Ano1 (NM_001242349) Mouse Tagged ORF Clone

CAT#: MR231486

Ano1 (myc-DDK-tagged) - Mouse anoctamin 1, calcium activated chloride channel (Ano1), transcript variant 2

Specifications

Product Data

• Type: Mouse Tagged ORF Clone
• Tag: Myc-DDK
• Symbol: Ano1
• Synonyms: Tmem16a
• Vector: pCMV6-Entry
• E. coli Selection: Kanamycin (25 ug/mL)
• Mammalian Cell Selection: Neomycin
• Sequence Data:
  ORF Nucleotide Sequence

  >MR231486 ORF sequence
  Red=Cloning site Blue=ORF Green=Tags(s)
  
  TTTTGTAATACGACTCACTATAGGGCGGCCGGGAATTCGTCGACTGGATCCGGTACCGAGGAGATCTGCC
  GCCGCGATCGCC
  
  ATGCAGGACGCGCAGGACAGCGACATCGGCCTGGAGGGCTTGACCCCAGAGGGAACCTCTGCGGACCGAG
  AGTGCCAGCGGGGACCGGAAACAATCGCGCACGAGGCACAGGACGCAGGAACCCCCAACTCAGGTGACGC
  CACCGGGGTTGTGGATGGGGAGCGCGAGGCCACCATGAGGGTCCCCGAGAAGTACTCGACGCTCCCGGCG
  GAGGACCGCAGCGTCCACATCGTGAACATCTGCGCCATCGAGGACCTGGGCTACCTGCCGTCCGAGGGCA
  CGTTGCTGAACTCTCTGTCCGTGGACCCCGACGCCGAATGCAAGTATGGACTATACTTCAGGGATGGCAA
  ACGGAAGGTGGACTACATCTTGGTATACCATCACAAGAGAGCCTCAGGGAGCAGGACTCTGGCCAGGAGG
  GGACTACAAAATGACATGGTCCTGGGGACCCGCAGCGTCAGGCAGGACCAGCCCCTTCCCGGGAAGGGGA
  GCCCTGTGGATGCAGGCTCACCGGAAGTCCCCATGGATTACCATGAAGATGACAAACGCTTCAGACGGGA
  GGAATATGAGGGCAACCTGTTGGAGGCAGGCCTGGAGTTGGAGAATGACGAGGATACCAAAATCCATGGT
  GTCGGGTTTGTGAAGATCCATGCGCCCTGGCATGTGCTCTGTAGGGAAGCTGAGTTTTTGAAACTAAAGA
  TGCCCACAAAGAAGGTGTACCACATCAGTGAGACGCGAGGCCTCCTGAAAACCATCAACTCGGTTCTGCA
  GAAGATCACAGACCCCATCCAGCCCAAGGTGGCTGAGCACAGGCCACAGACCACAAAGAGGCTCTCCTAT
  CCCTTCTCCCGGGAGAAGCAACACCTATTCGACCTGACTGACAGGGACTCTTTTTTCGACAGCAAAACCC
  GGAGCACAATAGTCTATGAAATCCTGAAGAGAACAACGTGCACCAAGGCCAAGTACAGCATGGGTATCAC
  CAGCCTCCTGGCCAATGGCGTATACTCAGCTGCATACCCTCTGCACGATGGGGACTATGAGGGTGACAAC
  GTTGAGTTCAACGACAGGAAACTCCTGTATGAGGAATGGGCAAGTTACGGAGTCTTCTACAAATACCAGC
  CCATTGACCTGGTCAGGAAATACTTTGGTGAGAAGGTTGGCCTGTACTTTGCCTGGCTTGGAGCCTACAC
  CCAGATGCTCATCCCTGCCTCGATCGTGGGTGTCATTGTCTTTCTCTATGGATGTGCCACTGTGGACGAA
  AACATCCCCAGTATGGAGATGTGTGACCAGAGATACAACATCACCATGTGTCCTCTGTGTGACAAGACCT
  GCAGCTACTGGAAGATGAGCTCAGCCTGTGCCACAGCCCGTGCCAGTCACCTTTTTGATAACCCTGCCAC
  CGTCTTCTTCTCTGTGTTTATGGCCCTCTGGGCTGCCACTTTCATGGAGCACTGGAAACGGAAGCAGATG
  AGGCTCAACTACCGATGGGACCTCACAGGCTTCGAGGAGGAGGAGGATCATCCCAGAGCAGAGTATGAAG
  CCAGAGTCTTAGAGAAGTCACTGAGAAAAGAATCCAGAAACAAAGAGACCGACAAGGTGAAGCTGACCTG
  GAGGGACCGATTCCCAGCCTATTTCACCAATCTTGTCTCCATCATCTTCATGATCGCAGTGACATTTGCA
  ATCGTCCTCGGAGTTATCATCTATAGAATCTCCACAGCTGCAGCCTTGGCCATGAACTCCTCCCCGTCTG
  TGCGGTCCAACATCCGGGTTACAGTCACGGCCACCGCTGTTATCATCAACCTCGTGGTCATCATTCTGCT
  GGATGAAGTTTACGGCTGCATTGCCAGGTGGCTCACCAAGATTGAGGTCCCAAAGACAGAGAAGAGCTTT
  GAGGAGAGGCTAACCTTCAAGGCCTTCCTGCTCAAGTTTGTGAACTCTTACACTCCCATCTTCTATGTCG
  CCTTCTTCAAAGGCCGGTTTGTTGGTCGGCCCGGTGACTACGTGTACATCTTCCGCTCTTTCCGGATGGA
  GGAGTGTGCCCCGGGCGGCTGCCTCATGGAGCTCTGTATCCAGCTGAGCATCATTATGCTGGGCAAGCAG
  CTAATCCAGAACAATCTCTTCGAGATTGGCATCCCGAAGATGAAAAAGTTCATCCGCTACCTGAAGCTGC
  GCAGACAGAGCCCCTCAGACCGTGAAGAGTACGTGAAGCGGAAGCAGCGCTATGAGGTGGACTTCAACCT
  CGAACCTTTCGCCGGCCTCACGCCCGAGTACATGGAAATGATCATTCAGTTCGGCTTTGTCACCCTGTTT
  GTTGCGTCCTTCCCTCTGGCTCCACTCTTCGCCCTGCTAAACAACATCATTGAGATCCGCCTGGATGCCA
  AAAAGTTTGTCACCGAGCTACGGAGGCCAGTAGCCATCAGAGCCAAAGACATCGGCATCTGGTATAACAT
  CCTCAGAGGTGTTGGGAAGCTGGCTGTCATCATTAATGCCTTTGTGATCTCCTTCACGTCTGACTTCATC
  CCTCGCCTGGTGTACCTCTACATGTACAGTCAGAATGGGACCATGCACGGCTTCGTCAATCACACGCTCT
  CTTCCTTCAATGTCAGCGACTTCCAGAATGGCACAGCACCCAATGACCCACTGGACCTGGGCTATGAGGT
  TCAGATCTGCAGGTATAAAGATTACCGGGAACCCCCATGGTCAGAACACAAGTATGACATCTCCAAAGAC
  TTCTGGGCTGTCCTGGCCGCCCGACTGGCATTTGTCATTGTCTTCCAGAACCTGGTGATGTTCATGAGTG
  ACTTTGTGGACTGGGTGATCCCTGATATCCCCAAAGACATCAGCCAGCAGATCCACAAAGAGAAGGTTCT
  CATGGTGGAGCTGTTTATGCGTGAGGAGCAGGGCAAGCAGCAGCTACTGGACACATGGATGGAGAAGGAG
  AAGCCAAGGGATGTGCCTTGTAACAACCACAGCCCCACAACCCACCCAGAGGCAGGCGACGGCAGCCCAG
  TCCCCAGCTACGAGTACCATGGGGACGCGCTG
  
  ACGCGTACGCGGCCGCTCGAGCAGAAACTCATCTCAGAAGAGGATCTGGCAGCAAATGATATCCTGGATT
  ACAAGGATGACGACGATAAGGTTTAA
  Protein Sequence

  >MR231486 protein sequence
  Red=Cloning site Green=Tags(s)
  
  MQDAQDSDIGLEGLTPEGTSADRECQRGPETIAHEAQDAGTPNSGDATGVVDGEREATMRVPEKYSTLPA
  EDRSVHIVNICAIEDLGYLPSEGTLLNSLSVDPDAECKYGLYFRDGKRKVDYILVYHHKRASGSRTLARR
  GLQNDMVLGTRSVRQDQPLPGKGSPVDAGSPEVPMDYHEDDKRFRREEYEGNLLEAGLELENDEDTKIHG
  VGFVKIHAPWHVLCREAEFLKLKMPTKKVYHISETRGLLKTINSVLQKITDPIQPKVAEHRPQTTKRLSY
  PFSREKQHLFDLTDRDSFFDSKTRSTIVYEILKRTTCTKAKYSMGITSLLANGVYSAAYPLHDGDYEGDN
  VEFNDRKLLYEEWASYGVFYKYQPIDLVRKYFGEKVGLYFAWLGAYTQMLIPASIVGVIVFLYGCATVDE
  NIPSMEMCDQRYNITMCPLCDKTCSYWKMSSACATARASHLFDNPATVFFSVFMALWAATFMEHWKRKQM
  RLNYRWDLTGFEEEEDHPRAEYEARVLEKSLRKESRNKETDKVKLTWRDRFPAYFTNLVSIIFMIAVTFA
  IVLGVIIYRISTAAALAMNSSPSVRSNIRVTVTATAVIINLVVIILLDEVYGCIARWLTKIEVPKTEKSF
  EERLTFKAFLLKFVNSYTPIFYVAFFKGRFVGRPGDYVYIFRSFRMEECAPGGCLMELCIQLSIIMLGKQ
  LIQNNLFEIGIPKMKKFIRYLKLRRQSPSDREEYVKRKQRYEVDFNLEPFAGLTPEYMEMIIQFGFVTLF
  VASFPLAPLFALLNNIIEIRLDAKKFVTELRRPVAIRAKDIGIWYNILRGVGKLAVIINAFVISFTSDFI
  PRLVYLYMYSQNGTMHGFVNHTLSSFNVSDFQNGTAPNDPLDLGYEVQICRYKDYREPPWSEHKYDISKD
  FWAVLAARLAFVIVFQNLVMFMSDFVDWVIPDIPKDISQQIHKEKVLMVELFMREEQGKQQLLDTWMEKE
  KPRDVPCNNHSPTTHPEAGDGSPVPSYEYHGDAL
  
  TRTRPLEQKLISEEDLAANDILDYKDDDDKV
• Restriction Sites: SgfI-MluI Cloning Scheme for this gene Plasmid Map
• ACCN: NM_001242349
• ORF Size: 3042 bp
• OTI Disclaimer: The molecular sequence of this clone aligns with the gene accession number as a point of reference only. However, individual transcript sequences of the same gene can differ through naturally occurring variations (e.g. polymorphisms), each with its own valid existence. This clone is substantially in agreement with the reference, but a complete review of all prevailing variants is recommended prior to use. More info
• OTI Annotation: This clone was engineered to express the complete ORF with an expression tag. Expression varies depending on the nature of the gene.

Reference Data

• RefSeq: NM_001242349.1, NP_001229278.1
• RefSeq Size: 4605
• RefSeq ORF: 3045
• Locus ID: 101772
• MW: 116.4 kDa