Tle2 (NM_001252401) Mouse Tagged ORF Clone

CAT#: MR231018

Tle2 (myc-DDK-tagged) - Mouse transducin-like enhancer of split 2, homolog of Drosophila E(spl) (Tle2), transcript variant 2

Specifications

Product Data

• Type: Mouse Tagged ORF Clone
• Tag: Myc-DDK
• Symbol: Tle2
• Synonyms: Grg2; mKIAA4188
• Vector: pCMV6-Entry
• E. coli Selection: Kanamycin (25 ug/mL)
• Mammalian Cell Selection: Neomycin
• Sequence Data:
  ORF Nucleotide Sequence

  >MR231018 representing NM_001252401
  Red=Cloning site Blue=ORF Green=Tags(s)
  
  TTTTGTAATACGACTCACTATAGGGCGGCCGGGAATTCGTCGACTGGATCCGGTACCGAGGAGATCTGCC
  GCCGCGATCGCC
  
  ATGTACCCTCAGGGAAGGCACCCGACCCCGCTGCAGTCTGGCCAGCCTTTCAAGTTCTCAGTACTGGAAA
  TCTGTGACCGGATCAAAGAGGAATTCCAGTTTCTTCAAGCTCAGTACCACAGCCTCAAGCTAGAATGTGA
  GAAGCTGGCCAGCGAGAAGACAGAAATGCAAAGGCATTATGTGATGTACTACGAGATGTCCTACGGACTC
  AACATTGAGATGCATAAACAGGCTGAGATTGTGAAACGCCTCAGTGCGATCTGTGCCCAGATGGTCCCGT
  TCCTCACTCAGGAGCATCAGCAGCAGGTGCTCCAGGCTGTGGACCGAGCCAAGCAGCAGCAGAATCAGCT
  CCAGCCGCTGTCCCACGCACCCCCCGTGCCTCTCACCCCGCGCCCAGCCGGCCTGGTGGGTGCCGGGGCC
  ACTGGGCTGCTGGCCCTATCTGGGGCACTGGCTGCGCAGGCCCAGCTGGTGGCTGCCGTAAAGGAAGACC
  GTGTGGGTGTGGACGCCGAGGGGTCCAGAGTGGACAGAGCTGCCAGCAGGAGTTCTTCCCCGTCTCCCCC
  TGAGAGTCTGGTGGAAGAGGACCATCCCAGCAGCCGAGGCGGCAGTGGGAAACAGCAGAGAGCTGAAGAC
  AAGGATCTGTCAGGGCCTTATGACAGTGAGGAAGACAAGAGTGACTATAACCTGGTAGTGGATGAGGACC
  AACCGTCAGAGCCCCCCAGCCCTGTGACCACCCCTTGTGGGAAGGCGCCCCTCTGCATTCCTGCCCGCAG
  GGACCTCACAGACAGTCCAGCCTCCTTGGCCTCCAGTTTGGGCTCACCACTCCCCAGAAGCAAAGACATA
  GCCCTGAACGATCTTCCCACAGGCACTCCTGCCTCCAGGTCATGTGGTACCTCTCCACCCCAGGACTCGT
  CCACCCCGGGGCCCAGCTCAGCCAGTCACCTCTGCCAGCTGGCGGCTCAGCCGGCAGCACCCACAGACAG
  CATCGCCCTGAGGAGTCCCCTGACCTTGTCCAGCCCTTTCACCTCATCCTTCAGCCTGGGCTCCCACAGC
  ACCCTCAATGGGGACCTCTCCATGCCTGGCTCCTATGTCGGCCTCCACCTGTCCCCCCAGGTCAGCAGCT
  CTGTCGTGTATGGACGCTCACCTCTGATGGCATTTGAATCGCACCCCCATCTCCGAGGCTCGTCTGTCTC
  CTTGCCTGGCATCCCTGTGGCTAAGCCGGCTTACTCCTTCCACGTGTCTGCGGATGGCCAGATGCAGCCT
  GTGCCCTTCCCGTCTGATGCTCTGGTAGGCACAGGCATCCCTCGCCACGCAAGGCAGCTACACACGCTGG
  CCCACGGTGAGGTGGTGTGTGCCGTCACCATCAGCAGCTCCACACAGCACGTGTACACAGGCGGCAAAGG
  ATGCGTGAAGGTGTGGGACGTGGGCCAGCCGGGTAGCAAGACCCCTGTGGCACAGCTGGATTGCCTGAAC
  CGAGACAACTACATCCGCTCCTGCAAGCTGCTGCCCGACGGGCAGAGCCTGATTGTAGGTGGCGAGGCCA
  GTACCCTGTCCATTTGGGACCTGGCAGCACCCACACCACGGATCAAAGCAGAGCTGACTTCGTCTGCCCC
  GGCCTGCTATGCTCTGGCCGTCAGTCCGGACGCCAAGGTCTGCTTCTCCTGCTGCAGCGACGGTAACATC
  GTGGTCTGGGACCTGCAGAACCAGGCCATGGTCAGACAGTTCCAGGGCCACACGGACGGGGCCAGCTGCA
  TCGACATATCAGACTACGGGACCCGGCTGTGGACTGGGGGCCTGGACAACACTGTGCGCTGCTGGGACCT
  GCGCGAGGGCCGCCAGCTTCAGCAGCATGACTTCAGTTCCCAGATTTTCTCATTGGGCCACTGTCCCAAT
  CAGGACTGGTTGGCTGTGGGGATGGAGAGCAGCCACGTGGAGGTCCTGCATGTGCGCAAGCCTGAGAAGT
  ACCAGCTCCGCCTCCATGAGAGCTGCGTGCTGTCACTCAAGTTCGCTTCCTGTGGACGCTGGTTTGTGAG
  CACAGGCAAGGACAACCTGCTCAATGCCTGGAGGACACCCTATGGGGCCAGCATTTTTCAGTCCAAAGAA
  TCATCTTCTGTACTGAGCTGCGACATCTCCAGGAATAATAAGTACATCGTGACAGGCTCAGGGGACAAGA
  AGGCCACTGTGTATGAGGTGGTGTAC
  
  ACGCGTACGCGGCCGCTCGAGCAGAAACTCATCTCAGAAGAGGATCTGGCAGCAAATGATATCCTGGATT
  ACAAGGATGACGACGATAAGGTTTAA
  Protein Sequence

  >MR231018 representing NM_001252401
  Red=Cloning site Green=Tags(s)
  
  MYPQGRHPTPLQSGQPFKFSVLEICDRIKEEFQFLQAQYHSLKLECEKLASEKTEMQRHYVMYYEMSYGL
  NIEMHKQAEIVKRLSAICAQMVPFLTQEHQQQVLQAVDRAKQQQNQLQPLSHAPPVPLTPRPAGLVGAGA
  TGLLALSGALAAQAQLVAAVKEDRVGVDAEGSRVDRAASRSSSPSPPESLVEEDHPSSRGGSGKQQRAED
  KDLSGPYDSEEDKSDYNLVVDEDQPSEPPSPVTTPCGKAPLCIPARRDLTDSPASLASSLGSPLPRSKDI
  ALNDLPTGTPASRSCGTSPPQDSSTPGPSSASHLCQLAAQPAAPTDSIALRSPLTLSSPFTSSFSLGSHS
  TLNGDLSMPGSYVGLHLSPQVSSSVVYGRSPLMAFESHPHLRGSSVSLPGIPVAKPAYSFHVSADGQMQP
  VPFPSDALVGTGIPRHARQLHTLAHGEVVCAVTISSSTQHVYTGGKGCVKVWDVGQPGSKTPVAQLDCLN
  RDNYIRSCKLLPDGQSLIVGGEASTLSIWDLAAPTPRIKAELTSSAPACYALAVSPDAKVCFSCCSDGNI
  VVWDLQNQAMVRQFQGHTDGASCIDISDYGTRLWTGGLDNTVRCWDLREGRQLQQHDFSSQIFSLGHCPN
  QDWLAVGMESSHVEVLHVRKPEKYQLRLHESCVLSLKFASCGRWFVSTGKDNLLNAWRTPYGASIFQSKE
  SSSVLSCDISRNNKYIVTGSGDKKATVYEVVY
  
  TRTRPLEQKLISEEDLAANDILDYKDDDDKV
• Restriction Sites: SgfI-MluI Cloning Scheme for this gene Plasmid Map
• ACCN: NM_001252401
• ORF Size: 2196 bp
• OTI Disclaimer: The molecular sequence of this clone aligns with the gene accession number as a point of reference only. However, individual transcript sequences of the same gene can differ through naturally occurring variations (e.g. polymorphisms), each with its own valid existence. This clone is substantially in agreement with the reference, but a complete review of all prevailing variants is recommended prior to use. More info
• OTI Annotation: This clone was engineered to express the complete ORF with an expression tag. Expression varies depending on the nature of the gene.

Reference Data

• RefSeq: NM_001252401.1, NP_001239330.1
• RefSeq Size: 2721
• RefSeq ORF: 2199
• Locus ID: 21886
• MW: 79.4 kDa