Cd22 (NM_009845) Mouse Tagged ORF Clone

CAT#: MR231289

Cd22 (myc-DDK-tagged) - Mouse CD22 antigen (Cd22), transcript variant 2

Specifications

Product Data

• Type: Mouse Tagged ORF Clone
• Tag: Myc-DDK
• Symbol: Cd22
• Synonyms: A530093D23; Lyb-8; Lyb8
• Vector: pCMV6-Entry
• E. coli Selection: Kanamycin (25 ug/mL)
• Mammalian Cell Selection: Neomycin
• Sequence Data:
  ORF Nucleotide Sequence

  >MR231289 representing NM_009845
  Red=Cloning site Blue=ORF Green=Tags(s)
  
  TTTTGTAATACGACTCACTATAGGGCGGCCGGGAATTCGTCGACTGGATCCGGTACCGAGGAGATCTGCC
  GCCGCGATCGCC
  
  ATGCGCGTCCATTACCTGTGGCTCCTCTTGATCCTAGGACACGTGGCTTCGGCTCGGTACAGCTCAGCAA
  ATGATTGGACCGTTGACCATCCCCAAACCCTCTTTGCCTGGGAGGGAGCCTGCATCAGGATTCCTTGCAA
  GTACAAAACTCCACTACCCAAGGCACGTCTGGACAACATCCTCCTTTTTCAGAACTATGAGTTTGACAAG
  GCCACCAAGAAATTCACAGGAACTGTCCTGTACAACGCCACAAAGACTGAGAAGGACCCAGAGTCTGAGC
  TGTACCTTTCTAAGCAAGGGAGAGTAACATTTCTGGGGAACAGAATAGACAATTGTACCCTGAAAATCCA
  CCCGATACGTGCCAATGACAGTGGGAATCTGGGGTTGAGGATGACCGCAGGGACTGAACGATGGATGGAG
  CCCATTCACCTCAATGTCTCGGAGAAACCGTTCCAACCTTACATCCAGATGCCATCAGAAATCCGGGAAT
  CCCAAAGTGTCACTCTGACCTGTGGACTGAATTTCTCCTGCTTTGGGTATGACATCCTCTTGAAGTGGTT
  CCTAGAAGATTCTGAGATCACCTCCATCACCTCTTCTGTCACCTCCATCACCTCTTCTGTCACCTCCTCC
  ATTAAGAATGTCTATACAGAGAGCAAGCTCACCTTCCAACCAAAGTGGACGGACCACGGAAAGAGTGTGA
  AGTGCCAAGTCCAGCACTCCTCCAAAGTGCTCTCAGAGTGCACTGTGCATCTGGATGTTAAGTATACCCC
  GAAGCTGGAGATCAAGGTCAATCCCACAGAGGTGGAAAAGAACAATTCTGTGACCATGACATGCCGGGTT
  AACAGCAGCAACCCGAAACTCAGGACCGTGGCGGTGTCTTGGTTCAAGGATGGGCGCCCCCTAGAGGATC
  AGGAACTGGAACAGGAACAACAGATGTCCAAGCTAATTCTGCATTCAGTGACCAAGGACATGAGAGGGAA
  ATACCGGTGCCAGGCTTCCAACGACATAGGCCCAGGAGAGTCGGAAGAAGTGGAACTCACGGTGCACTAT
  GCTCCAGAACCCTCCAGGGTTCACATCTACCCTTCCCCCGCTGAAGAGGGACAGTCAGTAGAGCTGATTT
  GTGAGTCACTGGCCAGTCCAAGTGCAACAAACTACACCTGGTATCACAACAGGAAACCTATACCTGGAGA
  CACCCAAGAGAAGCTCCGCATCCCTAAAGTCTCCCCGTGGCATGCTGGGAATTACTCTTGCTTGGCAGAG
  AACCGTCTGGGTCATGGAAAGATAGACCAGGAAGCTAAGCTGGATGTCCATTATGCTCCCAAGGCGGTGA
  CCACAGTGATTCAGAGCTTCACACCAATCCTGGAAGGAGACAGTGTGACCCTGGTCTGTAGGTACAACTC
  CAGCAATCCAGACGTCACCTCCTACAGATGGAACCCTCAAGGTTCTGGGAGTGTGCTCAAACCCGGAGTG
  CTGAGGATTCAGAAAGTGACATGGGATTCCATGCCTGTCAGCTGTGCTGCCTGCAACCACAAGTGTTCGT
  GGGCCCTCCCTGTCATCCTGAATGTCCACTACGCCCCCAGAGACGTGAAGGTACTGAAGGTAAGCCCCGC
  ATCAGAGATCCGCGCTGGGCAGCGTGTCCTCCTCCAATGCGACTTCGCAGAGAGCAACCCGGCAGAGGTC
  CGCTTCTTCTGGAAGAAGAATGGGAGTCTCGTGCAGGAAGGGAGGTACCTGAGCTTCGGCTCCGTCTCCC
  CAGAAGATTCTGGAAATTATAACTGCATGGTCAACAACTCCATCGGAGAGACCTTGTCACAGGCCTGGAA
  CCTCCAAGTGCTGTATGCTCCTCGGAGGCTGCGTGTGTCCATCAGCCCTGGGGACCATGTGATGGAGGGG
  AAGAAGGCCACCTTGTCCTGTGAGAGTGATGCCAATCCGCCCATCTCACAGTACACCTGGTTTGACTCCA
  GTGGCCAAGACCTCCACTCCTCAGGCCAGAAACTGAGACTGGAACCCCTGGAGGTCCAACACACGGGTTC
  CTACCGCTGCAAAGGGACCAATGGGATAGGCACAGGAGAGTCACCACCCAGCACCCTCACTGTCTACTAC
  AGTCCAGAGACCATCGGCAAGCGGGTCGCCTTGGGACTAGGCTTCTGCCTGACTATCTTCATCCTGGCCA
  TCTGGGGGATGAAAATCCAGAAAAAATGGAAGCAAAACCGCAGCCAGCAGGGGCTTCAGGAAAATTCCAG
  TGGCCAGAGCTTTTTTGTGAGGAACAAAAAGGCTAGGAGGACCCCTCTCTCAGAAGGCCCCCAATCCCAG
  GGATGCTACAATCCGGCAATGGATGACACTGTTAGTTATGCCATCTTGCGCTTTCCAGAGAGTGACACGC
  ACAATGCTGGAGATGCAGGGACCCCAGCAACACAGGCTCCTCCTCCAAACAACAGCGACACGGTCACTTA
  CTCGGTGATACAGAAGCGGCCTATGGGGGATTATGAGAATGTGAATCCGAGCTGCCCGGAGGATGAGAGC
  ATCCATTACTCAGAGCTGGTTCAGTTTGGGGCTGGTAAGCGGCCCCAGGCAAAGGAAGACGTAGACTATG
  TGACCCTCAAGCAC
  
  ACGCGTACGCGGCCGCTCGAGCAGAAACTCATCTCAGAAGAGGATCTGGCAGCAAATGATATCCTGGATT
  ACAAGGATGACGACGATAAGGTTTAA
  Protein Sequence

  >MR231289 representing NM_009845
  Red=Cloning site Green=Tags(s)
  
  MRVHYLWLLLILGHVASARYSSANDWTVDHPQTLFAWEGACIRIPCKYKTPLPKARLDNILLFQNYEFDK
  ATKKFTGTVLYNATKTEKDPESELYLSKQGRVTFLGNRIDNCTLKIHPIRANDSGNLGLRMTAGTERWME
  PIHLNVSEKPFQPYIQMPSEIRESQSVTLTCGLNFSCFGYDILLKWFLEDSEITSITSSVTSITSSVTSS
  IKNVYTESKLTFQPKWTDHGKSVKCQVQHSSKVLSECTVHLDVKYTPKLEIKVNPTEVEKNNSVTMTCRV
  NSSNPKLRTVAVSWFKDGRPLEDQELEQEQQMSKLILHSVTKDMRGKYRCQASNDIGPGESEEVELTVHY
  APEPSRVHIYPSPAEEGQSVELICESLASPSATNYTWYHNRKPIPGDTQEKLRIPKVSPWHAGNYSCLAE
  NRLGHGKIDQEAKLDVHYAPKAVTTVIQSFTPILEGDSVTLVCRYNSSNPDVTSYRWNPQGSGSVLKPGV
  LRIQKVTWDSMPVSCAACNHKCSWALPVILNVHYAPRDVKVLKVSPASEIRAGQRVLLQCDFAESNPAEV
  RFFWKKNGSLVQEGRYLSFGSVSPEDSGNYNCMVNNSIGETLSQAWNLQVLYAPRRLRVSISPGDHVMEG
  KKATLSCESDANPPISQYTWFDSSGQDLHSSGQKLRLEPLEVQHTGSYRCKGTNGIGTGESPPSTLTVYY
  SPETIGKRVALGLGFCLTIFILAIWGMKIQKKWKQNRSQQGLQENSSGQSFFVRNKKARRTPLSEGPQSQ
  GCYNPAMDDTVSYAILRFPESDTHNAGDAGTPATQAPPPNNSDTVTYSVIQKRPMGDYENVNPSCPEDES
  IHYSELVQFGAGKRPQAKEDVDYVTLKH
  
  TRTRPLEQKLISEEDLAANDILDYKDDDDKV
• Restriction Sites: SgfI-MluI Cloning Scheme for this gene Plasmid Map
• ACCN: NM_009845
• ORF Size: 2604 bp
• OTI Disclaimer: The molecular sequence of this clone aligns with the gene accession number as a point of reference only. However, individual transcript sequences of the same gene can differ through naturally occurring variations (e.g. polymorphisms), each with its own valid existence. This clone is substantially in agreement with the reference, but a complete review of all prevailing variants is recommended prior to use. More info
• OTI Annotation: This clone was engineered to express the complete ORF with an expression tag. Expression varies depending on the nature of the gene.

Reference Data

• RefSeq: NM_009845.2, NM_009845.3, NP_033975.3
• RefSeq Size: 3972
• RefSeq ORF: 2607
• Locus ID: 12483
• MW: 97.1 kDa
• Gene Summary: Mediates B-cell B-cell interactions. May be involved in the localization of B-cells in lymphoid tissues. Binds sialylated glycoproteins; one of which is CD45. Preferentially binds to alpha-2,6-linked sialic acid. The sialic acid recognition site can be masked by cis interactions with sialic acids on the same cell surface. Upon ligand induced tyrosine phosphorylation in the immune response seems to be involved in regulation of B-cell antigen receptor signaling. Plays a role in positive regulation through interaction with Src family tyrosine kinases and may also act as an inhibitory receptor by recruiting cytoplasmic phosphatases via their SH2 domains that block signal transduction through dephosphorylation of signaling molecules. [UniProtKB/Swiss-Prot Function]