Dgkh (NM_001281794) Mouse Tagged ORF Clone

CAT#: MR231503

Dgkh (myc-DDK-tagged) - Mouse diacylglycerol kinase, eta (Dgkh), transcript variant 3

Specifications

Product Data

• Type: Mouse Tagged ORF Clone
• Tag: Myc-DDK
• Symbol: Dgkh
• Synonyms: 5930402B05Rik; D130015C16; DGK
• Vector: pCMV6-Entry
• E. coli Selection: Kanamycin (25 ug/mL)
• Mammalian Cell Selection: Neomycin
• Sequence Data:
  ORF Nucleotide Sequence

  >MR231503 representing NM_001281794
  Red=Cloning site Blue=ORF Green=Tags(s)
  
  TTTTGTAATACGACTCACTATAGGGCGGCCGGGAATTCGTCGACTGGATCCGGTACCGAGGAGATCTGCC
  GCCGCGATCGCC
  
  ATGCTATGTGCTGAGAACAGGAAAGAGATGGAGGTCTGGATCAGCTCTCTGAAGTCAGTGCAGAGCAGAG
  AACCCTATGAGGTGGCCCAGTTCAATGTGGAACATTTCTCAGGGATGCACAACTGGTACGCCTGCTCCCA
  CGCCCGGCCCACCTTCTGTAACGTGTGCAGAGAGAGTCTCTCTGGAGTGACCTCCCACGGCCTGTCCTGT
  GAAGTGTGTAAGTTCAAGGCTCATAAAAGATGTGCAGTGAGGGCAACAAACAACTGCAAATGGACCACGC
  TGGCCTCCATCGGGAAGGACATCATAGAGGACGAGGATGGTGTTGCCATGCCTCACCAGTGGCTCGAAGG
  CAACTTGCCTGTGAGTGCCAAGTGTGCAGTCTGTGACAAAACCTGTGGCAGTGTGCTTCGCCTGCAAGAT
  TGGAAATGCCTTTGGTGTAAGACAATGGTGCACACTGCCTGCAAAGATGTGTACCATCCCGTCTGTCCCC
  TTGGCCAGTGTAAAGTCTCCATCATACCTCCGATTGCACTAAATAGCACAGACTCTGATGGTTTCTGTCG
  AGCAACCTTTTCGTTCTGTGTCAGCCCCCTCTTGGTGTTCGTCAACTCTAAGAGTGGAGATAATCAGGGA
  GTGAAGTTCCTTCGTCGCTTTAAACAGTTGCTCAACCCAGCTCAGGTGTTTGATTTAATGAACGGAGGGC
  CTTATTTAGGCTTGAGATTGTTTCAAAAGTTTGACAACTTTCGGATCCTTGTCTGTGGAGGAGATGGAAG
  TGTAGGCTGGGTTTTGTCAGAGATTGATAAACTGAACTTGCATAAACAGTGCCAGCTTGGGGTGCTACCC
  TTGGGTACCGGCAATGACCTTGCTCGAGTTCTTGGCTGGGGAGGTTCATATGATGATGACACCCAACTCC
  CTCAGATACTAGAGAAGCTGGAACGAGCCAGTACCAAAATGCTGGACAGGTGGAGTATAATGACCTATGA
  GCTCAAATTGCCAGCAAAGTCGTCTCTACTTCCTGAACCTGTGGCAGCAACTGAAGAATTTTACATGACA
  ATTTATGAAGACTCCGTTGCAAACCATCTTACGAAAATCGTCAACTCTGATGAGCATGCGGTGGTCATAT
  CGTCTGCCAAGATACTCTGTGAAACCGTGAAGGACTTCGTTGCCAAAGTGGAGAAGGCACAGGACAGAAC
  ACTGGAAAATACAGTAGTAGCCGAAGCCGTGGCCAGTAAATGCTCAGTCCTAAACGAGAAGCTTGAGCAG
  TTGCTACAAGCCTTGCACGCGGACTCTCAGGCCAGTCGAGTCCCCCCAGGAATCGGCCCTGCCATCCCCG
  AAGAGGACACCGTGGAGTCCGCCAGTGACGAGTCCTTGGGTGAAAGCAAGGACCAGCTGGTGAATGACAT
  TGGGAAACCCTCCTCCCAGAAAGCCGTGAAGCCCCGGGAGATAATGCTGCGGGCCAACAGTCTAAAGAAA
  GCGGTGAGGCAAGTCATTGAAGAAGCTGAAAAAGTTATGGATGAGCCAGCAGTGCAGCCCTCTGAGCCAG
  TCAGTCCGTCTTGTGACTATGATACAGAAACAGATGAAGCTAAAGAGGACGACGCGAAAGAGTCGCTATC
  AGCCAAAACCACATCTCAGTCTCCTGATGCCCAGGCAAGCTGTGGCCATCCCCAAACTGACTCTGTCGCT
  GGTCCAGCCATGGCCACCACCAAAGAAAACCTCCCTGTGCTCAACACTAGAATCATCTGCCCAGGTTTGA
  GAGCAGGACTTGCTGCCTCCATTGCTGGGAGTTCTATCATCAACAAAATGTTGCTGGCGAACATCGATCC
  TTTCGGGGCAACACCGTTTATTGACCCAGATCTAGACTCACTAGATGGATACTCTGAAAAATGTGTCATG
  AACAACTACTTTGGGATTGGATTAGATGCAAAAATTTCATTAGAATTTAATAATAAGAGAGAGGAGCACC
  CTGAAAAATGCAGAAGTCGAACTAAAAACTTGATGTGGTACGGAGTCCTTGGGACCCGGGAGCTACTACA
  GAGATCGTACAAGAATCTGGAGCAGAGGGTTCAACTTGAGTGTGATGGGCAGTATATACCTCTTCCTAGT
  CTACAAGGCATAGCGGTGTTGAACATCCCTAGCTACGCCGGAGGCACCAACTTCTGGGGCGGAACCAAAG
  AGGATGATATATTTGCTGCGCCATCCTTTGATGACAAGATTCTAGAAGTTGTCGCAGTGTTTGACAGTGT
  GCAGATGGCAGTATCAAGGGTCATTAAATTGCAGCATCACCGGATAGCACAGTGCCGTACGGTAAAAATC
  ACGATATTTGGCGACGAGGGAGTCCCAGTGCAGGTGGATGGTGAAGCATGGGTCCAGCCTCCAGGGATCA
  TCAAGATTGTGCACAAAAACAGAGCTCAGATGCTAACCAGAGACAGAGCCTTTGAAAGCACCCTGAAGTC
  TTGGGAAGACAAGCAGAAGTGTGACTCTGGGAAGCCAGTTCTTCGAACCAACCTGTACATCCATCCCGCC
  CCTGACCTGGCAACAGAGGAAGTGTCACAGATGCGGCTCTGCTCCCAGGCTGCGGAGGAACTCATCACTA
  GGATTTGTGACGCAGCCACGATTCACTGTCTCCTGGAGCAGGAGCTGGCACATGCAGTGAATGCCTGCTC
  ACACGCCCTGAACAAAGCCAACCCACGCTTCCCGGAGAGCCTTACGAGAGACACTGCCACCGAGATAGCC
  ATCAACGTCAAGGCGCTCTATAATGAGACAGAGGCTTTGCTAGTTGGCAGGGTCCCTTTGCACTTGGAAT
  CTCCTCACGAAGAGCGGGTGTCCAGTGCCTTACACTCTGTGGAGATGGAGCTTCAGAAGTTAACGGAGAT
  TCCCTGGCTTTATTACATCCTCCGCCCCAGTGAGGACGAGGAGCCTCCTCTGGATTGTACCAAAAGAAAC
  AACAAAAGCACAGTCTTTCGCATAGTGCCAAAGTTTAAAAAGGAAAAGGCTCAGAAACAGAAAACAAGTT
  CGCAGCCTGAATTGGGGCACAGAGGAAGTTGCTGCTTGGCTGGATCTGCTCAATTTGGGAGAGTACAAAG
  AAATCTTCATCCGTCA
  
  ACGCGTACGCGGCCGCTCGAGCAGAAACTCATCTCAGAAGAGGATCTGGCAGCAAATGATATCCTGGATT
  ACAAGGATGACGACGATAAGGTTTAA
  Protein Sequence

  >MR231503 representing NM_001281794
  Red=Cloning site Green=Tags(s)
  
  MLCAENRKEMEVWISSLKSVQSREPYEVAQFNVEHFSGMHNWYACSHARPTFCNVCRESLSGVTSHGLSC
  EVCKFKAHKRCAVRATNNCKWTTLASIGKDIIEDEDGVAMPHQWLEGNLPVSAKCAVCDKTCGSVLRLQD
  WKCLWCKTMVHTACKDVYHPVCPLGQCKVSIIPPIALNSTDSDGFCRATFSFCVSPLLVFVNSKSGDNQG
  VKFLRRFKQLLNPAQVFDLMNGGPYLGLRLFQKFDNFRILVCGGDGSVGWVLSEIDKLNLHKQCQLGVLP
  LGTGNDLARVLGWGGSYDDDTQLPQILEKLERASTKMLDRWSIMTYELKLPAKSSLLPEPVAATEEFYMT
  IYEDSVANHLTKIVNSDEHAVVISSAKILCETVKDFVAKVEKAQDRTLENTVVAEAVASKCSVLNEKLEQ
  LLQALHADSQASRVPPGIGPAIPEEDTVESASDESLGESKDQLVNDIGKPSSQKAVKPREIMLRANSLKK
  AVRQVIEEAEKVMDEPAVQPSEPVSPSCDYDTETDEAKEDDAKESLSAKTTSQSPDAQASCGHPQTDSVA
  GPAMATTKENLPVLNTRIICPGLRAGLAASIAGSSIINKMLLANIDPFGATPFIDPDLDSLDGYSEKCVM
  NNYFGIGLDAKISLEFNNKREEHPEKCRSRTKNLMWYGVLGTRELLQRSYKNLEQRVQLECDGQYIPLPS
  LQGIAVLNIPSYAGGTNFWGGTKEDDIFAAPSFDDKILEVVAVFDSVQMAVSRVIKLQHHRIAQCRTVKI
  TIFGDEGVPVQVDGEAWVQPPGIIKIVHKNRAQMLTRDRAFESTLKSWEDKQKCDSGKPVLRTNLYIHPA
  PDLATEEVSQMRLCSQAAEELITRICDAATIHCLLEQELAHAVNACSHALNKANPRFPESLTRDTATEIA
  INVKALYNETEALLVGRVPLHLESPHEERVSSALHSVEMELQKLTEIPWLYYILRPSEDEEPPLDCTKRN
  NKSTVFRIVPKFKKEKAQKQKTSSQPELGHRGSCCLAGSAQFGRVQRNLHPS
  
  TRTRPLEQKLISEEDLAANDILDYKDDDDKV
• Restriction Sites: SgfI-MluI Cloning Scheme for this gene Plasmid Map
• ACCN: NM_001281794
• ORF Size: 3096 bp
• OTI Disclaimer: The molecular sequence of this clone aligns with the gene accession number as a point of reference only. However, individual transcript sequences of the same gene can differ through naturally occurring variations (e.g. polymorphisms), each with its own valid existence. This clone is substantially in agreement with the reference, but a complete review of all prevailing variants is recommended prior to use. More info
• OTI Annotation: This clone was engineered to express the complete ORF with an expression tag. Expression varies depending on the nature of the gene.

Reference Data

• RefSeq: NM_001281794.1, NP_001268723.1
• RefSeq Size: 3781
• RefSeq ORF: 3099
• Locus ID: 380921
• MW: 114.7 kDa