Pdgfra (NM_011058) Mouse Tagged ORF Clone

CAT#: MR231568

Pdgfra (myc-DDK-tagged) - Mouse platelet derived growth factor receptor, alpha polypeptide (Pdgfra), transcript variant 1

Specifications

Product Data

• Type: Mouse Tagged ORF Clone
• Tag: Myc-DDK
• Symbol: Pdgfra
• Synonyms: AI115593; CD140a; Pdgfr-2
• Vector: pCMV6-Entry
• E. coli Selection: Kanamycin (25 ug/mL)
• Mammalian Cell Selection: Neomycin
• Sequence Data:
  ORF Nucleotide Sequence

  >MR231568 representing NM_011058
  Red=Cloning site Blue=ORF Green=Tags(s)
  
  TTTTGTAATACGACTCACTATAGGGCGGCCGGGAATTCGTCGACTGGATCCGGTACCGAGGAGATCTGCC
  GCCGCGATCGCC
  
  ATGGGGACCTCCCACCAGGTCTTTCTGGTCCTCAGCTGTCTCCTCACAGGGCCGGGCCTCATCTCCTGCC
  AGCTCTTATTACCCTCTATCCTCCCAAACGAGAATGAGAAGATTGTGCAGCTGAATTCGTCCTTTTCTCT
  GAGATGCGTTGGGGAGAGTGAAGTGAGCTGGCAGCACCCCATGTCTGAAGAAGACGACCCCAACGTGGAA
  ATCAGAAGTGAGGAGAACAACAGTGGCCTCTTTGTCACAGTGCTGGAAGTGGTTAACGCCTCCGCAGCCC
  ACACCGGATGGTACACTTGCTACTACAACCACACTCAGACGGATGAGAGTGAGATCGAAGGCAGGCACAT
  TTACATCTATGTACCAGACCCAGACATGGCCTTTGTGCCTCTCGGGATGACCGATTCCTTAGTCATCGTG
  GAAGAGGATGACTCTGCCATCATACCTTGCCGCACCACAGATCCGGAGACTCAAGTAACCTTGCACAATA
  ACGGGAGGCTGGTGCCTGCCTCCTATGACAGCAGGCAGGGCTTCAACGGAACCTTCAGCGTGGGGCCTTA
  CATCTGTGAGGCCACCGTCAAAGGGAGGACGTTCAAGACCAGCGAGTTTAATGTTTATGCCTTGAAAGCA
  ACGTCAGAACTGAATCTGGAGATGGACGCACGCCAGACTGTGTATAAGGCAGGAGAAACGATCGTGGTGA
  CCTGTGCCGTCTTTAACAACGAGGTCGTTGACCTGCAGTGGACTTACCCTGGAGAAGTGAGAAACAAAGG
  TATCACCATGCTGGAGGAGATCAAACTCCCATCCATCAAACTGGTGTACACTTTGACCGTCCCCAAGGCC
  ACGGTGAAGGACAGTGGAGAGTATGAATGTGCTGCCCGTCAGGCCACTAAAGAGGTCAAGGAAATGAAGA
  GAGTCACCATTTCTGTCCACGAGAAAGGCTTCGTCGAGATTGAGCCCACCTTTGGCCAGCTGGAAGCTGT
  CAACTTGCACGAAGTCAGAGAGTTCGTGGTGGAGGTGCAGGCCTACCCGACGCCCAGGATATCGTGGCTG
  AAGGACAACTTGACTCTGATAGAGAATCTCACCGAGATCACCACTGATGTACAGAAGAGTCAGGAAACAA
  GGTATCAGAGCAAATTAAAGCTGATCCGGGCTAAGGAAGAAGACAGTGGCCATTACACCATTATAGTTCA
  AAATGAGGATGACGTGAAGAGTTACACGTTTGAGCTGTCAACCCTAGTTCCTGCATCCATTTTGGATCTC
  GTAGATGACCACCATGGCTCTGGCGGGGGACAGACTGTGAGGTGTACAGCCGAAGGCACCCCTCTTCCAG
  AAATCGACTGGATGATCTGCAAGCATATTAAGAAATGTAATAATGACACTTCGTGGACAGTTTTGGCCAG
  CAATGTCTCAAATATTATCACAGAGCTCCCTCGCCGTGGCAGGAGTACCGTGGAGGGACGAGTGTCCTTC
  GCCAAAGTGGAAGAGACCATCGCAGTTCGATGCCTGGCAAAGAACAACCTCAGCGTTGTGGCCCGTGAGC
  TGAAGCTGGTGGCTCCCACTCTGCGATCTGAACTCACAGTGGCGGCCGCAGTGTTGGTGCTGTTGGTGAT
  TGTCATTGTCTCTCTCATCGTCCTGGTGGTCATTTGGAAGCAGAAACCACGGTATGAAATTCGCTGGAGG
  GTTATCGAGTCAATCAGCCCCGATGGACATGAGTATATCTATGTGGACCCCATGCAGTTGCCTTACGACT
  CCAGATGGGAATTCCCGAGAGATGGCCTCGTGCTTGGTCGGATTTTGGGATCCGGTGCATTTGGGAAAGT
  GGTCGAAGGTACAGCTTATGGATTAAGCCGGTCCCAACCTGTAATGAAGGTGGCTGTGAAGATGCTCAAA
  CCCACAGCCAGGTCTAGTGAAAAGCAGGCTCTCATGTCTGAGCTGAAGATAATGACTCACCTGGGGCCAC
  ATTTGAACATTGTGAACTTGCTGGGAGCCTGCACCAAGTCAGGTCCCATTTACATCATCACAGAATACTG
  CTTCTATGGGGATTTGGTCAACTACTTGCATAAGAACAGAGATAGCTTCATGAGCCAACACCCAGAGAAG
  CCGAAGAAAGACCTGGACATCTTTGGATTGAATCCTGCAGACGAGAGCACAAGAAGTTATGTGATTTTGT
  CTTTTGAAAATAACGGCGACTACATGGACATGAAGCAAGCTGATACCACACAGTATGTCCCCATGCTTGA
  AAGGAAAGAGGTTTCTAAATACTCTGACATCCAGAGATCGCTGTACGATCGGCCAGCCTCCTACAAGAAG
  AAATCCATGCTAGACTCAGAAGTCAAAAACCTCCTTTCGGACGATGACTCCGAGGGTCTGACTTTGCTGG
  ATCTATTGAGCTTCACCTATCAAGTTGCTCGAGGAATGGAATTTTTGGCTTCGAAAAATTGTGTCCACCG
  GGACCTGGCTGCCCGCAACGTCCTCCTGGCGCAAGGAAAAATTGTGAAGATCTGTGATTTTGGCCTGGCC
  AGAGACATCATGCACGATTCCAACTACGTGTCAAAAGGCAGTACTTTCCTGCCTGTGAAGTGGATGGCAC
  CCGAGAGCATCTTCGACAACCTCTACACCACGCTGAGTGACGTCTGGTCCTATGGCATTCTGCTCTGGGA
  AATCTTTTCCCTTGGTGGCACACCCTATCCTGGCATGATGGTCGATTCTACTTTCTACAATAAGATCAAG
  AGTGGATACCGGATGGCCAAACCTGACCATGCCACCAGTGAAGTCTATGAGATCATGGTGCAGTGCTGGA
  ACAGTGAGCCCGAGAAGAGACCCTCCTTCTACCACCTCAGCGAGATAGTGGAGAACCTGTTGCCGGGACA
  ATACAAGAAGAGTTATGAAAAGATTCACCTGGACTTCCTAAAGAGTGACCATCCGGCCGTGGCACGCATG
  CGGGTGGACTCTGATAATGCGTACATCGGTGTCACTTACAAAAATGAAGAGGATAAGCTGAAGGACTGGG
  AAGGTGGCCTGGACGAACAGAGACTGAGCGCTGACAGTGGCTACATCATCCCCCTGCCAGACATTGACCC
  TGTTCCAGAGGAGGAAGACCTGGGCAAGAGGAACAGACACAGCTCACAGACTTCGGAAGAGAGTGCCATC
  GAGACAGGTTCCAGTAGTTCCACCTTCATCAAGAGAGAGGACGAGACCATCGAGGACATCGACATGATGG
  ATGACATTGGTATAGATTCCTCGGACCTGGTGGAGGACAGCTTCCTG
  
  ACGCGTACGCGGCCGCTCGAGCAGAAACTCATCTCAGAAGAGGATCTGGCAGCAAATGATATCCTGGATT
  ACAAGGATGACGACGATAAGGTTTAA
  Protein Sequence

  >MR231568 representing NM_011058
  Red=Cloning site Green=Tags(s)
  
  MGTSHQVFLVLSCLLTGPGLISCQLLLPSILPNENEKIVQLNSSFSLRCVGESEVSWQHPMSEEDDPNVE
  IRSEENNSGLFVTVLEVVNASAAHTGWYTCYYNHTQTDESEIEGRHIYIYVPDPDMAFVPLGMTDSLVIV
  EEDDSAIIPCRTTDPETQVTLHNNGRLVPASYDSRQGFNGTFSVGPYICEATVKGRTFKTSEFNVYALKA
  TSELNLEMDARQTVYKAGETIVVTCAVFNNEVVDLQWTYPGEVRNKGITMLEEIKLPSIKLVYTLTVPKA
  TVKDSGEYECAARQATKEVKEMKRVTISVHEKGFVEIEPTFGQLEAVNLHEVREFVVEVQAYPTPRISWL
  KDNLTLIENLTEITTDVQKSQETRYQSKLKLIRAKEEDSGHYTIIVQNEDDVKSYTFELSTLVPASILDL
  VDDHHGSGGGQTVRCTAEGTPLPEIDWMICKHIKKCNNDTSWTVLASNVSNIITELPRRGRSTVEGRVSF
  AKVEETIAVRCLAKNNLSVVARELKLVAPTLRSELTVAAAVLVLLVIVIVSLIVLVVIWKQKPRYEIRWR
  VIESISPDGHEYIYVDPMQLPYDSRWEFPRDGLVLGRILGSGAFGKVVEGTAYGLSRSQPVMKVAVKMLK
  PTARSSEKQALMSELKIMTHLGPHLNIVNLLGACTKSGPIYIITEYCFYGDLVNYLHKNRDSFMSQHPEK
  PKKDLDIFGLNPADESTRSYVILSFENNGDYMDMKQADTTQYVPMLERKEVSKYSDIQRSLYDRPASYKK
  KSMLDSEVKNLLSDDDSEGLTLLDLLSFTYQVARGMEFLASKNCVHRDLAARNVLLAQGKIVKICDFGLA
  RDIMHDSNYVSKGSTFLPVKWMAPESIFDNLYTTLSDVWSYGILLWEIFSLGGTPYPGMMVDSTFYNKIK
  SGYRMAKPDHATSEVYEIMVQCWNSEPEKRPSFYHLSEIVENLLPGQYKKSYEKIHLDFLKSDHPAVARM
  RVDSDNAYIGVTYKNEEDKLKDWEGGLDEQRLSADSGYIIPLPDIDPVPEEEDLGKRNRHSSQTSEESAI
  ETGSSSSTFIKREDETIEDIDMMDDIGIDSSDLVEDSFL
  
  TRTRPLEQKLISEEDLAANDILDYKDDDDKV
• Restriction Sites: SgfI-MluI Cloning Scheme for this gene Plasmid Map
• ACCN: NM_011058
• ORF Size: 3267 bp
• OTI Disclaimer: Due to the inherent nature of this plasmid, standard methods to replicate additional amounts of DNA in E. coli are highly likely to result in mutations and/or rearrangements. Therefore, OriGene does not guarantee the capability to replicate this plasmid DNA. Additional amounts of DNA can be purchased from OriGene with batch-specific, full-sequence verification at a reduced cost. Please contact our customer care team at custsupport@origene.com or by calling 301.340.3188 option 3 for pricing and delivery.
  
  The molecular sequence of this clone aligns with the gene accession number as a point of reference only. However, individual transcript sequences of the same gene can differ through naturally occurring variations (e.g. polymorphisms), each with its own valid existence. This clone is substantially in agreement with the reference, but a complete review of all prevailing variants is recommended prior to use. More info
• OTI Annotation: This clone was engineered to express the complete ORF with an expression tag. Expression varies depending on the nature of the gene.

Reference Data

• RefSeq: NM_011058.1, NM_011058.2, NM_011058.3, NP_035188.2
• RefSeq Size: 6553
• RefSeq ORF: 3270
• Locus ID: 18595
• MW: 122.7 kDa
• Gene Summary: This gene encodes a member of the receptor tyrosine kinase family of proteins. Binding of platelet-derived growth factor protein ligands to this receptor triggers receptor dimerization and autophosphorylation, resulting in the activation of several downstream signaling pathways. Signaling through the encoded receptor plays a role in gastrulation and the development of nearly all organ systems. Mice lacking a functional copy of this gene reportedly exhibit defects in lung, skeleton, testis and the central nervous system, and die soon after birth. Alternative splicing and intronic polyadenylation of gene transcripts have been implicated in muscle regeneration and fibrosis in adult mice. [provided by RefSeq, Jan 2017]