Sema3a (NM_001243073) Mouse Tagged ORF Clone

CAT#: MR231120

Sema3a (myc-DDK-tagged) - Mouse sema domain, immunoglobulin domain (Ig), short basic domain, secreted, (semaphorin) 3A (Sema3a), transcript variant 3

Specifications

Product Data

• Type: Mouse Tagged ORF Clone
• Tag: Myc-DDK
• Symbol: Sema3a
• Synonyms: coll-1; Hsema-I; SEMA1; Semad; SemD
• Vector: pCMV6-Entry
• E. coli Selection: Kanamycin (25 ug/mL)
• Mammalian Cell Selection: Neomycin
• Sequence Data:
  ORF Nucleotide Sequence

  >MR231120 ORF sequence
  Red=Cloning site Blue=ORF Green=Tags(s)
  
  TTTTGTAATACGACTCACTATAGGGCGGCCGGGAATTCGTCGACTGGATCCGGTACCGAGGAGATCTGCC
  GCCGCGATCGCC
  
  ATGGGCTGGTTCACTGGGATTGCCTGTCTTTTCTGGGGTGTATTACTTACAGCCAGAGCAAACTATGCAA
  ACGGAAAGAACAATGTGCCAAGACTGAAATTATCGTACAAAGAAATGTTGGAATCCAACAATGTGATCAC
  TTTTAATGGCTTGGCCAACAGCTCCAGTTACCACACCTTCCTTCTGGATGAAGAACGGAGTAGACTATAT
  GTTGGAGCAAAAGATCATATATTTTCATTCAACTTGGTGAACATTAAAGATTTTCAAAAGATTGTGTGGC
  CAGTATCTTACACAAGGAGAGATGAATGCAAATGGGCTGGAAAAGATATCCTGAAAGAATGTGCCAATTT
  CATCAAGGTCCTGGAGGCTTATAATCAGACTCACTTGTATGCCTGTGGAACTGGGGCTTTCCATCCAATC
  TGCACCTATATTGAAGTTGGACATCATCCTGAGGACAACATTTTTAAGCTGCAGGACTCACATTTTGAAA
  ACGGTCGTGGGAAGAGCCCTTATGATCCCAAACTACTGACTGCCTCTCTTCTAATAGACGGTGAGTTGTA
  CTCTGGAACTGCTGCGGACTTCATGGGACGGGACTTCGCTATCTTCAGAACACTGGGGCACCATCACCCC
  ATCAGGACGGAGCAGCATGACTCCCGGTGGCTCAATGATCCTAGATTCATCAGTGCCCATCTCATCCCAG
  AGAGTGACAACCCTGAAGATGACAAAGTATATTTTTTCTTCCGAGAAAATGCAATAGACGGAGAACACTC
  TGGAAAAGCCACTCATGCTAGAATAGGTCAGATATGCAAGAATGACTTTGGTGGACACAGAAGTCTTGTG
  AATAAATGGACAACATTCCTAAAAGCACGCCTGATTTGCTCTGTGCCCGGTCCCAATGGCATTGACACCC
  ATTTTGATGAATTGCAGGATGTATTCCTAATGAACTCTAAAGATCCTAAAAATCCGATCGTCTATGGAGT
  GTTCACAACATCAAGCAACATCTTTAAGGGATCTGCTGTGTGCATGTACAGCATGAGTGATGTAAGAAGG
  GTGTTCCTTGGTCCATATGCTCACAGAGATGGTCCCAACTATCAGTGGGTGCCTTACCAAGGAAGAGTCC
  CTTATCCACGGCCAGGAACTTGTCCCAGTAAAACATTTGGCGGATTTGACTCCACAAAGGACCTTCCTGA
  TGATGTCATAACTTTTGCAAGAAGTCATCCAGCCATGTACAACCCAGTGTTTCCTATAAATAATCGCCCG
  ATCATGATCAAAACAGATGTAAATTATCAGTTCACACAAATTGTTGTAGACCGAGTGGATGCAGAAGATG
  GCCAGTATGATGTTATGTTCATCGGAACAGATGTTGGAACCGTTCTTAAAGTGGTTTCAGTCCCCAAGGA
  GACTTGGCATGACCTAGAAGAAGTTCTTCTGGAAGAAATGACCGTCTTCCGGGAACCAACAACTATTTCG
  GCAATGGAGCTTTCTACTAAACAGCAACAGCTGTACATTGGCTCAACTGCGGGAGTGGCACAGCTTCCTC
  TACACCGCTGTGACATCTATGGCAAAGCCTGTGCAGAATGCTGCCTCGCTCGGGACCCTTACTGTGCCTG
  GGATGGGTCCTCATGCTCACGCTATTTTCCTACTGCAAAGAGGCGCACAAGACGACAAGATATAAGGAAT
  GGAGACCCACTGACTCACTGCTCTGACTTGCAGCACCATGATAATCATCATGGGCCCAGCCTTGAAGAGA
  GAATCATCTATGGAGTGGAAAACAGTAGTACATTCTTGGAATGCAGTCCGAAGTCACAGAGAGCCTTGGT
  ATATTGGCAATTTCAGAGGAGAAATGAAGATCGAAAAGAGGAGATCAGAATGGGTGATCATATCATCAGG
  ACAGAACAAGGGCTCCTGCTCCGTAGCCTGCAGAAGAAGGATTCAGGCAATTACCTGTGTCACGCTGTGG
  AACACGGATTCATGCAAACTCTTCTTAAGGTAACCCTGGAAGTCATTGACACAGAACATTTGGAAGAACT
  TCTTCATAAAGATGACGATGGAGATGGCTCTAAGATAAAAGAAATGTCGAGCAGCATGACGCCCAGCCAG
  AAAGTCTGGTACCGAGACTTCATGCAGCTCATTAACCACCCCAACCTGAACACGATGGATGAGTTCTGTG
  AACAAGTGTGGAAAAGGGACCGAAAGCAACGCCGACAAAGGCCGGGGCACTCTCAAGGGAGCAGCAACAA
  GTGGAAGCACATGCAAGAGAGCAAGAAAGGTAGAAACAGGAGGACCCACGAGTTTGAGCGGGCACCCAGA
  AGTGTC
  
  ACGCGTACGCGGCCGCTCGAGCAGAAACTCATCTCAGAAGAGGATCTGGCAGCAAATGATATCCTGGATT
  ACAAGGATGACGACGATAAGGTTTAA
  Protein Sequence

  >MR231120 protein sequence
  Red=Cloning site Green=Tags(s)
  
  MGWFTGIACLFWGVLLTARANYANGKNNVPRLKLSYKEMLESNNVITFNGLANSSSYHTFLLDEERSRLY
  VGAKDHIFSFNLVNIKDFQKIVWPVSYTRRDECKWAGKDILKECANFIKVLEAYNQTHLYACGTGAFHPI
  CTYIEVGHHPEDNIFKLQDSHFENGRGKSPYDPKLLTASLLIDGELYSGTAADFMGRDFAIFRTLGHHHP
  IRTEQHDSRWLNDPRFISAHLIPESDNPEDDKVYFFFRENAIDGEHSGKATHARIGQICKNDFGGHRSLV
  NKWTTFLKARLICSVPGPNGIDTHFDELQDVFLMNSKDPKNPIVYGVFTTSSNIFKGSAVCMYSMSDVRR
  VFLGPYAHRDGPNYQWVPYQGRVPYPRPGTCPSKTFGGFDSTKDLPDDVITFARSHPAMYNPVFPINNRP
  IMIKTDVNYQFTQIVVDRVDAEDGQYDVMFIGTDVGTVLKVVSVPKETWHDLEEVLLEEMTVFREPTTIS
  AMELSTKQQQLYIGSTAGVAQLPLHRCDIYGKACAECCLARDPYCAWDGSSCSRYFPTAKRRTRRQDIRN
  GDPLTHCSDLQHHDNHHGPSLEERIIYGVENSSTFLECSPKSQRALVYWQFQRRNEDRKEEIRMGDHIIR
  TEQGLLLRSLQKKDSGNYLCHAVEHGFMQTLLKVTLEVIDTEHLEELLHKDDDGDGSKIKEMSSSMTPSQ
  KVWYRDFMQLINHPNLNTMDEFCEQVWKRDRKQRRQRPGHSQGSSNKWKHMQESKKGRNRRTHEFERAPR
  SV
  
  TRTRPLEQKLISEEDLAANDILDYKDDDDKV
• Restriction Sites: SgfI-MluI Cloning Scheme for this gene Plasmid Map
• ACCN: NM_001243073
• ORF Size: 2316 bp
• OTI Disclaimer: The molecular sequence of this clone aligns with the gene accession number as a point of reference only. However, individual transcript sequences of the same gene can differ through naturally occurring variations (e.g. polymorphisms), each with its own valid existence. This clone is substantially in agreement with the reference, but a complete review of all prevailing variants is recommended prior to use. More info
• OTI Annotation: This clone was engineered to express the complete ORF with an expression tag. Expression varies depending on the nature of the gene.
• Product Components: The ORF clone is ion-exchange column purified, transfection-ready dried plasmid DNA, and shipped with 2 vector sequencing primers.
• Reconstitution: 1. Centrifuge at 5,000xg for 5min.
  2. Carefully open the tube and add 100ul of sterile water to dissolve the DNA.
  3. Close the tube and incubate for 10 minutes at room temperature.
  4. Briefly vortex the tube and then do a quick spin (less than 5000xg) to concentrate the liquid at the bottom.
  5. Store the suspended plasmid at -20°C. The DNA is stable for at least one year from date of shipping when stored at -20°C.

Reference Data

• RefSeq: NM_001243073.1, NP_001230002.1
• RefSeq Size: 6422 bp
• RefSeq ORF: 2319 bp
• Locus ID: 20346
• MW: 88.8 kDa