Dnm1 (NM_001301737) Mouse Tagged ORF Clone

CAT#: MR231256

Dnm1 (myc-DDK-tagged) - Mouse dynamin 1 (Dnm1), transcript variant 2

Specifications

Product Data

• Type: Mouse Tagged ORF Clone
• Tag: Myc-DDK
• Symbol: Dnm1
• Synonyms: AI838169; Dnm; Ftfl; mKIAA4093
• Vector: pCMV6-Entry
• E. coli Selection: Kanamycin (25 ug/mL)
• Mammalian Cell Selection: Neomycin
• Sequence Data:
  ORF Nucleotide Sequence

  >MR231256 representing NM_001301737
  Red=Cloning site Blue=ORF Green=Tags(s)
  
  TTTTGTAATACGACTCACTATAGGGCGGCCGGGAATTCGTCGACTGGATCCGGTACCGAGGAGATCTGCC
  GCCGCGATCGCC
  
  ATGGGCAACCGCGGCATGGAAGACCTCATCCCGCTGGTTAACCGGTTACAGGACGCCTTCTCCGCCATCG
  GCCAGAACGCGGACCTCGACCTGCCGCAGATCGCCGTGGTAGGCGGCCAGAGCGCCGGCAAGAGCTCGGT
  GCTGGAGAATTTCGTGGGCAGGGACTTCTTGCCCCGAGGATCTGGCATCGTCACCCGGCGTCCCCTGGTC
  CTGCAGCTGGTTAATTCTACCACAGAATATGCCGAGTTCCTGCACTGCAAGGGGAAGAAATTCACCGACT
  TCGAGGAGGTGCGCCTGGAGATCGAGGCTGAGACCGATCGAGTCACCGGCACCAACAAGGGCATTTCGCC
  AGTGCCCATCAACCTGCGGGTCTACTCGCCCCATGTGCTGAACCTGACTCTAGTGGACCTGCCAGGAATG
  ACCAAGGTCCCAGTTGGGGACCAACCTCCTGATATCGAGTTCCAGATCCGGGACATGCTTATGCAGTTCG
  TCACTAAGGAGAACTGCCTTATCCTGGCTGTGTCCCCTGCCAACTCGGATTTGGCCAACTCTGATGCCCT
  CAAGATCGCTAAGGAGGTGGACCCCCAGGGTCAGCGCACCATTGGGGTCATCACCAAGTTGGACCTGATG
  GACGAGGGCACAGATGCGCGGGACGTGCTAGAGAACAAGCTGCTCCCTTTGCGCAGAGGTTACATCGGCG
  TGGTGAACCGGAGCCAGAAGGACATAGACGGCAAGAAGGACATCACAGCCGCCTTGGCTGCAGAGCGCAA
  ATTCTTCCTCTCTCACCCATCCTACCGCCACTTGGCTGACCGCATGGGCACACCCTACCTGCAGAAGGTC
  CTCAATCAGCAATTGACCAACCACATCCGGGACACACTGCCGGGACTTCGGAACAAGCTGCAGAGCCAGC
  TGCTGTCCATTGAGAAGGAGGTGGACGAGTACAAGAACTTCCGACCGGATGACCCAGCGCGCAAGACCAA
  GGCCCTGCTGCAGATGGTCCAGCAGTTTGCAGTGGACTTCGAGAAGCGCATCGAGGGTTCTGGAGACCAG
  ATTGACACTTACGAGCTGTCAGGTGGAGCCCGCATTAACCGGATCTTCCATGAACGCTTCCCCTTTGAGC
  TGGTTAAGATGGAGTTTGATGAGAAGGAACTGCGAAGGGAGATCAGCTATGCTATCAAAAATATCCATGG
  CATCAGGACGGGCCTCTTCACACCTGACCTCGCTTTTGAAGCCACAGTGAAAAAGCAGGTGCAGAAGCTC
  AAAGAGCCCAGTATCAAGTGTGTGGACATGGTAGTCAGTGAACTCACGTCCACCATCAGAAAGTGTAGTG
  AAAAGCTGCAGCAATACCCGCGTCTGCGGGAGGAGATGGAGCGAATTGTGACCACCCACATCCGGGAACG
  TGAGGGCCGCACCAAGGAGCAGGTCATGCTTCTCATCGACATTGAGCTGGCTTACATGAATACCAACCAC
  GAAGACTTCATAGGCTTTGCCAATGCTCAGCAGAGAAGCAACCAGATGAACAAGAAGAAGACTTCAGGGA
  ACCAGGATGAGATTCTGGTCATTCGAAAGGGGTGGTTGACCATCAACAACATCGGCATCATGAAGGGAGG
  CTCCAAGGAGTACTGGTTTGTGCTGACTGCTGAGAATCTGTCCTGGTACAAGGATGATGAGGAGAAAGAG
  AAGAAGTACATGCTGTCTGTGGACAATCTGAAGCTGCGTGATGTGGAGAAGGGCTTCATGTCAAGCAAGC
  ATATTTTTGCCCTCTTCAACACAGAGCAGAGGAATGTCTACAAGGATTACCGGCAGCTGGAACTGGCCTG
  TGAGACACAGGAGGAGGTGGACAGTTGGAAGGCTTCCTTCCTGAGGGCTGGCGTGTACCCTGAGCGTGTT
  GGGGACAAAGAGAAAGCCAGTGAGACCGAGGAGAACGGCTCTGACAGCTTCATGCACTCGATGGACCCTC
  AGCTGGAGCGCCAGGTGGAGACCATCCGGAACCTGGTAGACTCGTACATGGCCATTGTCAACAAGACTGT
  GCGGGACCTCATGCCCAAGACCATCATGCACCTCATGATCAACAACACCAAGGAGTTTATCTTCTCTGAG
  CTGCTGGCCAACCTGTACTCTTGCGGGGACCAGAACACACTGATGGAAGAATCGGCCGAGCAGGCTCAGC
  GGCGCGACGAGATGCTGCGCATGTACCACGCACTGAAGGAGGCGCTCAGTATTATCGGCGACATCAACAC
  GACCACCGTCAGCACGCCCATGCCCCCGCCCGTGGACGACTCCTGGCTGCAGGTGCAGAGCGTACCGGCC
  GGACGCAGATCGCCCACGTCCAGCCCCACGCCGCAGCGCCGAGCCCCCGCCGTGCCCCCAGCCCGGCCCG
  GATCGCGGGGCCCTGCTCCTGGGCCTCCGCCTGCTGGATCCGCCCTGGGGGGGGCGCCCCCCGTGCCCTC
  CAGGCCGGGGGCTTCCCCTGACCCCTTTGGCCCCCCTCCCCAGGTGCCCTCGCGCCCCAACCGCGCCCCG
  CCTGGGGTCCCCAGAATCACTATCAGTGACCCC
  
  ACGCGTACGCGGCCGCTCGAGCAGAAACTCATCTCAGAAGAGGATCTGGCAGCAAATGATATCCTGGATT
  ACAAGGATGACGACGATAAGGTTTAA
  Protein Sequence

  >MR231256 representing NM_001301737
  Red=Cloning site Green=Tags(s)
  
  MGNRGMEDLIPLVNRLQDAFSAIGQNADLDLPQIAVVGGQSAGKSSVLENFVGRDFLPRGSGIVTRRPLV
  LQLVNSTTEYAEFLHCKGKKFTDFEEVRLEIEAETDRVTGTNKGISPVPINLRVYSPHVLNLTLVDLPGM
  TKVPVGDQPPDIEFQIRDMLMQFVTKENCLILAVSPANSDLANSDALKIAKEVDPQGQRTIGVITKLDLM
  DEGTDARDVLENKLLPLRRGYIGVVNRSQKDIDGKKDITAALAAERKFFLSHPSYRHLADRMGTPYLQKV
  LNQQLTNHIRDTLPGLRNKLQSQLLSIEKEVDEYKNFRPDDPARKTKALLQMVQQFAVDFEKRIEGSGDQ
  IDTYELSGGARINRIFHERFPFELVKMEFDEKELRREISYAIKNIHGIRTGLFTPDLAFEATVKKQVQKL
  KEPSIKCVDMVVSELTSTIRKCSEKLQQYPRLREEMERIVTTHIREREGRTKEQVMLLIDIELAYMNTNH
  EDFIGFANAQQRSNQMNKKKTSGNQDEILVIRKGWLTINNIGIMKGGSKEYWFVLTAENLSWYKDDEEKE
  KKYMLSVDNLKLRDVEKGFMSSKHIFALFNTEQRNVYKDYRQLELACETQEEVDSWKASFLRAGVYPERV
  GDKEKASETEENGSDSFMHSMDPQLERQVETIRNLVDSYMAIVNKTVRDLMPKTIMHLMINNTKEFIFSE
  LLANLYSCGDQNTLMEESAEQAQRRDEMLRMYHALKEALSIIGDINTTTVSTPMPPPVDDSWLQVQSVPA
  GRRSPTSSPTPQRRAPAVPPARPGSRGPAPGPPPAGSALGGAPPVPSRPGASPDPFGPPPQVPSRPNRAP
  PGVPRITISDP
  
  TRTRPLEQKLISEEDLAANDILDYKDDDDKV
• Restriction Sites: SgfI-MluI Cloning Scheme for this gene Plasmid Map
• ACCN: NM_001301737
• ORF Size: 2553 bp
• OTI Disclaimer: The molecular sequence of this clone aligns with the gene accession number as a point of reference only. However, individual transcript sequences of the same gene can differ through naturally occurring variations (e.g. polymorphisms), each with its own valid existence. This clone is substantially in agreement with the reference, but a complete review of all prevailing variants is recommended prior to use. More info
• OTI Annotation: This clone was engineered to express the complete ORF with an expression tag. Expression varies depending on the nature of the gene.

Reference Data

• RefSeq: NM_001301737.1, NP_001288666.1
• RefSeq Size: 3295
• RefSeq ORF: 2556
• Locus ID: 13429
• MW: 96.4 kDa
• Gene Summary: This gene encodes a member of the dynamin subfamily of GTP-binding proteins. The encoded protein is a GTPase which is required for membrane recycling, including vesicle endocytosis in neurons. It may also be involved in cellular fission via association with microtubules and actin filaments. Mutations in this gene have been shown to cause seizures. Alternative splicing results in multiple transcript variants. [provided by RefSeq, Sep 2014]