAGCCTGTTTAGAGCGTAAACAGAAGCGGGAGAAGG
  AGTGTGGCGTCACTGCTACTTTTGATGCCAGTAGAACCACTTTCACAAGAGAAGGATCATTCCGTGTCAC
  AACTGCCACTGAGCAAGCCGAAAGAGAGGAGATCATGAAACAGTTGCAAGATGCCAAGAAAGCTGAGACA
  GACAAGACAGTTGTTGGTCCATCAGTGGCTCCTGGCAACACTGCTCCATCCCCATCCTCTCCCACCTCTC
  CCACTCCGGATGGCACTGCATCTTCAGAGATGAACAATCCCCATGCTATCCCACGCCGGCATGCACCAAT
  TGAACAGCTTGCTCGTCAAGGCTCTTTCCGGGGATTTCCTGCTCTTAGCCAGAAGATGTCACCCTTTAAA
  CGCCAGCTGTCCCTACGCATCAATGAGTTGCCTTCCACTATGCAGAGGAAGACCGATTTCCCAATAAAAA
  ACACAGTGCCCGAGGTGGAAGGAGAGGCCGAGAGCATCAGCTCCTTGTGTTCCCAGATCACCAGTGCCTT
  CAGCACGCCCTCTGAGGACCCCTTCTCCTCCGCCCCAATGACCAAACCAGTGACATTGGTGGCACCACAG
  TCTCCTGTGTTACAAGCTAATGGCACTGACTCAGCCTCCCATGTGCTTACTGCTAAGCCAGCCAATACTG
  CTCTAGCACACGTAGCAATGCCTGTCCGTGAAACCAACCCCTGGGCCCATGTCCCTGATGCTGCTAACAA
  GGAAATTGCAGCCATACATCCGGGGACTGAGTGGGGTCAGTCTTCTGGTGCTGCCTCTCCAGGTCTCTTC
  CAGGCTGGTCACAGACGCACTCCCTCTGAAGCTGACCGCTGGTTAGAAGAAGTGTCAAAGAGTGTGCGGG
  CCCAGCAGCCTCAGGTCTCAGCTGCCCCTCTGCAGCCAGTTCTCCAGCCGCCTCCGCCCGCCGCCATTGC
  CCCTCCAGCACCTCCTTTCCAAGGACATGCCTTCCTCACGTCCCAGCCTGTGCCCGTGGGTGTGGTCCCA
  CCCCTACAACCAGCCTTTGTCCCTACCCAGTCCTACCCTGTGGCCAACGGGATGCCCTACCCAGCCTCTA
  ATGTGCCTGTAGTGGGCATCACCCCATCCCAGATGGTAGCCAATGTGTTTGGCACTGCAGGCCACCCTCA
  GACAACTCATCCACATCAGTCGCCAAGCCTGGCCAAGCAGCAGACATTCCCTCAATATGAGACAAGTAGT
  GCTACCACCAGTCCCTTCTTTAAGCCTCCTGCTCAGCACCTCAATGGTTCTGCAGCTTTCAATGGTGTAG
  ACAATGGTGGGCTAGCCTCAGGAAACAGGCATGCAGAAGTCCCTCCAGGCACCTGCCCAGTGGATCCTTT
  CGAAGCTCAGTGGGCCGCACTAGAAAGCAAGTCCAAGCAGCGTACAAATCCTTCTCCTACCAACCCTTTC
  TCCAGTGACTTACAGAAAACATTTGAAATAGAACTT
  
  ACGCGTACGCGGCCGCTCGAGCAGAAACTCATCTCAGAAGAGGATCTGGCAGCAAATGATATCCTGGATT
  ACAAGGATGACGACGATAAGGTTTAA
  Protein Sequence

  >MR230770 representing NM_001272055
  Red=Cloning site Green=Tags(s)
  
  MNKLRQSFRRKKDVYVPEASRPHQWQTDEEGVRTGKCSFPVKYLGHVEVDESRGMHICEDAVKRLKATGK
  KAVKAVLWVSADGLRVVDEKTKDLIVDQTIEKVSFCAPDRNFDRAFSYICRDGTTRRWICHCFMAVKDTG
  ERLSHAVGCAFAACLERKQKREKECGVTATFDASRTTFTREGSFRVTTATEQAEREEIMKQLQDAKKAET
  DKTVVGPSVAPGNTAPSPSSPTSPTPDGTASSEMNNPHAIPRRHAPIEQLARQGSFRGFPALSQKMSPFK
  RQLSLRINELPSTMQRKTDFPIKNTVPEVEGEAESISSLCSQITSAFSTPSEDPFSSAPMTKPVTLVAPQ
  SPVLQANGTDSASHVLTAKPANTALAHVAMPVRETNPWAHVPDAANKEIAAIHPGTEWGQSSGAASPGLF
  QAGHRRTPSEADRWLEEVSKSVRAQQPQVSAAPLQPVLQPPPPAAIAPPAPPFQGHAFLTSQPVPVGVVP
  PLQPAFVPTQSYPVANGMPYPASNVPVVGITPSQMVANVFGTAGHPQTTHPHQSPSLAKQQTFPQYETSS
  ATTSPFFKPPAQHLNGSAAFNGVDNGGLASGNRHAEVPPGTCPVDPFEAQWAALESKSKQRTNPSPTNPF
  SSDLQKTFEIEL
  
  TRTRPLEQKLISEEDLAANDILDYKDDDDKV
• Restriction Sites: SgfI-MluI Cloning Scheme for this gene Plasmid Map
• ACCN: NM_001272055
• ORF Size: 1926 bp
• OTI Disclaimer: The molecular sequence of this clone aligns with the gene accession number as a point of reference only. However, individual transcript sequences of the same gene can differ through naturally occurring variations (e.g. polymorphisms), each with its own valid existence. This clone is substantially in agreement with the reference, but a complete review of all prevailing variants is recommended prior to use. More info
• OTI Annotation: This clone was engineered to express the complete ORF with an expression tag. Expression varies depending on the nature of the gene.

Reference Data

• RefSeq: NM_001272055.1, NP_001258984.1
• RefSeq Size: 3349
• RefSeq ORF: 1929
• Locus ID: 18222
• MW: 69.9 kDa
• Gene Summary: This gene encodes a conserved protein that is distributed asymmetrically during cell division in the developing embryo. The encoded protein participates in cell fate decisions by interacting with the Notch receptor. Loss of function of this gene results in severe defects in neural development and loss of viability. Alternative splicing results in multiple transcript variants. [provided by RefSeq, Jan 2013]