Cdc27 (NM_001285988) Mouse Tagged ORF Clone

CAT#: MR231224

Cdc27 (myc-DDK-tagged) - Mouse cell division cycle 27 (Cdc27), transcript variant 1

Specifications

Product Data

• Type: Mouse Tagged ORF Clone
• Tag: Myc-DDK
• Symbol: Cdc27
• Synonyms: AI452358; APC3; BC023187
• Vector: pCMV6-Entry
• E. coli Selection: Kanamycin (25 ug/mL)
• Mammalian Cell Selection: Neomycin
• Sequence Data:
  ORF Nucleotide Sequence

  >MR231224 representing NM_001285988
  Red=Cloning site Blue=ORF Green=Tags(s)
  
  TTTTGTAATACGACTCACTATAGGGCGGCCGGGAATTCGTCGACTGGATCCGGTACCGAGGAGATCTGCC
  GCCGCGATCGCC
  
  ATGACGGTGCTGCAGGAACCTGTCCAGGCTGCTATATGGCAAGCGCTAAACCACTATGCTTACCGAGATG
  CAGTTTTCCTCGCAGAACGACTATATGCAGAAGTACATTCAGAAGAAGCCTTGTTTTTACTGGCAACCTG
  TTACTACCGCTCAGGAAAGGCTTATAAAGCATATAGACTCTTGAAAGGACACAGTTGTACCACCCCACAG
  TGTAAATACCTGCTTGCAAAATGTTGTGTTGACCTCAGCAAGCTTGCAGAAGGGGAACAGATCTTATCTG
  GTGGAGTGTTTAATAAGCAGAAAAGCCATGACGACCTTGTCACTGAGTTTGGAGATTCAGCTTGCTTCAC
  TCTTTCCTTGTTGGGACATGTGTATTGCAAGACAGATCGGCTTGCCAAAGGGTCAGAATGTTACCAAAAG
  AGCCTTAGTTTAAATCCTTTCCTCTGGTCTCCCTTTGAATCGTTATGTGAAATAGGTGAGAAGCCAGATC
  CTGACCAAACATTTAAATTAACATCTCTACAGAATTTTAGCAGTTGTCTTCCCAACACTTGTACAACTCT
  AGTATCTAATCACAGTTTATCTCACAGACAGCCTGAGACAGTCCTTACAGAAACACCCCAAGACACAATT
  GAATTAAACAGACTGAATTTAGAATCTTCCAATTCAAAGTACTCCTTGAATACAGATTCCTCCGTGTCTT
  ACATTGATTCAACTGTAATTTCACCAGATAACGTCCCCCTTGGACCTGGTACTGCCATATTATCTAAACA
  GGTTCAAAATAAACCAAAAACTGGTCGAAGTTTATTAGGAGGACCAACTGCTCTTAGCCCATTAACCCCA
  AGTTTTGGGATTTTGCCATTAGAAACCCCAAGTCCTGGAGATGGATCCTATTTACAAAACTACACTAATA
  CACCTTCTGTAATTGATGTGGCACCCACCGGAGCACCTACAAAAAAGACTTTTTGTGTTTTACAGTCTGT
  TGCAAGAATGGGCCAAACTGGAACAAAGTCTGTCTTCTCACAGAGTGGAAATAGTCGAGAGGTCACACCA
  GTACTTGTTGCACAAACACAAAGTTCTGGCCCACAAACAAGTACAACACCTCAGGTATTGAGCCCCACTA
  TCACATCTCCCCCAAACGCATTGCCTCGGAGAAGTTCCCGCCTTTTCACTAGTGACAGTTCTACAACCAA
  GGAGAATAGCAAAAAGTTAAAAATGAAGTTTCCACCTAAAATCCCTAACAGAAAAACAAAAAGTAAAACT
  AATAAAGGAGGACTAACTCAGCCCAGCATAAACGATAGTCTGGAAATTACAAAACTGGACTCCTCTATCA
  TTTCAGAAGGGAAAATAACCACAGTCACACCTCAGATCCAGGCATTTAACCTACAAAAGGCAGCAGCAGG
  TTTGATGAGCCTTCTTCGTGAAATGGGAAAAGGTTATTTAGCTTTGTGTTCATACAACTGCAAAGAAGCT
  ATCAATATTTTGAGCCATCTACCTTCTCATCACTACAGTACTGGTTGGGTCCTATGCCAGATTGGACGGG
  CTTATTTTGAACTTTCAGAATACATGCAGGCTGAAAGAATATTCTCAGAGGTTAGAAGGATTGAGAGTTT
  CAGAGTTGAAGGAATGGAGATCTACTCTACAACACTCTGGCATCTTCAGAAAGACGTTGCTCTTTCAGTT
  CTTTCAAAAGATTTAACAGACATGGATAAGAATTCACCAGAGGCCTGGTGTGCTGCAGGGAATTGTTTCA
  GTCTACAACGAGAGCATGATATAGCAATTAAATTCTTCCAAAGAGCTATCCAGGTCGATCCAAATTACGC
  TTACGCCTATACTCTATTAGGACATGAGTTTGTGTTAACTGAAGAACTAGATAAAGCATTAGCATGTTTT
  CGAAATGCTATAAGAGTAAATCCCAGACATTACAATGCATGGTATGGTTTAGGAATGATTTATTACAAGC
  AAGAGAAGTTCAGCCTCGCAGAAATGCATTTCCAGAAAGCACTTGATATCAACCCTCAGAGTTCAGTTTT
  ACTTTGCCACATTGGAGTAGTTCAGCATGCACTAAAGAAGTCTGAGAAGGCTTTGGATACCCTAAACAAA
  GCCATTGTTATCGATCCCAAGAACCCTCTATGCAAATTTCACAGAGCCTCGGTTTTATTTGCAAATGAAA
  AATACAAGTCTGCTTTACAAGAACTCGAGGAACTGAAGCAGATTGTCCCCAAAGAGTCCCTCGTTTACTT
  CCTAATAGGAAAGGTTTACAAGAAGTTGGGTCAAACTCATCTCGCCTTGATGAATTTCTCTTGGGCTATG
  GATTTAGATCCTAAAGGAGCCAATAATCAGATTAAAGAGGCAATTGACAAGCGCTACCTTCCAGATGATG
  AGGAGCCAATAACCCAGGAGGAACAGATCATGGGGACAGATGAATCCCAGGAGAGCAGCATGACAGATGC
  AGATGACACACAACTTCATGCGGCGGAAAGTGACGAATTT
  
  ACGCGTACGCGGCCGCTCGAGCAGAAACTCATCTCAGAAGAGGATCTGGCAGCAAATGATATCCTGGATT
  ACAAGGATGACGACGATAAGGTTTAA
  Protein Sequence

  >MR231224 representing NM_001285988
  Red=Cloning site Green=Tags(s)
  
  MTVLQEPVQAAIWQALNHYAYRDAVFLAERLYAEVHSEEALFLLATCYYRSGKAYKAYRLLKGHSCTTPQ
  CKYLLAKCCVDLSKLAEGEQILSGGVFNKQKSHDDLVTEFGDSACFTLSLLGHVYCKTDRLAKGSECYQK
  SLSLNPFLWSPFESLCEIGEKPDPDQTFKLTSLQNFSSCLPNTCTTLVSNHSLSHRQPETVLTETPQDTI
  ELNRLNLESSNSKYSLNTDSSVSYIDSTVISPDNVPLGPGTAILSKQVQNKPKTGRSLLGGPTALSPLTP
  SFGILPLETPSPGDGSYLQNYTNTPSVIDVAPTGAPTKKTFCVLQSVARMGQTGTKSVFSQSGNSREVTP
  VLVAQTQSSGPQTSTTPQVLSPTITSPPNALPRRSSRLFTSDSSTTKENSKKLKMKFPPKIPNRKTKSKT
  NKGGLTQPSINDSLEITKLDSSIISEGKITTVTPQIQAFNLQKAAAGLMSLLREMGKGYLALCSYNCKEA
  INILSHLPSHHYSTGWVLCQIGRAYFELSEYMQAERIFSEVRRIESFRVEGMEIYSTTLWHLQKDVALSV
  LSKDLTDMDKNSPEAWCAAGNCFSLQREHDIAIKFFQRAIQVDPNYAYAYTLLGHEFVLTEELDKALACF
  RNAIRVNPRHYNAWYGLGMIYYKQEKFSLAEMHFQKALDINPQSSVLLCHIGVVQHALKKSEKALDTLNK
  AIVIDPKNPLCKFHRASVLFANEKYKSALQELEELKQIVPKESLVYFLIGKVYKKLGQTHLALMNFSWAM
  DLDPKGANNQIKEAIDKRYLPDDEEPITQEEQIMGTDESQESSMTDADDTQLHAAESDEF
  
  TRTRPLEQKLISEEDLAANDILDYKDDDDKV
• Restriction Sites: SgfI-MluI Cloning Scheme for this gene Plasmid Map
• ACCN: NM_001285988
• ORF Size: 2490 bp
• OTI Disclaimer: The molecular sequence of this clone aligns with the gene accession number as a point of reference only. However, individual transcript sequences of the same gene can differ through naturally occurring variations (e.g. polymorphisms), each with its own valid existence. This clone is substantially in agreement with the reference, but a complete review of all prevailing variants is recommended prior to use. More info
• OTI Annotation: This clone was engineered to express the complete ORF with an expression tag. Expression varies depending on the nature of the gene.

Reference Data

• RefSeq: NM_001285988.1, NP_001272917.1
• RefSeq Size: 5798
• RefSeq ORF: 2493
• Locus ID: 217232
• MW: 92.9 kDa