Ap1b1 (NM_001243043) Mouse Tagged ORF Clone

CAT#: MR231424

Ap1b1 (myc-DDK-tagged) - Mouse adaptor protein complex AP-1, beta 1 subunit (Ap1b1), transcript variant 1

Specifications

Product Data

• Type: Mouse Tagged ORF Clone
• Tag: Myc-DDK
• Symbol: Ap1b1
• Synonyms: Adtb1; b2b1660Clo
• Vector: pCMV6-Entry
• E. coli Selection: Kanamycin (25 ug/mL)
• Mammalian Cell Selection: Neomycin
• Sequence Data:
  ORF Nucleotide Sequence

  >MR231424 representing NM_001243043
  Red=Cloning site Blue=ORF Green=Tags(s)
  
  TTTTGTAATACGACTCACTATAGGGCGGCCGGGAATTCGTCGACTGGATCCGGTACCGAGGAGATCTGCC
  GCCGCGATCGCC
  
  ATGACTGACTCAAAATACTTCACCACAACCAAGAAAGGGGAGATCTTTGAGCTGAAGGCAGAGCTTAACA
  GTGACAAGAAGGAGAAGAAGAAGGAGGCAGTGAAGAAAGTGATTGCATCAATGACTGTGGGCAAAGATGT
  CAGTGCTCTCTTCCCTGATGTGGTGAACTGCATGCAGACAGACAACCTGGAGCTAAAGAAGCTGGTGTAC
  CTGTACTTGATGAACTATGCCAAGAGTCAGCCTGACATGGCCATTATGGCTGTCAACACCTTTGTGAAGG
  ACTGTGAGGACCCCAATCCTCTCATCCGGGCCCTGGCTGTGAGGACTATGGGCTGCATCCGAGTTGACAA
  GATTACAGAGTACTTGTGTGAACCACTCCGGAAGTGCCTGAAGGATGAAGACCCATATGTGCGCAAGACA
  GCGGCTGTGTGTGTGGCCAAGCTCCATGACATCAATGCCCAGCTGGTAGAAGACCAGGGCTTCCTGGACA
  CGCTTAAAGACCTCATCTCAGACTCTAACCCCATGGTGGTAGCGAATGCAGTGGCTGCCCTGTCAGAGAT
  CGCTGAGTCTCACCCCAGTAGCAACCTGCTTGACTTGAACCCACAGTCTATCAACAAGCTGCTGACAGCC
  CTTAATGAGTGCACTGAATGGGGCCAGATCTTCATTCTGGACTGTCTGGCCAACTACATGCCCAAGGATG
  ACCGTGAGGCCCAGAGCATCTGTGAGCGGGTCACTCCTAGACTCTCCCATGCCAACTCTGCTGTGGTGCT
  GTCTGCGGTGAAGGTGCTGATGAAGTTCATGGAGATGCTGTCCAAGGACCTGGACTACTATGCCACACTG
  CTCAAGAAGCTGGCCCCTCCCCTGGTCACACTGCTGTCCGCAGAGCCAGAGCTGCAGTATGTGGCCCTGC
  GCAACATCAATCTCATTGTACAGAAAAGGCCAGAGATCTTGAAGCATGAAATGAAGGTTTTCTTTGTGAA
  GTACAATGACCCCATCTACGTGAAGCTGGAGAAACTGGACATTATGATCCGCCTGGCCTCCCAGGCCAAC
  ATTGCCCAGGTATTGGCAGAACTCAAAGAGTATGCTACAGAAGTAGACGTGGATTTCGTACGGAAGGCAG
  TGCGTGCCATTGGCCGCTGTGCCATCAAGGTGGAGCAATCTGCAGAGCGCTGTGTGAGCACATTGCTTGA
  TCTCATCCAGACCAAGGTCAACTATGTGGTTCAGGAAGCCATTGTGGTCATTAAGGACATCTTCCGCAAG
  TACCCCAACAAGTATGAGAGTGTGATAGCTACACTGTGTGAGAATCTGGACTCTCTGGATGAGCCTGAGG
  CCCGAGCCGCTATGATCTGGATAGTGGGCGAGTATGCCGAGCGGATTGACAATGCAGACGAGCTGCTCGA
  GAGCTTCCTGGAAGGTTTCCATGATGAGAGCACTCAGGTCCAGCTACAGCTACTGACAGCCATTGTGAAA
  CTCTTCCTGAAGAAGCCAACAGAGACTCAGGAGCTGGTGCAGCAGGTCCTCAGTTTGGCCACTCAGGACT
  CAGACAATCCAGACCTTCGGGACCGTGGCTACATCTACTGGCGCTTGCTCTCCACGGACCCTGTAGCTGC
  TAAAGAGGTGGTACTGGCTGAGAAGCCCCTCATCTCAGAGGAGACAGACCTTATTGAGCCTACACTTCTG
  GATGAACTCATCTGCTACATTGGCACGCTGGCCTCTGTCTACCACAAGCCTCCCAACGCCTTTGTGGAGG
  GTGGCCGGGGAGTGGTACATAAGAGCCTGCCACCCCGCACTGCCTCGAGTGAGAGCACAGAGAGCCCTGA
  GACAGCTCCTGCTGGAGCACCTGCTGGTGATCAGCCTGATGTCATTCCTGCCCAGGGTGACCTGTTAGGT
  GACCTCCTCAACCTGGACCTTGGGCCCCCAGTGAGTGGCCCACCCTTAGCTGCCTCCTCCGTGCAAATGG
  GAGCTGTGGATCTTCTTGGTGGTGGCCTCGACAGCCTGATGGGGGATGAGTCTGAAGGGATTGGAGATAG
  CAACTTTGGGGCACCTTCAGCATCAGTGGCCGCAGCACCAGCACCAGCCAGACTTGGAGCACCCATCAGC
  AGTGGCTTAAGTGACCTCTTTGACCTGACCAGTGGTGTGGGCACCCTTTCAGGATCCTATGTAGCTCCCA
  AAGCAGTCTGGCTCCCTGCCATGAAGGCCAAGGGACTGGAGATCTCTGGCACCTTCACCCGGCAGGCTGG
  CTCCATCTCTATGGACCTGCAGCTGACTAACAAGGCCCTGCAGGTCATGACAGATTTTGCTATCCAGTTC
  AATCGTAACAGCTTTGGCTTGGCCCCTGCTGCACCTCTCCAGGTGCACGTACCACTCAGCCCCAACCAGA
  CCGTGGAGATCTCCCTGCCTCTTAACACAGTGGGCTCAGTCTTGAAGATGGAGCCTCTAAACAATCTTCA
  GGTGGCTGTGAAGAACAACATTGACGTCTTCTACTTCAGCACTTTGTACCCACTGCATGTCCTCTTCGTG
  GAGGATGGGAAGATGGACCGGCAGATGTTCCTGGCCACATGGAAGGACATTGCCAACGAGAACGAAGCCC
  AGTTCCAGATCAGAGACTGCCCGCTTAACACAGAGGCTGCGAGCAACAAGCTACAGAGCAGTAACATTTT
  CACTGTCGCCAAGAGGAACGTGGAGGGCCAGGATATGCTCTACCAATCTCTGAAGTTGACCAATGGCATC
  TGGGTGCTGGCGGAGCTGCGGATCCAGCCAGGCAACCCCAGCTTCACGGACTTGGAGCTGTCCCTGAAGT
  GTCGAGCACCGGAGGTGTCCCAGCATGTATACCAGGCCTACGAGACTATCCTCAAGAAC
  
  ACGCGTACGCGGCCGCTCGAGCAGAAACTCATCTCAGAAGAGGATCTGGCAGCAAATGATATCCTGGATT
  ACAAGGATGACGACGATAAGGTTTAA
  Protein Sequence

  >MR231424 representing NM_001243043
  Red=Cloning site Green=Tags(s)
  
  MTDSKYFTTTKKGEIFELKAELNSDKKEKKKEAVKKVIASMTVGKDVSALFPDVVNCMQTDNLELKKLVY
  LYLMNYAKSQPDMAIMAVNTFVKDCEDPNPLIRALAVRTMGCIRVDKITEYLCEPLRKCLKDEDPYVRKT
  AAVCVAKLHDINAQLVEDQGFLDTLKDLISDSNPMVVANAVAALSEIAESHPSSNLLDLNPQSINKLLTA
  LNECTEWGQIFILDCLANYMPKDDREAQSICERVTPRLSHANSAVVLSAVKVLMKFMEMLSKDLDYYATL
  LKKLAPPLVTLLSAEPELQYVALRNINLIVQKRPEILKHEMKVFFVKYNDPIYVKLEKLDIMIRLASQAN
  IAQVLAELKEYATEVDVDFVRKAVRAIGRCAIKVEQSAERCVSTLLDLIQTKVNYVVQEAIVVIKDIFRK
  YPNKYESVIATLCENLDSLDEPEARAAMIWIVGEYAERIDNADELLESFLEGFHDESTQVQLQLLTAIVK
  LFLKKPTETQELVQQVLSLATQDSDNPDLRDRGYIYWRLLSTDPVAAKEVVLAEKPLISEETDLIEPTLL
  DELICYIGTLASVYHKPPNAFVEGGRGVVHKSLPPRTASSESTESPETAPAGAPAGDQPDVIPAQGDLLG
  DLLNLDLGPPVSGPPLAASSVQMGAVDLLGGGLDSLMGDESEGIGDSNFGAPSASVAAAPAPARLGAPIS
  SGLSDLFDLTSGVGTLSGSYVAPKAVWLPAMKAKGLEISGTFTRQAGSISMDLQLTNKALQVMTDFAIQF
  NRNSFGLAPAAPLQVHVPLSPNQTVEISLPLNTVGSVLKMEPLNNLQVAVKNNIDVFYFSTLYPLHVLFV
  EDGKMDRQMFLATWKDIANENEAQFQIRDCPLNTEAASNKLQSSNIFTVAKRNVEGQDMLYQSLKLTNGI
  WVLAELRIQPGNPSFTDLELSLKCRAPEVSQHVYQAYETILKN
  
  TRTRPLEQKLISEEDLAANDILDYKDDDDKV
• Restriction Sites: SgfI-MluI Cloning Scheme for this gene Plasmid Map
• ACCN: NM_001243043
• ORF Size: 2859 bp
• OTI Disclaimer: The molecular sequence of this clone aligns with the gene accession number as a point of reference only. However, individual transcript sequences of the same gene can differ through naturally occurring variations (e.g. polymorphisms), each with its own valid existence. This clone is substantially in agreement with the reference, but a complete review of all prevailing variants is recommended prior to use. More info
• OTI Annotation: This clone was engineered to express the complete ORF with an expression tag. Expression varies depending on the nature of the gene.

Reference Data

• RefSeq: NM_001243043.1, NP_001229972.1
• RefSeq Size: 4336
• RefSeq ORF: 2862
• Locus ID: 11764
• MW: 105.4 kDa
• Gene Summary: Subunit of clathrin-associated adaptor protein complex 1 that plays a role in protein sorting in the late-Golgi/trans-Golgi network (TGN) and/or endosomes. The AP complexes mediate both the recruitment of clathrin to membranes and the recognition of sorting signals within the cytosolic tails of transmembrane cargo molecules. [UniProtKB/Swiss-Prot Function]