App (NM_001198825) Mouse Tagged ORF Clone

CAT#: MR231020

App (myc-DDK-tagged) - Mouse amyloid beta (A4) precursor protein (App), transcript variant 5

Specifications

Product Data

• Type: Mouse Tagged ORF Clone
• Tag: Myc-DDK
• Symbol: App
• Synonyms: Abeta; Abpp; Adap; Ag; betaApp; Cvap; E030013M08Rik
• Vector: pCMV6-Entry
• E. coli Selection: Kanamycin (25 ug/mL)
• Mammalian Cell Selection: Neomycin
• Sequence Data:
  ORF Nucleotide Sequence

  >MR231020 representing NM_001198825
  Red=Cloning site Blue=ORF Green=Tags(s)
  
  TTTTGTAATACGACTCACTATAGGGCGGCCGGGAATTCGTCGACTGGATCCGGTACCGAGGAGATCTGCC
  GCCGCGATCGCC
  
  ATGCTGCCCAGCTTGGCACTGCTCCTGCTGGCCGCCTGGACGGTTCGGGCTCTGGAGGTACCCACTGATG
  GCAACGCCGGGCTGCTGGCAGAACCCCAGATCGCCATGTTCTGTGGTAAACTCAACATGCACATGAATGT
  GCAGAATGGAAAGTGGGAGTCAGACCCGTCAGGGACCAAAACCTGCATTGGCACCAAGGAGGGCATCTTG
  CAGTACTGCCAAGAGGTCTACCCTGAACTGCAGATCACAAACGTGGTGGAAGCCAACCAGCCAGTGACCA
  TCCAGAACTGGTGCAAGCGGGGCCGCAAGCAGTGCAAGACACACACCCACATCGTGATTCCTTACCGTTG
  CCTAGTTGGTGAGTTTGTGAGCGACGCCCTTCTCGTGCCCGACAAGTGCAAGTTCCTACACCAGGAGCGG
  ATGGATGTTTGTGAGACCCATCTTCACTGGCACACCGTCGCCAAAGAGACATGCAGCGAGAAGAGCACTA
  ACTTGCACGACTATGGCATGCTGCTGCCCTGCGGCATCGACAAGTTCCGAGGGGTAGAGTTTGTATGCTG
  CCCGTTGGCCGAGGAAAGCGACAGCGTGGATTCTGCGGATGCAGAGGAGGATGACTCTGATGTCTGGTGG
  GGTGGAGCGGACACAGACTACGCTGATGGCGGTGAAGACAAAGTAGTAGAAGTCGCCGAAGAGGAGGAAG
  TGGCTGATGTTGAGGAAGAGGAAGCTGATGATGATGAGGATGTGGAGGATGGGGACGAGGTGGAGGAGGA
  GGCCGAGGAGCCCTACGAAGAGGCCACCGAGAGAACAACCAGCACTGCCACCACCACCACAACCACCACT
  GAGTCCGTGGAGGAGGTGGTCCGAGAGGTGTGCTCTGAACAAGCCGAGACCGGGCCATGCCGCGCAATGA
  TCTCCCGCTGGTACTTTGATGTCACTGAAGGGAAGTGTGTCCCATTCTTTTACGGCGGATGTGGCGGCAA
  CAGGAACAACTTTGACACGGAAGAGTACTGCATGGCGGTGTGTGGCAGCGTGTTTCCCACGACAGCAGCC
  AGCACCCCCGACGCCGTCGACAAGTACCTGGAGACACCCGGGGACGAGAACGAGCATGCCCATTTCCAGA
  AAGCCAAAGAGAGGCTGGAAGCCAAGCACCGAGAGAGAATGTCCCAGGTCATGAGAGAATGGGAAGAGGC
  AGAGCGTCAAGCCAAGAACTTGCCCAAAGCTGACAAGAAGGCCGTTATCCAGCATTTCCAGGAGAAAGTG
  GAATCTCTGGAACAGGAAGCAGCCAATGAGAGACAGCAGCTTGTAGAGACACACATGGCCAGAGTTGAAG
  CCATGCTCAATGACCGCCGCCGCCTGGCCCTCGAGAATTACATCACTGCACTGCAGGCGGTGCCCCCAAG
  GCCTCATCATGTGTTCAACATGCTGAAGAAGTACGTCCGTGCGGAGCAGAAAGACAGACAGCACACCCTA
  AAGCATTTTGAACATGTGCGCATGGTGGACCCCAAGAAAGCTGCTCAGATCCGGTCCCAGGTTATGACAC
  ACCTCCGTGTGATCTACGAGCGCATGAACCAGTCTCTGTCCCTGCTCTACAATGTCCCTGCGGTGGCTGA
  GGAGATTCAAGATGAAGTCGATGAGCTGCTTCAGAAGGAGCAGAACTACTCCGACGATGTCTTGGCCAAC
  ATGATCAGTGAGCCCAGAATCAGCTACGGAAACGACGCTCTCATGCCTTCGCTGACGGAAACCAAGACCA
  CCGTGGAGCTCCTTCCCGTGAATGGGGAATTCAGCCTGGATGACCTCCAGCCGTGGCACCCTTTTGGGGT
  GGACTCTGTGCCAGCCAATACCGAAAATGAAGGTTCTGGGCTGACAAACATCAAGACGGAAGAGATCTCG
  GAAGTGAAGATGGATGCAGAATTCGGACATGATTCAGGATTTGAAGTCCGCCATCAAAAACTGGTGTTCT
  TTGCTGAAGATGTGGGTTCGAACAAAGGCGCCATCATCGGACTCATGGTGGGCGGCGTTGTCATAGCAAC
  CGTGATTGTCATCACCCTGGTGATGTTGAAGAAGAAACAGTACACATCCATCCATCATGGCGTGGTGGAG
  GTCGACGCCGCCGTGACCCCAGAGGAGCGCCATCTCTCCAAGATGCAGCAGAACGGATATGAGAATCCAA
  CTTACAAGTTCTTTGAGCAAATGCAGAAC
  
  ACGCGTACGCGGCCGCTCGAGCAGAAACTCATCTCAGAAGAGGATCTGGCAGCAAATGATATCCTGGATT
  ACAAGGATGACGACGATAAGGTTTAA
  Protein Sequence

  >MR231020 representing NM_001198825
  Red=Cloning site Green=Tags(s)
  
  MLPSLALLLLAAWTVRALEVPTDGNAGLLAEPQIAMFCGKLNMHMNVQNGKWESDPSGTKTCIGTKEGIL
  QYCQEVYPELQITNVVEANQPVTIQNWCKRGRKQCKTHTHIVIPYRCLVGEFVSDALLVPDKCKFLHQER
  MDVCETHLHWHTVAKETCSEKSTNLHDYGMLLPCGIDKFRGVEFVCCPLAEESDSVDSADAEEDDSDVWW
  GGADTDYADGGEDKVVEVAEEEEVADVEEEEADDDEDVEDGDEVEEEAEEPYEEATERTTSTATTTTTTT
  ESVEEVVREVCSEQAETGPCRAMISRWYFDVTEGKCVPFFYGGCGGNRNNFDTEEYCMAVCGSVFPTTAA
  STPDAVDKYLETPGDENEHAHFQKAKERLEAKHRERMSQVMREWEEAERQAKNLPKADKKAVIQHFQEKV
  ESLEQEAANERQQLVETHMARVEAMLNDRRRLALENYITALQAVPPRPHHVFNMLKKYVRAEQKDRQHTL
  KHFEHVRMVDPKKAAQIRSQVMTHLRVIYERMNQSLSLLYNVPAVAEEIQDEVDELLQKEQNYSDDVLAN
  MISEPRISYGNDALMPSLTETKTTVELLPVNGEFSLDDLQPWHPFGVDSVPANTENEGSGLTNIKTEEIS
  EVKMDAEFGHDSGFEVRHQKLVFFAEDVGSNKGAIIGLMVGGVVIATVIVITLVMLKKKQYTSIHHGVVE
  VDAAVTPEERHLSKMQQNGYENPTYKFFEQMQN
  
  TRTRPLEQKLISEEDLAANDILDYKDDDDKV
• Restriction Sites: SgfI-MluI Cloning Scheme for this gene Plasmid Map
• ACCN: NM_001198825
• ORF Size: 2199 bp
• OTI Disclaimer: The molecular sequence of this clone aligns with the gene accession number as a point of reference only. However, individual transcript sequences of the same gene can differ through naturally occurring variations (e.g. polymorphisms), each with its own valid existence. This clone is substantially in agreement with the reference, but a complete review of all prevailing variants is recommended prior to use. More info
• OTI Annotation: This clone was engineered to express the complete ORF with an expression tag. Expression varies depending on the nature of the gene.

Reference Data

• RefSeq: NM_001198825.1, NP_001185754.1
• RefSeq Size: 3266
• RefSeq ORF: 2202
• Locus ID: 11820
• MW: 83.2 kDa