Dag1 (NM_001276486) Mouse Tagged ORF Clone

CAT#: MR231334

  • TrueORF®

Dag1 (myc-DDK-tagged) - Mouse dystroglycan 1 (Dag1), transcript variant 6


  "NM_001276486" in other vectors (1)


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    • 10 ug

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Specifications

Product Data
Type Mouse Tagged ORF Clone
Tag Myc-DDK
Symbol Dag1
Synonyms D9Wsu13e; DG; Dp71; Dp427
Vector pCMV6-Entry
E. coli Selection Kanamycin (25 ug/mL)
Mammalian Cell Selection Neomycin
Sequence Data
>MR231334 ORF sequence
Red=Cloning site Blue=ORF Green=Tags(s)

TTTTGTAATACGACTCACTATAGGGCGGCCGGGAATTCGTCGACTGGATCCGGTACCGAGGAGATCTGCC
GCCGCGATCGCC

ATGTCTGTGGACAACTGGCTACTGCACCCCCTCTGGGGACAGACCTTTCTCCTCCTCCTGTCTGTGGCTG
TGGCTCAGGCCCACTGGCCCAGTGAACCCTCAGAGGCTGTGAGGGACTGGAAGAACCAGCTTGAGGCGTC
CATGCACTCAGTTCTCTCCGACTTCCAGGAGGCTGTTCCCACCGTGGTTGGCATTCCAGACGGTACGGCT
GTTGTCGGGCGCTCATTTCGAGTGAGCATTCCAACGGATTTAATTGCCTCCAGTGGGGAGATCATCAAGG
TGTCTGCAGCAGGGAAGGAGGCCTTACCGTCTTGGCTACACTGGGACCCACACAGTCATATTTTGGAAGG
CCTTCCTCTTGACACTGATAAAGGTGTGCATTACATCTCAGTGAGTGCTGCACGCCTGGGAGCCAATGGA
AGCCACGTCCCCCAGACTTCCAGTGTGTTCTCTATCGAGGTCTACCCTGAAGACCACAATGAGCCACAGT
CTGTACGGGCAGCCTCATCAGACCCTGGTGAGGTAGTGCCATCTGCCTGTGCTGCTGATGAGCCAGTGAC
TGTCCTTACAGTGATTCTGGATGCTGACCTCACCAAGATGACCCCAAAGCAAAGGATCGATCTGTTGAAC
AGAATGCAGAGCTTCTCAGAAGTAGAACTTCACAACATGAAGTTGGTGCCTGTAGTGAATAATAGACTAT
TTGACATGTCGGCCTTCATGGCTGGCCCAGGAAATGCAAAGAAAGTGGTAGAGAATGGGGCTCTCCTGTC
CTGGAAACTAGGCTGCTCCTTGAACCAGAATAGCGTCCCTGACATCCGTGGTGTAGAAACCCCTGCTAGG
GAGGGTGCTATGTCTGCCCAACTTGGTTATCCTGTGGTGGGTTGGCACATTGCCAATAAGAAGCCCACTC
TCCCCAAACGACTCCGGAGGCAGATCCACGCCACACCTACACCTGTTACTGCCATTGGACCCCCAACCAC
GGCCATTCAGGAGCCACCATCGCGGATAGTGCCTACGCCTACATCTCCAGCCATTGCACCTCCAACAGAG
ACCATGGCTCCTCCTGTCAGGGATCCTGTTCCAGGGAAGCCCACGGTCACCATTCGGACGCGAGGTGCCA
TTATTCAGACCCCAACTCTGGGCCCTATCCAGCCTACTCGGGTGTCAGAAGCTGGTACCACGGTTCCTGG
CCAGATTCGCCCAACACTGACAATTCCTGGCTATGTAGAGCCCACAGCCGTTATTACTCCTCCAACAACT
ACCACAAAGAAGCCACGAGTGTCCACGCCAAAGCCAGCAACGCCTTCAACTGATTCGTCAACTACCACAA
CTCGGAGGCCAACCAAAAAACCACGGACACCCCGACCAGTGCCCCGAGTCACCACCAAAGCACCCATCAC
CAGGTTGGAGACAGCTTCCCCACCCACTCGAATCCGTACTACCACCAGTGGAGTGCCCCGTGGGGGAGAA
CCTAACCAGCGGCCAGAGCTCAAGAATCACATTGACAGGGTAGATGCCTGGGTGGGAACCTATTTTGAGG
TAAAGATTCCATCAGACACCTTCTATGACAATGAGGATACCACTACCGACAAGCTCAAGCTGACCCTGAA
GCTTCGAGAGCAGCAGTTAGTAGGTGAGAAATCGTGGGTTCAGTTTAACAGCAACAGCCAGCTCATGTAT
GGCCTGCCTGACAGCAGCCATGTGGGAAAACATGAGTATTTTATGCATGCCACAGACAAAGGGGGCCTCT
CCGCTGTGGATGCCTTCGAGATCCATGTTCACAAGCGCCCACAAGGGGACAAGGCTCCTGCACGGTTCAA
GGCCAGGCTTGCAGGGGATCCAGCACCGGTGGTGAATGACATTCACAAGAAAATTGCTTTGGTAAAGAAG
CTAGCTTTTGCTTTTGGGGATCGAAACTGCAGCTCCATCACCCTTCAGAACATCACTCGGGGCTCTATCG
TGGTGGAATGGACCAACAACACTCTGCCCCTGGAGCCCTGCCCCAAGGAGCAGATCATAGGGCTGAGCCG
CAGGATTGCTGATGAAAATGGGAAGCCTCGTCCTGCCTTCTCCAATGCTCTGGAGCCTGACTTTAAGGCT
CTGAGTATTGCTGTGACGGGCTCTGGCAGTTGTCGGCACCTCCAGTTTATCCCTGTGGCACCACCCTCTC
CTGGAAGCTCAGCTGCACCAGCCACAGAGGTTCCAGACAGGGACCCCGAGAAGAGCAGTGAGGACGATGT
TTACCTGCACACCGTTATCCCAGCCGTGGTGGTCGCGGCCATCCTGCTCATTGCTGGAATCATTGCTATG
ATCTGCTATCGCAAGAAGAGGAAGGGCAAGCTGACCCTTGAGGACCAGGCCACCTTTATTAAGAAGGGGG
TGCCTATCATCTTTGCGGATGAGCTGGATGACTCTAAGCCCCCGCCCTCTTCCAGCATGCCGCTCATCTT
GCAGGAAGAGAAGGCTCCCCTCCCACCTCCTGAGTACCCCAACCAGAGTATGCCCGAGACCACTCCTCTG
AACCAGGACACTGTGGGAGAGTACACACCCCTGCGGGATGAGGATCCTAACGCACCTCCCTATCAGCCAC
CCCCACCCTTCACGGCTCCCATGGAGGGCAAGGGCTCCCGTCCCAAGAACATGACCCCATACCGATCACC
CCCTCCGTATGTTCCCCCT


ACGCGTACGCGGCCGCTCGAGCAGAAACTCATCTCAGAAGAGGATCTGGCAGCAAATGATATCCTGGATT
ACAAGGATGACGACGATAAG
GTTTAA
>MR231334 protein sequence
Red=Cloning site Green=Tags(s)

MSVDNWLLHPLWGQTFLLLLSVAVAQAHWPSEPSEAVRDWKNQLEASMHSVLSDFQEAVPTVVGIPDGTA
VVGRSFRVSIPTDLIASSGEIIKVSAAGKEALPSWLHWDPHSHILEGLPLDTDKGVHYISVSAARLGANG
SHVPQTSSVFSIEVYPEDHNEPQSVRAASSDPGEVVPSACAADEPVTVLTVILDADLTKMTPKQRIDLLN
RMQSFSEVELHNMKLVPVVNNRLFDMSAFMAGPGNAKKVVENGALLSWKLGCSLNQNSVPDIRGVETPAR
EGAMSAQLGYPVVGWHIANKKPTLPKRLRRQIHATPTPVTAIGPPTTAIQEPPSRIVPTPTSPAIAPPTE
TMAPPVRDPVPGKPTVTIRTRGAIIQTPTLGPIQPTRVSEAGTTVPGQIRPTLTIPGYVEPTAVITPPTT
TTKKPRVSTPKPATPSTDSSTTTTRRPTKKPRTPRPVPRVTTKAPITRLETASPPTRIRTTTSGVPRGGE
PNQRPELKNHIDRVDAWVGTYFEVKIPSDTFYDNEDTTTDKLKLTLKLREQQLVGEKSWVQFNSNSQLMY
GLPDSSHVGKHEYFMHATDKGGLSAVDAFEIHVHKRPQGDKAPARFKARLAGDPAPVVNDIHKKIALVKK
LAFAFGDRNCSSITLQNITRGSIVVEWTNNTLPLEPCPKEQIIGLSRRIADENGKPRPAFSNALEPDFKA
LSIAVTGSGSCRHLQFIPVAPPSPGSSAAPATEVPDRDPEKSSEDDVYLHTVIPAVVVAAILLIAGIIAM
ICYRKKRKGKLTLEDQATFIKKGVPIIFADELDDSKPPPSSSMPLILQEEKAPLPPPEYPNQSMPETTPL
NQDTVGEYTPLRDEDPNAPPYQPPPPFTAPMEGKGSRPKNMTPYRSPPPYVPP

TRTRPLEQKLISEEDLAANDILDYKDDDDKV
Restriction Sites SgfI-MluI      Cloning Scheme for this gene      Plasmid Map     
ACCN NM_001276486
ORF Size 2679 bp
OTI Disclaimer The molecular sequence of this clone aligns with the gene accession number as a point of reference only. However, individual transcript sequences of the same gene can differ through naturally occurring variations (e.g. polymorphisms), each with its own valid existence. This clone is substantially in agreement with the reference, but a complete review of all prevailing variants is recommended prior to use. More info
OTI Annotation This clone was engineered to express the complete ORF with an expression tag. Expression varies depending on the nature of the gene.
Reference Data
RefSeq NM_001276486.1, NP_001263415.1
RefSeq Size 5435
RefSeq ORF 2682
Locus ID 13138
MW 96.9 kDa
Gene Summary This gene encodes dystroglycan, a central component of dystrophin-glycoprotein complex that links the extracellular matrix and the cytoskeleton in the skeletal muscle. The encoded preproprotein undergoes O- and N-glycosylation, and proteolytic processing to generate alpha and beta subunits. A complete lack of the encoded protein in mice results in embryonic lethality due to the disorganization of Reichert's membrane. Chimeric mice deficient in the encoded protein overcome embryonic lethality but develop a progressive muscular dystrophy. Alternative splicing results in multiple transcript variants, all encoding the same protein. [provided by RefSeq, Nov 2015]

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